EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA

  • B Calvo University of Zulia. Maracaibo
  • A Colombo Federal University of Sao Paulo, Paulista School of Medicine. Sao Paulo
  • O Fischman Federal University of Sao Paulo, Paulista School of Medicine. Sao Paulo
  • A Santiago Clinical hospital. Caracas
  • F Nunes Federal University of Sao Paulo, Paulista School of Medicine. Sao Paulo
  • Branchini, M.L Branchini State University of Campinas. Campinas
Keywords: Susceptibility, karyotype, Cryptococcus neoformans, Venezuela

Abstract

Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimicotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classification was based on growth in CGB agar medium, serotyping was performed by slide agglutination with five specific sera. Antifungal susceptibility was performed for amphothericin B, fluconazole, itroconazole and 5-flucytosine, according to NCCLS recommendations and karyotype study was done through contour clamped homogenous field gel electrophoresis (CHEF). Twenty seven (27) isolates of C. neoformans var. neoformans were obtained,all being of serotype A, and three (3) isolates of C. neoformans var. gattii, two (2) belonging to serotype B and one (1) to serotype C. Strains of this series were highly susceptible to the antifungal agents tested. Electrophoretic Karyotype (EK) analysis showed 13 profiles, presenting between 7 and 9 chromosomes and the molecular weight variation was 450/565 and 2200 kb. No significant differences were observed between the minimum inhibitory concentrations (MIC), nor was it possible to associate EK profiles among the isolates of HIV and non HIV patients. Further clinical and laboralorial investigations are mandatory to improve the understanding of C. neoformans in Venezuela.

Published
2000-03-06
How to Cite
1.
Calvo B, Colombo A, Fischman O, Santiago A, Nunes F, Branchini BM. EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA. Kasmera [Internet]. 2000Mar.6 [cited 2024Jul.7];28(3). Available from: https://produccioncientificaluz.org/index.php/kasmera/article/view/4671
Issue
Vol. 28 No. 3 (2000)
Section
Original Articles

Copyright (c) 2000 B Calvo, A Colombo, O Fischman, A Santiago, F Nunes, Branchini, M.L Branchini

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Kasmera journal is registered under a Creative Commons an Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), available at: https://creativecommons.org/licenses/by-nc-sa/4.0/deed.en; which guarantees the freedom to share-copy and redistribute the material in any medium or format and adapt-remix, transform and build from the material, provided that the name of the authors, the Department of Infectious and Tropical Diseases, Zulia´s University and Kasmera Journal, you must also provide a link to the original document and indicate if changes have been made.

The Department of Infectious and Tropical Diseases, University of Zulia and Kasmera Journal do not retain the rights to published manuscript and the contents are the sole responsibility of the authors, who retain their moral, intellectual, privacy and publicity rights. The guarantee on the intervention of the manuscript (revision, correction of style, translation, layout) and its subsequent dissemination is granted through a license of use and not through a transfer of rights, which represents the Kasmera Journal and Department Infectious Diseases, University of Zulia are exempt from any liability that may arise from ethical misconduct by the authors.

Kasmera is considered a green SHERPA/RoMEO journal, that is, it allows self-archiving of both the pre-print (draft of a manuscript) and the post-print (the corrected and peer-reviewed version) and even the final version (layout as it will be published in the journal) both in personal repositories and in institutional and databases.