Mutagênese induzida em grama de janeiro por etil-meta-sulfonato e seu efeito no controle da cigarrinha
Palavras-chave:
Melhoramento genético, variabilidade, melhoramento, mutações
Resumo
Nesta pesquisa, a dose letal mediana (DL50) e a resistência à cigarrinha (Mahanarva andigena) foram determinadas, utilizando-se um agente mutagenizante etil meta-sulfonato (EMS) em capim-janeiro (Eriochloa polystachya Kunth) como meio de gerar mutações. O estudo foi realizado em nível de laboratório e casa de vegetação, utilizando um Desenho Aleatório Completo (DCA) com dez tratamentos e três repetições, que consistiu em 5 doses de EMS (0,00 %, 0,25 %, 0,50 %, 0,75 % e 1,00 %) por 2 tempos de impregnação (24 e 48 horas). De acordo com os resultados, as doses de etil meta-sulfonato (EMS) tiveram efeito na fixação dos estolões do capim-janeiro, apresentando alto índice de deflação em doses superiores a 0,50%. Os tratamentos com doses de 0,25% EMS às 48 horas obtiveram o melhor desempenho agronômico até 95 dias, onde a altura das plantas teve seu melhor desenvolvimento com uso próximo ao determinado como LD50 (0,32%). O capim-janeiro impregnado com EMS apresentou danos leves causados pelo salivazo.
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Referências
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Bhattarai, G., Schmid, R. & McCornack, B. (2019). Remote sensing data to detect Hessian fly infestation in commercial wheat fields. Scientific Reports, 9, 6109. https://doi.org/10.1038/s41598-019-42620-0.
Castro, U., Morales, A. & Peck, D. (2005). Population dynamics and phenology of spittlebugs from Zulia carbonaria (Lallemand) pastures (Homoptera: Cercopidae) in the geographic valley of the Cauca River, Colombia. Neotropical Entomology, 34(3), 459-470. https://doi.org/10.1590/S1519-566X2005000300015
Corrales-Lerma, R., Avendaño-Arrazate, C., Morales-Nieto, C.,
Santellano-Estrada, E., Villarreal-Guerrero, F., Melgoza-Castillo, A.,
Álvarez-Holguín, A. & Gómez-Simuta, Y. (2019).
Radiación gamma para inducción de mutagénesis en pasto rosado
(Melinis repens (Willd.) Zizka). Acta Universitaria, 29, 1-10. https://doi.org/10.15174/au.2019.1847.
Cobos, F., Gómez, L., Reyes Borja, W., Hasang, E., Ruilova, M. & Duran, P. (2021). Effects of salinity levels in Oryza sativa in different phenological stages under greenhouse conditions. Revista de la Facultad de Agronomía de la Universidad del Zulia, 39(1), e223905. https://doi.org/10.47280/RevFacAgron(LUZ).v39.n1.05.
Costa, A. (2017). Indución y evaluación de mutantes de alcachofa tolerantes a salinidad. Universitas Miguel Hernández. http:// http://dspace.umh.es/bitstream/11000/3445/1/Costa%20Ortiz%2C%20Aida%20TFGBiotec%202014-15.pdf.
Emrani, S., Arzani, A. & Saeidi, G. (2011). Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens. African Journal of Biotechnology, 10(59), 12602-12613. https://doi.org/10.5897/AJB11.329.
Gómez, J., Aguirre, L., Gómez, L., Reyes, W., Rodríguez, J. & Arana, L. (2020). Dosis letal media para inducir mutaciones, con rayos gamma, en pastojaneiro (Eriochloa polystachya Kunth). Revista de Producción Animal, 32(1), 73-83. https://revistas.reduc.edu.cu/index.php/rpa/article/view/e339.
González, M. (2014). Desarrollo de una plataforma de tilling en melón (Cucumis melo L.). Tesis Doctoral. Universitat Autónoma de Barcelona, Barcelona, España. 282 p. https://ddd.uab.cat/record/127025.
Greene, E., Codomo, C., Taylor, N., Henikoff, J., Till, B., Reynolds, S., Enns L., Burtner C., Johnson, J., Odden, A. & Steven, L. (2003). Spectrum of Chemically induced mutations from large-scale reverse genetic screening in Arabidopsis. Genetics, 164(2), 731–740. https://doi.org/10.1093/genetics/164.2.731.
Hasang, E., Gómez, J., Moreira, E. & Cobos, F. (2020). Variabilidad fenotípica, y desarrollo de estolones de pasto Janeiro (Eriochloa polystachya Kunth), irradiados a 52 gy de rayos gamma (60co). Journal of Science and Research, 5, 96-109. https://revistas.utb.edu.ec/index.php/sr/article/view/1000.
