Avaliação do teor de clorofila e da estabilidade da membrana sob estresse oxidativo induzido pelo herbicida glifosato como indicadores de tolerância à seca em algumas linhagens avançadas de trigo duro (Triticum durum L.): estudo in vitro

• Sarah Benkadja Department of Agronomy, VRBN Laboratory, Faculty of Nature and Life Sciences, Farhat Abbas University of Setif, Algeria. https://orcid.org/0000-0001-7737-6135
• Abdelmalek Oulmi Department of Biology and Plant Ecology, VRBN Laboratory, Faculty of Nature and Life Sciences, Farhat Abbas University of Setif, Algeria. https://orcid.org/0000-0002-1716-711X
• Benalia Frih Department of Biology and Plant Ecology, VRBN Laboratory, Faculty of Nature and Life Sciences, Farhat Abbas University of Setif, Algeria. https://orcid.org/0000-0001-6463-7340
• Hocine Bendada Department of Agricultural Sciences, Relizane University, Relizane, Algeria. https://orcid.org/0000-0002-8997-5933
• Ali Guendouz National Agronomic Research Institute of Algeria (INRAA), Setif Research Unit, Algeria. https://orcid.org/0000-0002-9081-6497
• Amar Benmahammed Department of Biology and Plant Ecology, VRBN Laboratory, Faculty of Nature and Life Sciences, Farhat Abbas University of Setif, Algeria. https://orcid.org/0000-0001-7300-0245

Palavras-chave: vazamento de eletrólitos, perda de clorofila, tolerante, sensibilidade

Resumo

Estresse oxidativo causado pelo glifosato é um fenômeno químico e fisiológico complexo e se desenvolve como resultado da superprodução e acúmulo de espécies reativas de oxigênio (ROS). Este estudo foi realizado in vitro no Instituto Nacional de Pesquisas Agronômicas da Argélia (INRAA) Setif, para selecionar o trigo duro mais suscetível (Triticum durum L.) sob estresse oxidativo induzido pelo herbicida glifosato, avaliando a degradação do teor de clorofila e o vazamento da membrana celular. Os genótipos mostraram variações significativas em quase todos os traços estudados. A taxa de perda de clorofila variou de 26,42 % para o genótipo G5 a 48,75 % para a variedade local Boutaleb, os valores do índice de sensibilidade ao glifosato foram encontrados entre 0,65-1,2, a linha avançada G5 foi encontrada como a mais tolerante sob estresse oxidativo com a menor taxa de perda de clorofila e o menor índice de sensibilidade ao glifosato. Além disso, a linha avançada G4 registrou o maior vazamento de eletrólitos (80,16%), enquanto G6 apresentou a estimativa mais baixa (50,77 %). Portanto, as linhas avançadas G5 e G6 parecem ser as mais adequadas para as condições de crescimento.

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