Efectos del extracto de Astragalus microcephalus y la combinación de vitamina E y Selenio sobre el daño tisular inducido por Cadmio en ovarios de rata

• Begum Kurt Sivas Cumhuriyet University, Faculty of Medicine, Department of Obstetrics and Gynecology. Sivas,Türkiye
• Haki Kara Sivas Cumhuriyet University, Faculty of Veterinary, Department of Pharmacology and Toxicology. Sivas, Türkiye
• Mahmut Sahın Sivas Cumhuriyet University, Faculty of Veterinary, Department of Pharmacology and Toxicology. Sivas, Türkiye
• Alper Serhat Kumru Sivas Cumhuriyet University, Faculty of Veterinary, Department of Pharmacology and Toxicology. Sivas, Türkiye
• Mustafa Ozcaraca Sivas Cumhuriyet University, Faculty of Veterinary Medicine, Department of Pathology. Merkez, Sivas, Türkiye

DOI: https://doi.org/10.52973/rcfcv-e34449

Palabras clave: Cadmio, daño ovárico, astrágalo, estrés oxidativo

Resumen

Este estudio se realizó para investigar el efecto protector del extracto de astrágalo (AST) solo y en combinación con vitamina E (vit E) + selenio (Se) contra la toxicidad inducida por cadmio (CdCl₂) en ovarios de rata. Treinta y seis ratas Wistar hembra se dividieron en seis grupos. Se administró AST a una dosis de 5 mg·kg^-1, Cd a una dosis de 2 mg·kg^-1 y Vit E (60 mg·kg^-1) + Se (1 mg·kg^-1) por vía oral durante 15 días. Se analizaron los niveles de MDA, GSH–Px, SOD y CAT en muestras de sangre y tejido para evaluar el estrés oxidativo. Además, en las muestras de suero se midieron los niveles de estrógeno, FSH, LH, inhibina B y hormonas antimullerianas. Los tejidos ováricos se examinaron histopatológica e inmunohistoquímicamente para determinar la inmunorreactividad de 8–OhDG, caspasa 3 y LC3B. En el grupo expuesto a CdCl₂, los niveles de MDA aumentaron significativamente, mientras que los parámetros antioxidantes mostraron disminuciones significativas (P<0,05). Aunque se observaron mejoras significativas en los grupos tratados con AST sola, se observaron mejoras más significativas en los grupos tratados tanto con AST como con Vit E + Se (P<0,05). Se concluyó que los extractos de AST solos y en combinación con Vit E + Se exhibieron efectos protectores contra la toxicidad ovárica causada por la exposición al Cd y pueden ser eficaces contra la toxicidad de los metales.

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