Evaluation of the Production of Plant Regulators by Bacillus paralicheniformis

• Zitlhaly Esmeralda Mancillas Betancourt Autonomous University of Tamaulipas https://orcid.org/0009-0008-9032-3095
• Teófilo Flores Santiago Autonomous University of Tamaulipas
• Isidro Palos Pizarro Autonomous University of Tamaulipas https://orcid.org/0000-0003-0124-531X
• Simón Josías Téllez Luis Autonomous University of Tamaulipas
• Xochitl Fabiola De La Rosa Reyna National Polytechnic Institute https://orcid.org/0000-0003-4517-9481
• José Luis Hernández Mendoza National Polytechnic Institute https://orcid.org/0000-0002-1233-0133
• Jesús Di Carlo Quiroz Velásquez National Polytechnic Institute https://orcid.org/0000-0002-6021-0427

DOI: https://doi.org/10.5281/zenodo.18189157

Keywords: Microorganism, Agriculture, Food security, Mexico

Abstract

Bacillus paralicheniformis is a bacterium from the Bacillus genus that has gained increasing attention in agricultural biotechnology due to its ability to produce bioactive compounds, offering an eco-friendly alternative to conventional chemical pesticides. This study aimed to evaluate its potential as a plant growth promoter in native crops from northern Tamaulipas, Mexico, and assess its environmental impact. A soil sample was obtained from the inventory of the Experimental Biotechnology Laboratory at the Center for Genomic Biotechnology (IPN), and microbial isolation was performed using Congo red selective medium. The resulting colonies exhibited rough surfaces, dye absorption, and firm texture, consistent with Bacillus morphology. Molecular identification was conducted by amplifying the 16S rRNA gene using universal primers 27F and 1492R, yielding a 1085 bp sequence corresponding to Bacillus paralicheniformis. HPLC analysis confirmed the synthesis of indole-3-acetic acid and anthranilic acid, both key plant growth regulators. These findings support the potential of B. paralicheniformis as a biofertilizer, enhancing plant development while reducing reliance on chemical inputs. Its application represents an innovative strategy for advancing sustainable, efficient, and ecosystem-friendly agriculture.

References

Cáceda Quiroz, César Julio, Maraza Choque, Gisela July, Chachaque Callo, Dina Mayumi, Fora Quispe, Gabriela de Lourdes, Farfan Pajuelo, Diana Galeska, & Carpio Mamani, Milena. (2024). Aislamiento y caracterización filogenética de bacterias nativas cultivables procedentes de minas abandonadas en Tacna, Perú. Biotecnia, 26, e2130. Epub 08 de noviembre de 2024. https://doi.org/10.18633/biotecnia.v26.2130

Corrales Ramírez MSc, L. C., Caycedo Lozano MSc, L., Gómez Méndez, M. A., Ramos Rojas, S. J., & Rodríguez Torres, J. N. (2017). Bacillus spp: una alternativa para la promoción vegetal por dos caminos enzimáticos. Revista Nova Publicación Científica En Ciencias Biomédicas, 15(27), 45-65. https://doi.org/10.22490/24629448.1958

Chen, X., Ding, Y., Yang, Y., Song, C., Wang, B., Yang, S., Guo, Y., & Gong, Z. (2021). Protein kinases in plant responses to drought, salt, and cold stress. Journal of Integrative Plant Biology, 63(1), 53–78. https://doi.org/10.1111/jipb.13061

Hernández-Mendoza, J. L., Quiroz-Velásquez, J. D., Moreno-Medina, V. R., & Mayek-Pérez, N. (2008). Biosíntesis de ácido antranílico y ácido indolacético apartir de triptófano en una cepa de Azospirillumbrasilense nativa de Tamaulipas, México. Avances en Investigación Agropecuaria, 12(1),57-66. https://www.redalyc.org/articulo.oa?id=83712105

National Center for Biotechnology Information (NCBI)[Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [1988] – [cited 2025 Nov 12]. Available from: https://www.ncbi.nlm.nih.gov/

Méndez Espinoza, Claudia, y Miguel Ángel Vallejo Reyna. 2019. «Mecanismos De Respuesta Al estrés abiótico: Hacia Una Perspectiva De Las Especies Forestales». Revista Mexicana De Ciencias Forestales 10 (56). México, ME. https://doi.org/10.29298/rmcf.v10i56.567

Rivera-Cruz, María del Carmen. 2011. Soportes orgánicos De Bacterias Promotoras De Crecimiento Vegetal Y La Sustentabilidad Del Suelo. Revista Terra Latinoamericana 29 (2):179-88. https://terralatinoamericana.org.mx/index.php/terra/article/view/1192.

Romero, Eliezer, José Hernández, Juan Gonzalez†, Sanjuana Hernández, Amanda Oliva, and Jesús Quiroz. 2025. Identification of Indole Acetic Acid Biosynthesis Pathways in Trichoderma Asperellum and Trichoderma Koningiopsis. Revista De La Facultad De Agronomía De La Universidad Del Zulia 42 (2), e244229. DOI: https://doi.org/10.47280/RevFacAgron(LUZ).v42.n2.XIII.

Sheela Chandra, Kazim Askari, Madhumita Kumari, Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere and its effects on plant growth, Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 2, 2018, Pages 581-586, ISSN 1687-157X, https://doi.org/10.1016/j.jgeb.2018.09.001.

Wang, L. T., Lee, F. L., Tai, C. J., & Kasai, H. (2007). Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. International Journal of Systematic and Evolutionary Microbiology, 57(Pt 8), 1846–1850. https://doi.org/10.1099/ijs.0.64685-0