Efeito dos níveis de salinidade na atividade antifúngica do óleo essencial de Thymus contra Fusarium oxysporum

• Boualem Boumaaza Department of Agronomy, University of Abdelhamid Ibn Badis, BP 300, 27000, Mostaganem, Algeria. University of Ibn khaldounTiaret. BP 78 zaâroura 14000, Tiaret, Algéria. https://orcid.org/0000-0003-0993-2931
• M’hamed Benada University of Ahmed Zabana. BP 48000 Bormadia Relizane. https://orcid.org/0000-0002-6319-8822
• Sofiane Boudalia Laboratory of Biology, Water and Environment (LBEE), University of 8th May, 1945 Guelma, BP 401 24000 Guelma, Algeria. https://orcid.org/0000-0002-7729-185X
• Ibrahim E. Benzohra Scientific and Technical Research Center on Arid Regions (CRSTRA) BP. Box 1682 RP 07000, Biskra, Algeria. Conservation Laboratory Wetlands. https://orcid.org/0000-0002-4234-9302
• Abdelhamid Gacemi INRA Station de Recherche Hmadna. BP 48017 Relizane. https://orcid.org/0000-0003-0993-2931
• Omar Khaladi Laboratory of Biology, Water and Environment (LBEE), University of 8th May, 1945 Guelma, BP 401 24000 Guelma, Algeria. https://orcid.org/0000-0002-2861-0295
• Mohamed Benkhelifa Department of Agronomy, University of Abdelhamid Ibn Badis, BP 300, 27000, Mostaganem, Algeria. https://orcid.org/0000-0003-0236-2446

Palavras-chave: Actividad antimicrobiana, biocontrole, extratos de plantas, planta medicinal, cloreto de sódio

Resumo

O tomilho (Thymus sp.), planta medicinal da família Lamiaceae, utilizada na medicina tradicional, contém uma grande variedade de componentes medicinais ativos, em sua grande maioria uma mistura bastante complexa de Timol, ρ-Cymene, γ-Terpinene, β-Caryophyllen, etc. O presente estudo foi avaliar a eficácia do extrato de Thymus vulgaris contra Fusarium oxysporum f. sp. radicis-lycopersici sob condições salinas, assumindo solos com alto teor de sal das regiões áridas. O óleo essencial por extraído pe la técnica de hidrodestilação utilizando aparelho de Clevenger. O composto de óleo essencial por identificado por análise GC-MS. Atividade antifúngica do óleo esencial contra Fusarium oxysporum f. sp. radicis-lycopersici por investigado pelo método de diluição em agar. O principais constituintes do óleo esencial de tomilho foram timol (76.96 %), ρ-cimeno (9.89 %) e γ-Terpineno (1.92 %). O óleo esencial de Timo apresentou alta ativida de in vitro, no control da germinação de conídios e do crescimento micelial. No entanto, o óleo não por significativamente ativo contra produção de esporos meio de salinidade. Os resultados sugerem que o crescimento micelial por estimulado em concentrações de 0,6 – 1.5 %. Pelo contrário, reduziu significativamente na concentração mais alta (2 %). A aplicação de NaCl causou um aumento significativo na produção de conidiogênese em várias concentrações testadas. A germinação dos conídios for levemente inibida pelo cloreto de sódio somente quando a concentração for de 2 %. Os resultados este estudo indicaque a salinidade diminui a eficácia do óleo esencialcontra o patógeno.

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