Khalil, F., Naiyan, X., Tayyab, M. & Pinghua, C. (2018). Screening of EMS-Induced Drought-Tolerant Sugarcane Mutants Employing Physiological, Molecular and Enzymatic Approaches. Agronomy, 8(10), 226. doi:https://doi.org/10.3390/agronomy8100226.
Konzak, C., Nilan, R., Wagner, T. & Foster, R. (1965). Efficient chemical mutagenesis, the use of induced mutations in Plant Breeding (Rep. FAO/IAEA Tech. Meeting Rome, 1964). Porgamon Press, Oxford. p. 49–70.
Kumar, G. & Pandey, A. (2020). Ethyl metha- sulfonate-induced changes in cyto-morphological and biochemical aspects of Coriandrum sativum L. Journal of the Saudi Society of Agricultural Sciences, 18(4), 469-475. https://doi.org/10.1016/j.jssas.2018.03.003.
López, E. (2011). Inducción de variabilidad genética para tolerancia a estreses abióticos mediante técnicas de cultivo in vitro en Cenchrus ciliaris L. Universidad Internacional de Andalucía, España. 113 p. https://core.ac.uk/download/pdf/72021262.pdf.
Mendoza, R. (2020). Inducción mutagénica en cultivares de Eustoma grandiflorum mediante el uso de metanosulfonato de etilo (EMS). Centro de Investigación y Asistencia en Tecnología y
diseño del estado de Jalisco, A.C, México. https://ciatej.repositorioinstitucional.mx/jspui/bitstream/1023/746/1/Rafael%20Mendoza%20G%C3%B3mez%20Bio.pdf.
Mostafa, G. (2015). Effect of some chemical mutagens on growth, phytochemical composition and the induction of mutations in Khaya senegalensis. International Journal of Plant Breeding and Genetics, 9(2), 57-67. https://doi:10.3923 / ijpbg.2015.57.67.
Nobel, P. (1999). Physicochemical & Environmental. Plant Physiology. Second Edition. Academic Press. San Diego, California, USA. 474 p. https://books.google.co.ve/books?id=yW_pptyiMHoC&dq=Nobel,+P.+(1999).&lr=&hl=es&source=gbs_navlinks_s.
Pankhurst, C, Blair, M & Broughton, W. (2004). Tilling the beans. In Proceedings of the 3rd International Scientific Meeting, Phaseomics III, pp. 13-15.
Porch, T., Blair, M., Lariguet, P., Galeano, C., Pankhurst, C. & Broughton, W. (2009). Generation of a Mutant Population for TILLING Common Bean Genotype BAT 93. Journal of the American Society for Horticultural Science, 134(3), 348-355. https://doi.org/10.21273/JASHS.134.3.348.
Rodríguez, N., Torres, C., Chaman, M. & Hidalgo J. (2019). Efecto del estrés salino en el crecimiento y contenido relativo del agua en las variedades IR-43 y amazonas de Oryza sativa "arroz" (Poaceae). Arnaldoa, 26(3), 931-942. https://dx.doi.org/10.22497/arnaldoa.263.26305.
Rojas, L., Collado, R., León, A., Rivero, L., Ocaña, B., Hernández, M., Veitía, N., Martirena, A., Torres, D. & García, L. (2016). Concentración óptima de metano sulfonato de etilo en Phaseolus vulgaris L. cv. ‘DOR 364’ para inducir variaciones fenotípicas. Biotecnología Vegetal, 16(3), 179-188. https://revista.ibp.co.cu/index.php/BV/article/view/526.
Sharma, S., Sood, R & Pandey, D. (2005). Studies on mutagen sensitivity, effectiveness and efficiency in urdbean [Vigna mungo (L.) Hepper]. Indian Journal of Genetics and Plant Breeding, 65(1), 20–22. https://www.isgpb.org/journal/index.php/IJGPB/article/view/1602.
Sotelo, G., Cardona, C & Miles, J. (2003). Desarrollo de híbridos de Brachiaria resistentes a cuatro especies de salivazo (Homoptera: Cercopidae). Revista Colombiana de Entomología, 29 (2): 157-163. https://doi.org/10.25100/socolen.v29i2.9599.
Suthakar, V. & Mullainathan, L. (2015). Studies on Effect of Physical and Chemical Mutagens in Sorghum (Sorghum bicolor (L.) Moench) in M2 Generation". International Letters of Natural Sciences, 37, 46-50. https://doi.org/10.18052/www.scipress.com/ILNS.37.46.
Yadav, P., Meena, H. S., Meena, P. D., Kumar, A., Gupta, R., Jambhulkar, S. & Singh, D. (2016). Determination of LD50 of ethyl methanesulfonate (EMS) for induction of mutations in rapeseed-mustard. Journal of Oilseed Brassica, 1(1), 77-82. http://srmr.org.in/ojs/index.php/job/article/download/33/32.
