Biological control of Ralstonia solanacearum and its effect on the vegetative growth of organic banana
Keywords:
Musa AAA, Trichoderma, Bacillus, ADMF®, Ralstonia solanacearum, AUDPC
Abstract
The banana (Musa AAA) is one of the main economically important crops worldwide. Currently, it faces a serious problem of plant death caused by the bacterium Ralstonia solanacearum (Smith) variety 2. The objective of this research was to evaluate the effect of biological control of Moko disease and its relationship with the vegetative growth of banana seedlings under field conditions. Four treatments were employed: absolute control (T0), Trichoderma spp. (T2), Bacillus spp. (Bio-remedy) (T3), and ADMF® (T4) with three replications in a completely randomized block design. The incubation period, the incidence percentage, and the area under the disease progress curve (AUDPC) were evaluated, along with plant survival and height, pseudostem diameter, and leaf emission rate. At 113 days after transplanting (dat), treatments T1, T2, and T3 showed higher seedling survival. Plant height (111-145 cm), pseudostem diameter (7.43-11.28 cm), and leaf emission rate (11.74-13.15 leaves) exhibited significant differences among treatments. Treated plants showed the lowest AUDPC (between 576.1 and 1435.4 units) compared to untreated plants (3156.55 units). Trichoderma, Bacillus, and ADMF® were demonstrated to reduce disease incidence and promote banana vegetative growth, proving to be viable sustainable alternatives for this crop.
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References
Agamez Ramos, E. Y., Zapata Navarro, R. I., Oviedo Zumaqué, L. E., & Barrera Violeth, J. L. (2008). Evaluation of substrates and fermentation solid process for spores production of Trichoderma sp. Revista Colombiana de Biotecnología, 10(2), 23-34. https://www.redalyc.org/articulo.oa?id=77610204
Avozani, A., Tumelero, A. I., Baldiga Tonin , R., Denardin, N., Gomes Silva, A., & Garcés-Fiallos, F. R. (2022). Biological control of Corynespora cassicola and Drechslera tritici-repentis. Revista de Agricultura Neotropical, 9(4), e7111. https://doi.org/10.32404/rean.v9i4.7111
Bautista-Montealegre, L. G., Bolaños-Benavides, M. M., Abaunza-González, C. A., Arguelles-Cárdenas, J. H., & Forero-Camacho, C. A. (2016). Moko de plátano y su relación con propiedades físicas y químicas en suelos del departamento de Quindío Colombia. Revista Colombiana de Ciencias Hortícolas, 10(2), 273-283. https://doi.org/10.17584/rcch.2016v10i2.5066
Brunda, N. B., Chirag, D., Jilen, M., & Menaka, M. (2023). Effect of blending proportion on sensory appeal of the blended squash using banana pseudostem sap with mango, papaya and Aloe vera. IJCS, 11(1), 06-11. https://www.chemijournal.com/archives/2023/vol11issue1/PartA/10-6-32-941.pdf
Castaño-Zapata, J. (1989). Estandarización de la estimación de daños causados por hongos, bacterias y nematodos en fríjol (Phaseolus vulgaris L.). Fitopatología Colombiana, 13(1), 9-19.
Castillo, T. (2023). Effect of shade on the severity of Moko (Ralstonia solanacearum) on Guineo (Musa balbisiana ABB). European Journal of Agriculture and Food Sciences, 5(1), 1-5. https://doi.org/10.24018/ejfood.2023.5.1.611
Ceballos, G., Álvarez, E., & Bolaños, M. M. (2014). Reduction in populations of Ralstonia solanacearum race 2 in plantain (Musa AAB Simmonds) with extracts from Trichoderma spp. and antagonistic bacteria. Acta Agronomica, 63(1), 83-90. https://doi.org/10.15446/acag.v63n1.43121
