Reaction of coffee cultivars to Hemileia vastatrix Berk. & Broome strains in Venezuela, under controlled conditions

• Emma Ramírez Universidad Centroccidental Lisandro Alvarado. Postgrado de Fitopatología, Barquisimeto, Lara, Venezuela. https://orcid.org/0009-0009-6445-7978
• Dorian Rodríguez Universidad Centroccidental Lisandro Alvarado. Postgrado de Fitopatología, Barquisimeto, Lara, Venezuela. https://orcid.org/0000-0002-0538-7094
• Alexander Hernández Universidad Centroccidental Lisandro Alvarado. Postgrado de Fitopatología, Barquisimeto, Lara, Venezuela. http://orcid.org/0000-0002-8914-6238

Keywords: resistance, coffee rust, race, pathotypes

Abstract

Coffee rust resistance is of great importance in worldwide coffee crops, since its biotrophic pathogen shows, currently, more than 55 identified races with diverse virulent genes. This work was based on the determination of the reaction of five commercial coffee cultivars inoculated, under controlled conditions, with three strains of Hemileia vastatrix (race XXXIX and pathotypes Hv01ve and Hv02ve) previously identified in Venezuela. Three velvet leaves taken from six selected plants of five cultivars (Caturra, Catuai amarillo, Monteclaro, Colombia 27 y Castillo) were inoculated with 80 μL of a uredospore dilution (5 x 105 uredospores.mL-1), and incubated at 22 °C +/- 2 °C and 12 h light photoperiod. Incubation period, latency period, infection grade, number of initial lesions, number of sporulated lesions, area of lesion, and area of sporulated lesion were the variables used for evaluation. Results showed that number of initial lesion, number of sporulated lesion and area of sporulated lesion allowed to determine the presence of incomplete resistance in Castillo to race XXXIX, Castillo, Catuai and Colombia 27 to Hv01ve, and Monteclaro to Hv02ve, trials also showed possible complete resistance in Monteclaro to race XXXIX and Colombia 27 to Hv02ve. It is suggested that these results should be taken into account when selecting the coffee cultivars to be planted.

