Microorganisms isolated from patients with COVID-19
Abstract
This study aims to identify microorganisms (bacteria and/ or yeasts) isolated from samples from patients held in the Intensive Care Unit of a Sentinel Hospital in the city of Maracaibo, with a confirmed diagnosis of COVID-19, during the period between January 1, 2020 and May 31, 2021. The antimicrobial susceptibility of the isolated microorganisms was also evaluated. The average age of the patients was 54.42 years, with a predominance of males (56.00%) and adults (66.00%). 41.75% (89/206) of the cultures were positive. In 72% (36/50) of patients, mixed cultures with bacteria and yeast were obtained. In bacteria, the observed resistance rate to all antibiotics was high (> 50.00%). All strains were multi-resistant. Antifungal resistance was variable according to the drug used, voriconazole (8.57%), fluconazole (3.57%); whereas, for caspofungin, total sensitivity (100.00%) was observed. The most frequent microorganisms were: Candida albicans Complex (17.45%), Acinetobacter baumannii/calcoaceticus Complex (15.30%) and Klebsiella pneumoniae (13.96%), results that show the predominance of these opportunistic pathogens as possible causes of colonization and / or co-infections in patients affected by COVID-19; as well as its manifest resistance to multiple antimicrobials
References
Vargas-Ponce KG, Salas-López JA, Llanos-Tejada FK, Morales-Avalos A. Coinfección de Covid-19 e influenza: Reporte de cinco casos en un hospital peruano. Rev Fac Med Humana [Internet]. 2020;20(4):738-42. Disponible en: http://revistas.urp.edu.pe/index.php/RFMH/article/view/3158 DOI: 10.25176/RFMH.v20i4.3158
Aguilera Calzadilla Y, Diaz Morales Y, Ortiz Díaz LA, Gonzalez Martínez OL, Lovelle Enríquez OA, Sánchez Álvarez M de L. Infecciones bacterianas asociadas a la COVID-19 en pacientes de una unidad de cuidados intensivos. Rev Cuba Med Mil [Internet]. 2020;49(3). Disponible en: https://revmedmilitar.sld.cu/index.php/mil/article/view/793/539
Xing Q, Li G, Xing Y, Chen T, Li W, Ni W, et al. Precautions are Needed for COVID-19 Patients with Coinfection of Common Respiratory Pathogens. medRxiv [Internet]. 2020;2020.02.29.20027698. Disponible en: http://medrxiv.org/content/early/2020/03/05/2020.02.29.20027698.abstract DOI: 10.1101/2020.02.29.20027698
Wu X, Cai Y, Huang X, Yu X, Zhao L, Wang F, et al. Co-infection with SARS-CoV-2 and Influenza A Virus in Patient with Pneumonia, China. Emerg Infect Dis J [Internet]. 2020;26(6):1324. Disponible en: https://wwwnc.cdc.gov/eid/article/26/6/20-0299_article DOI: 10.3201/eid2606.200299 PMID 32160148 PMCID PMC7258479
Zhou P, Yang X-L, Wang X-G, Hu B, Zhang L, Zhang W, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature [Internet]. 2020;579(7798):270-3. Disponible en: https://www.nature.com/articles/s41586-020-2012-7 DOI: 10.1038/s41586-020-2012-7 PMID 32015507 PMCID PMC7095418
Kim D, Quinn J, Pinsky B, Shah NH, Brown I. Rates of Co-infection Between SARS-CoV-2 and Other Respiratory Pathogens. JAMA [Internet]. 2020;323(20):2085-6. Disponible en: https://doi.org/10.1001/jama.2020.6266 DOI: 10.1001/jama.2020.6266 PMID 32293646 PMCID PMC7160748
Agencia española de medicamentos y productos. Resistencia bacteriana y COVID-19: recomendaciones del PRAN para el uso prudente de los antibióticos durante la pandemia - Agencia Española de Medicamentos y Productos Sanitarios [Internet]. Ministerio de sanidad. 2020. Disponible en: https://www.aemps.gob.es/informa/notasinformativas/laaemps/2020-laaemps/resistencia-bacteriana-y-covid-19-recomendaciones-del-pran-para-el-uso-prudente-de-los-antibioticos-durante-la-pandemia/#
Hernández Sampieri R, Fernández Collado C, Baptista Lucio P. “Metodología de la Investigación”. Sexta Edición. McGraw-Hill/Interamericana Editores, S.A. de C.V. México, D.F. (2014). 634 p.
Arias F. 2006. “El Proyecto de Investigación. Introducción a la Metodología Científica”. Quinta edición. Editorial Episteme. Caracas, Venezuela.p21-24.
