Glyoxalase I (GLX-I) analysis in native maize from Oaxaca, Mexico, infected with Aspergillus flavus in vitro
Keywords:
GLX-I, stress, proteins, Aspergillus
Abstract
The glyoxalase system plays an important role in various physiological processes in plants when they are subjected to different types of stress, whether physical, chemical or biological. Aspergillus flavus is an aflatoxin-producing fungus that contaminates dry grains, leading to a gradual deterioration of the grains and a significant reduction in their nutritional value. The objective of the present study was to evaluate the activity of the enzyme glyoxalase I (GLX-I) in maize coleoptiles from Oaxaca in response to infection caused by Aspergillus flavus. Nine maize samples from four different races were analyzed. The samples were inoculated with a suspension of Aspergillus flavus spores of known concentration and total protein extraction and quantification were performed on the coleoptiles, and GLX-I activity was determined by quantifying the amount of S-lactoylglutathione produced per minute. In addition, analysis of gene expression by reverse transcriptase polymerase chain reaction (RT-PCR) was performed. The inoculated maize coleoptiles showed symptoms of infection, color changes and wilting. The concentration of total proteins decreased significantly in the extracts of four samples in the presence of the fungus. In the GLX-I analysis, two samples had the highest enzymatic activity in the infected coleoptile extract with respect to the healthy one, in addition to presenting greater expression of the gene in the RT-PCR assay, this due to the response to Aspergillus flavus infection.
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References
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Hasanuzzaman M., Nahar K., Hossain Md. S., Anee T. I., Parvin K. & Fujita M. (2017b). Nitric oxide pretreatment enhances antioxidant defense and glyoxalase systems to confer PEG-induced oxidative stress in rapeseed. Journal of Plant Interaction, 12(1): 323-331. https://doi.org/10.1080/17429145.2017.1362052
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Li Z-G. (2016). Methylglyoxal and glyoxalase system in plants: old players, new concepts. The Botanical Review, 82: 183-203. https://doi.org/10.1007/s12229-016-9167-9
Rosado O. A. and Villasante S. B. (2021). Los herederos del maíz. Instituto Nacional de los Pueblos Indígenas, Ciudad de México. https://www.gob.mx/cms/uploads/attachment/file/658352/Libro-Los-herederos-del-maiz-INPI.pdf
Martínez P. H. Y., Hernández S., Reyes C. A. & Vázquez-Carrillo G. (2013). El género Aspergillus y sus micotoxinas de maíz en México: problemática y perspectivas. Revista Mexicana de Fitopatología, 31(2): 126-146. http://www.scielo.org.mx/pdf/rmfi/v31n2/v31n2a5.pdf
Orozco-Ramírez Q., Ross-Ibarra J., Santacruz-Varela A. & Brush S. (2016). Maize diversity associated with social origin and environmental variation in Southern Mexico. Heredity, 116(5): 477-484. https://doi.org/10.1038/hdy.2016.10
Rajasekaran K., Sayler R. J., Majumdar R., Sickler C. M. & Cary J. W. (2019). Inhibition of Aspergillus flavus growth and aflatoxin production in transgenic maize expressing the α-amylase inhibitor from Lablab purpureus L. Journal of Visualized Experiments, 144. https://doi.org/10.3791/59169
Rohman Md. M., Talukder M. Z. A., Hossain M. G., Uddin M. S., Amiruzzaman M., Biswas A., Ahsan A. F. M. & Chowdhury M. A. Z. (2016). Saline sensitivity leads to oxidative stress and increases the antioxidants in presence of proline and betaine in maize (Zea mays L.) inbred. Plants Omics Journal, 9(1): 35-47. omics.com/rohman_9_1_2016-35_47.pdf
Sadiq I., Fanucchi F., Paparelli E., Alpi E., Bachi A., Alpi A. & Perata P. (2011). Proteomic identification of differentially expressed proteins in the anoxic rice coleoptile. Journal of Plant Physiology, 168:2234-2243. https://doi.org/10.1016/j.jplph.2011.07.009
Servicio de Información Agroalimentaria y Pesquera. SIAP. (2021). Anuario estadístico de la producción agrícola. Consultado el 9 de mayo de 2022. https://nube.siap.gob.mx/cierreagricola/
Schneider C., Rasband W. & Eliceiri, K. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9 (7): 671–675. https://doi.org/10.1038/nmeth.2089
Turra G. L., Agostini R. B., Fauguel C. M., Presello D. A., Andreo C. S., González J. M. & Campos-Bermudez V. A. (2015). Structure of the novel monomeric glyoxalase I from Zea mays. Acta Crystallogr D Biol Crystallogr, 71(10): 2009-2020.
