Identificação das bactérias intestinais da traça-cera
Palavras-chave:
Galleria mellonella, plástico, sequência de DNA, microrganismos, Pseudomonas
Resumo
Globalmente, o uso de polímeros industriais de fontes de combustíveis fósseis é praticamente inevitável devido à diversidade de aplicações; porém, os problemas ambientais e ambientais que estão levando à busca de alternativas que permitam reduzir o uso destes, bem como estratégias para controlá-los por meio da degradação, com a participação de alguns agentes ativos bioorgânicos. Os insetos são de interesse, como possíveis biodegradantes devido à ação de bactérias no trato digestivo. Levando em consideração esses antecedentes, este estudo teve como objetivo identificar as bactérias presentes no intestino das larvas da traça-cera (Galleria mellonella). Trinta larvas foram alimentadas com dieta de isopor y trinta larvas em dieta natural por um período de 7 dias. Posteriormente, as larvas foram retiradas para estudo do trato gastrointestinal por PCR. Resultados obtidos mostraram a presencia de células bacterianas de Carnobacterium maltaromaticum, Brevibacterium sandarakinum, Pseudomonas psychrophila, Pseudomonas sp., Providencia sp., Corynebacterium sp. Porém, é necessário verificar a real ação isolada desses grupos de bactérias na degradação efetiva dos polímeros.
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Referências
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Huerta, L., Wanga, E., Thapa, B., Yang, X., Gertsen, H., Salánki, T., Geissen, V. & Garbeva, P. (2018). Decay of low-density polyethylene by bacteria extracted from earthworm's guts: A potential for soil restoration. Science of The Total Environment, 624, 753-757. doi: https://doi.org/10.1016/j.scitotenv.2017.12.144
Kämpfer, P., Schäfer, J., Lodders, N. & Busse, H.J. (2010). Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment. International Journal of Systematic and Evolutionary Microbiology, 60, 909-913. doi: 10.1099/ijs.0.014100-0
Kim, H.R., Lee, H.M., Yu, H.C., Jeon, E., Lee, S., Li, J. & Kim, D.H. (2020). Biodegradation of polystyrene by Pseudomonas sp. isolated from the gut of superworms (Larvae of Zophobas atratus). Environmental Science & Technology, 54, (11):6987-6996. doi: 10.1021/acs.est.0c01495.
Kong, H. G., Kim, H. H., Chung, J. H., Jun, J. H., Lee, S., Kim, H. M., et al. (2019). The Galleria mellonella hologenome supports microbiota-independent metabolism of long-chain hydrocarbon beeswax. Cell Reports, 26, 2451–2464. doi: https://doi.org/10.1016/j.celrep.2019.02.018.
Kumar, S. & Raut, S. (2015). Microbial degradation of low density polyethylene (LDPE): A review. Journal of Environmental Chemical Engineering, 3(1), 462-473. doi: https://doi.org/10.1016/j.jece.2015.01.003
Leisner, J.J., Laursen, B.G., Djamel, H.P. & Dalgaard, P. (2007). Carnobacterium: positive and negative ejects in the environment and in foods. FEMS Microbiology Reviews, 31, 592-613. doi: 10.1111/j.1574-6976.2007.00080.x
Lewin, G.R., Marc, C.C., Horn, H.A., Mcdonald, B.R., Stankey, R.J., Fox, B.G. & Currie, C.R. (2016). Evolution and ecology of Actinobacteria and their bioenergy applications. Annual Review of Microbiology, 70, 235-254. doi: 10.1146/annurev-micro-102215-095748.
