Associação da expressão gênica com metabólitos sanguíneos e perfil de ácidos graxos em cordeiros
Palavras-chave:
expressão gênica, associação gênica, metabolismo lipídico, cabrito
Resumo
Alguns subprodutos agrícolas podem ser utilizados como alternativas viáveis na alimentação animal. Portanto, foi avaliado o efeito da inclusão de farinha de abacate e óleo de girassol no perfil de ácidos graxos, metabólitos sanguíneos e na expressão de genes associados ao metabolismo lipídico em cordeiros. Foram avaliados três tratamentos: 0 % controle, 10 % farinha de abacate e 10 % óleo de girassol por 84 dias. Verificou-se que a inclusão da farinha de abacate na dieta aumentou a quantidade de glicemia e também de globulina, mas diminuiu a creatinina e a Transaminase Glutâmica Oxaloacética, em comparação à adição de óleo de girassol onde diminuiu o teor de colesterol, triglicerídeos e VLDL. G. Não houve diferença para o percentual de gordura muscular. A concentração dos ácidos graxos C20:0, C20:1 e C23:0 foi menor com a farinha de abacate, sem afetar os valores de MUFA e UFA, a relação AGL/SFA e o índice de saturação. A inclusão da farinha de abacate causou alteração no expressão dos genes ACACA, FASN, SCD, FASBP3, PPARG e SREBF1 no músculo Longisimus thoracis, houve associação positiva entre glicose e o gene FASBP3, também de HDL com o gene PPARG, MUFA, proteínas e índices de dessaturação de gordura ácidos foram associados a genes como SCD, FASN, SREBF1 e ACACA. A inclusão da farinha de abacate é uma alternativa para modificar a expressão genética, também para reduzir os valores de colesterol de densidade muito baixa no sangue.
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Referências
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Astuti, D. A., Babab, A. S. & Wibawanc, I. W. T. (2011). Rumen Fermentation, Blood Metabolites, and Performance of Sheep Fed Tropical Browse Plants. Media Peternakan, 34(3), 201-206. https://doi.org/10.5398/medpet.2011.34.3.201
Avellanet, R., Cuenca, R., Pastor, J. y Jordana, J. (2007). Parámetros hematológicos y bioquímico clínicos en la raza ovina xisqueta. Archivos de Zootecnia, 56(Sup. 1), 497-501. https://www.redalyc.org/pdf/495/49509922.pdf
de Castro Budel, J. C., Gomes de Castro, V. C., Mota de Souza, S., de Souza Nahúm, B., Correa Barbosa, A. V., Silva Rodrigues, L., Berndt, A., Navajas Rennó, L., de Morais, E., Rodrigues da Silva, J. A., de Carvalho Rodrigues, T. C. G., Maciel e Silva, A. G. & Lourenço-Junior, J. B. (2023). Methane emission, intake, digestibility, performance and blood metabolites in sheep supplemented with cupuassu and tucuma cake in the eastern Amazon. Frontier in Veterinary Science, 10, 1106619. https://doi.org/10.3389/fvets.2023.1106619
Bugarín-Prado, J. O., Lemus-Flores, C., Grageola-Núñez, F., Valdivia-Bernal, R. y Bonilla-Cárdenas, J. (2021). Efecto de suplementación con harina de aguacate en dietas de corderos sobre crecimiento y el rendimiento de la canal. Abanico Veterinario, 11, 1-12. http://dx.doi.org/10.21929/abavet2021.32
de Evan, T., Carro, M. D., Fernández, Yepes .J. E., Haro, A., Arbesú, L., Romero-Huelva, M. & Molina-Alcaide, E. (2020). Efects of Feeding Multinutrient Blocks Including Avocado Pulp and Peels to Dairy Goats on Feed Intake and Milk Yield and Composition. Animals, 10(2), 194. https://doi.org/10.3390/ani10020194
Díaz, M. T., Álvarez, I., De la Fuente, J., Sañudo, C., Campo, M. M., Oliver, M. A., Font i Furnols, M., Montossi, F., San Julián, R., Nute, G. R. & Cañeque, V. (2005). Fatty acid composition of meat from typical lamb production systems of Spain, United Kingdom, Germany and Uruguay. Meat Science, 71(2), 256–263. https://doi.org/10.1016/j.meatsci.2005.03.020