García L. 2010. “Curso de Introducción a la Metodología de la Investigación, Tipos de Diseños de Ia Investigación”. Disponible en: http://www.cochrane.es/files/Recursos/presentacio_LMartinez.pdf
CLSI. Performance standards for Antimicrobial susceptibility testing. 31th ed. CLSI Supplement M100. Wayne PA: Clinical and Laboratory Clinical Institute. 2021. 352 pp.
CLSI. 2020. Document M60. Performance Standards for Antifungal Susceptibility Testing of Yeasts. Second edition. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA.13 pp.
The World Medical Association. Declaración de Helsinki de la AMM - Principios éticos para las investigaciones médicas en seres humanos. 2015. Disponible en: https://www.wma.net/es/policies-post/declaracion-de-helsinki-de-la-amm-principios-eticos-para-las-investigaciones-medicas-en-seres-humanos/
Peña García Y, Domínguez Fernández BN, Gómez Cook K, Garrido González D, Labrada Solorzano AM. Caracterización clínico epidemiológica de pacientes sospechosos y positivos a la COVID-19 en Puerto Padre. MEDISAN [Internet]. 2020;24(5). Disponible en: https://medisan.sld.cu/index.php/san/article/view/3177/2077
Garibaldi BT, Fiksel J, Muschelli J, Robinson ML, Rouhizadeh M, Perin J, et al. Patient Trajectories Among Persons Hospitalized for COVID-19. Ann Intern Med [Internet]. 2020;174(1):33-41. Disponible en: https://doi.org/10.7326/M20-3905 DOI: 10.7326/M20-3905 PMID 32960645 PMCID PMC7530643
Roblejo Balbuena H, Benítez Cordero Y, Álvarez Gavilán Y, Bravo Ramírez M, Pereira Roche N, García Gómez D, et al. Características clínico-epidemiológicas de pacientes cubanos residentes en La Habana afectados por la COVID-19. Rev Cuba Investig Biomédicas [Internet]. 2021;40(2). Disponible en: https://revibiomedica.sld.cu/index.php/ibi/article/view/1566
Ruiz Cantero MT. Las estadísticas sanitarias y la invisibilidad por sexo y de género durante la epidemia de COVID-19. Gac Sanit [Internet]. 2021;35(1):95-8. Disponible en: https://www.sciencedirect.com/science/article/pii/S0213911120300911?via%3Dihub DOI: 10.1016/j.gaceta.2020.04.008
Oliva Marin JE. COVID-19 en niñez y adolescencia. Alerta, Rev científica del Inst Nac Salud [Internet]. 2021;4(1):48-60. Disponible en: https://camjol.info/index.php/alerta/article/view/9780 DOI: 10.5377/alerta.v4i1.9780
Bello-Fernández ZL, Tamayo-Pérez R, Pacheco-Pérez Y, Puente-González ST, Almaguer-Esteve MM. Resistencia antimicrobiana en pacientes ingresados en unidades de cuidados intensivos. Rev Electrónica Dr Zoilo E Mar Vidaurreta [Internet]. 2018;43(6). Disponible en: https://revzoilomarinello.sld.cu/index.php/zmv/article/view/1598
Silva IC, Guzmán M, Montes de Oca M, Bacci S, de Abreu F, Villarroel H, et al. Prescripción de antibióticos en pacientes hospitalizados con COVID-19 en un hospital privado de Caracas: Estudio retrospectivo. Arch Venez Farmacol y Ter [Internet]. 2021;40(4). Disponible en: http://saber.ucv.ve/ojs/index.php/rev_aavft/article/view/23041
Bello-Fernández ZL, Pacheco-Pérez Y, Castillo-Pompa PE, Núñez-Chacón J, Cozme-Rojas Y. Resistencia antimicrobiana en pacientes de la comunidad. Rev Electrónica Dr Zoilo E Mar Vidaurreta [Internet]. 2019;44(4). Disponible en: https://revzoilomarinello.sld.cu/index.php/zmv/article/view/1877
Zaror L, Aravena A, Valenzuela A. Mycosis in times of COVID-19. Rev Bras Análises Clínicas [Internet]. 2021;53(2). Disponible en: https://www.rbac.org.br/artigos/micosis-en-tiempos-de-covid-19/ DOI: 10.21877/2448-3877.202100965
Salehi M, Ahmadikia K, Mahmoudi S, Kalantari S, Jamalimoghadamsiahkali S, Izadi A, et al. Oropharyngeal candidiasis in hospitalised COVID-19 patients from Iran: Species identification and antifungal susceptibility pattern. Mycoses [Internet]. 2020;63(8):771-8. Disponible en: https://doi.org/10.1111/myc.13137 DOI: 10.1111/myc.13137 PMID 32609906 PMCID PMC7361944
Azoulay E, Russell L, Van de Louw A, Metaxa V, Bauer P, Povoa P, et al. Diagnosis of severe respiratory infections in immunocompromised patients. Intensive Care Med [Internet]. 2020;46(2):298-314. Disponible en: https://doi.org/10.1007/s00134-019-05906-5 DOI: 10.1007/s00134-019-05906-5 PMID 32034433 PMCID PMC7080052