References

Avelino, J., & Rivas, G. (2013). La roya anaranjada del cafeto. HAL Id: hal-01071036. https://hal.science/Hal-01071036/
Avelino, J., Allinne, C., Cerda, R., Willocquet, L., & Savary, S. (2018). Multiple-disease system in coffee: From crop loss assessment to sustainable management. Annual Review of Phytopatholgy, 56, 611-635. https://doi.org/10.1146/annurev-phyto-080417-050117
Burbano-Figueroa, O. (2020). Resistencia de plantas a patógenos: una revisión sobre los conceptos de resistencia vertical y horizontal. Revista Argentina de Microbiología, 52(3), 245-255. https://doi.org/10.1016/j.ram.2020.04.006
Bustamante, J., Sarmiento, A., Casanova, A., Contreras, E., Yánez, C., Romero, C., Peña, I., Verenzuela, A., Morales, N., Garnica, J., & Colmenares, N. (2001). Caracterización de resistencia incompleta a Hemileia vastatrix en genotipos de café (Coffea arabica L.) cultivar Bramón I. Bioagro, 13(2), 65-70. https://dialnet.unirioja.es/servlet/articulo?codigo=1288924.
Capucho, A., Teixeira Caixeta, E., Maciel-Zambolim, E., & Zambolim, L. (2009). Herança da resistência do Híbrido de Timor UFV 443-03 à ferrugem-do-cafeeiro. Pesquisa Agropecuaria Brasileira, 44(3), 276-282. https://doi.org/10.1590/S0100-204X2009000300009
Coelho de Sousa, I., Nascimento, M., Nunes, G., Campana, A., Damião, C., Fonseca, F., Pires, D., Nogueira, K., Ferreira, C., Zambolim, L., &Teixeira, E. (2020). Genomic prediction of leaf rust resistance to Arabica coffee using machine learning algorithms. Scientia Agricola, 78(4), e20200021. http://doi.org/10.1590/1678-992X-2020-0021.
Dallangnol, L., & Vieira de Araujo Filho, J.V. (2018). Resistência de plantas a microrganismos. En: L. J. Dallagnol (Ed.), Resistência genética de plantas a patógenos (pp. 126–149). Editora UFPel. https://www.researchgate.net/publication/329210299
Deepak, K., Hanumantha, B.T., & Sreenat, H.L. (2012). Viability of coffee leaf rust (Hemileia vastatrix) uredinospores stored at different temperatures. Journal of Biotechnology and Biomaterials, 2, 143. http://doi.org/10.4172/2155-952X.1000143
Eskes, A.B. (1983). Characterization of incomplete resistance to Hemileia vastatrix in the Icatu coffee population Coffea arabica x Coffea canephora. Euphytica, 32(2), 649-658. https://doi.org/10.1007/BF00021477
InfoStat. 2020. InfoStat versión 2020. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. http://www.infostat.con.ar
Lima, I.S., Freitas-Lopes, R., Ferreira D.S., S., Ferreira Maciel, Florez, J.C., Zambolim, E.M., Zambolim, L., & Teixeira C., E. (2022). Transcriptome analysis uncovers the gene expression profile of Hemileia vastatrix (Race XXXIII) during the interactions with resistant and susceptible coffee. Agronomy, 12(2), 444. https://doi.org/10.3390/agronomy12020444
Lizardo-Chavez, Cristian Y., Herrera-Zelaya, Diana A., Buezo-Mejía, Marco, & Tróchez-Fernández, Hildebrando. (21-24 de Julio 1992). Resistencia horizontal a la roya del café (Hemileia vastatrix Berk. & Br.) de las variedades comerciales de café de Honduras. XV Simposio Latinoamericano de Caficultura, Xalapa, México.
Pires, D., Caixeta, E.T., Moreira, K.F., de Oliveira, A.C.B., de Freitas, K.N.P., Pereira, A.A., Rosado, R.D.S., Zambolim, L., & Cruz, C.D. (2021). Marker-assisted pyramiding of multiple disease resistance genes in coffee genotypes (Coffea arabica). Agronomy, 11, 1763. https://doi.org/10.3390/agronomy11091763
Pires, D., Lima Castro, I. S., de Oliveira Mendes, T. A., Rodríguez Alves, D., Dugassa Barka, G., Rossi Marques Barreiros, Zambolim, L., Sussumu Sakiyama N., & Teixeira Caixeta. E. (2020). Receptor-like kinase (RLK) as a cantidate gene conferring resistance to Hemileia vastatrix in coffee. Scienta Agrícola, 78(6), e20200023. http://doi.org/10.1590/1678-992X-2020-0023
Quiroga-Cardona, J. (2021). La resistencia incompleta del café a la roya: una revisión. Revista Cenicafé, 72(2), e72208. https://doi.org/10.38141/10778/72208
Ramírez-Poletto, E., Rodríguez G., D. A., Zambolim, L., & Granados, E. (2024). Identificación de cepas fisiológicas de Hemileia vastatrix en el estado Táchira, Venezuela. Bioagro, 36(3), 277-286. https://doi.org/10.51372/bioagro363.3
Rios, J. A., & Debona, D. (2018). Efeito epidemiológico da resistência de hospedeiro. En: L.J. Dallagnol (Ed.), Resistência genética de plantas a patógenos (pp: 126–149). Editora UFPel.
Schulze-Lerfert, P., & Panstruga, R. (2003). Establishment of biotrophy by parasitic fungi and reprogramming of host cells for disease resistance. Annual Review Phytopathoy, 41, 641–667. http://doi.org/10.1146/annurev.phyto.41.061002.083300
Silva, M.d.C., Guerra-Guimarães, L., Diniz, I., Loureiro, A., Azinheira, H., Pereira, A.P., Tavares, S., Batista, D., & Várzea, V. (2022). An overview of the mechanisms involved in coffee-Hemileia vastatrix interactions: Plant and pathogen perspectives. Agronomy, 12(2), 326.https://doi.org/10.3390/agronomy12020326
Talhinhas, P., Batista, D., Diniz, I., Vieira, A., Silva, D.N., Loureiro, A., Tavares, S., Pereira, A.P., Azinheira, H.G., Guerra- Guimarães, L., Várzea, V., & Silva d.C., M. (2017). The coffee leaf rust pathogen Hemileia vastatrix: One and a half centuries around the tropics. Molecular Plant Pathology, 18(8), 1039–1051. https://doi.org/10.1111/mpp.12512
Várzea, V., Pereira, A.P., & Silva, M.d.C. (2023). Screening for resistance to coffee Leaf Rust. En: I.L. Ingelbrecht, M.d.C.L. Silva & J. Jankowicz-Cieslak, (Eds.), Mutation breeding in coffee with special reference to Leaf Rust (pp. 209-224).Springer. https://doi.org/10.1007/978-3-662-67273-0_15