Folch, J., Lees, M., & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipides from animal tissues. The Journal of biological chemistry, 226(1), 497–509. http://dx.doi.org/10.1016/S0021-9258(18)64849-5
Fránquez, P., Rodríguez, G., Lemus, C., Grageola, F. & Ly, J. (2017). Performance traits and índices of the intake pattern of fattened pigs with fresh paste of whole avocado. Cuban Journal of Agricultural Science, 51(3), 329-336. http://cjascience.com/index.php/CJAS/article/view/755
González-Jiménez, A. C., Mejía-Martínez, K., Becerra-Verdín, E. M., Bugarín-Prado, J. O. & Lemus-Flores. C. (2021). Carcass characteristics and blood biochemical profiles in finishing pigs fed with different levels of avocado paste (Persea americana mill.). Brazilian Journal of Animal and Environmental Research, 4(2), 2344-2359. https://doi.org/10.34188/bjaerv4n2-064
Hernández-López, S. H., Rodríguez-Carpena, J. G., Lemus-Flores, C., Grageola-Nuñez, F. & Estévez, M. (2016). Avocado waste for finishing pigs: Impact on muscle composition andoxidative stability during chilled storage. Meat Science, 116, 186-192. https://doi.org/10.1016/j.meatsci.2016.02.018
Kostik, V., Memeti, S. & Bauer, B. (2013). Fatty acid composition of edible oils and fats. Journal of Hygienic Engineering and Design, 4, 112-116. https://eprints.ugd.edu.mk/11460/1/06. %20Full %20paper %20- %20Vesna %20Kostik %202.pdf
Lemus-Avalos, G., Lemus-Flores, C., Bugarín-Prado, J. O., Grageola-Núñez, F., Ayala-Valdovinos, M. A., Duifhuis-Rivera. T., Moo-Huchin, V. M. & Dzib-Cauich, D. (2020). Effect of diets with avocado meal on lipids in muscle, antioxidants and gene expression in finished pigs. Revista BioCiencias, 7, e968. https://doi.org/10.15741/revbio.07.e968
Lemus-Flores, C., Bugarín-Prado, J. O., Grageola Nuñez, F., Valdivia Bernal, R., Ruiz Dimas, I., Bonilla Cardenas, J. A. & Segura Correa, J. C. (2020). The effect of avocado flour, sunflower oil and different forage: concentrate ratios in the final diet on feed intake, digestibility and productive performance of male sheep. Veterinarski Arhiv, 90(4), 353-364. https://hrcak.srce.hr/file/356220
Ly, J., Fránquez, P., Rodríguez, G., Lemus, C., Domínguez, I. A. & Grageola, F. (2021). Note on in vitro digestion of avocado products for pigs. South African Journal of Animal Science, 51, 138–141. https://hdl.handle.net/10520/ejc-sajas_v51_n1_a16
Mapiye, C., Aalhus, J. L., Turner, T. D., Rolland, D. C., Basarab, J. A., Baron, V. S., McAllister, T. A., Block, H. C., Uttaro, B., Lopez-Campos, O., Proctor, S. D. & Dugan, M. E. R. (2013). Effects of feeding flaxseed or sunflower-seed in high-forage diets on beef production, quality and fatty acid composition. Meat Science, 95, 98-109. https://doi.org/10.1016/j.meatsci.2013.03.033
Miltko, R., Majewska, M. P., Bełżecki, G., Kula, K. & Kowalik, B. (2019). Growth performance, carcass and meat quality of lambs supplemented different vegetable oils. Asian-Australas Journal Animal Science. 32(6), 767-775. https://doi.org/10.5713/ajas.18.0482
Minitab 15 Statistical Software (Minitab v15). (2007). [Computer software]. State College, PA: Minitab, Inc. www.minitab.com
Mohan, N., Harihara, I., Babul, C., Madan, K., Anubrata, D. & Kalita, D. (2012). Effect of dietary sunflower oil and coconut oil on adipose tissue gene expression, fatty acid composition and serum lipid profile of grower pigs. Archives of Animal Nutrition, 66(4), 271-282. https://doi.org/10.1080/1745039X.2012.683324
Rodrigues Cavalcante, I. T., Hauss de Sousa, W., Ribeiro, N. L., Queiroga Cartaxo, F., de Farias Ramos, J. P. & de Azevedo, P. S. (2022). Animal feed based on forage cactus: use of viscera in traditional dishes. Production of by-products that can result in an economic return. Food Science and Technology, 42, e105921. https://doi.org/10.1590/fst.105921