Lou, Y., Ekaterina, P., Yang S.S., Lu, B., Liu, B., Ren, N., Corvini, P., & Xing, D. (2020). Biodegradation of polyethylene and polystyrene by greater wax moth larvae (Galleria mellonella L.) and the effect of co-diet supplementation on the core gut microbiome. Environmental Science & Technology, 54, 2821-2831. doi:https://dx.doi.org/10.1021/acs.est.9b07044
Mason, C.J., Clair, A., Peiffer, M., Gomez, E., Jones, A.G., Felton, G.W. & Hoover, K. (2020). Diet influences proliferation and stability of gut bacterial populations in herbivorous lepidopteran larvae. PLoS ONE, 15(3), e0229848. doi: 10.1371/journal.pone.0229848
Mukherjee, K., Raju, R., Fischer, R. & Vilcinskas, A. (2013). Galleria mellonella as a model host to study gut microbe homeostasis and brain infection by the human pathogen Listeria monocytogenes. Advances in Biochemical Engineering / Biotechnology, 135, 27-39. doi: 10.1007/10_2013_203
Ng, E., Huerta, E., Eldridge, S., Johnston, P., Hu, H., Geissen, V. & Chen, D. (2018). An overview of microplastic and nanoplastic pollution in agroecosystems. Science of The Total Environment, 627, 1377-1388. doi: https://doi.org/10.1016/j.scitotenv.2018.01.341
Ren, L., Men, L., Zhang, Z., Guan, F., Tian, J., Wang, B., Wang, J., Zhang, Y. & Zhang W. (2019). Biodegradation of polyethylene by Enterobacter sp. D1 from the guts of wax moth Galleria mellonella. International Journal of Environmental Research and Public Health, 16, 1941. doi:10.3390/ijerph16111941.
Rizzi, A., Crotti, E., Borruso, L., Jucker, C., Lupi, D., Colombo, M. & Daffonchio, D. (2013). Characterization of the bacterial community associated with larvae and adults of Anoplophora chinensis collected in Italy by culture and culture-independent methods. BioMed Research International, 2013, 420287. doi: 10.1155/2013/420287
Ruiz, J., Vilanova-Cuevas, B., Alvarez, A., Martín, E., Malizia, A., Galindo-Cardona, A., de Cristóbal, R., Occhionero, M., Chalup, A., Monmany-Garzía, A. & Godoy-Vitorino, F. (2022). The bacterial and fungal gut microbiota of the greater wax moth, Galleria mellonella L. consuming polyethylene and polystyrene. Frontiers in Microbiology, 13, 918861. 861. doi: 10.3389/fmicb.2022.918861
Santo, M., Weitsman, R. & Silvan, A. (2013). The role of the copper-binding enzyme – laccase - in the biodegradation of polyethylene by the actinomycete Rhodococcus ruber. International Biodeterioration & Biodegradation, 84; 204-210. https://doi.org/10.1016/j.ibiod.2012.03.001
Shannon, A.L., Attwood, G., Hopcroft, D.H. & Christeller,J.T. (2001). Characterization of lactic acid bacteria in the larval midgut of the keratinophagous lepidopteran, Hofmannophila pseudospretella. Letters in Applied Microbiology, 32, 36-41. doi: 10.1046/j.1472-765x.2001.00854.x
Silva, A.B., Bastos, A.S., Justino, C., Da Costa, J., Duarte, A. & Rocha-Santos, T. (2018). Microplastics in the environment: Challenges in analytical chemistry, A review. Analytica Chimica Acta, 1017; 1-19. doi: https://doi.org/10.1016/j.aca.2018.02.043
Suzuki, M., Taylor, L. & Delong, E. (2000). Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-Nuclease assays. Applied and Environmental Microbiology, 66(11), 4605-4614. doi: https://doi.org/10.1128/AEM.66.11.4605-4614.2000
Torres De La Cruz, M., Cortez, H., Ortiz, C., Cappello, S. y Pérez De La Cruz, M. (2014). Cepas monospóricas de Metarhizium anisopliae y su patogenicidad sobre Galleria mellonella en Tabasco, México. Revista Mexicana de Ciencias Pecuarias, 5(2), 171-180. https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242014000200004
Wickramasinghe, N.N., Ravensdale, J., Coorey, R., Chandry, S.P. & Dykes, G.A. (2019). The predominance of psychrotrophic Pseudomonads on aerobically stored chilled red meat. Comprehensive Reviews in Food Science and Food Safety, 18; 1622-1635. doi: https://doi.org/10.1111/1541-4337.12483
