Observational study of the aerobic gut microbiota
Abstract
The microbial ecosystem associated with the intestine is the most diverse and complex in the human being, since it changes during the different stages of life. Losing balance of this intestinal ecosystem is known as dysbiosis. To establish the possible relationship between aerobic dysbiosis and diseases of clinical interest found. Three hundred and seventy-seven (377) cases of dysbiosis with different pathologies such as autism spectrum disorder (ASD), inflammatory bowel disease, allergies, food intolerance, among others; and a control group of 497 apparently healthy people, were studied. For the analysis of the information, multinomial logistic regression and Student's t tests were used, adjusted to a 95% confidence interval. The growth of beneficial microbiota showed variability in the dysbiosis case group compared to the control group (p <0.001). The transient microbiota causing dysbiosis was mainly made up of gram-positive cocci: Streptococcus viridans group, Enterococcus faecium and Enterococcus gallinarum; gram-negative bacilli: Klebsiella pneumoniae, Enterobacter cloacae complex, and Klebsiella oxytoca and Candida albicans, Candida tropicalis and Candida parapsilosis complex species. The most susceptible population with dysbiosis was children aged between 2 and 6 years. The ASD and food intolerance were significantly associated with dysbiosis (p <0.05).References
Moreno X, Vialva A. Generalidad en microbiota intestinal. Acta Científica la Soc Venez Bioanalistas Espec [Internet]. 2020;22(1):27-34. Disponible en: http://saber.ucv.ve/ojs/index.php/rev_ACSVBE/article/view/18807
Alarcón Cavero T, D´Auria G, Delgado Palacio S, Campo Moreno R, Ferrer Martínez M. Microbiota. Procedimientos en Microbiología Clínica [Internet]. Cercenado Mansilla E, Cantón Moreno R, editores. Madrid: Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica; 2016. 1-43 p. Disponible en: https://www.seimc.org/contenidos/documentoscientificos/procedimientosmicrobiologia/seimc-procedimientomicrobiologia59.pdf
Icaza-Chávez ME. Microbiota intestinal en la salud y la enfermedad. Rev Gastroenterol México [Internet]. 2013;78(4):240-8. Disponible en: http://www.sciencedirect.com/science/article/pii/S0375090613001468 DOI: 10.1016/j.rgmx.2013.04.004 PMID 24290319 Google Académico Microsoft Académico
Quera P R, Quigley EMM, Madrid S AM. Sobrecrecimiento bacteriano intestinal. Rev Med Chil [Internet]. 2005;133(11):1361-70. Disponible en: https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98872005001100013&lng=en&nrm=iso&tlng=en DOI: 10.4067/S0034-98872005001100013 PMID 16446861 SciELO Google Académico Microsoft Académico
Chan YK, Estaki M, Gibson DL. Clinical Consequences of Diet-Induced Dysbiosis. Ann Nutr Metab [Internet]. 2013;63(Suppl. 2):28-40. Disponible en: https://www.karger.com/DOI/10.1159/000354902 DOI: 10.1159/000354902 PMID 24217034 Google Académico Microsoft Académico
O’ Mahony SM, Stilling RM, Dinan TG, Cryan JF. The microbiome and childhood diseases: Focus on brain-gut axis. Birth Defects Res Part C Embryo Today Rev [Internet]. 1 de diciembre de 2015;105(4):296-313. Disponible en: https://doi.org/10.1002/bdrc.21118 DOI: 10.1002/bdrc.21118 PMID 26706413 Google Académico Microsoft Académico
Samsam M, Ahangari R, Naser SA. Pathophysiology of autism spectrum disorders: Revisiting gastrointestinal involvement and immune imbalance [Internet]. World J Gastroenterol. 2014;20(29);9942-51. Disponible en: http://www.wjgnet.com/1007-9327/full/v20/i29/9942.htm DOI: 10.3748/wjg.v20.i29.9942 PMID 25110424 PMCID PMC4123375 Google Académico Microsoft Académico
Moreno X, Santamaria G, Sánchez R, De la Torre B, Garcés F, Hernández C, et al. Microbiota gastrointestinal aeróbica en niños con trastornos del espectro autista. Estudio Preliminar. Rev GEN [Internet]. 14 de abril de 2015;69(2):36-44. Disponible en: http://www.genrevista.org/index.php/GEN/article/view/12 Google Académico Microsoft Académico
