Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients
Abstract
The resistance to methicillin and macrolides in Staphylococcus aureus endangers the efficacy of antibiotic treatment, thus becoming a public health problem; the objective of this study was to characterize from the molecular point of view strains of Staphylococcus aureus resistant to methicillin and isolated from hospitalized patients. This was a descriptive and cross-sectional study, the identification and determination of antimicrobial susceptibility was made by automated methods on the BioMérieux 2 Vitek Compact equipment, phenotypic screening for the detection of methicillin resistance and D-test was carried out according to the standardized norms of the manual M100 S20 of the Clinical and Laboratory Standards Institute, genotyping was carried out by conventional polymerase chain reaction. The following results were obtained, 57 strains were isolated of which 50.88% (n = 29) were resistant to methicillin and of these same strains 27.6% (n = 8) presented inducible resistance to clindamycin. The prevalent resistance genes were mecA (100%) for methicillin and ermC (87.5%) for clindamycin. In the strains that presented multiresistance, the treatment would be limited to other families of antibiotics, increasing the toxicity and the
cost of the treatment.
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