Corrosivity of H2S-producing bacteria isolated from formation waters used in secondary crude-oil recovery

• Zoilabet Duque Universidad Politécnica de Madrid-España
• Eduardo Chicote Universidad Politécnica de Madrid-España
• Isabel Sarró Universidad Politécnica de Madrid-España
• Diego Moreno Universidad Politécnica de Madrid-España
• Matilde de Romero Universidad del Zulia-Venezuela
• Orlando Pérez Universidad del Zulia-Venezuela

Palabras clave: MIC, H2S, Non-SRB, SRB, water inyection, oilfield

Resumen

There have been problems in the water-injection systems of the oil industry due to Microbiologically Induced Corroosion (MIC), associated with the presence of Sulphate-Reducing Bacteria (SRB), which produce H2S. Several investigators consider that this is the principal cause of bacterial corrosion in natural-water storage and distribution systems. Given the fact that other groups of bacteria are not considered in current microbiological treatments and controls, it would be useful to investigate the presence of other anaerobic microorganisms that produce this aggressive metabolite or its derivatives in water-injection systems. This article reports on SRB and Non-SRB strains isolated from injection systems and identified by DNA sequencing, among them, Desulfovibrio termitidis (SRB) and Escherichia coli (Non-SRB). Evaluation of the activity and corrosivity of the two types of bacteria indicated that there was a significant difference in activity in the selective media, mainly that sulphide generation by the sulphate dissimilation process is much greater than that of the group that generates it by fermentation, as well as corrosivity on the carbon steel API 5L grade X65, as determined by open circuit potential, polarization resistance and weight loss during 60 hours' evaluation in selective media with no ferrous salts. Nevertheless, Scanning Electron Microscopy indicated biofilm development and localized attacks on the steel by both types of bacteria, which confirms the need for investigating and considering the role of these Non-SRB anaerobic groups so as to exercise better control over bacterial corrosion.