Wilkes, R.A. & Aristilde, L. (2017). Degradation and metabolism of synthetic plastics and associated products by Pseudomonas sp.: capabilities and challenges. Journal of Applied Microbiology, 123(3); 582-593. doi: 10.1111/jam.13472
Yang, S.S., Brandon, A., Flanagan, J., Yang, J., Ning, D., Cai, S., Fan, H., Wang, Z., Ren, J., Benbow, E., Ren, N., Wamouth, R., Zhou, J., Criddle, C. & Wu, W. (2017). Biodegradation of de polystyrene wastes in yellow mealworms (larvae of Tenebrio molitor L.): Factors affecting biodegradation rates and the ability of polystyrene-fed larvae to complete their life cycle. Chemosphere, 191, 979-989. doi: 10.1016/j.chemosphere.2017.10.117
Yang, Y., Yang, J., Wu, W., Zhao, J., Song, Y., Gao, L., Yang, R. & Jiang, L. (2015). Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 2. Role of gut microorganisms. Environmental Science and Technology, 49(20), 12087-12093. doi: https://doi.org/10.1021/acs.est.5b02663
Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., Toyohara, K., Miyamoto, K., Kimura, Y. & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199. doi: 10.1126/science.aad6359
Agudelo-Londoño, N., Torres-Taborda, M.M., Alvarez-López, C. y Vélez-Acosta, L.M. (2015). Bacteriocinas producidas por bacterias ácido lácticas y su aplicación en la industria de alimentos. Revista Alimentos Hoy, 23, 186-205. https://alimentoshoy.acta.org.co/index.php/hoy/article/viewFile/356/306
Brandon, A.M., Gao, S.H., Tian, R., Ning, D., Yang, S.S., Zhou, J., Wu, W.M. & Criddle, C.S. (2018). Biodegradation of polyethylene and plastic mixtures in mealworms (larvae of Tenebrio molitor) and effects on the gut microbiome. Environmental Science & Technology, 52, 6526-6533. doi: https://doi.org/10.1021/acs.est.8b02301
Bombelli, P., Howe, J. & Bertocchini, C. (2017). Polyethylene bio-degradation by caterpillars of the wax moth Galleria mellonella. Current Biology, 27(8), 292-293. doi: https://doi.org/10.1016/j.cub.2017.02.060
Frías, C., Ize, I. & Gavilán, A. (2003). La situación de los envases de plástico en México. Gaceta Ecológica, 69, 67-82. https://www.redalyc.org/pdf/539/53906905.pdf
Geyer, R., Jambeck, J. & Lavender, K. (2017). Production, use, and fate of all plastics ever made. Science Advances, 3(7), e1700782. DOI: 10.1126/sciadv.1700782
Ghatge, S., Yang, Y., Ahn, J.H. & Hur, H.G. (2020). Biodegradation of polyethylene: a brief review. Applied Biological Chemistry, 63, 27. doi: https://doi.org/10.1186/s13765-020-00511-3.
Hadad, D., Geresh, S. & Sivan, A. (2005). Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis. Journal of Applied Microbiology, 98, 1093-1100. doi: 10.1111/j.1365-2672.2005.02553.x
Huerta, L., Wanga, E., Gertsen, H., Gooren, H., Peters, P., Salanki, T., Van Der Ploeg, M., Besseling, E., Koelmans, A. & Geissen, V. (2016). Microplastics in the terrestrial ecosystem: implications for Lumbricus terrestres (Oligochaeta, Lumbricidae). Environmental Science & Technology, 50, 2685-2691. doi: https://doi.org/10.1021/acs.est.5b05478
Huerta, L., Wanga, E., Thapa, B., Yang, X., Gertsen, H., Salánki, T., Geissen, V. & Garbeva, P. (2018). Decay of low-density polyethylene by bacteria extracted from earthworm's guts: A potential for soil restoration. Science of The Total Environment, 624, 753-757. doi: https://doi.org/10.1016/j.scitotenv.2017.12.144
Kämpfer, P., Schäfer, J., Lodders, N. & Busse, H.J. (2010). Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment. International Journal of Systematic and Evolutionary Microbiology, 60, 909-913. doi: 10.1099/ijs.0.014100-0
Kim, H.R., Lee, H.M., Yu, H.C., Jeon, E., Lee, S., Li, J. & Kim, D.H. (2020). Biodegradation of polystyrene by Pseudomonas sp. isolated from the gut of superworms (Larvae of Zophobas atratus). Environmental Science & Technology, 54, (11):6987-6996. doi: 10.1021/acs.est.0c01495.
