Association of gene expression with blood metabolites and fatty acid profile in lambs
Keywords:
gene expression, gene association, lipid metabolism, lamb
Abstract
Some agricultural byproducts can be used as viable alternatives in animal feed. Therefore, the effect of the inclusion of avocado meal and sunflower oil on the profile of fatty acids, blood metabolites and the expression of genes associated with lipid metabolism in lambs was evaluated. Three treatments were evaluated: 0 % control, 10 % avocado meal and 10 % sunflower oil for 84 days. It was found that including avocado meal in the diet increased the amount of blood glucose and also globulin, but decreased creatinine and Glutamic Oxaloacetic Transaminase, compared to the addition of sunflower oil where the content of cholesterol, triglycerides, and VLDL decreased. and R A/G. There was no difference for muscle fat percentage. The concentration of fatty acids C20:0, C20:1 and C23:0 was lower with avocado meal, without affecting the MUFA and UFA values, the UFA/SFA ratio and the saturation index. The inclusion of avocado meal caused a change in the expression of the ACACA, FASN, SCD, FASBP3, PPARG and SREBF1 genes in the Longisimus thoracis muscle, there was a positive association between glucose and the FASBP3 gene, also of HDL with the PPARG gene, MUFA, proteins and indices of desaturation of fatty acids were associated with genes such as SCD, FASN, SREBF1 and ACACA The inclusion of avocado meal is an alternative to modify genetic expression, also to reduce very low density cholesterol values in the blood.
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References
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Kostik, V., Memeti, S. & Bauer, B. (2013). Fatty acid composition of edible oils and fats. Journal of Hygienic Engineering and Design, 4, 112-116. https://eprints.ugd.edu.mk/11460/1/06. %20Full %20paper %20- %20Vesna %20Kostik %202.pdf
Lemus-Avalos, G., Lemus-Flores, C., Bugarín-Prado, J. O., Grageola-Núñez, F., Ayala-Valdovinos, M. A., Duifhuis-Rivera. T., Moo-Huchin, V. M. & Dzib-Cauich, D. (2020). Effect of diets with avocado meal on lipids in muscle, antioxidants and gene expression in finished pigs. Revista BioCiencias, 7, e968. https://doi.org/10.15741/revbio.07.e968
Lemus-Flores, C., Bugarín-Prado, J. O., Grageola Nuñez, F., Valdivia Bernal, R., Ruiz Dimas, I., Bonilla Cardenas, J. A. & Segura Correa, J. C. (2020). The effect of avocado flour, sunflower oil and different forage: concentrate ratios in the final diet on feed intake, digestibility and productive performance of male sheep. Veterinarski Arhiv, 90(4), 353-364. https://hrcak.srce.hr/file/356220
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Mapiye, C., Aalhus, J. L., Turner, T. D., Rolland, D. C., Basarab, J. A., Baron, V. S., McAllister, T. A., Block, H. C., Uttaro, B., Lopez-Campos, O., Proctor, S. D. & Dugan, M. E. R. (2013). Effects of feeding flaxseed or sunflower-seed in high-forage diets on beef production, quality and fatty acid composition. Meat Science, 95, 98-109. https://doi.org/10.1016/j.meatsci.2013.03.033
Miltko, R., Majewska, M. P., Bełżecki, G., Kula, K. & Kowalik, B. (2019). Growth performance, carcass and meat quality of lambs supplemented different vegetable oils. Asian-Australas Journal Animal Science. 32(6), 767-775. https://doi.org/10.5713/ajas.18.0482
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Rodrigues Cavalcante, I. T., Hauss de Sousa, W., Ribeiro, N. L., Queiroga Cartaxo, F., de Farias Ramos, J. P. & de Azevedo, P. S. (2022). Animal feed based on forage cactus: use of viscera in traditional dishes. Production of by-products that can result in an economic return. Food Science and Technology, 42, e105921. https://doi.org/10.1590/fst.105921
