Efecto de Alternaria alternata (Fr.) Keissl. sobre la fisiología de guisantes (Pisum sativum L.)

Palabras clave: estrés biótico, bioquímica, tizón, germinación, experimento en maceta


Alternaria alternata (Fr.) Keissl. es responsable de causar manchas foliares y enfermedades de tizón en una gran cantidad de cultivos hortícolas y agrícolas, incluido el guisante. La respuesta y resistencia de diferentes variedades de guisantes a este hongo puede variar. Por lo tanto, el objetivo de este estudio fue investigar las diferencias varietales en la respuesta fisiológica de Pisum sativum L. a A. alternata. Varios parámetros fisiológicos y bioquímicos de dos variedades comerciales de guisantes (Leina y Meature) fueron evaluadas en etapa vegetativa en macetas experimentales. Semillas estériles se humedecieron durante 15 min con una suspensión de 1×105 mL de conidios de A. alternata y las semillas de control se humedecieron con agua estéril. Estas semillas se sembraron en macetas que contenían tierra estéril. En ambas variedades el porcentaje de germinación, tasa de germinación, índice de vigor de plántulas, longitud de raíz y brote, biomasa seca y fresca, azúcar de hoja, contenido de clorofila a y b, contenido de clorofila total y de carotenoides, potencial osmótico foliar, contenido relativo de agua foliar, disminuyeron significativamente en A. alternata estresada en comparación con el control. Pero el contenido de prolina y proteínas aumentó significativamente. Se concluye de estos resultados que A. alternata causó efectos adversos en la fisiología de la planta de guisantes. Se encontró que la variedad de guisantes Leina es más sensible bajo el estrés fúngico de A. alternata en comparación con Meature y el grado de estrés causado por ambas variedades de prueba puede ser aprovechadas en estudios futuros.


La descarga de datos todavía no está disponible.


Abdul-Baki, A.A. and J.D. Anderson. 1973. Vigor determination in soybean seed by multiple criteria. Crop. Sci. 13(6):630-633.

Agrios, G.N. 2005. Plant Pathology. Fifth edition, Academic Press. New York. 978 0120445646N. 633 p.

Alqarawi, A.A., A. Hashem and E.F. Abd-Allah. 2013. Physiological alterations in Avicennia marina (Forski) Vierh. associated with leaf spot disease caused by Alternaria alternata (Fr.) Keissler. Pak. J. Bot. 45(5):1767-1771.

Aneja, J.K. and A. Agnihotri. 2013. Alternaria blight of oilseed brassicas: epidemiology and disease control strategies with special reference to use of biotechnological apporoaches for attaining host resistance. Journal Oilseed Brassica 4:1-10.

Arie, T., H. Takahashi, M. Kodama and T. Teraoka. 2007. Tomato as a model plant for plant-pathogen interactions. Plant. Biotechnol. 24(1):135-147.

Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant. Physiol. 24(1):1-15.

Bates, L.S., R.P. Waldren and I.D. Teare. 1973. Rapid determination of free proline for water-stress studies. Plant. Soil. 39(1):205-207.

Capell, B. and K. Dörffling. 1993. Genotype‐specific differences in chilling tolerance of maize in relation to chilling‐induced changes in water status and abscisic acid accumulation. Physiol. Plant. 88(4):638-646.

Claussen, W. 2005. Proline as a measure of stress in tomato plants. Plant. Sci. 168(1):241-248.

Dubois, M., K.A. Gilles, J.K. Hamilton, P.T. Rebers and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28(3):350-356.

El-Khallal, S.M. 2007. Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (arbuscular mycorrhiza) and/or hormonal elicitors (jasmonic acid and salicylic acid): 2-changes in the antioxidant enzymes, phenolic compounds and pathogen related-proteins. Aust. J. Basic. Appl. Sci. 1(4):717-732.

Food and Agriculture Organization of the United Nations 2018. Faostat. Retrieved of http://www.fao.org/faostat/en/#data/QC.

Fondevilla, S., C. M. Avila, J. I. Cubero. and D. Rubiales. 2005. Response to Mycosphaerella pinodes in a germplasm collection of Pisum spp. Plant Breeding, 124: 313–315

Gachande, B.D. and V.A. Jadhav. 2010. Effect of culture filtrates on seed germination and seedling growth of gram (Cicer arietinum L.). BIOINFOLET-A Quarterly Journal of Life Sci. 7(2): 173-174.

Hadi, H. W. 2019. Secondary metabolites importance in Alternaria alternata fungus. Pak. J. Biotechnol. 16(4):237-244.

Hadizadeh, I., B. Peivastegan and M. Kolahi. 2009. Antifungal activity of nettle (Urtica dioica L.), colocynth (Citrullus colocynthis L. Schrad), oleander (Nerium oleander L.) and konar (Ziziphus spina-christi L.) extracts on plants pathogenic fungi. Pak. J. Biol. Sci. 12(1):58-63.

Hiscox, J.T. and G.F. Israelstam. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57(12):1332-1334.

Houssien, A.A., S.M. Ahmed and A.A. Ismail. 2010. Activation of tomato plant defense response against Fusarium wilt disease using Trichoderma harzianum and salicylic acid under greenhouse conditions. Res. J. Agric. Biol. Sci. 6(3):328-338.