Ashok, V., Kumari, R., Amutha, R., Siva, T., Juliet, S. & Ananda, C. (2009). Effect of chemical mutagen on expression of characters in arid legume pulse–cowpea (Vigna unguiculata (L.) Walp.). Research Journal of Agriculture and Biological Sciences, 5(6), 1115–1120. http://www.aensiweb.net/AENSIWEB/rjabs/rjabs/2009/1115-1120.pdf
Bhattarai, G., Schmid, R. & McCornack, B. (2019). Remote sensing data to detect Hessian fly infestation in commercial wheat fields. Scientific Reports, 9, 6109. https://doi.org/10.1038/s41598-019-42620-0.
Castro, U., Morales, A. & Peck, D. (2005). Population dynamics and phenology of spittlebugs from Zulia carbonaria (Lallemand) pastures (Homoptera: Cercopidae) in the geographic valley of the Cauca River, Colombia. Neotropical Entomology, 34(3), 459-470. https://doi.org/10.1590/S1519-566X2005000300015
Corrales-Lerma, R., Avendaño-Arrazate, C., Morales-Nieto, C.,
Santellano-Estrada, E., Villarreal-Guerrero, F., Melgoza-Castillo, A.,
Álvarez-Holguín, A. & Gómez-Simuta, Y. (2019).
Radiación gamma para inducción de mutagénesis en pasto rosado
(Melinis repens (Willd.) Zizka). Acta Universitaria, 29, 1-10. https://doi.org/10.15174/au.2019.1847.
Cobos, F., Gómez, L., Reyes Borja, W., Hasang, E., Ruilova, M. & Duran, P. (2021). Effects of salinity levels in Oryza sativa in different phenological stages under greenhouse conditions. Revista de la Facultad de Agronomía de la Universidad del Zulia, 39(1), e223905. https://doi.org/10.47280/RevFacAgron(LUZ).v39.n1.05.
Costa, A. (2017). Indución y evaluación de mutantes de alcachofa tolerantes a salinidad. Universitas Miguel Hernández. http:// http://dspace.umh.es/bitstream/11000/3445/1/Costa%20Ortiz%2C%20Aida%20TFGBiotec%202014-15.pdf.
Emrani, S., Arzani, A. & Saeidi, G. (2011). Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens. African Journal of Biotechnology, 10(59), 12602-12613. https://doi.org/10.5897/AJB11.329.
Gómez, J., Aguirre, L., Gómez, L., Reyes, W., Rodríguez, J. & Arana, L. (2020). Dosis letal media para inducir mutaciones, con rayos gamma, en pastojaneiro (Eriochloa polystachya Kunth). Revista de Producción Animal, 32(1), 73-83. https://revistas.reduc.edu.cu/index.php/rpa/article/view/e339.
González, M. (2014). Desarrollo de una plataforma de tilling en melón (Cucumis melo L.). Tesis Doctoral. Universitat Autónoma de Barcelona, Barcelona, España. 282 p. https://ddd.uab.cat/record/127025.
Greene, E., Codomo, C., Taylor, N., Henikoff, J., Till, B., Reynolds, S., Enns L., Burtner C., Johnson, J., Odden, A. & Steven, L. (2003). Spectrum of Chemically induced mutations from large-scale reverse genetic screening in Arabidopsis. Genetics, 164(2), 731–740. https://doi.org/10.1093/genetics/164.2.731.
Hasang, E., Gómez, J., Moreira, E. & Cobos, F. (2020). Variabilidad fenotípica, y desarrollo de estolones de pasto Janeiro (Eriochloa polystachya Kunth), irradiados a 52 gy de rayos gamma (60co). Journal of Science and Research, 5, 96-109. https://revistas.utb.edu.ec/index.php/sr/article/view/1000.
Khalil, F., Naiyan, X., Tayyab, M. & Pinghua, C. (2018). Screening of EMS-Induced Drought-Tolerant Sugarcane Mutants Employing Physiological, Molecular and Enzymatic Approaches. Agronomy, 8(10), 226. doi:https://doi.org/10.3390/agronomy8100226.
Konzak, C., Nilan, R., Wagner, T. & Foster, R. (1965). Efficient chemical mutagenesis, the use of induced mutations in Plant Breeding (Rep. FAO/IAEA Tech. Meeting Rome, 1964). Porgamon Press, Oxford. p. 49–70.
Kumar, G. & Pandey, A. (2020). Ethyl metha- sulfonate-induced changes in cyto-morphological and biochemical aspects of Coriandrum sativum L. Journal of the Saudi Society of Agricultural Sciences, 18(4), 469-475. https://doi.org/10.1016/j.jssas.2018.03.003.
