Detection of antibodies against Histoplasma capsulatum, Paracoccidioides complex and Sporothrix schenckii complex infection in Canis familiaris, using agar gel immunodiffusion assay. Caroní municipality. Bolívar state, Venezuela.
Detección de anticuerpos contra Histoplasma capsulatum, complejo Paracoccidioides y complejo Sporothrix schenckii en Canis familiaris, mediante la prueba de inmunodifusión en gel de agarosa. municipio Caroní, estado Bolívar, Venezuela.
Keywords:
dogs, histoplasmosis, paracoccidioidomycosis, serology, sporotrichosis
Abstract
The dimorphic fungi, Histoplasma capsulatum, Paracoccidioides spp. complex and Sporothrix schenckii complex are the causative agents of histoplasmosis, paracoccidioidomycosis and sporotrichosis, respectively. The aim of this study was to detect antibodies against H. capsulatum, Paracoccidioides spp. complex, and Sporothrix schenckii complex in domestic dogs (Canis familiaris) from the Caroní municipality, Bolívar state, Venezuela. A prospective, descriptive and cross-sectional study was carried out. Two hundred serum samples were randomly collected from the same number of dogs belonging to 10 localities of the Caroní municipality. Using the agarose gel immunodiffusion technique, specific antibodies against antigens of H. capsulatum, Paracoccidioides spp. complex, and Sporothrix schenckii complex were determined. Nine percent (n= 18) of the canines studied showed antibodies against H. capsulatum, 7.0% (n= 14) against Paracoccidioides spp.; 1.0% (n= 2) for both fungi and 1.5% (n= 3) for the Sporothrix schenckii complex; most of them were found in San Félix and Unare locations. The detection of specific antibodies against Histoplasma capsulatum, Paracoccidioides spp. complex and S. schenckii complex in dogs, suggests that these agents are present in the Caroní municipality. Probably, the people that inhabit this area have a similar exposure to these fungiand, therefore, the diagnosis of conditions occasioned by these agents should be considered in this geographical zone.
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References
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Ricci G, Mota FT, Wakamatsu A, Serafim RC, Borra RC, Franco M. Canine paracoccidioidomycosis. Med Mycol 2004; 42(4): 379-383.
Brömel C, Sykes JE. Histoplasmosis in dogs and cats. Clin Tech Small Anim Pract 2005; 20(4):227-232.
Madrid IM, Mattei AS, Fernandes CG, Nobre Mde O, Meireles MC. Epidemiological findings and laboratory evaluation of sporo-trichosis: a description of 103 cases in cats and dogs in southern Brazil. Mycopathologia 2012; 173(4):265-273.
Hayward JJ, Castelhano MG, Oliveira KC, Corey E, Balkman C, Baxter TL, Casal ML, Center SA, Fang M, Garrison SJ, Kalla SE, Korniliev P, Kotlikoff MI, Moise NS, Shannon LM, Simpson KW, Sutter NB, Todhunter RJ, Boyko AR. Complex disease and phenotype mapping in the domestic dog. Nat Commun 2016; 7:10460.
Ono MA, Kishima MO, Itano EN, Bracarense AP, Camargo ZP. Experimental paracoccidioidomycosis in dogs. Med Mycol 2003; 41(3):265-268.
Tyre E, Eisenbart D, Foley P, Burton S. Histoplasmosis in a dog from New Brunswick. Can Vet J 2007; 48(7):734-736.
Canteros CE, Madariaga MJ, Lee W, Rivas MC, Davel G, Iachini R. Agentes de micosis endémicas en un área rural de Argentina: estudio seroepidemiológico en perros. Rev Iberoam Micol 2010; 27(1):14–19.
Emmons CW. Histoplasmosis: Animal reservoirs and other sources in nature of the pathogenic fungus, Histoplasma. AJPH 1950; 40: 436-440.
Merino E, Rodríguez C. Histoplasmosis in a dog. (First case described in Venezuela). Mycopathol Mycol Appl 1961; 15(1):291-298.