Mezzelani A, Landini M, Facchiano F, Raggi ME, Villa L, Molteni M, et al. Environment, dysbiosis, immunity and sex-specific susceptibility: A translational hypothesis for regressive autism pathogenesis. Nutr Neurosci [Internet]. 1 de mayo de 2015;18(4):145-61. Disponible en: https://doi.org/10.1179/1476830513Y.0000000108 DOI: 10.1179/1476830513Y.0000000108 PMID 24621061 PMCID PMC4485698 Google Académico Microsoft Académico
Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, et al. Human gut microbiome viewed across age and geography. Nature [Internet]. 2012;486(7402):222-7. Disponible en: https://doi.org/10.1038/nature11053 DOI: 10.1038/nature11053 PMID 22699611 PMCID PMC3376388 Google Académico Microsoft Académico
Cani PD, Knauf C. How gut microbes talk to organs: The role of endocrine and nervous routes. Mol Metab [Internet]. 2016;5(9):743-52. Disponible en: http://www.sciencedirect.com/science/article/pii/S2212877816300485 DOI: 10.1016/j.molmet.2016.05.011 PMID 27617197 PMCID PMC5004142 Google Académico Microsoft Académico
Sherwin E, Sandhu K V, Dinan TG, Cryan JF. May the Force Be With You: The Light and Dark Sides of the Microbiota–Gut–Brain Axis in Neuropsychiatry. CNS Drugs [Internet]. 2016;30(11):1019-41. Disponible en: https://doi.org/10.1007/s40263-016-0370-3 DOI: 10.1007/s40263-016-0370-3 PMID 27417321 PMCID PMC5078156 Google Académico Microsoft Académico
Delgado Palacio S. Microbiota Intestinal Humana: Análisis y evolución de poblaciones representativas e identificación de bacterias probióticas [Tesis Doctoral]. Universidad de Oviedo. Departamento de Biología Funcional y Molecular. Instituto de Productos Lácteos de Asturias; 2005. Disponible en: http://hdl.handle.net/10261/5220 Google Académico Microsoft Académico
Kruis W, Frič P, Pokrotnieks J, Lukáš M, Fixa B, Kaščák M, et al. Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine. Gut [Internet]. 1 de noviembre de 2004;53(11):1617-23. Disponible en: http://gut.bmj.com/content/53/11/1617.abstract DOI: 10.1136/gut.2003.037747 PMID 15479682 PMCID PMC1774300 Google Académico Microsoft Académico
Henker J, Laass MW, Blokhin BM, Maydannik VG, Bolbot YK, Elze M, et al. Probiotic Escherichia coli Nissle 1917 versus placebo for treating diarrhea of greater than 4 days duration in infants and toddlers. Pediatr Infect Dis J [Internet]. 2008;27(6):494-9. Disponible en: https://journals.lww.com/pidj/Fulltext/2008/06000/Probiotic_Escherichia_coli_Nissle_1917_Versus.3.aspx DOI: 10.1097/INF.0b013e318169034c PMID 18469732 Google Académico Microsoft Académico
Matthes H, Krummenerl T, Giensch M, Wolff C, Schulze J. Clinical trial: probiotic treatment of acute distal ulcerative colitis with rectally administered Escherichia coli Nissle 1917 (EcN). BMC Complement Altern Med [Internet]. 2010;10(1):13. Disponible en: https://doi.org/10.1186/1472-6882-10-13 DOI: 10.1186/1472-6882-10-13 PMID 20398311 PMCID PMC2861635 Google Académico Microsoft Académico
Guarner F. Microbiota intestinal y enfermedades inflamatorias del intestino. Gastroenterol Hepatol [Internet]. 2011;34(3):147-54. Disponible en: http://www.sciencedirect.com/science/article/pii/S0210570511000379 DOI: 10.1016/j.gastrohep.2010.11.009 PMID 21377761 Google Académico Microsoft Académico
Neufeld KM, Kang N, Bienenstock J, Foster JA. Reduced anxiety-like behavior and central neurochemical change in germ-free mice. Neurogastroenterol Motil [Internet]. 1 de marzo de 2011;23(3):255-e119. Disponible en: https://doi.org/10.1111/j.1365-2982.2010.01620.x DOI: 10.1111/j.1365-2982.2010.01620.x PMID 21054680 Google Académico Microsoft Académico
Bravo JA, Forsythe P, Chew M V, Escaravage E, Savignac HM, Dinan TG, et al. Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve. Proc Natl Acad Sci [Internet]. 20 de septiembre de 2011;108(38):16050-16055. Disponible en: http://www.pnas.org/content/108/38/16050.abstract DOI: 10.1073/pnas.1102999108 PMID 21876150 PMCID PMC3179073 Google Académico Microsoft Académico
Moreno X, Santamaria G, Sánchez R, et al. Disbiosis aeróbica y síntomas gastrointestinales y conductuales en niños con trastorno del espectro autista. Acta Científica la Soc Venez Bioanalistas Espec. 2018; 21(2): 36-44.