https://doi.org/10.1107/S1399004715015205
Zhou Z-H, Wang Y., Ye X-Y & Li Z-G. (2018). Signaling molecule hydrogen sulfide improves seed germination and seedling growth of Maize (Zea mays L.) under high temperature by inducing antioxidant system and osmolyte biosynthesis. Frontiers in Plant Science, 9: 1288. https://doi.org/10.3389/fpls.2018.01288
Varapizuela S. C. F., Sánchez M. M. A. & Pérez S. A. D. (2019). Análisis de la actividad enzimática de peroxidasas en maíces nativos de Oaxaca infectados por Aspergillus parasiticus. Revista Ingeniantes, 2(2): 75-79. https://citt.itsm.edu.mx/ingeniantes/pdfversion/ingeniantes6no2vol2-esp.pdf
Aragón C. F., Taba S, Hernández J. M., Figueroa J. D. & Serrano V. (2006). Actualización de la información sobre los maíces criollos de Oaxaca. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Informe final SNIB-CONABIO proyecto No. CS002 México D. F. http://www.conabio.gob.mx/institucion/proyectos/resultados/InfCS002.pdf
Arteaga M. C., Moreno-Letelier A., Mastretta-Yanes A., Vázquez-Lobo A., Breña-Ochoa A., Moreno-Estrada A., Eguiarte L. E. & Piñero D. (2016). Genomic variation in recently collected maize landraces from Mexico. Genomics Data, 7: 38-45. https://doi.org/10.1016/j.gdata.2015.11.002
Bandyopadhyay R., Ortega-Beltrán A., Akande A., Mutegi C., Atehnkeng J., Kaptoge L., Senghor A. L., Adhikari B. N. & Cotty P. J. (2016). Biological control of aflatoxins in Africa: current status and potential challenges in the face of climate change. World Mycitoxin Journal, 9(5): 771-789. https://doi.org/10.3920/WMJ2016.2130
Bania I. and Mahanta R. (2012). Evaluation of peroxidases from various plant sources. International Journal of Scientific and Research Publications, 2(5). http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.387.3615&rep=rep1&type=pdf
Baker R. L., Brown R. L., Chen Z. Y., Cleveland T. E. & Fakhoury A. M. (2009). A maize lectin-like protein with antifungal activity against Aspergillus flavus. Journal of food protection, 72(1): 120-127. https://doi.org/10.4315/0362-028X-72.1.120
Ben M. R., El-Omari R., El-Mourabit N., Bouargalne Y. & NHIRI M. (2018). Changes in the antioxidant and glyoxalase enzyme activities in leaves of two Moroccan sorghum ecotypes with differential tolerance to nitrogen stress. Australian Journal of Crop Science, 12(8): 1280-1287. https://www.cropj.com/mrid_12_8_2018_1280_1287.pdf
Borysiuk K., Ostaszewska-Bugajska M., Vaultier M-N, Hasenfratz-Sauder M-P & Szal B. (2018). Enhanced formation of methylglyoxal-derived advanced glycation end products in Arabidopsis under Ammonium Nutrition. Frontiers in Plant Science, 9: 667. https://doi.org/10.3389/fpls.2018.00667
Cabrera T. J. M., Carballo A. & Aragón-Cuevas F. (2015). Evaluación agronómica de maíces raza Zapalote chico en la región Istmeña de Oaxaca. Revista Mexicana Ciencias Agrícolas, 11: 2075-2082. https://doi.org/10.29312/remexca.v0i11.775
Chen Z. Y., Brown R. L., Damann K. E. & Cleveland T. E. (2004). Identification of a maize kernel stress-related protein and its effect on aflatoxin accumulation. Phytopathology, 94(9): 938-945. https://doi.org/10.1094/PHYTO.2004.94.9.938
