Effect of the ruminal liquid extraction method in the in vitro gas technique

• Roselia Ramírez Díaz Estudiante de Doctorado en Ciencias Agropecuarias y Sustentabilidad, Universidad Autónoma de Chiapas https://orcid.org/0000-0002-6902-8955
• René Pinto Ruiz Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas, carretera Ocozocoautla-Villaflores km 84.5, Villaflores, Chiapas, México, C. P. 30470 https://orcid.org/0000-0003-1962-6874
• Francisco Guevara Hernández Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas, carretera Ocozocoautla-Villaflores km 84.5, Villaflores, Chiapas, México, C. P. 30470 https://orcid.org/0000-0002-1444-6324
• José Apolonio Venegas Venegas Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas, carretera Ocozocoautla-Villaflores km 84.5, Villaflores, Chiapas, México, C. P. 30470 https://orcid.org/0000-0002-3855-8355
• Mariela Beatriz Reyes Sosa Estudiante de Doctorado en Ciencias Agropecuarias y Sustentabilidad, Universidad Autónoma de Chiapas Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas, carretera Ocozocoautla-Villaflores km 84.5, Villaflores, Chiapas, México, C. P. 30470 https://orcid.org/0000-0002-9977-4246
• Luis Alberto Miranda Romero Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas, carretera Ocozocoautla-Villaflores km 84.5, Villaflores, Chiapas, México, C. P. 30470 https://orcid.org/0000-0003-0342-7897
• Luis Alberto Miranda Romero Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas, carretera Ocozocoautla-Villaflores km 84.5, Villaflores, Chiapas, México, C. P. 30470 https://orcid.org/0000-0003-0342-7897

Keywords: in vitro, sheep, degradation, fermentation

Abstract

In vitro gas studies require the use of cannulated animals; however, the high cost of the surgery limits its application. Therefore, the objective of the study was to evaluate the effect of the ruminal fluid extraction technique by oro-ruminal probe affects the results derived from the in vitro gas production technique (GPT). Elaeis guineensis meal was used as a substrate. Ruminal fluid was obtained in two ways: cannulated animals and oro-ruminal probe. Dry matter degradation was estimated (DMD), parameters of fermentation kinetics and fractional volume by the GPT. The comparison of means was carried out using the Tukey procedure (p<0,05). The results indicated the lack of significant statistical differences (p>0,05). It was concluded that the ruminal fluid extraction technique did not modify the DGRMS, the parameters and fermentation fractions of E. guineensis.

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