Effect of Bothrops spp and Bothrocopias spp Venom on Colorectal Adenocarcinoma Cells
Abstract
Colorectal cancer has become a global health challenge due to its high prevalence and mortality rates. Current treatments lack therapeutic selectivity, affecting both healthy and malignant cells, and represent high costs for the healthcare system. For this reason, studies of substances with antitumor capacity have increased in recent years. Some research has demonstrated the presence of components with anticancer potential in viper venom. Therefore, this study evaluated the effect of the complete venom of Bothrocophias myersi, Bothrops rhombeatus, and Bothrops punctatus specimens, as well as two fractions of Bothrops asper venom, on SW480 and SW620 colorectal adenocarcinoma cell lines and HaCaT non-malignant cells. Electrophoretic characterization by SDS-PAGE was performed on each complete venom or fraction, and subsequently, cytotoxicity assays were conducted using Sulforodamine B, with exposure times of 24 and 48 hours. In general, all the venoms exhibit strong cytotoxic effects, except for the BaV fraction of B. asper venom. B. punctatus venom showed statistically significant differences in its cytotoxic effect between healthy and tumor cells, especially on the SW480 cell line (<p=0.00), so further research is proposed to profile its components and study its apparent selectivity on these tumor cells.
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