Ocimum basilicum L. associated bacteria with antifungal activity

• Pablo Misael Arce-Amezquita Departamento Académico de Agronomía, Universidad Autónoma de Baja California Sur. Carretera al sur km 5.5, Col. El Mezquitito. 23080, La Paz, Baja California Sur. México. http://orcid.org/0000-0002-7938-9084
• Mirella Romero-Bastidas Departamento Académico de Agronomía, Universidad Autónoma de Baja California Sur. Carretera al sur km 5.5, Col. El Mezquitito. 23080, La Paz, Baja California Sur. México. https://orcid.org/0000-0002-6906-4243
• Maurilia Rojas-Contreras https://orcid.org/0000-0001-7326-1787

Keywords: Bacillus spp., basil, Aspergillus spp., Neoscytalidium dimidiatum, Alternaria spp., antifungal bacteria

Abstract

The presence of pathogens, like fungi, is one of the most important causes of basil crop loss around the world; however, many microorganisms have a crucial role on plant development including protection against pathogenic ones. In the present study, basil associated bacteria were isolated, quantified and preserved. Characterization of isolated bacteria showed 165 Gram positive strains, 152 with bacillary and 13 with coccoid morphology. Later, in vitro antagonism assays were performed, first against Aspergillus spp. and then against Neoscytalidium dimidiatum, Alternaria spp. and Aspergillus spp. Finally, the effect of the isolated bacteria on basil seed germination and first stages of development were carried out. Sampled basil plants, produced around La Paz, Baja California Sur, Mexico, were colonized by known antagonistic bacteria of the Bacillus genus. Bacillus amyloliquefaciens strains were the prevailing species with antifungal activity. Moreover, strains ALMH42, ALMR73 and ALAH91 did not show any deleterious effect on basil seedling development. Biotechnological potential exploration of these isolated strains from healthy basil plants is of great interest for future applications on this and other crops.

