Molecular variation of lipoxygenase-associated genes in grain of commercial Mexican soybean cultivars

• Mónica López Laboratorio de Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, México https://orcid.org/0000-0002-3921-4335
• Ana Sifuentes Laboratorio de Biotecnología Animal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, México https://orcid.org/0000-0002-3867-9886
• Francisco Paredes Universidad Autónoma de Tamaulipas. IA-UAM, Mante, Tamaulipas, México https://orcid.org/0000-0003-2661-6607
• Nicolás Maldonado INIFAP-Campo Experimental Las Huastecas. Carretera Tampico-Mante, km 55. Villa Cuauhtémoc, Altamira, Tamaulipas, México https://orcid.org/0000-0002-2245-0884
• Homar Gill Laboratorio de Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, México https://orcid.org/0000-0002-3357-2940

Keywords: soybean grain, lipoxygenase, Lox genes, single nucleotide polymorphisms

Abstract

Lipoxygenase enzymes encoded by the Lox1, Lox2 and Lox3 genes play a crucial role in soybean grain, particularly in the development of off-flavors. Understanding molecular variation within Lox genes is essential for the improvement of soybean organoleptic traits. This study investigated the genetic variation in the internal regions of the Lox1, Lox2, and Lox3 genes in mature grain of commercially grown soybean cultivars in Mexico. Genomic DNA from a diverse panel of Mexican soybean cultivars was analyzed using resequencing techniques and in-silico analysis. Single nucleotide polymorphisms (SNP) within the Lox1, Lox2, and Lox3 genes were identified and characterized. The findings indicated that Lox3 gene displayed lower genetic variability compared to Lox1 and Lox2 genes, specifically, was identified a total of 26 SNPs in the Lox1 gene, 11 SNPs in the Lox2 gene, and 5 SNPs in the Lox3 gene among the examined cultivars. A non-synonymous SNP variant of the C/C genotype located in exon 6 of the Lox2 gene was associated with a destabilizing effect on the lipoxygenase 2 enzyme in the Guayparime S-10 and Huasteca 300 cultivars. These findings provide insights into the molecular variation of lipoxygenase-associated genes in Mexican soybean cultivars.

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