Chávez-Arteaga, K.T., Cedeño-Moreira, Á.V., Canchignia-Martínez, H.F., & Garcés Fiallos, F.R. (2022). Candidate rhizobacteria as plant growth-promoters and root-knot nematode controllers in tomato plants. Scientia Agropecuaria, 13(4), 423-432. https://doi.org/10.17268/sci.agropecu.2022.038
Creencia Armi, R., Alcantara, E. P., Opulencia, R. B., Diaz, M. G. Q., & Monsalud, R. G. (2022). A preliminary screening of philippine mangrove soil bacteria exhibit suppression of Ralstonia solanacearum (Smith) Yabuuchi et al. causing moko disease of banana (Musa acuminata Cavendish subgroup) under laboratory conditions. Journal of the International Society for Southeast Asian Agricultural Sciences, 28(1), 135-148. http://issaasphil.org/wp-content/uploads/2022/06/11.-Creencia-et-al-2022-Mangrove-soil-bacteria-FINAL.pdf
Crespo-Clas, Á.M., Canchignia-Martínez, H.F., & Garcés-Fiallos, F.R. (2023). Nematodes and root system are affected by rhizobacterial consortium in the third generation of commercial banana plants. Revista de Agricultura Neotropical, 10(3), e7725. https://doi.org/10.32404/rean.v10i3.7725
Debnath, S., Khan, A. A., Das, A., Murmu, I., Khan, A., & Mandal, K. K. (2019). Genetic Diversity in Banana. 217-241. https://doi.org/10.1007/978-3-319-96454-6_8
Fernandes Domingues Duarte, C., Cecato, U., Trento Biserra, T., Mamédio, D., & Galbeiro, S. (2020). Azospirillum spp. en gramíneas y forrajeras. Revisión. Rev. Mex. Cienc. Pecu., 11(1), 223-240. https://doi.org/10.22319/rmcp.v11i1.4951
He, L. Y., Sequeira, L., & Kelman, A. (1983). Characteristics of strains of Pseudomonas solanacearum from China. Plant Disease, 67, 1357-1361. https://www.apsnet.org/publications/plantdisease/backissues/Documents/1983Articles/PlantDisease67n12_1357.PDF
Leite Pais, A. K., Silva dos Santos, L. V., Rodrigues Albuquerque, G. M., Gomes de Farias, A. R., Silva Junior, W. J., de Queiroz Balbino, V., Freire Silva, A. M., Siqueira da Gama, M. A., & Barbosa de Souza, E. (2022). Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants. Genetics and Molecular Biology, 45, 4, e20220038. https://doi.org/10.1590/1678-4685-GMB-2022-0038
Li, X., Li, K., Zhou, D., Zhang, M., Qi, D., Jing, T., Zang, X., Qi, C., Wang, W., Xie, J. (2021). Biological control of banana wilt disease caused by Fusarium oxyspoum f. sp. cubense using Streptomyces sp. H4. Biological Control, 155, 104524. https://doi.org/10.1016/j.biocontrol.2020.104524
Quispe-Quispe, E., Moreira-Morrillo, A.A., & Garcés-Fiallos, F.R. (2022). Una revisión sobre biocontroladores de Phytophthora capsici y su impacto en plantas de Capsicum: Una perspectiva desde el exterior al interior de la planta. Scientia Agropecuaria, 13(3), 275-289. https://doi.org/10.17268/sci.agropecu.2022.025
Ramírez, M., Neuman, B. W., & Ramírez, C. A. (2020). Bacteriophages as promising agents for the biological control of Moko disease (Ralstonia solanacearum) of banana. Biological Control, 149, 104238. https://doi.org/10.1016/j.biocontrol.2020.104238
Sabando-Ávila, F., Molina-Atiencia, L.M., & Garcés-Fiallos, F.R. (2017). Trichoderma harzianum en pre-trasplante aumenta el potencial agronómico del cultivo de piña. Revista Brasileira De Ciências Agrárias, 12(4), 410-414. https://doi.org/10.5039/agraria.v12i4a5468
Santos, H. G., Jacomine, P. K. T., Anjos, L. H. C., Oliveira, V. A., Oliveira, J. B., Coelho, M. R., Lumbreras, J. F., & Cunha, T. J. F. (2013). Sistema brasileiro de classificação de solos. 3. ed. Rev. Ampl. Rio de Janeiro: Embrapa Solos.
Shaner, G., and Finney R. E. (1977). The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology, 67, 1051-1056. https://doi.org/10.1094/Phyto-67-1051
Soil SurveyStaff. (2014). Keys to soil taxonomy. Washington: Natural Resources Conservation Service and Agriculture Department.
