Correlation between bacterial type/bacterial quantity and bone loss detected by cone beam computed tomography (CBCT) in primary endodontic infections.

Correlación entre el tipo y cantidad de bacterias y la pérdida ósea detectada por Tomografía Computarizada de Haz Cónico (CBCT) en infecciones endodóncicas primarias.

• Ricardo Oliva Rodríguez Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. https://orcid.org/0000-0002-1797-1773
• Georgina Yaretzy Rangel Galván Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. https://orcid.org/0000-0003-3883-8258
• Ana María González Amaro Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. https://orcid.org/0000-0002-6375-9642
• Francisco Javier Gutiérrez Cantú Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. https://orcid.org/0000-0001-7220-5791
• Abraham Israel Muñoz Ruiz Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. https://orcid.org/0000-0003-2522-3617
• José Obed García Cortés Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. https://orcid.org/0000-0002-7990-7312
• Jairo Mariel Cárdenas Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. https://orcid.org/0000-0002-4733-7271

DOI: https://doi.org/10.54817/IC.v64n1a01

Keywords: bone loss, cone beam, primary endodontic infection

Abstract

Periapical lesions (PL) of endodontic origin are one of the most common pathological conditions that affect peri-radicular tissues. The main objective of this study was to evaluate the amount and species of microorganisms isolated from necrotic pulps, establish a correlation between these and the size of periapical lesions, and how the amount and species of microorganisms decreased with non-surgical root canal treatment. Twenty-seven patients with a clinical diagnosis of dental pulp necrosis and chronic periapical lesions were selected; a Cone Beam Computed Tomography (CBCT) and microbial samples of the root canal system were taken previous to a disinfection protocol, a post-instrumentation/disinfection protocol, and a post-medication placement. Samples were processed for colony-forming unit (CFU) counting, Gram staining technique, and bacterial identification by the API-20 Strep/API-20A system. The API system identified 21 species of microorganisms in the pre- instrumentation samples, 11 species in the post-instrumentation samples, and 11 in the post-medication samples. There was a correlation coefficient of 0.598% between the initial size of the lesion and the number of bacteria, with a coefficient of determination up to 35.7%, a correlation coefficient of 0.486% and a determination coefficient of 23.6% between the size of the periapical lesion and the number of CFUs. This study contributes to the knowledge of the amount and species of microorganisms isolated and identified from necrotic pulps, establishes a correlation between the amount and species of microorganisms and the size of the periapical lesions, and shows how the decrease of microorganisms contributes to the healing of PL, corroborating the importance of an adequate disinfection protocol.

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