Canteros CE, Iachini RH, Rivas MC, Vaccaro O, Madariaga J, Galarza, R. Snaiderman, L, Martínez M, Paladino M, Cicuttin G, Varela E, Alcoba E, Zuiani MF, Sahaza JH, Taylor ML, Davel G. Primer aislamiento de Histoplasma capsulatum de murciélago urbano Eumops bonariensis. Rev Argent Microbiol 2005; 37(1): 46-56.
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Montenegro MA, Santa Cruz AM, Sánchez Histoplasma capsulatum en glándula adrenal de comadreja overa (Didelphys albi-ventris). Rev Veter 2009; 20(1): 54-56.
Arias S, Suárez F, Álvarez I, Gutiérrez E, Castellanos L, Cardona L. Histoplasmosis meningitis in a dog: case report. Rev Med Vet 2010; 20:39-47.
Farias MR, Condas LAZ, Ribeiro MG, Bosco SM, Muro MD, Werner J, Raquel Cordeiro R, Bagagli E, Marques SA, Franco Paracoccidioidomycosis in a dog: Case report of generalized lymphadenomegaly. Mycopathologia 2011; 172:147-152.
Martínez D, Hernández R, Alvarado P, Mendoza M. Las micosis en Venezuela: casuística de los Grupos de Trabajo en Micología (1984-2010). Rev Iberoam Micol 2013; 30(1):39-46.
Rodrigues AM, de Melo Teixeira M, de Hoog GS, Schubach TM, Pereira SA, Fernandes GF, Bezerra LM, Felipe MS, de Camargo ZP. Phylogenetic analysis reveals a high prevalence of Sporothrix brasiliensis in feline sporotrichosis outbreaks. PLoS Negl Trop Dis 2013; 7(6):e2281.
Schubach A, Barros MB, Wanke B. Epidemic sporotrichosis. Curr Opin Infect Dis 2008; 21(2):129-133.
Organización Panamericana de la Salud (OPS). Sporothrix brasiliensis, un patógeno fúngico emergente, notable por su transmisión zoonótica y potencial epidémico para la salud humana y animal en las Américas [Serial en línea]. 2019. [Consultado el 1 de julio del 2020]. Disponible en URL: http:// www.someve.com.ar/index.php/ noticias- someve/interes-general/833-sporothrix- brasiliensis.html.
Silva JN, Passos SL, Menezes RC, Gremiao ID, Schubach TM, Oliveira JC, Figueiredo AB, Pereira SA. Diagnostic accuracy assessment of cytopathological examination of feline sporotrichosis. Med Mycol 2015; 53(8):880-884.
Cermeño JR, Cermeño JJ, Godoy G, Hernández I, Orellán Y, Blanco Y, Penna S, García L, Mender T, Gonsálvez M, López C, Hernández N, Longa I, Gottberg E, Basanta A, Castro M, Millán I, León W, Plaz F. Epidemiological study of paracoccidioidomycosis and histoplasmosis in a suburb of San Félix city, Bolívar state, Venezuela. Invest Clin 2009; 50(2):213-220.
Cermeño J, Hernández I, Cabello I, Orellán Y, Cermeño JJ, Albornoz R, Padrón E, Godoy G. Cryptococcus neoformans and Histoplasma capsulatum in dove’s (Columbia livia) excreta in Bolívar State, Venezuela. Rev Latinoam Microbiol 2006; 48(1):6-9.
Gómez G. Características del Municipio Caroní. [Serial en línea]. 2011. [Consultado el 1 de julio del 2020]. Disponible en URL: http://www.alsobocaroni.gob.ve/por- tal/index.php? option=com_content&view=article&id=59<emid=260.
Ouchterlony O. Antigen-antibody reactions in gels. Acta Pathol Microbiol Scand 1949; 26(4):507-515.
Camargo ZP, Unterkircher CS, Campoy SP, Travassos LR. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol 1988; 26:2147-2151.
