The effect of hypothermia on the state of the prooxidant-antioxidant system of plants

Mariia  Bobrova; Olena  Holodaieva; Svitlana  Koval; Olha  Tsviakh; Olena  Kucher

doi:10.46925//rdluz.33.07

Mariia Bobrova Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University, Ukraine http://orcid.org/0000-0001-7703-651X
Olena Holodaieva Department of General and Biological chemistry #2 Donetsk National Medical University, Ukraine http://orcid.org/0000-0002-4922-7033
Svitlana Koval Department of the Fundamental Disciplines of the International European University, Ukraine http://orcid.org/0000-0002-4907-177X
Olha Tsviakh Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University, Ukraine http://orcid.org/0000-0002-1119-2170
Olena Kucher Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University, Ukraine. http://orcid.org/0000-0002-9963-6855

DOI: https://doi.org/10.46925//rdluz.33.07

Keywords: biochemistry; metabolism; enzymes; vitamins; cooling, freezing

Abstract

Aim of the research: to identify changes in the value of indicators of the state of the prooxidant-antioxidant system (PAS) in the tissues of edible parts of agricultural plants under the influence of temperature changes. Methodology: Quantitative determination of indicators of the state of PAS was performed on tissue samples of edible parts of the following plants: Solánum lycopérsicum L., Сucumis sativus L., Capsicum annuum L., Solanum melongena L., Solanum tuberosum L., Allium sativum L., Allium cepa L., Daucus carota L., Beta vulgaris L., Cucurbita pepo var. Giraumontia L. The concentration of superoxide anion radical (•O₂^-), TBA-active products, superoxide dismutase (SOD) activity, catalase, the concentration of ascorbic acid (AA), glutathione (GSH) were determined. The results of the research show that hypothermia activates both parts of the PAS, however, cooling is accompanied by more powerful both low molecular weight and enzymatic antioxidant (AO) protection. The research of AO can be divided according to the degree of reduction of the protective value in hypothermia in the following series: SOD, catalase, GSH, AA. The most resistant in terms of changes in PAS to hypothermia is Solanum tuberosum L., Allium sativum L., Beta vulgaris L.; the least resistant is Capsicum annuum L., and Solánum lycopérsicum L. The generative organs of plants are less resistant to hypothermia than the vegetative ones. Practical consequences. As a result of the conducted biochemical analysis, it is established which method of storage of plant products is more effective in terms of preservation of AO activity: cooling or freezing.

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Author Biographies

Mariia Bobrova , Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University, Ukraine

Senior Lecturer of the Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University, Ukraine

Olena Holodaieva , Department of General and Biological chemistry #2 Donetsk National Medical University, Ukraine

Associate Professor of the Department of General and Biological chemistry #2 Donetsk National Medical University, Ukraine

Svitlana Koval , Department of the Fundamental Disciplines of the International European University, Ukraine

Senior Lecturer of the Department of the Fundamental Disciplines of the International European University, Ukraine

Olha Tsviakh , Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University, Ukraine

Senior Lecturer of the Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University, Ukraine

Olena Kucher , Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University, Ukraine.

Senior Lecturer of the Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University, Ukraine.

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