Bacterias con actividad antifúngica asociadas a Ocimum basilicum L.
Palabras clave:
Bacillus spp., albahaca, Aspergillus spp., Neoscytalidium dimidiatum, Alternaria spp., bacteria antifúngica
Resumen
La presencia de patógenos, como hongos, es una de las causas más importantes de pérdida de cultivos de albahaca en todo el mundo; sin embargo, muchos microorganismos tienen un papel crucial en el desarrollo de las plantas, incluida la protección contra los patógenos. En el presente estudio, se aislaron, cuantificaron y preservaron las bacterias predominantes asociadas a albahaca. La caracterización de las bacterias aisladas mostró 165 cepas Gram positivas, 152 con morfología bacilar y 13 con cocoide. Posteriormente, se realizaron ensayos de antagonismo in vitro, primero en contra Aspergillus spp. y posteriormente en contra de Neoscytalidium dimidiatum, Alternaria, spp. y Aspergillus spp. Finalmente, ensayos para evaluar el efecto de las bacterias aisladas sobre la germinación de semillas y las primeras etapas de desarrollo de albahaca fueron llevados cabo. Las plantas de albahaca muestreadas, producidas alrededor de La Paz, Baja California Sur, México, fueron colonizadas por bacterias antagonistas conocidas del género Bacillus. Las cepas de Bacillus amyloliquefaciens fueron las que presentaron de forma predominante la actividad antifúngica. Además, las cepas ALMH42, ALMR73 y ALAH91 no mostraron ningún efecto deletéreo sobre el desarrollo de las plántulas de albahaca. La exploración del potencial biotecnológico de estas cepas aisladas de plantas sanas de albahaca es de gran interés para futuras aplicaciones en este y otros cultivos.
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Banchio, E., X. Xie, H.Zhang, and P. W. Paré. 2009. Soil bacteria elevate essential oil accumulation and emissions in sweet basil. J. Agric. Food Chem., 57: 653-657. https://cutt.ly/znRmSq2
Broda, D.M., P.A. Lawson, R.G. Bell and D.R. Musgrave. 1999. Clostridium frigidicarnis sp. nov., a psychrotolerant bacterium associated with ‘blown pack’ spoilage of vacuum-packed meats. Int. J. Syst. Bacteriol., 49: 1539-1550. https://cutt.ly/snRmDTh
Chen, X. H., A. Koumoutsi, R.Scholz, and R. Borriss, 2009. More than anticipated - production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42. MIRCEN J. Appl. Microbiol. Biotechnol., 16: 14-24. https://cutt.ly/4nRmHmY
Daungfu, O., S. Youpensuk and S. Lumyong. 2019. Endophytic bacteria isolated from citrus plants for biological control of Citrus Canker in lime plants. Tropical Life Sciences Research, 30: 73-88. https://cutt.ly/nnRmKkq
Gilardi, G., S. Demarchi, A.Garibaldi, and M. L. Gullino, 2013. Management of downy mildew of sweet basil (Ocimum basilicum) caused by Peronospora belbahrii by means of resistance inducers, fungicides, biocontrol agents and natural products. Phytoparasitica, 41: 59-72. https://cutt.ly/4nRmL64
Goldman, E. and L.H. Green (Ed.) 2009. Practical Handbook of Microbiology, Second Edition. 2nd ed. London, pp.3-63. ISBN 978-0-8493-9365-5
Gowtham, H.G., M. Murali, S. B.Singh, T. R. Lakshmeesha, K.Narasimha Murthy, K. N. Amruthesh and S. R. Niranjana. 2018. Plant growth promoting rhizobacteria Bacillus amyloliquefaciens improves plant growth and induces resistance in chilli against anthracnose disease. BIOL CONTROL., 126: 209-217. https://cutt.ly/QnRmC4n
Hernández-Montiel, L.M., T. Rivas-García, M. Romero-Bastidas, C. J. Chiquito-Contreras, F. H. Ruiz-Espinoza and R. G. Chiquito-Contreras. 2018. Potencial antagónico de bacterias y levaduras marinas para el control de hongos fitopatógenos. Rev. Mexicana cienc. agric., 20: 4311-4321. https://cutt.ly/JnRmBDJ