Cordeiro RA, Coelho CG, Brilhante RS, Sidrim JJ, Castelo-Branco DS, Moura FB, Rocha FA, Rocha MFG. Serological evidence of Histoplasma capsulatum infection among dogs with leishmaniasis in Brazil. Acta Trop 2011; 119(2-3):203-205.
Silveira LH, Domingos IH, Kouchi K, Itano EN, Silva EA, Landgraf VO, Werneck SM, Camargo ZP, Ono MA. Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis. Mycopathologia 2006; 162:325-329.
Ono MA, Bracarense AP, Morais HS, Trapp SM, Belitardo DR, Camargo ZP. Canine paracoccidioidomycosis: A seroepidemiologic study. Med Mycol 2001; 39:277–282.
Teles AJ, Klafke GB, Cabana ÂL, Albano AP, Xavier MO, Meireles MC. Serological investigation into Paracoccidioides brasiliensis infection in dogs from Southern Rio Grande do Sul, Brazil. Mycopathologia 2016; 181(3-4):323-328.
Mendes JF, Klafke GB, Albano APN, Cabana ÂL, Teles AJ, de Camargo ZP, Xavier MO, Meireles MCA. Paracoccidioidomycosis infection in domestic and wild mammals by Paracoccidioides lutzii. Mycoses 2017; 60(6):402-406.
Fernandes Fontana FF, Santos CTB, Esteves FM, Rocha A, Fernandes GF, Amaral CC, Domingues MA, Camargo ZP, Silva-Vergara ML. Seroepidemiological survey of paracoccidioidomycosis infection among urban and rural dogs from Uberaba, Minas Gerais, Brazil. Mycopathologia 2010; 169:159-165.
Corte AC, Gennari SM, Labruna MB, Camargo LMA, Itano EN, Freire RL, Camargo ZP, Ono MA. Paracoccidioides brasiliensis infection in dogs from Western Brazilian Amazon. Pesq Vet Bras 2012; 32(7):649-652.
Alvarado P, Ostos A, Franquiz N, Roschman-González A, Zambrano E, Mendoza M. Diagnóstico serológico de la esporotricosis mediante el empleo del antígeno de micelio de Sporothrix schenckii sensu stricto. Invest Clín 2015; 56(2):111-122.
Silva-Ribeiro VL, Ferreira-da-Cruz MR, Wanke B, Galvao-Castro B. Canine histoplasmosis in Rio de Janeiro: natural and experimental infections. J Med Vet Mycol 1987; 25:319–322.
Mitchell M, Stara DR. Disseminated canine histoplasmosis: A clinical survey of 24 cases in Texas. Can Vet J 1989; 21:95–100.
Mendoza M, Brito A, Schaper D, Spooner VA, Alvarado P, Castro A, Fernández A. Evaluación de la técnica PCR anidada para el diagnóstico de la esporotricosis experimental. Rev Iberoam Micol 2012; 29(3):120–125.
Clemans JM, Deitz KL, Riedesel EA, Yaeger MJ, Legendre AM. Retroperitoneal pyo-granulomatous and fibrosing inflammation secondary to fungal infections in two dogs. J Am Vet Med Assoc 2011; 238(2):213-219.
Ader PL. Phycomycosis in fifteen dogs and two cats. J Am Vet Med Assoc 1997; 174: 1216-1223.
Assi M, Lakkis IE, Wheat LJ. Cross reactivity in the Histoplasma antigen enzyme immunoassay caused by sporotrichosis. Clin Vaccine Immunol 2011; 18(10):1781-2.
Almeida-Paes R, Pimenta MA, Pizzini CV, Monteiro PC, Peralta JM, Nosanchuk JD, Zancopé-Oliveira RM. Use of mycelial-phase Sporothrix schenckii exoantigens in an enzyme-linked immunosorbent assay for diagnosis of sporotrichosis by antibody detection. Clin Vaccine Immunol 2007; 14(3):244-249.
