Staphylococcus spp. in periodontal pockets from a group of patients attending a dental center in the Municipality of San Diego, Carabobo State

  • Davide Mobili-Rocaro University of Carabobo, School of Bioanalysis
  • Daniela Falcón University of Carabobo, School of Bioanalysis
  • Mariangel Rodríguez University of Carabobo, School of Bioanalysis
Keywords: Staphylococcus spp, Periodontal disease, Periodontal pockets

Abstract

Some unusual microorganisms have been isolated from samples  of  patients  with  oral  cavity  periodontal  disease.  The  objective  of  this  research  was  to  determine  the  frequency of  Staphylococcus  spp.  in  periodontal  pockets  based  on  the  epidemiological  characteristics   of patients attending a dental center  in  the  municipality  of  San  Diego,  Carabobo  state.  Samples from periodontal pockets  of  50  patients  attending  the  dental  center  were studied.  The isolated strains were identified by biochemical tests and finally the profile of antimicrobial susceptibility was determined. It was found that the frequency of patients with Staphylococcus spp. was 32%, 16 patients. Staphylococcus Coagulase Negative (SCN) was isolated in 10 patients, followed by S. aureus in 6. Regarding to antimicrobial susceptibility profile, it was determined that of the 6 isolated strains of Staphylococcus aureus, 2 proved to be Methicillin-resistant. The presence of Staphylococcus spp. in periodontal pockets is low frequency; however in this study, the presence of said microorganism was determined in 32% of the patients studied, which means that these patients are more colonized than what is believed.

References

Escudero N, Perea M, Martínez B. Revisión de la periodontitis crónica: evolución y su aplicación clínica. Avances en Periodoncia. 2008; 20: 27-37.

Newman Carranza F, Newman M, Takei H, Klokkevold P. Periodontologia clinica. 9na ed. México: McGraw-Hill Interamericana; 2010. p.25-75.

Carranza A, Rivera P, Gutiérrez R, Hernández F. Endocarditis aguda estafilococcica fatal con periodontitis severa. Re Costarric Cienc Méd. 2000; 21:3-4.

Botero A, Alvear F, Vélez M, Botero L, Velásquez H. Evaluación de los enfoques terapéuticos para las varias formas de enfermedad periodontal. Parte III: Prevalencia de bacilos entéricos y levaduras. Revista Facultad de Odontología Universidad de Antioquia 2008; 20(1): 72-86.

Capozzi E, Mobili D, Martinez I. Portadores nasales de S. aureus en el personal de enfermería de un centro de salud del Estado Carabobo, Venezuela. Kasmera. 2015; 4(2): 2-3

Liebana J. Microbiologia oral. 2da ed. Madrid: Interamericana McGraw Hill; 2002. p. 317-324.

Loberto J, Paiva C, Ferreira S, Cortelli J, Cardoso A. Staphylococcus spp. in the oral cavity and periodontal pockets of chronic periodontitis pacients. Braz J Microbiol. 2004; 35:1-2.

Cuesta A, Jewtuchowicz V, Brusca M, Nastri M, Rosa A. Prevalence of Staphylococcus spp. and Candida spp. in the oral cavity and periodontal pockets of periodontal disease patients. Acta Odontológica Latinoamericana. 2010; 23(1): 20-26.

Mena A. 2011. Estudio microbiológico del surco gingival en pacientes embarazadas que presentan gingivitis asociada a la placa bacteriana y que acuden al Hospital Provincial General Isidro Ayora en el periodo Febrero- Agosto del 2011. Loja: Universidad Nacional de Loja, Área de la Salud Humana [Trabajo de Grado], pp. 108.

Dzul M. 2013. Aplicación básica de los métodos científicos. México. Disponible en línea en: https://www.uaeh.edu.mx/docencia/VI_Presentaciones/licenciatura_en_mercadotecnia/fundamentos_de_metodologia_investigacion/PRES40.pdf . [04-7-2015].