References

Adamović, D., I. Dalović and N. Mrkovački. 2015. Microbial abundance in rhizosphere of medicinal and aromatic plant species in conventional and organic growing systems. Ratarstvo i povrtarstvo, 52: 1-6. https://cutt.ly/znRmI2O
Banchio, E., X. Xie, H.Zhang, and P. W. Paré. 2009. Soil bacteria elevate essential oil accumulation and emissions in sweet basil. J. Agric. Food Chem., 57: 653-657. https://cutt.ly/znRmSq2
Broda, D.M., P.A. Lawson, R.G. Bell and D.R. Musgrave. 1999. Clostridium frigidicarnis sp. nov., a psychrotolerant bacterium associated with ‘blown pack’ spoilage of vacuum-packed meats. Int. J. Syst. Bacteriol., 49: 1539-1550. https://cutt.ly/snRmDTh
Chen, X. H., A. Koumoutsi, R.Scholz, and R. Borriss, 2009. More than anticipated - production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42. MIRCEN J. Appl. Microbiol. Biotechnol., 16: 14-24. https://cutt.ly/4nRmHmY
Daungfu, O., S. Youpensuk and S. Lumyong. 2019. Endophytic bacteria isolated from citrus plants for biological control of Citrus Canker in lime plants. Tropical Life Sciences Research, 30: 73-88. https://cutt.ly/nnRmKkq
Gilardi, G., S. Demarchi, A.Garibaldi, and M. L. Gullino, 2013. Management of downy mildew of sweet basil (Ocimum basilicum) caused by Peronospora belbahrii by means of resistance inducers, fungicides, biocontrol agents and natural products. Phytoparasitica, 41: 59-72. https://cutt.ly/4nRmL64
Goldman, E. and L.H. Green (Ed.) 2009. Practical Handbook of Microbiology, Second Edition. 2nd ed. London, pp.3-63. ISBN 978-0-8493-9365-5
Gowtham, H.G., M. Murali, S. B.Singh, T. R. Lakshmeesha, K.Narasimha Murthy, K. N. Amruthesh and S. R. Niranjana. 2018. Plant growth promoting rhizobacteria Bacillus amyloliquefaciens improves plant growth and induces resistance in chilli against anthracnose disease. BIOL CONTROL., 126: 209-217. https://cutt.ly/QnRmC4n
Hernández-Montiel, L.M., T. Rivas-García, M. Romero-Bastidas, C. J. Chiquito-Contreras, F. H. Ruiz-Espinoza and R. G. Chiquito-Contreras. 2018. Potencial antagónico de bacterias y levaduras marinas para el control de hongos fitopatógenos. Rev. Mexicana cienc. agric., 20: 4311-4321. https://cutt.ly/JnRmBDJ
Jacobs, J.L., T. L. Carroll and G. W. Sundin, 2005. The role of pigmentation, ultraviolet radiation tolerance, and leaf colonization strategies in the epiphytic survival of phyllosphere bacteria. Microb. Ecol, 49: 104-113. https://cutt.ly/CnRmN5H
Joo, H.J., H.Y. Kim, L.H. Kim, S.Lee, J.G. Ryu, and T.A. Lee, 2015. Brevibacillus sp. antagonistic to mycotoxigenic Fusarium spp. BIOL CONTROL., 87: 64–70. https://cutt.ly/enRm2fT
Kim, M.J., R. Radhakrishnan, S.M. Kang, Y.H. You, E.J. Jeong, J.G. Kim and I.J. Lee, 2017. Plant growth promoting effect of Bacillus amyloliquefaciens H-2-5 on crop plants and influence on physiological changes in soybean under soil salinity. Physiology and Molecular Biology of Plants, 23: 571-580. https://cutt.ly/TnRm9LI
Liu, Z., A. Budiharjo, P. Wang, H. Shi, J. Fang, R. Borriss, K. Zhang, and X. Huang, 2013. The highly modified microcin peptide plantazolicin is associated with nematicidal activity of Bacillus amyloliquefaciens FZB42. Applied Microbiology and Biotechnology, 97: 10081-10090. https://cutt.ly/bnRm8th
Lugtenberg, B. and F. Kamilova. 2005. Plant-growth-promoting rhizobacteria. Annu. Rev. Microbiol., 63, 541-556. https://cutt.ly/jnRm40B
Moffat, A.S. 2001. Finding new ways to fight plant diseases. Science, 292: 2270-2273. https://cutt.ly/nnRm6p0
Pieterse, C. M. J., C. Zamioudis, R. L. Berendsen, D. M. Weller, S. C. M. Van Wees and P. A. H. M. Bakker. 2014. Induced systemic resistance by beneficial microbes. Annu Rev Phytopathol, 52: 347-375. https://cutt.ly/DnRQqHm
Pound, M., A. French, J. Atkinson, D. Wells, M. Bennett and T. Pridmore, 2013. RootNav: Navigating Images of Complex Root Architectures. Plant Physiology, 162: 1802-1814. https://cutt.ly/snRQeXx
Purahong, W., L. Orrù, I. Donati, G. Perpetuini, A. Cellini, A. Lamontanara, V. Michelotti, G. Tacconi, and F. Spinelli. 2018. Plant microbiome and its link to plant health: host species, organs, and Pseudomonas syringae pv. actinidiae infection shaping bacterial phyllosphere communities of kiwifruit plants. Front Plant Sci, 9: 1563. https://cutt.ly/EnRQpyM
Qin, Y., Q. Shang, Y. Zhang, P. Li and Y.Chai, 2017. Bacillus amyloliquefaciens L-S60 reforms the rhizosphere bacterial community and improves growth conditions in cucumber plug seedling. Front Microbiol, 8: 2620. https://cutt.ly/CnRQfcV
Raaijmakers, J. M., I. De Bruijn, O. Nybroe and M. Ongena. 2010. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiology Reviews, 34, 1037-1062. https://cutt.ly/qnRQhPj
Rabbee, M. F., M. S. Ali, J. Choi, B. S. Hwang, S. C. Jeong and Baek K. 2019. Bacillus velezensis: a valuable member of bioactive molecules within plant microbiomes. Molecules, 24: 1046. https://cutt.ly/5nRQjB6
Rios-Velasco, C., J. N. Caro-Cisneros, D. I. Berlanga-Reyes, M. F. Ruíz-Cisneros, J. J. Ornelas-Paz, M. A. Salas-Marina, E. Villalobos-Pérez and V. M. Guerrero-Prieto, 2016. Identification and antagonistic activity in vitro of Bacillus spp. and Trichoderma spp. isolates against common phytopathogenic fungi. Rev. Mex. Fitopatol., 34: 84-99. https://cutt.ly/InRQHuE
Statistical Analysis System (SAS) Software, University Edition; Retrieved May 2, 2020, from. https://cutt.ly/tnRQLDq
Smith, D. L., Praslickova, D., & Ilangumaran, G. 2015. Inter-organismal signaling and management of the phytomicrobiome. Front Plant Sci, 6: 722. https://cutt.ly/CnRQCVT