Ishizaki H, Nakamura Y, Wheat RW. Sero logical cross reactivity between Sporothrix schenckii and various unrelated fungi. Mycopathologia 1981; 73(2):65-68.
Camargo ZP. Serology of paracoccidioidomycosis. Mycopathologia 2008; 165:289–302.
Cano LE, Restrepo A. Predictive value of serologic tests in the diagnosis and follow up of patients with paracoccidioidomycosis. Rev Inst Med Trop São Paulo 1987; 29:276– 283.
Ricci G, Mota FT, Wakamatsu A, Serafim RC, Borra RC, Franco M. Canine paracoccidioidomycosis. Med Mycol 2004; 42(4): 379-383.
Brömel C, Sykes JE. Histoplasmosis in dogs and cats. Clin Tech Small Anim Pract 2005; 20(4):227-232.
Madrid IM, Mattei AS, Fernandes CG, Nobre Mde O, Meireles MC. Epidemiological findings and laboratory evaluation of sporo-trichosis: a description of 103 cases in cats and dogs in southern Brazil. Mycopathologia 2012; 173(4):265-273.
Hayward JJ, Castelhano MG, Oliveira KC, Corey E, Balkman C, Baxter TL, Casal ML, Center SA, Fang M, Garrison SJ, Kalla SE, Korniliev P, Kotlikoff MI, Moise NS, Shannon LM, Simpson KW, Sutter NB, Todhunter RJ, Boyko AR. Complex disease and phenotype mapping in the domestic dog. Nat Commun 2016; 7:10460.
Ono MA, Kishima MO, Itano EN, Bracarense AP, Camargo ZP. Experimental paracoccidioidomycosis in dogs. Med Mycol 2003; 41(3):265-268.
Tyre E, Eisenbart D, Foley P, Burton S. Histoplasmosis in a dog from New Brunswick. Can Vet J 2007; 48(7):734-736.
Canteros CE, Madariaga MJ, Lee W, Rivas MC, Davel G, Iachini R. Agentes de micosis endémicas en un área rural de Argentina: estudio seroepidemiológico en perros. Rev Iberoam Micol 2010; 27(1):14–19.
Emmons CW. Histoplasmosis: Animal reservoirs and other sources in nature of the pathogenic fungus, Histoplasma. AJPH 1950; 40: 436-440.
Merino E, Rodríguez C. Histoplasmosis in a dog. (First case described in Venezuela). Mycopathol Mycol Appl 1961; 15(1):291-298.
Canteros CE, Iachini RH, Rivas MC, Vaccaro O, Madariaga J, Galarza, R. Snaiderman, L, Martínez M, Paladino M, Cicuttin G, Varela E, Alcoba E, Zuiani MF, Sahaza JH, Taylor ML, Davel G. Primer aislamiento de Histoplasma capsulatum de murciélago urbano Eumops bonariensis. Rev Argent Microbiol 2005; 37(1): 46-56.
Murata Y, Sano A, Ueda Y, Inomata T, Takayama A, Poonwan N, Nanthawan M, Mikami Y, Miyaji M, Nishimura K, Kamei K. Molecular epidemiology of canine histoplasmosis in Japan. Med Mycol 2007; 45(3):233-247.
Montenegro MA, Santa Cruz AM, Sánchez Histoplasma capsulatum en glándula adrenal de comadreja overa (Didelphys albi-ventris). Rev Veter 2009; 20(1): 54-56.
Arias S, Suárez F, Álvarez I, Gutiérrez E, Castellanos L, Cardona L. Histoplasmosis meningitis in a dog: case report. Rev Med Vet 2010; 20:39-47.
Farias MR, Condas LAZ, Ribeiro MG, Bosco SM, Muro MD, Werner J, Raquel Cordeiro R, Bagagli E, Marques SA, Franco Paracoccidioidomycosis in a dog: Case report of generalized lymphadenomegaly. Mycopathologia 2011; 172:147-152.