Romero-Bernal, J., Morales Almaraz, E., Castelán Ortega, O. O., Pescador Salas, N. & González-Ronquillo, M. (2017). Chemical composition and fatty acid profile in meat from grazing lamb diets supplemented with ryegrass hay, fishmeal and soya bean meal as PUFA sources. Ciencia Rural Santa María, 47(4), e20160533. http://dx.doi.org/10.1590/0103-8478cr20160533
Ruíz-Dimas, I., Lemus-Flores, C., Bonilla-Cárdenas, J. A., Rodríguez-Carpena, J. G., Valdivia-Bernal, R., Grageola-Núñez, F. & Bugarin-Prado. J. O. (2022). Acceptability of meal of avocado full fruit included in the diet for sheep and pigs. Brazilian Journal of Animal and Environmental Research, Curitiba, 5(1), 193-199. https://doi.org/10.34188/bjaerv5n1-015
Santana, G. y Correa, H. J. (2016). Inclusión de tres fuentes lipídicas en la dieta de vacas lactantes bajo pastoreo sobre el consumo y la digestibilidad. Livestock Research for Rural Development, 28(3), 38. http://www.lrrd.org/lrrd28/3/sant28038.html
Statistical Product and Service Solutions (SPSS). (2011). IBM SPSS Statistics for Windows, Versión 20.0. Armonk, NY: IBM Corp. https://www.ibm.com/mx-es
Steibel, J. P., Poletto, R., Coussens, P. M. & Rosa, G. J. M. (2009). A powerful and flexible linear mixed model framework for the analysis of relative quantification RT-PCR data. Genomics, 94(2), 146-152. https://doi.org/10.1016/j.ygeno.2009.04.008
Wang, X., Yan, S., Shi, B., Zhang, Y. & Guo, X. (2019). Effects of concentrate supplementation on fatty acid composition and expression of lipogenic genes of meat and adipose tissues in grazing lambs. Italian Journal of Animal Science, 18(1), 554-563. https://doi.org/10.1080/1828051X.2018.1547666
Yue, Y., Cheng, X., Zhao, S. G., Liu, Z., Liu, L. S., Zhou, R., Wu, J. P. & Brown, M. A. (2016). Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep. Genet Molecular Research, 15(1), 1-10. http://dx.doi.org/10.4238/gmr.15017323
Zsédely, E., Király, A., Szabó, C. S., Németh, K., Dóka, O. & Schmidt, J. (2012). Effect of dietary linseed oil soap on lamb meat. World Academy Science Engineering and Technology, 6(3), 67-70. https://publications.waset.org/13519/pdf
Araujo, R. G., Rodriguez-Jasso, R. M., Ruiz, H. A., Pintado, M. M. E. & Aguilar, C. N. (2018). Avocado by-products: nutritional and functional properties. Trends in Food Science & Technology, 80, 51-60. https://doi.org/10.1016/j.tifs.2018.07.027
Astuti, D. A., Babab, A. S. & Wibawanc, I. W. T. (2011). Rumen Fermentation, Blood Metabolites, and Performance of Sheep Fed Tropical Browse Plants. Media Peternakan, 34(3), 201-206. https://doi.org/10.5398/medpet.2011.34.3.201
Avellanet, R., Cuenca, R., Pastor, J. y Jordana, J. (2007). Parámetros hematológicos y bioquímico clínicos en la raza ovina xisqueta. Archivos de Zootecnia, 56(Sup. 1), 497-501. https://www.redalyc.org/pdf/495/49509922.pdf
de Castro Budel, J. C., Gomes de Castro, V. C., Mota de Souza, S., de Souza Nahúm, B., Correa Barbosa, A. V., Silva Rodrigues, L., Berndt, A., Navajas Rennó, L., de Morais, E., Rodrigues da Silva, J. A., de Carvalho Rodrigues, T. C. G., Maciel e Silva, A. G. & Lourenço-Junior, J. B. (2023). Methane emission, intake, digestibility, performance and blood metabolites in sheep supplemented with cupuassu and tucuma cake in the eastern Amazon. Frontier in Veterinary Science, 10, 1106619. https://doi.org/10.3389/fvets.2023.1106619
Bugarín-Prado, J. O., Lemus-Flores, C., Grageola-Núñez, F., Valdivia-Bernal, R. y Bonilla-Cárdenas, J. (2021). Efecto de suplementación con harina de aguacate en dietas de corderos sobre crecimiento y el rendimiento de la canal. Abanico Veterinario, 11, 1-12. http://dx.doi.org/10.21929/abavet2021.32