Kong, H. G., Kim, H. H., Chung, J. H., Jun, J. H., Lee, S., Kim, H. M., et al. (2019). The Galleria mellonella hologenome supports microbiota-independent metabolism of long-chain hydrocarbon beeswax. Cell Reports, 26, 2451–2464. doi: https://doi.org/10.1016/j.celrep.2019.02.018.
Kumar, S. & Raut, S. (2015). Microbial degradation of low density polyethylene (LDPE): A review. Journal of Environmental Chemical Engineering, 3(1), 462-473. doi: https://doi.org/10.1016/j.jece.2015.01.003
Leisner, J.J., Laursen, B.G., Djamel, H.P. & Dalgaard, P. (2007). Carnobacterium: positive and negative ejects in the environment and in foods. FEMS Microbiology Reviews, 31, 592-613. doi: 10.1111/j.1574-6976.2007.00080.x
Lewin, G.R., Marc, C.C., Horn, H.A., Mcdonald, B.R., Stankey, R.J., Fox, B.G. & Currie, C.R. (2016). Evolution and ecology of Actinobacteria and their bioenergy applications. Annual Review of Microbiology, 70, 235-254. doi: 10.1146/annurev-micro-102215-095748.
Lou, Y., Ekaterina, P., Yang S.S., Lu, B., Liu, B., Ren, N., Corvini, P., & Xing, D. (2020). Biodegradation of polyethylene and polystyrene by greater wax moth larvae (Galleria mellonella L.) and the effect of co-diet supplementation on the core gut microbiome. Environmental Science & Technology, 54, 2821-2831. doi:https://dx.doi.org/10.1021/acs.est.9b07044
Mason, C.J., Clair, A., Peiffer, M., Gomez, E., Jones, A.G., Felton, G.W. & Hoover, K. (2020). Diet influences proliferation and stability of gut bacterial populations in herbivorous lepidopteran larvae. PLoS ONE, 15(3), e0229848. doi: 10.1371/journal.pone.0229848
Mukherjee, K., Raju, R., Fischer, R. & Vilcinskas, A. (2013). Galleria mellonella as a model host to study gut microbe homeostasis and brain infection by the human pathogen Listeria monocytogenes. Advances in Biochemical Engineering / Biotechnology, 135, 27-39. doi: 10.1007/10_2013_203
Ng, E., Huerta, E., Eldridge, S., Johnston, P., Hu, H., Geissen, V. & Chen, D. (2018). An overview of microplastic and nanoplastic pollution in agroecosystems. Science of The Total Environment, 627, 1377-1388. doi: https://doi.org/10.1016/j.scitotenv.2018.01.341
Ren, L., Men, L., Zhang, Z., Guan, F., Tian, J., Wang, B., Wang, J., Zhang, Y. & Zhang W. (2019). Biodegradation of polyethylene by Enterobacter sp. D1 from the guts of wax moth Galleria mellonella. International Journal of Environmental Research and Public Health, 16, 1941. doi:10.3390/ijerph16111941.