Arias F. El Proyecto de Investigación. 5ta ed. Caracas: Episteme; 2012. P.21-33.

Manzini J. Declaracion de Helsinki: Principios Éticos para la Investigación Médica sobre Sujetos Humanos. Acta bioeth. [Internet]. [Actualizado 2002 dic; acceso 2017 Abr 5]; 6( 2 ):321-334.Disponible en: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S1726-569X2000000200010&lng=es. http://dx.doi.org/10.4067/S1726-569X2000000200010

Gualano M, Ramírez D, Urdaneta L. 2006. Manual de Procedimientos Básicos de Toma de Muestras Microbiológicas en Cavidad Bucal. Mérida: Universidad de los Andes, Facultad de Odontología [Trabajo de Grado], pp. 72.

Koneman E, Elmer W, Allen S. Diagnostico microbiológico/Microbiological Diagnosis. 6º ed. Buenos Aires: Médica Panamericana. 2008. p. 66-600

Clinical Laboratory Standardization Institute. Antimicrobial susceptibility testing. Twentieth Informational Supplement. Document M100/S24: 34(1).2016

Jayatilake J, Bogoda B, Kularatne J, Senanayake M, Tilakaratne A. Occurrence Of Staphylococcus spp. and Candida spp. In The Periodontal Pockets and Oral Cavity Of Patients With Chronic Periodontitis. Proceedings of the Peradeniya Univ. International Research. 2014;18: 4-5.

Villa A, Amezquita A, Tascón A, Jaramillo A. Presencia de Levaduras en Pacientes con Periodontitis. Revista Colombiana de Investigación en Odontología. 2015; 6: 1-7.

McCormack M, Smith A, Akram A, Jackson M, Robertson D, Edwards G. Staphylococcus aureus and the oral cavity: An overlooked source of carriage and infection? Am J Infect Cont. 2015; 43: 35-37.

Moreno S, Parra B, Botero J, Moreno F, Vásquez D, Fernández H, et al. Microbiota periodontal y microorganismos aislados de válvulas cardiacas en pacientes sometidos a cirugía de remplazo valvular en una clínica de la ciudad Cali. Biomédica. 2017; 37(4).

Published
2017-08-07
How to Cite
1.
Mobili-Rocaro D, Falcón D, Rodríguez M. Staphylococcus spp. in periodontal pockets from a group of patients attending a dental center in the Municipality of San Diego, Carabobo State. Kasmera [Internet]. 2017Aug.7 [cited 2024Jul.30];45(1):16-3. Available from: https://produccioncientificaluz.org/index.php/kasmera/article/view/22832
Issue
Vol. 45 No. 1 (2017)
Section
Original Articles

Copyright (c) 2017 Davide Mobili-Rocaro, Daniela Falcón, Mariangel Rodríguez

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Kasmera journal is registered under a Creative Commons an Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), available at: https://creativecommons.org/licenses/by-nc-sa/4.0/deed.en; which guarantees the freedom to share-copy and redistribute the material in any medium or format and adapt-remix, transform and build from the material, provided that the name of the authors, the Department of Infectious and Tropical Diseases, Zulia´s University and Kasmera Journal, you must also provide a link to the original document and indicate if changes have been made.

The Department of Infectious and Tropical Diseases, University of Zulia and Kasmera Journal do not retain the rights to published manuscript and the contents are the sole responsibility of the authors, who retain their moral, intellectual, privacy and publicity rights. The guarantee on the intervention of the manuscript (revision, correction of style, translation, layout) and its subsequent dissemination is granted through a license of use and not through a transfer of rights, which represents the Kasmera Journal and Department Infectious Diseases, University of Zulia are exempt from any liability that may arise from ethical misconduct by the authors.

Kasmera is considered a green SHERPA/RoMEO journal, that is, it allows self-archiving of both the pre-print (draft of a manuscript) and the post-print (the corrected and peer-reviewed version) and even the final version (layout as it will be published in the journal) both in personal repositories and in institutional and databases.