Martínez D, Hernández R, Alvarado P, Mendoza M. Las micosis en Venezuela: casuística de los Grupos de Trabajo en Micología (1984-2010). Rev Iberoam Micol 2013; 30(1):39-46.
Rodrigues AM, de Melo Teixeira M, de Hoog GS, Schubach TM, Pereira SA, Fernandes GF, Bezerra LM, Felipe MS, de Camargo ZP. Phylogenetic analysis reveals a high prevalence of Sporothrix brasiliensis in feline sporotrichosis outbreaks. PLoS Negl Trop Dis 2013; 7(6):e2281.
Schubach A, Barros MB, Wanke B. Epidemic sporotrichosis. Curr Opin Infect Dis 2008; 21(2):129-133.
Organización Panamericana de la Salud (OPS). Sporothrix brasiliensis, un patógeno fúngico emergente, notable por su transmisión zoonótica y potencial epidémico para la salud humana y animal en las Américas [Serial en línea]. 2019. [Consultado el 1 de julio del 2020]. Disponible en URL: http:// www.someve.com.ar/index.php/ noticias- someve/interes-general/833-sporothrix- brasiliensis.html.
Silva JN, Passos SL, Menezes RC, Gremiao ID, Schubach TM, Oliveira JC, Figueiredo AB, Pereira SA. Diagnostic accuracy assessment of cytopathological examination of feline sporotrichosis. Med Mycol 2015; 53(8):880-884.
Cermeño JR, Cermeño JJ, Godoy G, Hernández I, Orellán Y, Blanco Y, Penna S, García L, Mender T, Gonsálvez M, López C, Hernández N, Longa I, Gottberg E, Basanta A, Castro M, Millán I, León W, Plaz F. Epidemiological study of paracoccidioidomycosis and histoplasmosis in a suburb of San Félix city, Bolívar state, Venezuela. Invest Clin 2009; 50(2):213-220.
Cermeño J, Hernández I, Cabello I, Orellán Y, Cermeño JJ, Albornoz R, Padrón E, Godoy G. Cryptococcus neoformans and Histoplasma capsulatum in dove’s (Columbia livia) excreta in Bolívar State, Venezuela. Rev Latinoam Microbiol 2006; 48(1):6-9.
Gómez G. Características del Municipio Caroní. [Serial en línea]. 2011. [Consultado el 1 de julio del 2020]. Disponible en URL: http://www.alsobocaroni.gob.ve/por- tal/index.php? option=com_content&view=article&id=59<emid=260.
Ouchterlony O. Antigen-antibody reactions in gels. Acta Pathol Microbiol Scand 1949; 26(4):507-515.
Camargo ZP, Unterkircher CS, Campoy SP, Travassos LR. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol 1988; 26:2147-2151.
Cordeiro RA, Coelho CG, Brilhante RS, Sidrim JJ, Castelo-Branco DS, Moura FB, Rocha FA, Rocha MFG. Serological evidence of Histoplasma capsulatum infection among dogs with leishmaniasis in Brazil. Acta Trop 2011; 119(2-3):203-205.
Silveira LH, Domingos IH, Kouchi K, Itano EN, Silva EA, Landgraf VO, Werneck SM, Camargo ZP, Ono MA. Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis. Mycopathologia 2006; 162:325-329.
Ono MA, Bracarense AP, Morais HS, Trapp SM, Belitardo DR, Camargo ZP. Canine paracoccidioidomycosis: A seroepidemiologic study. Med Mycol 2001; 39:277–282.
Teles AJ, Klafke GB, Cabana ÂL, Albano AP, Xavier MO, Meireles MC. Serological investigation into Paracoccidioides brasiliensis infection in dogs from Southern Rio Grande do Sul, Brazil. Mycopathologia 2016; 181(3-4):323-328.
Mendes JF, Klafke GB, Albano APN, Cabana ÂL, Teles AJ, de Camargo ZP, Xavier MO, Meireles MCA. Paracoccidioidomycosis infection in domestic and wild mammals by Paracoccidioides lutzii. Mycoses 2017; 60(6):402-406.
