The value of the prooxidant-antioxidant system in ensuring the immunity of plants

Mariia Bobrova; Olena Holodaieva; Hanna Arkushyna; Olena Larycheva; Olha Tsviakh

Mariia Bobrova Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University http://orcid.org/0000-0001-7703-651X
Olena Holodaieva Department of General and Biological chemistry #2 Donetsk National Medical University http://orcid.org/0000-0002-4922-7033
Hanna Arkushyna Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University http://orcid.org/0000-0002-5261-7315
Olena Larycheva Department of Pharmacy, Pharmacology, Medical, Bioorganic and Biological Chemistry of the Petro Mohyla Black Sea National University http://orcid.org/0000-0001-7399-3339
Olha Tsviakh Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University http://orcid.org/0000-0002-1119-2170

Keywords: ascorbic acid, catalase, superoxide anion radical, superoxide dismutase, cytochrome oxidase

Abstract

Aim of the research: to identify changes in the state of the prooxidant-antioxidant system in the organs of different plant varieties, depending on their level of resistance to disease. The subject of the research is the role of individual components of the prooxidant-antioxidant system in ensuring plant resistance to disease. Methodology. Quantitative determination of PAS status was performed on onion tissue samples taken from the following varieties: "Globus" (high-resistant variety), "Rainbow" (medium-resistant variety) and "Donetsk Golden" ( low-resistant variety). For biochemical analysis, tissues from the top of the leaf, the middle of the leaf, the scales of the onion-turnip, stem, flower, roots, and seeds were used. The concentration of superoxide anion radical, TBA-active products, superoxide dismutase activity, catalase, GSH-peroxidase, the concentration of ascorbic acid, glutathione, cytochrome oxidase activity were determined. The results of the research show that іn the tissues of photosynthetic vegetative organs of onions, there is an increase in both parts of the prooxidant-antioxidant system; in tissues that are not capable of photoproduction, there is an advantage of the antioxidant link in accordance with the increased resistance of the variety to disease. Initiation of germination processes enhances the activity of both parts of the prooxidant-antioxidant system and is highest in flower cells. Onion seed tissues, which are at rest, have the advantage of a prooxidant link and an increase in the concentration of low molecular weight antioxidants. Practical consequences. As a result of the correlation analysis of the studied indicators, the presence of a close relationship between the concentration of TBA-active products and the activity of cytochrome oxidase and superoxide dismutase, ascorbate with glutathione was established.

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Author Biographies

Mariia Bobrova, Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University

PhD in Biology, Senior Lecturer of the Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University

Olena Holodaieva, Department of General and Biological chemistry #2 Donetsk National Medical University

PhD in Chemistry, Associate Professor of the Department of General and Biological chemistry #2 Donetsk National Medical University

Hanna Arkushyna, Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University

PhD in Biology, Associate Professor of the Department of Biology and Methods of Teaching of the Volodymyr Vynnychenko Central Ukrainian State Pedagogical University

Olena Larycheva, Department of Pharmacy, Pharmacology, Medical, Bioorganic and Biological Chemistry of the Petro Mohyla Black Sea National University

PhD in Biology, Associate Professor of the Department of Pharmacy, Pharmacology, Medical, Bioorganic and Biological Chemistry of the Petro Mohyla Black Sea National University

Olha Tsviakh, Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University

PhD in Biology, Associate Professor of the Department of Chemistry of the V.O. Sukhomlynskyi Mykolaiv National University

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