Identification of Genes Homologous to Protease Inhibitors Present in the Genome of the Pathogenic Oomycete of the Solanaceae Family

• Estefanía Ramírez-Delgado Monterrey Institute of Technology, Mexico
• Verónica Ancona Texas A&M University https://orcid.org/0000-0001-8860-0382
• Gustavo A. Frías-Treviño Antonio Narro Autonomous Agrarian University, Mexico
• José Luis Hernández-Mendoza National Polytechnic Institute, Mexico https://orcid.org/0000-0002-8686-1340
• Israel García-León National Polytechnic Institute, Mexico https://orcid.org/0000-0002-7876-1219
• Jesús Di Carlo Quiroz-Velásquez National Polytechnic Institute, Mexico https://orcid.org/0000-0002-6021-0427

DOI: https://doi.org/10.5281/zenodo.14600156

Keywords: Biotechnology, Genes, Evolution, Computer programming, Enzymes

Abstract

The sequencing of genomes of various pathogens of the Phytophtora genus has led to the identification of genes encoding effector proteins that allow evasion and manipulation of plant defense. The objective of the research was the search and identification of sequences homologous to protease and glucanase inhibitor proteins within the genome of P. capsici (CPV-282) isolated from chili in Mexico. The methodology consisted of analyzing extracellular protein inhibitors (EPI), protease inhibitors with cystatin-like domains (EPIC) and glucanase inhibitor proteins (GIP) reported in Phytophtora infestans, Phytophtora sojae, Phytophtora ramorum and Phytophtora brassicae. Results obtained: The in silico analysis revealed the presence of homologous genes to EPI10, EPIC3, PiGIP and PsGIP, from which oligonucleotides were designed to confirm the prediction of the bioinformatics analysis. The sequences obtained from the products amplified by Polymerase Chain Reaction allowed the identification of sequences homologous to cystatin-type protease inhibitors (PcEPIC3) and glucanase inhibitors (PcGIP) within the P. capsici genome. The results suggest that the Phytophthora genus shares genetic tools that allow it to manipulate the plant's defense systems and thus be efficient in the colonization of its host.

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