Influencia del bicarbonato de sodio sobre el crecimiento y producción de metabolitos de la cianobacteria Nostoc LAUN0015 / Influence of sodium bicarbonate on growth and metabolite production of the cyanobacterium Nostoc LAUN0015

• Luis López Laboratorio de Microorganismos Fotosintéticos, Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia.
• Carlos González Laboratorio de Microorganismos Fotosintéticos, Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia.
• Angélica Trompetera Laboratorio de Microorganismos Fotosintéticos, Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia.
• Luis Vargas Laboratorio de Microorganismos Fotosintéticos, Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia.
• Néstor Rosales Loaiza Laboratorio de Microorganismos Fotosintéticos, Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia.

Abstract

Resumen

El cultivo de cianobacterias permite el estudio de su fisiología y composición bioquímica. Para estudiar como el bicarbonato modula el crecimiento y producción de metabolitos de Nostoc LAUN0015, se estableció un ensayo a diferentes concentraciones (0; 2,5; 5 y 10 mM NaHCO₃), con medio BG-11₀ y bajo condiciones de laboratorio. A 5 mM NaHCO₃ se hallaron los máximos de clorofila a, carotenoides, ficocianina, ficoeritrina, proteínas y lípidos con 24,35±0,74; 5,52±1,25; 33,75±1,90; 46,91±5,45; 1420,95±53,05 y 36,11±0,74 µg·mL^-1, respectivamente. Se confirma el efecto positivo del bicarbonato sobre el crecimiento de Nostoc LAUN0015, lo cual puede ser utilizado como punto de partida en estudios posteriores sobre producción de biomasa.

Abstract

Culture of cyanobacteria allows the study of its physiology and biochemical composition. To assess how sodium bicarbonate modulates growth and metabolite production in Nostoc LAUN0015, a bioassay at different concentrations (0, 2.5, 5 and 10 mM NaHCO₃) with medium BG-11₀ and under laboratory conditions was established. To 5 mM NaHCO₃ were found higher values of chlorophyll a, carotenoids, phycocianin, phycoerithrin, protein and lipids with 24.35 ±0.74, 5.52 ±1.25, 33.75 ±1.90, 46.91 ±5.45, 1420.95 ±53.05 and 36.11 ±0.74 µg·mL^-1, respectively. It is confirmed the positive effect of bicarbonate on growth of Nostoc LAUN0015, which can be used as a starting point for further studies on production of biomass.

References

Beardall, J. y J. Raven. 2016. Carbon acquisition by microalgae. pp. 89-99. In: Borowitzka, M., J. Beardall, J. Raven (Eds.). The physiology of microalgae. Springer International Publishing, Suiza.

Bennet, A. y L. Bogorad. 1973. Complementary chromatic adaptation in a filamentous blue-green alga. J. Cell Biol. 58:419-435.

Devgoswami, C., M. Kalita, J. Talukdar, R. Bora y P. Sharma. 2011. Studies on the growth behavior of Chlorella, Haematococcus and Scenedesmus sp. in culture media with different concentrations of sodium bicarbonate and carbon dioxide gas. African J. Biotechnol. 10(61):13128-13138.

Elumalai, S., G. Jegan, G. Saravanan, T. Sangeetha, D. Roop Singh. 2014. Studies on growth and biochemical analysis of three microalgal strains on different molar concentration of sodium bicarbonate. Indian J. Appl. Res. 4:60-62.

Gantar, M. y Z. Svirčev. 2008. Microalgae and cyanobacteria: food for thought. J. Phycol. 44:260-268.

Gardner, R., K. Cooksey, F. Mus, R. Macur, K. Moll, E. Eustance, R. Carlson, R. Gerlach, M. Fields y B. Peyton. 2012. Use of sodium bicarbonate to stimulate triacylglycerol accumulation in the chlorophyte Scenedesmus sp. and the diatom Phaeodactylum tricornutum. J. Appl. Phycol. 24:1311-1320.

Kochert, G. 1978. Carbohydrate determination by the phenol-sulfuric acid method. pp. 95-97. In: Hellebust, J. y J. Craigie (Eds.). Handbook of Phycological Methods.Physiological and Biochemical Methods.Cambridge University Press. Cambridge, R.U.

Lowry, O., H. Rosenbrough, A. Farr y R. Randall. 1951. Protein measurement with the folin-phenol reagent. J. Biol. Biochem. 193:265-275.

Marker, A., E. Nusch, H. Rai y B. Riemann. 1980 The measurement of photosynthetic pigments in freshwater and standarization of methods: conclusions and recommendations. Arch. Hydrobiol. Egebn. Limnol. 14:91-106.

Marsh, J. y D. Weinstein. 1966. Simple charring method for determination lipids. J. Lipid Res. 7:574-576.

Matos, A., R. Feller, E. Siegel Moecke, J. de Oliveira, A. Furigo, R. Bianchini y E. Sant’Anna. 2015. Chemical characterization of six microalgae with potential utility for food application. J. Am. Oil Chem. Soc. 42(1):81-92.

Otero, A. y M. Vincenzini. 2004. Nostoc (Cyanophyceae) goes nude: extracellular polysaccharides serve as a sink for reducing power under unbalanced C/N metabolism. J. Phycol. 40: 74-81.

Pancha, I., K. Chokshi, T. Ghosh, C. Paliwal, R. Maurya y S. Mishra. 2015. Bicarbonate supplementation enhanced biofuel production potential as well as nutritional stress mitigation in the microalgae Scenedesmus sp. CCNM 1077. Biores. Technol. 193:315-323.

Rosales-Loaiza, N., C. Aiello-Mazzarri, L. Gómez, B. Arredondo and E. Morales. 2017. Nutritional quality of biomass from four strains of Nostoc and Anabaena grown in batch cultures. Int. Food Res. J. 24:2212-2219.

Shah, M., A. Kasprzak, Y. Liang and M. Daroch. 2016. Growth kinetics, phycocyanin and lipid accumulation of thermophilic cyanobacteria under bicarbonate and saline conditions. New Biotechnol. 33: 165-166.

Strickland, J. andT. Parsons. 1972. A practical handbook of seawater analysis. Fish. Res. Board Can. Bull. 167. 310 p.

White, D., A. Pagarette, P. Rooks and S. Ali. 2013. The effect of sodium bicarbonate supplementation on growth and biochemical composition of marine microalgae cultures. J. Appl. Phycol. 25:153-165.