Moreno X, Olivares A, Sánchez R, Santamaría G, Garcés F, Márquez A, et al. Disbiosis en niños con Trastorno Autista antes y después de un tratamiento biológico y nutricional. Arch Ven Ped Puercult. [Internet]. 2019; 82(1):15-20. Disponible en: http://www.svpediatria.org/repositorio/publicaciones/2019/AVPP%2082-1.pdf
Adams JB, Johansen LJ, Powell LD, Quig D, Rubin RA. Gastrointestinal flora and gastrointestinal status in children with autism – comparisons to typical children and correlation with autism severity. BMC Gastroenterol [Internet]. 2011;11(1):22. Disponible en: https://doi.org/10.1186/1471-230X-11-22 DOI: 10.1186/1471-230X-11-22 PMID 21410934 PMCID PMC3072352 Google Académico Microsoft Académico
Weidinger S, Beck LA, Bieber T, Kabashima K, Irvine AD. Atopic dermatitis. Nat Rev Dis Prim [Internet]. 2018;4(1):1. Disponible en: https://doi.org/10.1038/s41572-018-0001-z DOI: 10.1038/s41572-018-0001-z PMID 29930242
Ash C. Bacterial involvement in autoimmunity. Science [Internet]. 9 de marzo de 2018;359(6380):1114-1116. Disponible en: http://science.sciencemag.org/content/359/6380/1114.13.abstract DOI: 10.1126/science.359.6380.1114-m Google Académico Microsoft Académico
Garn H, Neves JF, Blumberg RS, Renz H. Effect of barrier microbes on organ-based inflammation. J Allergy Clin Immunol [Internet]. 1 de junio de 2013;131(6):1465-78. Disponible en: https://doi.org/10.1016/j.jaci.2013.04.031 DOI: 10.1016/j.jaci.2013.04.031 PMID 23726530 PMCID PMC4592166 Google Académico Microsoft Académico
Lepage P, Häsler R, Spehlmann ME, Rehman A, Zvirbliene A, Begun A, et al. Twin study indicates loss of interaction between microbiota and mucosa of patients with ulcerative colitis. Gastroenterology [Internet]. 1 de julio de 2011;141(1):227-36. Disponible en: https://doi.org/10.1053/j.gastro.2011.04.011 DOI: 10.1053/j.gastro.2011.04.011 PMID 21621540 Google Académico Microsoft Académico
Li X-X, Wong GL-H, To K-F, Wong VW-S, Lai LH, Chow DK-L, et al. Bacterial microbiota profiling in gastritis without Helicobacter pylori infection or non-steroidal anti-inflammatory drug use. PLoS One [Internet]. 24 de noviembre de 2009;4(11):e7985. Disponible en: https://doi.org/10.1371/journal.pone.0007985 DOI: 10.1371/journal.pone.0007985 PMID 19956741 PMCID PMC2776972 Google Académico Microsoft Académico
Valverde-Molina J, Valverde-Fuentes J. La disbiosis microbiana como origen precoz del asma. Rev Asma [Internet]. 2018;3(2):36-45. Disponible en: https://www.separcontenidos.es/revista3/index.php/revista/article/view/138/185 Google Académico Microsoft Académico
Duboc H, Rainteau D, Rajca S, Humbert L, Farabos D, Maubert M, et al. Increase in fecal primary bile acids and dysbiosis in patients with diarrhea-predominant irritable bowel syndrome. Neurogastroenterol Motil [Internet]. 1 de junio de 2012;24(6):513-e247. Disponible en: https://doi.org/10.1111/j.1365-2982.2012.01893.x DOI: 10.1111/j.1365-2982.2012.01893.x PMID 22356587 Google Académico Microsoft Académico
Marotz CA, Zarrinpar A. Treating obesity and metabolic syndrome with fecal microbiota transplantation. Yale J Biol Med [Internet]. septiembre de 2016;89(3):383-8. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045147/ PMID 27698622 PMCID PMC5045147 Microsoft Académico
Patterson E, Ryan PM, Cryan JF, Dinan TG, Ross RP, Fitzgerald GF, et al. Gut microbiota, obesity and diabetes. Postgrad Med J [Internet]. 1 de mayo de 2016;92(1087):286-300. Disponible en: http://pmj.bmj.com/content/92/1087/286.abstract DOI: 10.1136/postgradmedj-2015-133285 PMID 26912499 Google Académico Microsoft Académico
Auchtung TA, Fofanova TY, Stewart CJ, Nash AK, Wong MC, Gesell JR, et al. Investigating colonization of the healthy adult gastrointestinal tract by fungi. mSphere [Internet]. 25 de abril de 2018;3(2):e00092-18. Disponible en: http://msphere.asm.org/content/3/2/e00092-18.abstract DOI: 10.1128/mSphere.00092-18 PMID 29600282 PMCID PMC5874442 Google Académico Microsoft Académico
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