Assessment of Phenotypic Methods for Metalobetalactamase Detection in Clinical Isolates of Pseudomonas aeruginosa

  • Armindo José Perozo Mena Chair of Professional Practice of Bacteriology. School of Bioanalysis. Faculty of Medicine. University of Zulia. Bacteriological Reference Center. Autonomous Service Maracaibo University Hospital.
  • Maribel Josefina Castellano González Chair of General Bacteriology. School of Bioanalysis. Faculty of Medicine. University of Zulia.
  • Kathyuska Tutaya Chávez Bacteriological Reference Center. Autonomous Service Maracaibo University Hospital.
  • Eliana Ling Toledo Bacteriological Reference Center. Autonomous Service Maracaibo University Hospital.
  • Nailet Arraiz School of Bioanalysis. Faculty of Medicine. University of Zulia. Molecular Biology Laboratory. Dr. Feliz Gómez Metabolic Endocrine Research Center. School of Medicine. University of Zulia.
Keywords: P. aeruginosa, metalo-beta-lactamase, detection, MBL, blaIMP, blaVIM

Abstract

Pseudomonas aeruginosa is considered one of the most important nosocomial pathogens. Its isolation is common in intensive care unit patients who also usually have multi-resistance. This greatly limits therapeutic options, since no effec tive drugs are available for treatment. The study
aims to evaluate four phenotypic methods for detecting metallo-beta-lactamases. The double disc synergy with EDTA method, combined imipenem and meropenem disk with EDTA method, the E-Test-MBL method and the modified Hodge method, along with a reference method (PCR), are used to determine the phenotypic method or a combination of these is used that is more valid and safe for implementation in routine bacteriology laboratories. The sensitivity and specificity of the phenotypic methods used was good (over 90%); the modified Hodge test obtained the best results in detecting MBL-producing P. aeruginosa isolates (lower rate of false positive and negative). On analyzing the different combined methods for determining the association with greater sensitivity and specificity, the double disc method associated with the modified Hodge test met these characteristics. Results suggest that these two methods should be routinely used in a bacteriology laboratory, since they are easy to per form, sensi tive, specific and economical.

Published
2013-10-14
How to Cite
1.
Perozo Mena AJ, Castellano González MJ, Tutaya Chávez K, Ling Toledo E, Arraiz N. Assessment of Phenotypic Methods for Metalobetalactamase Detection in Clinical Isolates of Pseudomonas aeruginosa. Kasmera [Internet]. 2013Oct.14 [cited 2025Jun.27];41(2):115-26. Available from: https://produccioncientificaluz.org/index.php/kasmera/article/view/8319
Issue
Vol. 41 No. 2 (2013)
Section
Original Articles

Copyright (c) 2013 Armindo José Perozo Mena, Maribel Josefina Castellano González, Kathyuska Tutaya Chávez, Eliana Ling Toledo, Nailet Arraiz

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