Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

  • Yeiny Ávila R. General Bacteriology School of Bioanalysis. University of Zulia-Venezuela
  • Messaria Ginestre P. General Bacteriology School of Bioanalysis. University of Zulia-Venezuela
  • Kutchynskaya Valero L. Clinical Bacteriology School of Bioanalysis. University of Zulia-Venezuela
  • Maribel Castellano G. General Bacteriology School of Bioanalysis. University of Zulia-Venezuela
  • Sonia Romero A. General Bacteriology School of Bioanalysis. University of Zulia-Venezuela
  • Alfredo López Cardiovascular Surgery Maracaibo University Hospital. Venezuela.
  • Gresleida Rincón V. Clinical Bacteriology School of Bioanalysis. University of Zulia-Venezuela
  • Lisette Sandrea T. Bacteriology Professional Practice. School of Bioanalysis. University of Zulia-Venezuela
Keywords: Methicillin-resistant S. aureus, enterotoxins, biofilm

Abstract

S. aureus has become a public health problem, due to the difficulty of treating infections caused by methicillin-resistant S. aureus (MRSA). The purpose of this research was to determine the production of enterotoxins A, B, C and D and the production of biofilm in clinical isolates of MRSA. Fifty MRSA strains isolated from different types of clinical samples were studied. Detection of enterotoxins was carried out using the technique of reversed phase agglutination, while biofilm production was studied through two tests: Congo red agar and the microplate cell culture method. Enterotoxin production was observed in 9 strains (18%); enterotoxin D (64%) was the most prevalent, followed by B (27%) and A (9%). A significant association was shown between enterotoxin production capacity and the type of sample that came from the strain. Biofilm production was found in 30% and 98% of the strains using the Congo red Agar and microplate cell culture methods, respectively. A correlation of both trials was observed in only 15 strains (30%). It was shown that the microplate cell culture method is more effective for detecting biofilm production in S. aureus strains.

Published
2014-08-07
How to Cite
1.
Ávila R. Y, Ginestre P. M, Valero L. K, Castellano G. M, Romero A. S, López A, Rincón V. G, Sandrea T. L. Enterotoxin and Biofilm Production in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus. Kasmera [Internet]. 2014Aug.7 [cited 2025Jun.14];42(2):105-1. Available from: https://produccioncientificaluz.org/index.php/kasmera/article/view/19498
Issue
Vol. 42 No. 2 (2014)
Section
Original Articles

Copyright (c) 2014 Yeiny Ávila R., Messaria Ginestre P., Kutchynskaya Valero L., Maribel Castellano G., Sonia Romero A., Alfredo López, Gresleida Rincón V., Lisette Sandrea T.

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