Rizzi, A., Crotti, E., Borruso, L., Jucker, C., Lupi, D., Colombo, M. & Daffonchio, D. (2013). Characterization of the bacterial community associated with larvae and adults of Anoplophora chinensis collected in Italy by culture and culture-independent methods. BioMed Research International, 2013, 420287. doi: 10.1155/2013/420287
Ruiz, J., Vilanova-Cuevas, B., Alvarez, A., Martín, E., Malizia, A., Galindo-Cardona, A., de Cristóbal, R., Occhionero, M., Chalup, A., Monmany-Garzía, A. & Godoy-Vitorino, F. (2022). The bacterial and fungal gut microbiota of the greater wax moth, Galleria mellonella L. consuming polyethylene and polystyrene. Frontiers in Microbiology, 13, 918861. 861. doi: 10.3389/fmicb.2022.918861
Santo, M., Weitsman, R. & Silvan, A. (2013). The role of the copper-binding enzyme – laccase - in the biodegradation of polyethylene by the actinomycete Rhodococcus ruber. International Biodeterioration & Biodegradation, 84; 204-210. https://doi.org/10.1016/j.ibiod.2012.03.001
Shannon, A.L., Attwood, G., Hopcroft, D.H. & Christeller,J.T. (2001). Characterization of lactic acid bacteria in the larval midgut of the keratinophagous lepidopteran, Hofmannophila pseudospretella. Letters in Applied Microbiology, 32, 36-41. doi: 10.1046/j.1472-765x.2001.00854.x
Silva, A.B., Bastos, A.S., Justino, C., Da Costa, J., Duarte, A. & Rocha-Santos, T. (2018). Microplastics in the environment: Challenges in analytical chemistry, A review. Analytica Chimica Acta, 1017; 1-19. doi: https://doi.org/10.1016/j.aca.2018.02.043
Suzuki, M., Taylor, L. & Delong, E. (2000). Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-Nuclease assays. Applied and Environmental Microbiology, 66(11), 4605-4614. doi: https://doi.org/10.1128/AEM.66.11.4605-4614.2000
Torres De La Cruz, M., Cortez, H., Ortiz, C., Cappello, S. y Pérez De La Cruz, M. (2014). Cepas monospóricas de Metarhizium anisopliae y su patogenicidad sobre Galleria mellonella en Tabasco, México. Revista Mexicana de Ciencias Pecuarias, 5(2), 171-180. https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242014000200004
Wickramasinghe, N.N., Ravensdale, J., Coorey, R., Chandry, S.P. & Dykes, G.A. (2019). The predominance of psychrotrophic Pseudomonads on aerobically stored chilled red meat. Comprehensive Reviews in Food Science and Food Safety, 18; 1622-1635. doi: https://doi.org/10.1111/1541-4337.12483
Wilkes, R.A. & Aristilde, L. (2017). Degradation and metabolism of synthetic plastics and associated products by Pseudomonas sp.: capabilities and challenges. Journal of Applied Microbiology, 123(3); 582-593. doi: 10.1111/jam.13472
Yang, S.S., Brandon, A., Flanagan, J., Yang, J., Ning, D., Cai, S., Fan, H., Wang, Z., Ren, J., Benbow, E., Ren, N., Wamouth, R., Zhou, J., Criddle, C. & Wu, W. (2017). Biodegradation of de polystyrene wastes in yellow mealworms (larvae of Tenebrio molitor L.): Factors affecting biodegradation rates and the ability of polystyrene-fed larvae to complete their life cycle. Chemosphere, 191, 979-989. doi: 10.1016/j.chemosphere.2017.10.117
Yang, Y., Yang, J., Wu, W., Zhao, J., Song, Y., Gao, L., Yang, R. & Jiang, L. (2015). Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 2. Role of gut microorganisms. Environmental Science and Technology, 49(20), 12087-12093. doi: https://doi.org/10.1021/acs.est.5b02663
Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., Toyohara, K., Miyamoto, K., Kimura, Y. & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199. doi: 10.1126/science.aad6359
Publicado
2022-12-26
Como Citar
Betancourt, O., Araneda, X., Pesenti, H., & Anabalón, L. (2022). Identificação das bactérias intestinais da traça-cera. Revista Da Faculdade De Agronomia Da Universidade De Zulia, 40(1), e234005. Obtido de https://produccioncientificaluz.org/index.php/agronomia/article/view/39417
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Produção Vegetal
Direitos de Autor (c) Oriana Lisette Betancourt Gallegos, Ximena Andrea Araneda Duran. Héctor Gonzalo Pesenti Pérez, Leonardo Iván Anabalón Rodríguez
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