Lv Z, Cheng S, Le J, Huang J, Feng L, Zhang B, et al. Clinical characteristics and co-infections of 354 hospitalized patients with COVID-19 in Wuhan, China: a retrospective cohort study. Microbes Infect [Internet]. 2020;22(4):195-9. Disponible en: https://www.sciencedirect.com/science/article/pii/S128645792030085X DOI: 10.1016/j.micinf.2020.05.007 PMID 32425649 PMCID PMC7233257
Rangel K, Chagas TP, De-Simone SG. Acinetobacter baumannii Infections in Times of COVID-19 Pandemic. Pathogens [Internet]. 2021;10(8). Disponible en: https://www.mdpi.com/2076-0817/10/8/1006 DOI: 10.3390/pathogens10081006 PMID 34451470 PMCID PMC8399974
Fernández P, Moreno L, Yagüe G, Andreu E, Jara R, Segovia M. Colonización por microorganismos multirresistentes en pacientes de UCI durante la pandemia de la COVID-19. Med Intensiva [Internet]. 2021;45(5):313-5. Disponible en: https://www.medintensiva.org/es-colonizacion-por-microorganismos-multirresistentes-pacientes-articulo-S0210569121000358 DOI: 10.1016/j.medin.2021.02.015
Laffita-Matos R, Bello-Fernández ZL, Pacheco-Pérez Y, Cutié-Aragón Y, Puente-González ST. Resistencia antimicrobiana de bacterias aisladas en neonatos ingresados en el hospital provincial de Las Tunas en el 2020. Rev Electrónica Dr Zoilo E Mar Vidaurreta [Internet]. 2022;47(1). Disponible en: https://revzoilomarinello.sld.cu/index.php/zmv/article/view/2997
Moncada PA, Candelo KD, Sierra-Ruiz M, Rosso F, Martínez LF, De Paz DA, et al. Infección invasiva por Candida spp. En pacientes inmunocomprometidos: Descripción de curso clínico experiencia diagnóstica, manejo y seguimiento en centro de alta complejidad. Infectio [Internet]. 2020;24(3):143. Disponible en: https://revistainfectio.org/P_OJS/index.php/infectio/article/view/858 DOI: 10.22354/in.v24i3.858
Herreras Gómez LR, López VC. Antifungal resistance profile in the treatment of vaginal candidiasis: A diagnosis of etiologic agents. Rev Habanera Ciencias Médicas [Internet]. 2022;21(2):1-7. Disponible en: https://revhabanera.sld.cu/index.php/rhab/article/view/4241
Zurita Macalupú S. Situación de la resistencia antifúngica de especies del género Cándida en Perú. Rev Peru Med Exp Salud Publica [Internet]. 2018;35(1):126-31. Disponible en: https://doi.org/10.17843/rpmesp.2018.351.3563 DOI: 10.17843/rpmesp.2018.351.3563
Copyright (c) 2024 Maribel Josefina Castellano-González, Mairelin Delia Chirinos Cárdenas, Rosmery Carolay Sanz-Ferrer, Isabelle Virginia Sandoval-Castellano

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Kasmera journal is registered under a Creative Commons an Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), available at: https://creativecommons.org/licenses/by-nc-sa/4.0/deed.en; which guarantees the freedom to share-copy and redistribute the material in any medium or format and adapt-remix, transform and build from the material, provided that the name of the authors, the Department of Infectious and Tropical Diseases, Zulia´s University and Kasmera Journal, you must also provide a link to the original document and indicate if changes have been made.
The Department of Infectious and Tropical Diseases, University of Zulia and Kasmera Journal do not retain the rights to published manuscript and the contents are the sole responsibility of the authors, who retain their moral, intellectual, privacy and publicity rights. The guarantee on the intervention of the manuscript (revision, correction of style, translation, layout) and its subsequent dissemination is granted through a license of use and not through a transfer of rights, which represents the Kasmera Journal and Department Infectious Diseases, University of Zulia are exempt from any liability that may arise from ethical misconduct by the authors.
Kasmera is considered a green SHERPA/RoMEO journal, that is, it allows self-archiving of both the pre-print (draft of a manuscript) and the post-print (the corrected and peer-reviewed version) and even the final version (layout as it will be published in the journal) both in personal repositories and in institutional and databases.