Valencia, L., Álvarez, E., & Castaño, J. (2014). Resistencia de treinta y cuatro genotipos de platano (Musa AAB) y banano (Musa AAA) a cinco cepas de Ralstonia solanacearum Raza 2 (Smith). Agronomía, 22(2), 21-34. http://agronomia.ucaldas.edu.co/downloads/Agronomia 22(2)_3.pdf
Vargas-Calvo, A., Acuña-Chinchilla, P., & Valle-Ruiz, H. (2015). La emisión foliar en plátano y su relación con la diferenciación floral. Agronomía Mesoamericana, 26(1), 120-128. https://doi.org/10.15517/am.v26i1.16935
Villegas-Escobar, V., González-Jaramillo, L.M., Ramírez, M., Moncada, R.N., Sierra-Zapata, L., Orduz, S., & Romero-Tabarez, M. (2018). Lipopeptides from Bacillus sp. EA-CB0959: Active metabolites responsible for in vitro and in vivo control of Ralstonia solanacearum. Biological Control, 125, 20-28. https://doi.org/10.1016/j.biocontrol.2018.06.005
Zhou, Y., Yang, L., Wang, J., Guo, L., & Huang, J. (2021). Synergistic effect between Trichoderma virens and Bacillus velezensis on the control of tomato bacterial wilt disease. Horticulturae, 7(11), 439. https://doi.org/10.3390/horticulturae7110439
Avozani, A., Tumelero, A. I., Baldiga Tonin , R., Denardin, N., Gomes Silva, A., & Garcés-Fiallos, F. R. (2022). Biological control of Corynespora cassicola and Drechslera tritici-repentis. Revista de Agricultura Neotropical, 9(4), e7111. https://doi.org/10.32404/rean.v9i4.7111
Bautista-Montealegre, L. G., Bolaños-Benavides, M. M., Abaunza-González, C. A., Arguelles-Cárdenas, J. H., & Forero-Camacho, C. A. (2016). Moko de plátano y su relación con propiedades físicas y químicas en suelos del departamento de Quindío Colombia. Revista Colombiana de Ciencias Hortícolas, 10(2), 273-283. https://doi.org/10.17584/rcch.2016v10i2.5066
Brunda, N. B., Chirag, D., Jilen, M., & Menaka, M. (2023). Effect of blending proportion on sensory appeal of the blended squash using banana pseudostem sap with mango, papaya and Aloe vera. IJCS, 11(1), 06-11. https://www.chemijournal.com/archives/2023/vol11issue1/PartA/10-6-32-941.pdf
Castaño-Zapata, J. (1989). Estandarización de la estimación de daños causados por hongos, bacterias y nematodos en fríjol (Phaseolus vulgaris L.). Fitopatología Colombiana, 13(1), 9-19.
Castillo, T. (2023). Effect of shade on the severity of Moko (Ralstonia solanacearum) on Guineo (Musa balbisiana ABB). European Journal of Agriculture and Food Sciences, 5(1), 1-5. https://doi.org/10.24018/ejfood.2023.5.1.611
Ceballos, G., Álvarez, E., & Bolaños, M. M. (2014). Reduction in populations of Ralstonia solanacearum race 2 in plantain (Musa AAB Simmonds) with extracts from Trichoderma spp. and antagonistic bacteria. Acta Agronomica, 63(1), 83-90. https://doi.org/10.15446/acag.v63n1.43121
Chávez-Arteaga, K.T., Cedeño-Moreira, Á.V., Canchignia-Martínez, H.F., & Garcés Fiallos, F.R. (2022). Candidate rhizobacteria as plant growth-promoters and root-knot nematode controllers in tomato plants. Scientia Agropecuaria, 13(4), 423-432. https://doi.org/10.17268/sci.agropecu.2022.038
Creencia Armi, R., Alcantara, E. P., Opulencia, R. B., Diaz, M. G. Q., & Monsalud, R. G. (2022). A preliminary screening of philippine mangrove soil bacteria exhibit suppression of Ralstonia solanacearum (Smith) Yabuuchi et al. causing moko disease of banana (Musa acuminata Cavendish subgroup) under laboratory conditions. Journal of the International Society for Southeast Asian Agricultural Sciences, 28(1), 135-148. http://issaasphil.org/wp-content/uploads/2022/06/11.-Creencia-et-al-2022-Mangrove-soil-bacteria-FINAL.pdf
Crespo-Clas, Á.M., Canchignia-Martínez, H.F., & Garcés-Fiallos, F.R. (2023). Nematodes and root system are affected by rhizobacterial consortium in the third generation of commercial banana plants. Revista de Agricultura Neotropical, 10(3), e7725. https://doi.org/10.32404/rean.v10i3.7725
Debnath, S., Khan, A. A., Das, A., Murmu, I., Khan, A., & Mandal, K. K. (2019). Genetic Diversity in Banana. 217-241. https://doi.org/10.1007/978-3-319-96454-6_8
Fernandes Domingues Duarte, C., Cecato, U., Trento Biserra, T., Mamédio, D., & Galbeiro, S. (2020). Azospirillum spp. en gramíneas y forrajeras. Revisión. Rev. Mex. Cienc. Pecu., 11(1), 223-240. https://doi.org/10.22319/rmcp.v11i1.4951