de Evan, T., Carro, M. D., Fernández, Yepes .J. E., Haro, A., Arbesú, L., Romero-Huelva, M. & Molina-Alcaide, E. (2020). Efects of Feeding Multinutrient Blocks Including Avocado Pulp and Peels to Dairy Goats on Feed Intake and Milk Yield and Composition. Animals, 10(2), 194. https://doi.org/10.3390/ani10020194
Díaz, M. T., Álvarez, I., De la Fuente, J., Sañudo, C., Campo, M. M., Oliver, M. A., Font i Furnols, M., Montossi, F., San Julián, R., Nute, G. R. & Cañeque, V. (2005). Fatty acid composition of meat from typical lamb production systems of Spain, United Kingdom, Germany and Uruguay. Meat Science, 71(2), 256–263. https://doi.org/10.1016/j.meatsci.2005.03.020
Folch, J., Lees, M., & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipides from animal tissues. The Journal of biological chemistry, 226(1), 497–509. http://dx.doi.org/10.1016/S0021-9258(18)64849-5
Fránquez, P., Rodríguez, G., Lemus, C., Grageola, F. & Ly, J. (2017). Performance traits and índices of the intake pattern of fattened pigs with fresh paste of whole avocado. Cuban Journal of Agricultural Science, 51(3), 329-336. http://cjascience.com/index.php/CJAS/article/view/755
González-Jiménez, A. C., Mejía-Martínez, K., Becerra-Verdín, E. M., Bugarín-Prado, J. O. & Lemus-Flores. C. (2021). Carcass characteristics and blood biochemical profiles in finishing pigs fed with different levels of avocado paste (Persea americana mill.). Brazilian Journal of Animal and Environmental Research, 4(2), 2344-2359. https://doi.org/10.34188/bjaerv4n2-064
Hernández-López, S. H., Rodríguez-Carpena, J. G., Lemus-Flores, C., Grageola-Nuñez, F. & Estévez, M. (2016). Avocado waste for finishing pigs: Impact on muscle composition andoxidative stability during chilled storage. Meat Science, 116, 186-192. https://doi.org/10.1016/j.meatsci.2016.02.018
Kostik, V., Memeti, S. & Bauer, B. (2013). Fatty acid composition of edible oils and fats. Journal of Hygienic Engineering and Design, 4, 112-116. https://eprints.ugd.edu.mk/11460/1/06. %20Full %20paper %20- %20Vesna %20Kostik %202.pdf
Lemus-Avalos, G., Lemus-Flores, C., Bugarín-Prado, J. O., Grageola-Núñez, F., Ayala-Valdovinos, M. A., Duifhuis-Rivera. T., Moo-Huchin, V. M. & Dzib-Cauich, D. (2020). Effect of diets with avocado meal on lipids in muscle, antioxidants and gene expression in finished pigs. Revista BioCiencias, 7, e968. https://doi.org/10.15741/revbio.07.e968
Lemus-Flores, C., Bugarín-Prado, J. O., Grageola Nuñez, F., Valdivia Bernal, R., Ruiz Dimas, I., Bonilla Cardenas, J. A. & Segura Correa, J. C. (2020). The effect of avocado flour, sunflower oil and different forage: concentrate ratios in the final diet on feed intake, digestibility and productive performance of male sheep. Veterinarski Arhiv, 90(4), 353-364. https://hrcak.srce.hr/file/356220
Ly, J., Fránquez, P., Rodríguez, G., Lemus, C., Domínguez, I. A. & Grageola, F. (2021). Note on in vitro digestion of avocado products for pigs. South African Journal of Animal Science, 51, 138–141. https://hdl.handle.net/10520/ejc-sajas_v51_n1_a16
Mapiye, C., Aalhus, J. L., Turner, T. D., Rolland, D. C., Basarab, J. A., Baron, V. S., McAllister, T. A., Block, H. C., Uttaro, B., Lopez-Campos, O., Proctor, S. D. & Dugan, M. E. R. (2013). Effects of feeding flaxseed or sunflower-seed in high-forage diets on beef production, quality and fatty acid composition. Meat Science, 95, 98-109. https://doi.org/10.1016/j.meatsci.2013.03.033
Miltko, R., Majewska, M. P., Bełżecki, G., Kula, K. & Kowalik, B. (2019). Growth performance, carcass and meat quality of lambs supplemented different vegetable oils. Asian-Australas Journal Animal Science. 32(6), 767-775. https://doi.org/10.5713/ajas.18.0482