Khan, A., I.A. Nasir, B. Tabassum, K. Aaliya, M. Tariq, A.Q. Rao. 2017. Expression studies of chitinase gene intransgenic potato against Alternaria solani. Plant. Cell. Tissue. Organ. Cult. 128:563-576.

Lichtenthaler, H.K. and A.R. Wellburn. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 11:591-592.

Lin, Q., H. Su and H. He. 2014. First report of Alternaria alternata causing leaf spot on Bruguiera gymnorrhiza in China. Plant Dis. 99:286.

Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1):265-275.

Maia, G.C.M., C.O. Goshi, J.F. Vieira, R.O. Pierre, J.B. Maia, P.M.R. Júnior and M.S. de Abreu. 2012. Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioide. Coffee. Sci. 7:152-159.

Meena, P.D., A. Rani, R. Meena, P. Sharma, R. Gupta and P. Chowdappa. 2012. Aggressiveness, diversity and distribution of Alternaria brassicae isolates infecting oilseed Brassica in India. Afr. J. Microbiol. 6:5249-5258.

Mostafavi, K., H.S. Geive, M. Dadresan and M. Zarabi. 2011. Effects of drought stress on germination indices of corn hybrids (Zea mays L.). Int. J. Agri. Sci. 1(1):10-18.

Noelting, M.S., M. Sisterna, M. Lovisolo, A. Molla-Kralj, G. Lori, M.C. Sandoval, M. Sulyok and M.C. Molina. 2016. Discoloured seeds of amaranth plant infected by Alternaria alternata: physiological, histopathological alterations and fungal secondary metabolites associated or registered. J. Plant. Protect. Res. 56(3):244-249.

Orcutt, D.M. and E.T. Nilsen. 2000. Influence of plant phytopathogens on host physiology. The physiology of plants under stress. Soil and biotic factors. Wiley, USA p. 236-239.

Pangrikar, P.P., D.P. Gadgile, A.D. Hatti and A.M. Chavan. 2009. Seed physiology of different crops under the influence of bt and non-bt cotton rhizospheric fungi culture filtrate. J. Phytol. 1(6):404-407.

Perelló, A., M. Gruhlke and A.J. Slusarenko. 2013. Effect of garlic extract on seed germination, seedling health, and vigour of pathogen-infested wheat. J. Plant. Prot. Res. 53(4):317-323.

Rodino, S., M. Butu, P. Petrache, A. Butu and C.P. Cornea. 2014. Antifungal activity of four plants against Alternaria alternata. Scientific Bulletin. Series F. Biotechnologies 18:60-65.

Scharte, J., H. Schön and E. Weis. 2005. Photosynthesis and carbohydrate metabolism in tobacco leaves during an incompatible interaction with Phytophthora nicotianae. Plant. Cell. Environ. 28(11):1421-1435.

Sharma, M., G. Raju. and S, Pande. 2013. Occurrence of Alternaria alternata causing Alternaria blight in pigeonpea in India. Adv. Biosci. Biotechnol. 4(6): 702-705.

Shirurkar, D.D. and N.K. Wahegaonkar. 2012. Effect of aflatoxin on germination and seedling growth. Arch. Appl. Sci. Res. 4(6):2441-2446.

Tapwal, A., S. Garg, N. Gautam and R. Kuma. 2011. In vitro antifungal potency of plant extracts against five phytopathogens. Braz. Arch. Biol. Technol. 54(6):1093-1098.

Taj, G., P.D. Meena, P. Giri, D. Pandey, A. Kumar and A. Kumar. 2015. Pathogenesis mechanisms employed by Alternaria species. Journal Oilseed Brassica 6(2):213-240.

Tsuge, T., Y. Harimoto, K. Akimitsu, K. Ohtani, M. Kodama, Y. Akagi, M. Egusa, M. Yamamoto and H. Otani. 2013. Host-Selective toxins produced by the plant pathogenic fungus Alternaria alternata. FEMS Microbiol. Rev. 37: 44-66.

Wang, J., Y. Jiang, S. Chen, X. Xia, K. Shi, Y. Zhou and J. Yu 2010. The different responses of glutathione-dependent detoxification pathway to fungicide chlorothalonil and carbendazim in tomato leaves. Chemosphere 79(9):958-965.

Weatherley, P. 1950. Studies in the water relations of the cotton plant. New Phytol. 49(1):81-97.

Yang, W.X., F. Liu, N. Zhang, X.D. Ren and D.Q. Liu. 2013. First report of A. alternata causing blight on Zanthoxylum piperitum in China. Plant. Dis. 97:840.

Younas, A., K. Jabeen, S. Iqbal and S. Javed. 2016. Effect of Macrophomina phaseolina on germination, growth and physiology of Capsicum frutescens L. Pak. J. Phytopathol 28(02):207-211.

Cómo citar
Noureen, K., Jabeen, K., Iqbal, S., Jahan, S., & Javad, S. (2021). Efecto de Alternaria alternata (Fr.) Keissl. sobre la fisiología de guisantes (Pisum sativum L.). Revista De La Facultad De Agronomía De La Universidad Del Zulia, 38(3), 462-479. Recuperado a partir de https://produccioncientificaluz.org/index.php/agronomia/article/view/36175
Producción Vegetal. Crop Production. Produção Vegetal