Comparison Between Inmunochromatographic and Enzyme Linked Immunosorbent Assay (ELISA) in the Dengue Diagnosis

  • Nereida Valero Virology Section, Clinical Research Institute “Dr. Américo Negrette. ” University of Zulia. Venezuela.
  • Milagros Montiel Department of Microbiology, Professional Immunology Practice. School of Bioanalysis. Faculty of Medicine, University of Zulia. Venezuela.
  • Julia Arias Department of Microbiology, Professional Immunology Practice. School of Bioanalysis. Faculty of Medicine, University of Zulia. Venezuela.
  • Belkis Fuentes Departamento de Salud Pública y Social, Práctica Profesional Asistencial/agroindustrial, Escuela de Bioanálisis. Facultad de Medicina, Universidad del Zulia. Maracaibo.
  • Alibeth Mavarez Virology Section, Clinical Research Institute “Dr. Américo Negrette. ” University of Zulia. Venezuela.
  • Lesmy Nava Virology Section, Clinical Research Institute “Dr. Américo Negrette. ” University of Zulia. Venezuela.
  • José Hernández Virology Section, Clinical Research Institute “Dr. Américo Negrette. ” University of Zulia. Venezuela.
Keywords: Antibodies, enzymatic immune-analysis, immune-chromatography

Abstract

The search for techniques for the diagnostic confirmation of Dengue (IgM and IgG) and recent commercialization of such serums requires of the study of their sensibility, specificity, reproductibility, speed and costs. For the purpose of comparing two serological techniques for the diagnosis of this infection, 184 serum samples of patients with confirmed diagnosis of Dengue and 60 with Rubella, were analyzed by immune-chromatographic (IC) and enzyme linked immune-absorbent tests (ELISA) by determination of anti-dengue antibody IgM and IgG. In the IC and ELISA techniques levels of 0,70 and 0,79 in nosologic sensibility were obtained, respectively in the determination of antibody IgM; while the sensibility of ELISA IgG resulted significantly elevated (p<0,0001) with respect to IC (0,92 vs 0,65). Both techniques reached reproduction levels of 98% and a specificity of 100%. These results suggest that ELISA is best for the detection of primary and secondary infections, while IC was the fastest assay and applicable in outbreaks and epidemics; however, it was less sensitive for the diagnosis of secondary infections for dengue virus and consequently less adequate for the screening of dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS) in endemic areas.

Published
2006-06-15
How to Cite
1.
Valero N, Montiel M, Arias J, Fuentes B, Mavarez A, Nava L, Hernández J. Comparison Between Inmunochromatographic and Enzyme Linked Immunosorbent Assay (ELISA) in the Dengue Diagnosis. Kasmera [Internet]. 2006Jun.15 [cited 2024Nov.13];34(1):53-0. Available from: https://produccioncientificaluz.org/index.php/kasmera/article/view/4769
Issue
Vol. 34 No. 1 (2006)
Section
Original Articles

Copyright (c) 2006 Nereida Valero, Milagros Montiel, Julia Arias, Belkis Fuentes, Alibeth Mavarez, Lesmy Nava, José Hernández

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