Romero-Bernal, J., Morales Almaraz, E., Castelán Ortega, O. O., Pescador Salas, N. & González-Ronquillo, M. (2017). Chemical composition and fatty acid profile in meat from grazing lamb diets supplemented with ryegrass hay, fishmeal and soya bean meal as PUFA sources. Ciencia Rural Santa María, 47(4), e20160533. http://dx.doi.org/10.1590/0103-8478cr20160533
Ruíz-Dimas, I., Lemus-Flores, C., Bonilla-Cárdenas, J. A., Rodríguez-Carpena, J. G., Valdivia-Bernal, R., Grageola-Núñez, F. & Bugarin-Prado. J. O. (2022). Acceptability of meal of avocado full fruit included in the diet for sheep and pigs. Brazilian Journal of Animal and Environmental Research, Curitiba, 5(1), 193-199. https://doi.org/10.34188/bjaerv5n1-015
Santana, G. y Correa, H. J. (2016). Inclusión de tres fuentes lipídicas en la dieta de vacas lactantes bajo pastoreo sobre el consumo y la digestibilidad. Livestock Research for Rural Development, 28(3), 38. http://www.lrrd.org/lrrd28/3/sant28038.html
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Steibel, J. P., Poletto, R., Coussens, P. M. & Rosa, G. J. M. (2009). A powerful and flexible linear mixed model framework for the analysis of relative quantification RT-PCR data. Genomics, 94(2), 146-152. https://doi.org/10.1016/j.ygeno.2009.04.008
Wang, X., Yan, S., Shi, B., Zhang, Y. & Guo, X. (2019). Effects of concentrate supplementation on fatty acid composition and expression of lipogenic genes of meat and adipose tissues in grazing lambs. Italian Journal of Animal Science, 18(1), 554-563. https://doi.org/10.1080/1828051X.2018.1547666
Yue, Y., Cheng, X., Zhao, S. G., Liu, Z., Liu, L. S., Zhou, R., Wu, J. P. & Brown, M. A. (2016). Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep. Genet Molecular Research, 15(1), 1-10. http://dx.doi.org/10.4238/gmr.15017323
Zsédely, E., Király, A., Szabó, C. S., Németh, K., Dóka, O. & Schmidt, J. (2012). Effect of dietary linseed oil soap on lamb meat. World Academy Science Engineering and Technology, 6(3), 67-70. https://publications.waset.org/13519/pdf
Araujo, R. G., Rodriguez-Jasso, R. M., Ruiz, H. A., Pintado, M. M. E. & Aguilar, C. N. (2018). Avocado by-products: nutritional and functional properties. Trends in Food Science & Technology, 80, 51-60. https://doi.org/10.1016/j.tifs.2018.07.027
Astuti, D. A., Babab, A. S. & Wibawanc, I. W. T. (2011). Rumen Fermentation, Blood Metabolites, and Performance of Sheep Fed Tropical Browse Plants. Media Peternakan, 34(3), 201-206. https://doi.org/10.5398/medpet.2011.34.3.201
Avellanet, R., Cuenca, R., Pastor, J. y Jordana, J. (2007). Parámetros hematológicos y bioquímico clínicos en la raza ovina xisqueta. Archivos de Zootecnia, 56(Sup. 1), 497-501. https://www.redalyc.org/pdf/495/49509922.pdf
de Castro Budel, J. C., Gomes de Castro, V. C., Mota de Souza, S., de Souza Nahúm, B., Correa Barbosa, A. V., Silva Rodrigues, L., Berndt, A., Navajas Rennó, L., de Morais, E., Rodrigues da Silva, J. A., de Carvalho Rodrigues, T. C. G., Maciel e Silva, A. G. & Lourenço-Junior, J. B. (2023). Methane emission, intake, digestibility, performance and blood metabolites in sheep supplemented with cupuassu and tucuma cake in the eastern Amazon. Frontier in Veterinary Science, 10, 1106619. https://doi.org/10.3389/fvets.2023.1106619
Bugarín-Prado, J. O., Lemus-Flores, C., Grageola-Núñez, F., Valdivia-Bernal, R. y Bonilla-Cárdenas, J. (2021). Efecto de suplementación con harina de aguacate en dietas de corderos sobre crecimiento y el rendimiento de la canal. Abanico Veterinario, 11, 1-12. http://dx.doi.org/10.21929/abavet2021.32
