Study of Time and Frequency Immersion in the in vitro Multiplication of Paulownia Elongata S.Y.Hu.
Abstract
Most of the commercial clones of paulownia are micropropagated and nowadays micropropagation techniques are being investigated to reduce the costs of vitroplants, such as cultivation in temporary immersion systems, which have proven to be highly efficient for the in vitro multiplication of many species. The time and frequency of immersion in temporary immersion systems are the main parameters that determine their efficiency, which is why in this research it was proposed to study the time and frequency in the in vitro multiplication of Paulownia elongata S.Y.Hu in Automated Temporary Immersion Vessels (RITA®). A factorial experiment was conducted to determine the frequency (every 6 and 8 h) and time (1 and 2 min) of immersion, with five replicates per treatment and 12 paulownia microshoots per replicate. After two subcultures of 30 days each, it was found that the combination of a frequency of immersion every 8 h and 1 min of immersion allowed obtaining the highest number of nodes (8.6) and multiplication coefficient (10.0). The highest values of number of shoots (2.26) were achieved with the frequency every 6 h, independently of the immersion time and the length of the shoots was not affected by the variables studied. It is concluded that time and frequency of immersion were determinant in increasing in vitro multiplication of P. elongata in RITA®.
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References
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