Jacobs, J.L., T. L. Carroll and G. W. Sundin, 2005. The role of pigmentation, ultraviolet radiation tolerance, and leaf colonization strategies in the epiphytic survival of phyllosphere bacteria. Microb. Ecol, 49: 104-113. https://cutt.ly/CnRmN5H
Joo, H.J., H.Y. Kim, L.H. Kim, S.Lee, J.G. Ryu, and T.A. Lee, 2015. Brevibacillus sp. antagonistic to mycotoxigenic Fusarium spp. BIOL CONTROL., 87: 64–70. https://cutt.ly/enRm2fT
Kim, M.J., R. Radhakrishnan, S.M. Kang, Y.H. You, E.J. Jeong, J.G. Kim and I.J. Lee, 2017. Plant growth promoting effect of Bacillus amyloliquefaciens H-2-5 on crop plants and influence on physiological changes in soybean under soil salinity. Physiology and Molecular Biology of Plants, 23: 571-580. https://cutt.ly/TnRm9LI
Liu, Z., A. Budiharjo, P. Wang, H. Shi, J. Fang, R. Borriss, K. Zhang, and X. Huang, 2013. The highly modified microcin peptide plantazolicin is associated with nematicidal activity of Bacillus amyloliquefaciens FZB42. Applied Microbiology and Biotechnology, 97: 10081-10090. https://cutt.ly/bnRm8th
Lugtenberg, B. and F. Kamilova. 2005. Plant-growth-promoting rhizobacteria. Annu. Rev. Microbiol., 63, 541-556. https://cutt.ly/jnRm40B
Moffat, A.S. 2001. Finding new ways to fight plant diseases. Science, 292: 2270-2273. https://cutt.ly/nnRm6p0
Pieterse, C. M. J., C. Zamioudis, R. L. Berendsen, D. M. Weller, S. C. M. Van Wees and P. A. H. M. Bakker. 2014. Induced systemic resistance by beneficial microbes. Annu Rev Phytopathol, 52: 347-375. https://cutt.ly/DnRQqHm
Pound, M., A. French, J. Atkinson, D. Wells, M. Bennett and T. Pridmore, 2013. RootNav: Navigating Images of Complex Root Architectures. Plant Physiology, 162: 1802-1814. https://cutt.ly/snRQeXx
Purahong, W., L. Orrù, I. Donati, G. Perpetuini, A. Cellini, A. Lamontanara, V. Michelotti, G. Tacconi, and F. Spinelli. 2018. Plant microbiome and its link to plant health: host species, organs, and Pseudomonas syringae pv. actinidiae infection shaping bacterial phyllosphere communities of kiwifruit plants. Front Plant Sci, 9: 1563. https://cutt.ly/EnRQpyM
Qin, Y., Q. Shang, Y. Zhang, P. Li and Y.Chai, 2017. Bacillus amyloliquefaciens L-S60 reforms the rhizosphere bacterial community and improves growth conditions in cucumber plug seedling. Front Microbiol, 8: 2620. https://cutt.ly/CnRQfcV
Raaijmakers, J. M., I. De Bruijn, O. Nybroe and M. Ongena. 2010. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiology Reviews, 34, 1037-1062. https://cutt.ly/qnRQhPj
Rabbee, M. F., M. S. Ali, J. Choi, B. S. Hwang, S. C. Jeong and Baek K. 2019. Bacillus velezensis: a valuable member of bioactive molecules within plant microbiomes. Molecules, 24: 1046. https://cutt.ly/5nRQjB6
Rios-Velasco, C., J. N. Caro-Cisneros, D. I. Berlanga-Reyes, M. F. Ruíz-Cisneros, J. J. Ornelas-Paz, M. A. Salas-Marina, E. Villalobos-Pérez and V. M. Guerrero-Prieto, 2016. Identification and antagonistic activity in vitro of Bacillus spp. and Trichoderma spp. isolates against common phytopathogenic fungi. Rev. Mex. Fitopatol., 34: 84-99. https://cutt.ly/InRQHuE
Statistical Analysis System (SAS) Software, University Edition; Retrieved May 2, 2020, from. https://cutt.ly/tnRQLDq
Smith, D. L., Praslickova, D., & Ilangumaran, G. 2015. Inter-organismal signaling and management of the phytomicrobiome. Front Plant Sci, 6: 722. https://cutt.ly/CnRQCVT
Publicado
2021-10-01
Cómo citar
Arce-Amezquita, P. M., Romero-Bastidas, M., & Rojas-Contreras, M. (2021). Bacterias con actividad antifúngica asociadas a Ocimum basilicum L. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 38(4), 913-933. Recuperado a partir de https://produccioncientificaluz.org/index.php/agronomia/article/view/36802
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Sección
Producción Vegetal
Derechos de autor https://creativecommons.org/licenses/by-nc/4.0/
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