de Evan, T., Carro, M. D., Fernández, Yepes .J. E., Haro, A., Arbesú, L., Romero-Huelva, M. & Molina-Alcaide, E. (2020). Efects of Feeding Multinutrient Blocks Including Avocado Pulp and Peels to Dairy Goats on Feed Intake and Milk Yield and Composition. Animals, 10(2), 194. https://doi.org/10.3390/ani10020194
Díaz, M. T., Álvarez, I., De la Fuente, J., Sañudo, C., Campo, M. M., Oliver, M. A., Font i Furnols, M., Montossi, F., San Julián, R., Nute, G. R. & Cañeque, V. (2005). Fatty acid composition of meat from typical lamb production systems of Spain, United Kingdom, Germany and Uruguay. Meat Science, 71(2), 256–263. https://doi.org/10.1016/j.meatsci.2005.03.020
Folch, J., Lees, M., & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipides from animal tissues. The Journal of biological chemistry, 226(1), 497–509. http://dx.doi.org/10.1016/S0021-9258(18)64849-5
Fránquez, P., Rodríguez, G., Lemus, C., Grageola, F. & Ly, J. (2017). Performance traits and índices of the intake pattern of fattened pigs with fresh paste of whole avocado. Cuban Journal of Agricultural Science, 51(3), 329-336. http://cjascience.com/index.php/CJAS/article/view/755
González-Jiménez, A. C., Mejía-Martínez, K., Becerra-Verdín, E. M., Bugarín-Prado, J. O. & Lemus-Flores. C. (2021). Carcass characteristics and blood biochemical profiles in finishing pigs fed with different levels of avocado paste (Persea americana mill.). Brazilian Journal of Animal and Environmental Research, 4(2), 2344-2359. https://doi.org/10.34188/bjaerv4n2-064
Hernández-López, S. H., Rodríguez-Carpena, J. G., Lemus-Flores, C., Grageola-Nuñez, F. & Estévez, M. (2016). Avocado waste for finishing pigs: Impact on muscle composition andoxidative stability during chilled storage. Meat Science, 116, 186-192. https://doi.org/10.1016/j.meatsci.2016.02.018
Kostik, V., Memeti, S. & Bauer, B. (2013). Fatty acid composition of edible oils and fats. Journal of Hygienic Engineering and Design, 4, 112-116. https://eprints.ugd.edu.mk/11460/1/06. %20Full %20paper %20- %20Vesna %20Kostik %202.pdf
Lemus-Avalos, G., Lemus-Flores, C., Bugarín-Prado, J. O., Grageola-Núñez, F., Ayala-Valdovinos, M. A., Duifhuis-Rivera. T., Moo-Huchin, V. M. & Dzib-Cauich, D. (2020). Effect of diets with avocado meal on lipids in muscle, antioxidants and gene expression in finished pigs. Revista BioCiencias, 7, e968. https://doi.org/10.15741/revbio.07.e968
Lemus-Flores, C., Bugarín-Prado, J. O., Grageola Nuñez, F., Valdivia Bernal, R., Ruiz Dimas, I., Bonilla Cardenas, J. A. & Segura Correa, J. C. (2020). The effect of avocado flour, sunflower oil and different forage: concentrate ratios in the final diet on feed intake, digestibility and productive performance of male sheep. Veterinarski Arhiv, 90(4), 353-364. https://hrcak.srce.hr/file/356220
Ly, J., Fránquez, P., Rodríguez, G., Lemus, C., Domínguez, I. A. & Grageola, F. (2021). Note on in vitro digestion of avocado products for pigs. South African Journal of Animal Science, 51, 138–141. https://hdl.handle.net/10520/ejc-sajas_v51_n1_a16
Mapiye, C., Aalhus, J. L., Turner, T. D., Rolland, D. C., Basarab, J. A., Baron, V. S., McAllister, T. A., Block, H. C., Uttaro, B., Lopez-Campos, O., Proctor, S. D. & Dugan, M. E. R. (2013). Effects of feeding flaxseed or sunflower-seed in high-forage diets on beef production, quality and fatty acid composition. Meat Science, 95, 98-109. https://doi.org/10.1016/j.meatsci.2013.03.033
Miltko, R., Majewska, M. P., Bełżecki, G., Kula, K. & Kowalik, B. (2019). Growth performance, carcass and meat quality of lambs supplemented different vegetable oils. Asian-Australas Journal Animal Science. 32(6), 767-775. https://doi.org/10.5713/ajas.18.0482