Fernandes Fontana FF, Santos CTB, Esteves FM, Rocha A, Fernandes GF, Amaral CC, Domingues MA, Camargo ZP, Silva-Vergara ML. Seroepidemiological survey of paracoccidioidomycosis infection among urban and rural dogs from Uberaba, Minas Gerais, Brazil. Mycopathologia 2010; 169:159-165.
Corte AC, Gennari SM, Labruna MB, Camargo LMA, Itano EN, Freire RL, Camargo ZP, Ono MA. Paracoccidioides brasiliensis infection in dogs from Western Brazilian Amazon. Pesq Vet Bras 2012; 32(7):649-652.
Alvarado P, Ostos A, Franquiz N, Roschman-González A, Zambrano E, Mendoza M. Diagnóstico serológico de la esporotricosis mediante el empleo del antígeno de micelio de Sporothrix schenckii sensu stricto. Invest Clín 2015; 56(2):111-122.
Silva-Ribeiro VL, Ferreira-da-Cruz MR, Wanke B, Galvao-Castro B. Canine histoplasmosis in Rio de Janeiro: natural and experimental infections. J Med Vet Mycol 1987; 25:319–322.
Mitchell M, Stara DR. Disseminated canine histoplasmosis: A clinical survey of 24 cases in Texas. Can Vet J 1989; 21:95–100.
Mendoza M, Brito A, Schaper D, Spooner VA, Alvarado P, Castro A, Fernández A. Evaluación de la técnica PCR anidada para el diagnóstico de la esporotricosis experimental. Rev Iberoam Micol 2012; 29(3):120–125.
Clemans JM, Deitz KL, Riedesel EA, Yaeger MJ, Legendre AM. Retroperitoneal pyo-granulomatous and fibrosing inflammation secondary to fungal infections in two dogs. J Am Vet Med Assoc 2011; 238(2):213-219.
Ader PL. Phycomycosis in fifteen dogs and two cats. J Am Vet Med Assoc 1997; 174: 1216-1223.
Assi M, Lakkis IE, Wheat LJ. Cross reactivity in the Histoplasma antigen enzyme immunoassay caused by sporotrichosis. Clin Vaccine Immunol 2011; 18(10):1781-2.
Almeida-Paes R, Pimenta MA, Pizzini CV, Monteiro PC, Peralta JM, Nosanchuk JD, Zancopé-Oliveira RM. Use of mycelial-phase Sporothrix schenckii exoantigens in an enzyme-linked immunosorbent assay for diagnosis of sporotrichosis by antibody detection. Clin Vaccine Immunol 2007; 14(3):244-249.
Ishizaki H, Nakamura Y, Wheat RW. Sero logical cross reactivity between Sporothrix schenckii and various unrelated fungi. Mycopathologia 1981; 73(2):65-68.
Camargo ZP. Serology of paracoccidioidomycosis. Mycopathologia 2008; 165:289–302.
Cano LE, Restrepo A. Predictive value of serologic tests in the diagnosis and follow up of patients with paracoccidioidomycosis. Rev Inst Med Trop São Paulo 1987; 29:276– 283.
Published
2021-09-07
How to Cite
Cermeño, J. R., Ortiz , J. C., & Quintero, A. K. (2021). Detection of antibodies against Histoplasma capsulatum, Paracoccidioides complex and Sporothrix schenckii complex infection in Canis familiaris, using agar gel immunodiffusion assay. Caroní municipality. Bolívar state, Venezuela.: Detección de anticuerpos contra Histoplasma capsulatum, complejo Paracoccidioides y complejo Sporothrix schenckii en Canis familiaris, mediante la prueba de inmunodifusión en gel de agarosa. municipio Caroní, estado Bolívar, Venezuela. Investigación Clínica, 62(3), 208-218. https://doi.org/10.22209/IC.v62n3a02
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Original Works