Minitab 15 Statistical Software (Minitab v15). (2007). [Computer software]. State College, PA: Minitab, Inc. www.minitab.com
Mohan, N., Harihara, I., Babul, C., Madan, K., Anubrata, D. & Kalita, D. (2012). Effect of dietary sunflower oil and coconut oil on adipose tissue gene expression, fatty acid composition and serum lipid profile of grower pigs. Archives of Animal Nutrition, 66(4), 271-282. https://doi.org/10.1080/1745039X.2012.683324
Rodrigues Cavalcante, I. T., Hauss de Sousa, W., Ribeiro, N. L., Queiroga Cartaxo, F., de Farias Ramos, J. P. & de Azevedo, P. S. (2022). Animal feed based on forage cactus: use of viscera in traditional dishes. Production of by-products that can result in an economic return. Food Science and Technology, 42, e105921. https://doi.org/10.1590/fst.105921
Romero-Bernal, J., Morales Almaraz, E., Castelán Ortega, O. O., Pescador Salas, N. & González-Ronquillo, M. (2017). Chemical composition and fatty acid profile in meat from grazing lamb diets supplemented with ryegrass hay, fishmeal and soya bean meal as PUFA sources. Ciencia Rural Santa María, 47(4), e20160533. http://dx.doi.org/10.1590/0103-8478cr20160533
Ruíz-Dimas, I., Lemus-Flores, C., Bonilla-Cárdenas, J. A., Rodríguez-Carpena, J. G., Valdivia-Bernal, R., Grageola-Núñez, F. & Bugarin-Prado. J. O. (2022). Acceptability of meal of avocado full fruit included in the diet for sheep and pigs. Brazilian Journal of Animal and Environmental Research, Curitiba, 5(1), 193-199. https://doi.org/10.34188/bjaerv5n1-015
Santana, G. y Correa, H. J. (2016). Inclusión de tres fuentes lipídicas en la dieta de vacas lactantes bajo pastoreo sobre el consumo y la digestibilidad. Livestock Research for Rural Development, 28(3), 38. http://www.lrrd.org/lrrd28/3/sant28038.html
Statistical Product and Service Solutions (SPSS). (2011). IBM SPSS Statistics for Windows, Versión 20.0. Armonk, NY: IBM Corp. https://www.ibm.com/mx-es
Steibel, J. P., Poletto, R., Coussens, P. M. & Rosa, G. J. M. (2009). A powerful and flexible linear mixed model framework for the analysis of relative quantification RT-PCR data. Genomics, 94(2), 146-152. https://doi.org/10.1016/j.ygeno.2009.04.008
Wang, X., Yan, S., Shi, B., Zhang, Y. & Guo, X. (2019). Effects of concentrate supplementation on fatty acid composition and expression of lipogenic genes of meat and adipose tissues in grazing lambs. Italian Journal of Animal Science, 18(1), 554-563. https://doi.org/10.1080/1828051X.2018.1547666
Yue, Y., Cheng, X., Zhao, S. G., Liu, Z., Liu, L. S., Zhou, R., Wu, J. P. & Brown, M. A. (2016). Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep. Genet Molecular Research, 15(1), 1-10. http://dx.doi.org/10.4238/gmr.15017323
Zsédely, E., Király, A., Szabó, C. S., Németh, K., Dóka, O. & Schmidt, J. (2012). Effect of dietary linseed oil soap on lamb meat. World Academy Science Engineering and Technology, 6(3), 67-70. https://publications.waset.org/13519/pdf
Publicado
2024-02-04
Como Citar
Lemus , C., Bugarín, J., Lemus , G., Rodríguez , J., Peña, G., & Loya, J. (2024). Associação da expressão gênica com metabólitos sanguíneos e perfil de ácidos graxos em cordeiros. Revista Da Faculdade De Agronomia Da Universidade De Zulia, 41(1), e244102. Obtido de https://produccioncientificaluz.org/index.php/agronomia/article/view/41580
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Direitos de Autor (c) 2024 Clemente Lemus Flores, Job Oswaldo Bugarín Prado, Gilberto Lemus Avalos, Javier Germán Rodríguez Carpena, Gabriela Rosario Peña Sandoval, José Lenin Loya Olguin
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