Un método simple para la detección de mutaciones en el aminoácido 452 de la proteína de la Espiga del SARS-CoV-2, usando análisis de enzimas de restricción.

A simple method for detection of mutations in amino acid 452 of the Spike protein of SARS-CoV-2 using restriction enzyme analysis.

• Rossana C. Jaspe Instituto Venezolano de Investigaciones Científicas
• Yoneira Sulbaran Instituto Venezolano de Investigaciones Científicas
• Mariana Hidalgo Instituto Venezolano de Investigaciones Científicas
• Carmen L. Loureiro Instituto Venezolano de Investigaciones Científicas
• Zoila C. Moros Instituto Venezolano de Investigaciones Científicas
• Domingo J. Garzaro Instituto Venezolano de Investigaciones Científicas
• Héctor R. Rangel Instituto Venezolano de Investigaciones Científicas
• Flor H. Pujol Instituto Venezolano de Investigaciones Científicas

DOI: https://doi.org/10.22209/IC.v62n4a07

Keywords: COVID-19, SARS-CoV-2, Delta Variant of Concern, RFLP, rapid screening, mutation L452R

Abstract

Variants of Concern or Interest of SARS-CoV-2 (VOC or VOI), the coronavirus responsible for COVID-19, have emerged in several countries. Mutations in the amino acid 452 of the Spike protein are particularly important and associated with some of these variants: L452R, present in Delta VOC, and L452Q, present in Lambda VOI. These mutations have been associated with both increased infectivity and evasion of protective immune response. A search on GISAID to detect the number of sequences harboring the L452R mutation and the frequency of Delta VOC among them, showed that since August 2021, most of these sequences belong to the Delta VOC. Restriction enzyme analysis is proposed as a rapid method to detect L452R. A small amplicon from the Spike gene was digested with MspI. A 100% concordance was observed between digestion and sequencing results. The mutation L452Q can also be detected by restriction analysis, allowing the identification of putative Lambda VOIs. The proposed methodology, which allows screening of a great number of samples, could provide a faster information on the prevalence of Delta VOC cases.

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