Ruminal degradation and methane production in ruminants fed forage and pumpkin seed oil (Curcubita moschata)

• René Pinto-Ruiz Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas. Villaflores, Chiapas, México
• Roselia Ramírez-Díaz Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas. Villaflores, Chiapas, México
• David Hernández-Sánchez Programa de Ganadería, Colegio de Posgraduados. Montecillo, estado de México, México
• Manuel La O–Arias Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas. Villaflores, Chiapas, México
• José Apolonio Venegas-Venegas Universidad Autónoma de Chiapas, Facultad de Ciencias Agronómicas. Villaflores, Chiapas, México

DOI: https://doi.org/10.52973/rcfcv-e34473

Keywords: Pumpkin, unsaturated fatty acids, fermentation kinetics, fermentation fractions, methane

Abstract

The use of vegetable oils is an option to reduce methane production in ruminal fermentation. In the present work, the effect of pumpkin seed oil (CSA) on CH₄ production, rumen fermentation kinetics and dry matter degradation was evaluated, integrated into ruminant diets using the in vitro gas production technique. The treatments evaluated were six adding different levels of ASC to a base diet that consisted of Cynodon nlemfuensis: T1= 0% ASC+ 100% C. nlemfuensis; T2= 2% ASC + 98% C. nlemfuensis; T3= 3% ASC + 97% C. nlemfuensis; T4= 4% ASC + 96% C. nlemfuensis; T5= 5% ASC + 95% C. nlemfuensis; T6= 7% ASC + 93% C. nlemfuensis, 10 repetitions were used for each treatment analyzed under a completely randomized design and the means were evaluated through the Tukey test (P<0.05) using the statistical package for all of this. SAS. The unsaturated fatty acid content of the BSA was 67.2%, with the most abundant being linoleic and oleic acids (46.16 and 20.10% respectively). Regarding the results in methane (CH₄) production, the ASC did not produce significant changes when it was incorporated into the diets, however, the medium fermentation fractions were improved and the slow fermentation fractions were reduced. On the other hand, when using ASC, raising the proportions to 4% affected by the degradation of dry matter (DGRMS), affecting the degradation of the fiber. According to what was obtained, inconsistency and variability was observed in the results, so the implementation of ASC as a CH₄ mitigation strategy is not suggested due to the negative effects caused by DGRMS, despite meeting the characteristics that other oils have presented and are suggests deepening the chemical aspects of ASC.

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