Chen, Z.-Y., Brown R. L., Damann K. E. & Cleveland T. E. (2007). Identification of maize kernel endosperm proteins associated with resistance to aflatoxin contamination by Aspergillus flavus. Phytopathology, 97(9): 1094–1103. https://doi.org/10.1094/PHYTO-97-9-1094
Chomczynski P. and Sacchi N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162(1):156-9. https://doi.org/10.1016/0003-2697(87)90021-2
Fernández S. R., Morales L. A. & A. Gálvez M. (2013). Importancia de los maíces nativos de México en la dieta nacional. Una revisión indispensable. Revista Fitotecnia Mexicana, 36(3-A): 275-283. http://www.scielo.org.mx/pdf/rfm/v36s3-a/v36s3-aa4.pdf
Fountain J. C., Chen Z-Y, Scully B. T., Kamerait R. C., Lee R. D. & Guo B. (2010). Pathogenesis-related gene expressions in different maize genotypes under drought stressed conditions. African Journal Plant Science, 4(11): 433-440. https://academicjournals.org/article/article1380126077_Fountain%20et%20al.pdf
Ghosh A. (2017). Genome-wide identification of glyoxalase genes in Medicago truncatula and their expression profiling in response to various developmental and environmental stimuli. Frontiers in Plant Science, 8: 836. https://doi.org/10.3389/fpls.2017.00836
Ghosh A., Kushwana H. R., Hasan M. R., Pareek A., Sopory S. K. & Singla-Pareek S. L. (2016). Presence of unique glyoxalase III proteins in plants indicates the existence of shorter route for methylglyoxal detoxification. Scientific Reports, 6:18358. https://doi.org/10.1038/srep18358
Hasanuzzaman M., Nahar K., Hossain Md. S., Mahmud J. A., Rahman A., Inafuku M., Oku H. & Fujita M. (2017a). Coordinated actions of glyoxalase and antioxidant defense systems in conferring abiotic stress tolerance in plants. International Journal Molecular Sciences, 18(1): 200. https://doi.org/10.3390/ijms18010200
Hasanuzzaman M., Nahar K., Hossain Md. S., Anee T. I., Parvin K. & Fujita M. (2017b). Nitric oxide pretreatment enhances antioxidant defense and glyoxalase systems to confer PEG-induced oxidative stress in rapeseed. Journal of Plant Interaction, 12(1): 323-331. https://doi.org/10.1080/17429145.2017.1362052
Kato Y. T. A., Mapes C., Mera L. M., Serratos J. A. & Bye R. A. (2009). Origen y diversificación del maíz; Una revisión analítica. UNAM: México: 115 pp. https://issuu.com/perrasdelmal/docs/origen_y_diversif._del_maiz
Kaur C., Sharma S., Hasan M. R., Pareek A., Singla-Pareek S. L. & Sopory S. K. (2017). Characteristic variations and similarities in biochemical, molecular and functional Properties of glyoxalases across Prokaryotes and Eukaryotes. International Journal of Molecular Sciences, 18:250. https://doi.org/10.3390/ijms18040250
Li Z-G. (2016). Methylglyoxal and glyoxalase system in plants: old players, new concepts. The Botanical Review, 82: 183-203. https://doi.org/10.1007/s12229-016-9167-9
Rosado O. A. and Villasante S. B. (2021). Los herederos del maíz. Instituto Nacional de los Pueblos Indígenas, Ciudad de México. https://www.gob.mx/cms/uploads/attachment/file/658352/Libro-Los-herederos-del-maiz-INPI.pdf
Martínez P. H. Y., Hernández S., Reyes C. A. & Vázquez-Carrillo G. (2013). El género Aspergillus y sus micotoxinas de maíz en México: problemática y perspectivas. Revista Mexicana de Fitopatología, 31(2): 126-146. http://www.scielo.org.mx/pdf/rmfi/v31n2/v31n2a5.pdf
