Resistance mechanisms against beta-lactams antibiotics in Enterobacterales isolated in blood cultures, Maracay, Aragua state, Venezuela
Abstract
The Increased drug resistance among Enterobacterales is a serious problem. The aim was to evaluate the resistance against beta-lactams antibiotics of Enterobacterales isolated in blood cultures. The isolates were identified by phenotypic tests, antimicrobial susceptibility was performed by Kirby-Bauer method and Mechanisms of resistance: extended-spectrum-β-lactamases (ESBL), serinocarbapenemases (KPC), metalocarbapenemases and AmpC enzymes were evaluated by phenotypic methods. Seventy-three (73) strain were confirmed as Enterobacterales being most commonly identified Klebsiella pneumoniae (34.3 %), Enterobacter cloacae complex (19.2 %), Serratia marcescens (20.6 %), Enterobacter gergoviae (11.0 %). A proportion of E. cloacae complex, K. pneumoniae and E. coli isolated, were resistant against beta-lactams, aminoglycosides and quinolones. ESBL, KPC, metalocarbapenemases and AmpC phenotypes were identified in 78 % of Enterobacterales strains. All species presented ESBL and the highest proportion was observed in E. gergoviae (100 %), E. cloacae complex (84,3 %) and S. marcescens (80.0 %). metalocarbapenemases and KPC phenotypes were mainly observed in K. pneumoniae strain with 28 % and 18 %, respectively, among isolate of this specie. AmpC types were detected in Enterobacter and Serratia being a mechanism encoded at the chromosomal level. The presence of different mechanisms of resistance to beta-lactams was detected among Enterobacterales isolated from blood cultures
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