López, E. (2011). Inducción de variabilidad genética para tolerancia a estreses abióticos mediante técnicas de cultivo in vitro en Cenchrus ciliaris L. Universidad Internacional de Andalucía, España. 113 p. https://core.ac.uk/download/pdf/72021262.pdf.
Mendoza, R. (2020). Inducción mutagénica en cultivares de Eustoma grandiflorum mediante el uso de metanosulfonato de etilo (EMS). Centro de Investigación y Asistencia en Tecnología y
diseño del estado de Jalisco, A.C, México. https://ciatej.repositorioinstitucional.mx/jspui/bitstream/1023/746/1/Rafael%20Mendoza%20G%C3%B3mez%20Bio.pdf.
Mostafa, G. (2015). Effect of some chemical mutagens on growth, phytochemical composition and the induction of mutations in Khaya senegalensis. International Journal of Plant Breeding and Genetics, 9(2), 57-67. https://doi:10.3923 / ijpbg.2015.57.67.
Nobel, P. (1999). Physicochemical & Environmental. Plant Physiology. Second Edition. Academic Press. San Diego, California, USA. 474 p. https://books.google.co.ve/books?id=yW_pptyiMHoC&dq=Nobel,+P.+(1999).&lr=&hl=es&source=gbs_navlinks_s.
Pankhurst, C, Blair, M & Broughton, W. (2004). Tilling the beans. In Proceedings of the 3rd International Scientific Meeting, Phaseomics III, pp. 13-15.
Porch, T., Blair, M., Lariguet, P., Galeano, C., Pankhurst, C. & Broughton, W. (2009). Generation of a Mutant Population for TILLING Common Bean Genotype BAT 93. Journal of the American Society for Horticultural Science, 134(3), 348-355. https://doi.org/10.21273/JASHS.134.3.348.
Rodríguez, N., Torres, C., Chaman, M. & Hidalgo J. (2019). Efecto del estrés salino en el crecimiento y contenido relativo del agua en las variedades IR-43 y amazonas de Oryza sativa "arroz" (Poaceae). Arnaldoa, 26(3), 931-942. https://dx.doi.org/10.22497/arnaldoa.263.26305.
Rojas, L., Collado, R., León, A., Rivero, L., Ocaña, B., Hernández, M., Veitía, N., Martirena, A., Torres, D. & García, L. (2016). Concentración óptima de metano sulfonato de etilo en Phaseolus vulgaris L. cv. ‘DOR 364’ para inducir variaciones fenotípicas. Biotecnología Vegetal, 16(3), 179-188. https://revista.ibp.co.cu/index.php/BV/article/view/526.
Sharma, S., Sood, R & Pandey, D. (2005). Studies on mutagen sensitivity, effectiveness and efficiency in urdbean [Vigna mungo (L.) Hepper]. Indian Journal of Genetics and Plant Breeding, 65(1), 20–22. https://www.isgpb.org/journal/index.php/IJGPB/article/view/1602.
Sotelo, G., Cardona, C & Miles, J. (2003). Desarrollo de híbridos de Brachiaria resistentes a cuatro especies de salivazo (Homoptera: Cercopidae). Revista Colombiana de Entomología, 29 (2): 157-163. https://doi.org/10.25100/socolen.v29i2.9599.
Suthakar, V. & Mullainathan, L. (2015). Studies on Effect of Physical and Chemical Mutagens in Sorghum (Sorghum bicolor (L.) Moench) in M2 Generation". International Letters of Natural Sciences, 37, 46-50. https://doi.org/10.18052/www.scipress.com/ILNS.37.46.
Yadav, P., Meena, H. S., Meena, P. D., Kumar, A., Gupta, R., Jambhulkar, S. & Singh, D. (2016). Determination of LD50 of ethyl methanesulfonate (EMS) for induction of mutations in rapeseed-mustard. Journal of Oilseed Brassica, 1(1), 77-82. http://srmr.org.in/ojs/index.php/job/article/download/33/32.
Publicado
2022-08-03
Como Citar
Gómez Villalva, J., Cobos Mora, F., Hasang Moran, E., Eguiluz de la Barra, A., & Cortez Herrera, I. (2022). Mutagênese induzida em grama de janeiro por etil-meta-sulfonato e seu efeito no controle da cigarrinha. Revista Da Faculdade De Agronomia Da Universidade De Zulia, 39(3), e223938. Obtido de https://produccioncientificaluz.org/index.php/agronomia/article/view/38556
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Direitos de Autor (c) 2022 Juan Gómez Villalva; Fernando Cobos Mora; Edwin Hasang Moran; Ana Luzmeira Eguiluz de la Barra y Isrrael Cortez Herrera
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