He, L. Y., Sequeira, L., & Kelman, A. (1983). Characteristics of strains of Pseudomonas solanacearum from China. Plant Disease, 67, 1357-1361. https://www.apsnet.org/publications/plantdisease/backissues/Documents/1983Articles/PlantDisease67n12_1357.PDF
Leite Pais, A. K., Silva dos Santos, L. V., Rodrigues Albuquerque, G. M., Gomes de Farias, A. R., Silva Junior, W. J., de Queiroz Balbino, V., Freire Silva, A. M., Siqueira da Gama, M. A., & Barbosa de Souza, E. (2022). Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants. Genetics and Molecular Biology, 45, 4, e20220038. https://doi.org/10.1590/1678-4685-GMB-2022-0038
Li, X., Li, K., Zhou, D., Zhang, M., Qi, D., Jing, T., Zang, X., Qi, C., Wang, W., Xie, J. (2021). Biological control of banana wilt disease caused by Fusarium oxyspoum f. sp. cubense using Streptomyces sp. H4. Biological Control, 155, 104524. https://doi.org/10.1016/j.biocontrol.2020.104524
Quispe-Quispe, E., Moreira-Morrillo, A.A., & Garcés-Fiallos, F.R. (2022). Una revisión sobre biocontroladores de Phytophthora capsici y su impacto en plantas de Capsicum: Una perspectiva desde el exterior al interior de la planta. Scientia Agropecuaria, 13(3), 275-289. https://doi.org/10.17268/sci.agropecu.2022.025
Ramírez, M., Neuman, B. W., & Ramírez, C. A. (2020). Bacteriophages as promising agents for the biological control of Moko disease (Ralstonia solanacearum) of banana. Biological Control, 149, 104238. https://doi.org/10.1016/j.biocontrol.2020.104238
Sabando-Ávila, F., Molina-Atiencia, L.M., & Garcés-Fiallos, F.R. (2017). Trichoderma harzianum en pre-trasplante aumenta el potencial agronómico del cultivo de piña. Revista Brasileira De Ciências Agrárias, 12(4), 410-414. https://doi.org/10.5039/agraria.v12i4a5468
Santos, H. G., Jacomine, P. K. T., Anjos, L. H. C., Oliveira, V. A., Oliveira, J. B., Coelho, M. R., Lumbreras, J. F., & Cunha, T. J. F. (2013). Sistema brasileiro de classificação de solos. 3. ed. Rev. Ampl. Rio de Janeiro: Embrapa Solos.
Shaner, G., and Finney R. E. (1977). The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology, 67, 1051-1056. https://doi.org/10.1094/Phyto-67-1051
Soil SurveyStaff. (2014). Keys to soil taxonomy. Washington: Natural Resources Conservation Service and Agriculture Department.
Valencia, L., Álvarez, E., & Castaño, J. (2014). Resistencia de treinta y cuatro genotipos de platano (Musa AAB) y banano (Musa AAA) a cinco cepas de Ralstonia solanacearum Raza 2 (Smith). Agronomía, 22(2), 21-34. http://agronomia.ucaldas.edu.co/downloads/Agronomia 22(2)_3.pdf
Vargas-Calvo, A., Acuña-Chinchilla, P., & Valle-Ruiz, H. (2015). La emisión foliar en plátano y su relación con la diferenciación floral. Agronomía Mesoamericana, 26(1), 120-128. https://doi.org/10.15517/am.v26i1.16935
Villegas-Escobar, V., González-Jaramillo, L.M., Ramírez, M., Moncada, R.N., Sierra-Zapata, L., Orduz, S., & Romero-Tabarez, M. (2018). Lipopeptides from Bacillus sp. EA-CB0959: Active metabolites responsible for in vitro and in vivo control of Ralstonia solanacearum. Biological Control, 125, 20-28. https://doi.org/10.1016/j.biocontrol.2018.06.005
Zhou, Y., Yang, L., Wang, J., Guo, L., & Huang, J. (2021). Synergistic effect between Trichoderma virens and Bacillus velezensis on the control of tomato bacterial wilt disease. Horticulturae, 7(11), 439. https://doi.org/10.3390/horticulturae7110439
Published
2024-05-22
How to Cite
Ramos-Veintimilla, M., Zambrano-Gavilanes, F., Solís-Hidalgo, K., Garcés-Fiallos, F., Quimi Arce, V., & Sánchez-Urdaneta, A. (2024). Biological control of Ralstonia solanacearum and its effect on the vegetative growth of organic banana. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 41(2), e244116. Retrieved from https://produccioncientificaluz.org/index.php/agronomia/article/view/42168
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Crop Production
Copyright (c) 2024 Mario Ramos-Veintimilla, Freddy Zambrano-Gavilanes, Karina Solís-Hidalgo, Felipe R. Garcés-Fiallos, Víctor Quimi Arce, Adriana Beatriz Sánchez-Urdaneta
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