Minitab 15 Statistical Software (Minitab v15). (2007). [Computer software]. State College, PA: Minitab, Inc. www.minitab.com
Mohan, N., Harihara, I., Babul, C., Madan, K., Anubrata, D. & Kalita, D. (2012). Effect of dietary sunflower oil and coconut oil on adipose tissue gene expression, fatty acid composition and serum lipid profile of grower pigs. Archives of Animal Nutrition, 66(4), 271-282. https://doi.org/10.1080/1745039X.2012.683324
Rodrigues Cavalcante, I. T., Hauss de Sousa, W., Ribeiro, N. L., Queiroga Cartaxo, F., de Farias Ramos, J. P. & de Azevedo, P. S. (2022). Animal feed based on forage cactus: use of viscera in traditional dishes. Production of by-products that can result in an economic return. Food Science and Technology, 42, e105921. https://doi.org/10.1590/fst.105921
Romero-Bernal, J., Morales Almaraz, E., Castelán Ortega, O. O., Pescador Salas, N. & González-Ronquillo, M. (2017). Chemical composition and fatty acid profile in meat from grazing lamb diets supplemented with ryegrass hay, fishmeal and soya bean meal as PUFA sources. Ciencia Rural Santa María, 47(4), e20160533. http://dx.doi.org/10.1590/0103-8478cr20160533
Ruíz-Dimas, I., Lemus-Flores, C., Bonilla-Cárdenas, J. A., Rodríguez-Carpena, J. G., Valdivia-Bernal, R., Grageola-Núñez, F. & Bugarin-Prado. J. O. (2022). Acceptability of meal of avocado full fruit included in the diet for sheep and pigs. Brazilian Journal of Animal and Environmental Research, Curitiba, 5(1), 193-199. https://doi.org/10.34188/bjaerv5n1-015
Santana, G. y Correa, H. J. (2016). Inclusión de tres fuentes lipídicas en la dieta de vacas lactantes bajo pastoreo sobre el consumo y la digestibilidad. Livestock Research for Rural Development, 28(3), 38. http://www.lrrd.org/lrrd28/3/sant28038.html
Statistical Product and Service Solutions (SPSS). (2011). IBM SPSS Statistics for Windows, Versión 20.0. Armonk, NY: IBM Corp. https://www.ibm.com/mx-es
Steibel, J. P., Poletto, R., Coussens, P. M. & Rosa, G. J. M. (2009). A powerful and flexible linear mixed model framework for the analysis of relative quantification RT-PCR data. Genomics, 94(2), 146-152. https://doi.org/10.1016/j.ygeno.2009.04.008
Wang, X., Yan, S., Shi, B., Zhang, Y. & Guo, X. (2019). Effects of concentrate supplementation on fatty acid composition and expression of lipogenic genes of meat and adipose tissues in grazing lambs. Italian Journal of Animal Science, 18(1), 554-563. https://doi.org/10.1080/1828051X.2018.1547666
Yue, Y., Cheng, X., Zhao, S. G., Liu, Z., Liu, L. S., Zhou, R., Wu, J. P. & Brown, M. A. (2016). Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep. Genet Molecular Research, 15(1), 1-10. http://dx.doi.org/10.4238/gmr.15017323
Zsédely, E., Király, A., Szabó, C. S., Németh, K., Dóka, O. & Schmidt, J. (2012). Effect of dietary linseed oil soap on lamb meat. World Academy Science Engineering and Technology, 6(3), 67-70. https://publications.waset.org/13519/pdf
Published
2024-02-04
How to Cite
Lemus , C., Bugarín, J., Lemus , G., Rodríguez , J., Peña, G., & Loya, J. (2024). Association of gene expression with blood metabolites and fatty acid profile in lambs. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 41(1), e244102. Retrieved from https://produccioncientificaluz.org/index.php/agronomia/article/view/41580
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Animal Production
Copyright (c) 2024 Clemente Lemus Flores, Job Oswaldo Bugarín Prado, Gilberto Lemus Avalos, Javier Germán Rodríguez Carpena, Gabriela Rosario Peña Sandoval, José Lenin Loya Olguin
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