Identification, characterization, and purification of xylanase PC-01 isolated from Pacet hot spring

• Mamik Damayanti, Ni Nyoman Purwani Research Center for Bio-Molecule Engineering, Universitas Airlangga, Kampus C-UNAIR, Mulyorejo, Surabaya, 60115, Indonesia
• Ni Made Dwi Purwadi Departement of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Kampus C-UNAIR, Mulyorejo, Surabaya, 60115, Indonesia
• Lailatul Fithri Research Center for Bio-Molecule Engineering, Universitas Airlangga, Kampus C-UNAIR, Mulyorejo, Surabaya, 60115, Indonesia

Palabras clave: Endo-Β-Xylanase, Hydrophobic, Interactions, Chromatography

Resumen

This research aims to characterize, identify and purify the xylanolytic enzymes by hydrophobic interaction and anion exchange chromatography via the endo-β-xylanase activity test with the DNS method. The best results obtained from anion-exchange chromatography of third fraction pH 5 with the purity level of 78.25 times higher than crude extract. In conclusion, Characteristics of bacterial isolates PC-01 are rod-shaped bacterial cells, Gram-positive, and have subterminal oval endospores.