Orozco-Ramírez Q., Ross-Ibarra J., Santacruz-Varela A. & Brush S. (2016). Maize diversity associated with social origin and environmental variation in Southern Mexico. Heredity, 116(5): 477-484. https://doi.org/10.1038/hdy.2016.10
Rajasekaran K., Sayler R. J., Majumdar R., Sickler C. M. & Cary J. W. (2019). Inhibition of Aspergillus flavus growth and aflatoxin production in transgenic maize expressing the α-amylase inhibitor from Lablab purpureus L. Journal of Visualized Experiments, 144. https://doi.org/10.3791/59169
Rohman Md. M., Talukder M. Z. A., Hossain M. G., Uddin M. S., Amiruzzaman M., Biswas A., Ahsan A. F. M. & Chowdhury M. A. Z. (2016). Saline sensitivity leads to oxidative stress and increases the antioxidants in presence of proline and betaine in maize (Zea mays L.) inbred. Plants Omics Journal, 9(1): 35-47. omics.com/rohman_9_1_2016-35_47.pdf
Sadiq I., Fanucchi F., Paparelli E., Alpi E., Bachi A., Alpi A. & Perata P. (2011). Proteomic identification of differentially expressed proteins in the anoxic rice coleoptile. Journal of Plant Physiology, 168:2234-2243. https://doi.org/10.1016/j.jplph.2011.07.009
Servicio de Información Agroalimentaria y Pesquera. SIAP. (2021). Anuario estadístico de la producción agrícola. Consultado el 9 de mayo de 2022. https://nube.siap.gob.mx/cierreagricola/
Schneider C., Rasband W. & Eliceiri, K. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9 (7): 671–675. https://doi.org/10.1038/nmeth.2089
Turra G. L., Agostini R. B., Fauguel C. M., Presello D. A., Andreo C. S., González J. M. & Campos-Bermudez V. A. (2015). Structure of the novel monomeric glyoxalase I from Zea mays. Acta Crystallogr D Biol Crystallogr, 71(10): 2009-2020.
https://doi.org/10.1107/S1399004715015205
Zhou Z-H, Wang Y., Ye X-Y & Li Z-G. (2018). Signaling molecule hydrogen sulfide improves seed germination and seedling growth of Maize (Zea mays L.) under high temperature by inducing antioxidant system and osmolyte biosynthesis. Frontiers in Plant Science, 9: 1288. https://doi.org/10.3389/fpls.2018.01288
Varapizuela S. C. F., Sánchez M. M. A. & Pérez S. A. D. (2019). Análisis de la actividad enzimática de peroxidasas en maíces nativos de Oaxaca infectados por Aspergillus parasiticus. Revista Ingeniantes, 2(2): 75-79. https://citt.itsm.edu.mx/ingeniantes/pdfversion/ingeniantes6no2vol2-esp.pdf
Published
2022-09-21
How to Cite
Varapizuela-Sánchez, C. F., Sánchez-Medina, M. A., Pina-Canseco, M. del S., Rosas-Murrieta, N. H., Pérez-Santiago, A. D., & García-Montalvo, I. A. (2022). Glyoxalase I (GLX-I) analysis in native maize from Oaxaca, Mexico, infected with Aspergillus flavus in vitro. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 39(4), e223946. Retrieved from https://produccioncientificaluz.org/index.php/agronomia/article/view/38769
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Crop Production
Copyright (c) 2022 Carlos Francisco Varapizuela-Sánchez, Marco Antonio Sánchez-Medina, María del Socorro Pina-Canseco, Nora Hilda Rosas-Murrieta, Alma Dolores Pérez-Santiago, Iván Antonio García-Montalvo
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