Efectos del período de lactancia sobre algunos parámetros sanguíneos y actividades de la enzima ANAE/AcP-asa en asnos domésticos
Resumen
El objetivo fue investigar la influencia del período de lactancia en algunos parámetros hematológicos (glóbulos rojos, leucocitos y sus subtipos, hematocrito, hemoglobina, hemoglobina media, hemoglobina media, volumen volumétrico medio y plaquetas) y bioquímicos (alanina aminotransferasa, nitrógeno ureico en sangre y creatinina sérica), así como en el porcentaje de linfocitos positivos para en asnos hembras domésticas. Para ello, se seleccionaron 20 burras y se dividieron en dos grupos iguales: control (no lactantes, n = 10) y lactantes (n = 10). Se encontraban en la mitad de su período de lactancia (de tres a seis meses de edad), con edades comprendidas entre los cinco y los catorce años. Tras el examen clínico de rutina, se tomaron muestras de sangre de las animales para medir la actividad de la enzima, así como diversos parámetros sanguíneos. Como resultado, los valores de glóbulo rojo, hematocrito y hemoglobina fueron mayores en las asnos hembras lactantes que en las no lactantes (P < 0,05). Se observó que los valores séricos de fueron estadísticamente superiores en animales lactantes (P < 0,05). Si bien la actividad enzimática fue alta en asnos hembras lactantes, los porcentajes de linfocitos positivos para fueron similares entre los grupos (P > 0,05). En conclusión, se observó que la fase media de la lactancia en asnos hembras influyó en varios parámetros sanguíneos, pero no en la actividad enzimática.
Descargas
Citas
Burden F, Thiemann A. Donkeys are different. J. Equine. Vet. Sci. [Internet]. 2015; 35(5):376–382. doi: https://doi.org/f7cs76 DOI: https://doi.org/10.1016/j.jevs.2015.03.005
Fantuz F, Ferraro S, Todini L, Piloni R, Mariani P, Salimei E. Donkey milk concentration of calcium, phosphorus, potassium, sodium and magnesium. Int. Dairy J. [Internet]. 2012; 24(2):143–145. doi: https://doi.org/bd3fth DOI: https://doi.org/10.1016/j.idairyj.2011.10.013
Tafaro A, Magrone T, Jirillo F, Martemucci G, D’Alessandro AG, Amati L, Jirillo E. Immunological properties of donkey’s milk: its potential use in the prevention of atherosclerosis. Curr. Pharm. Des. [Internet]. 2007; 13(36):3711–3717. doi: https://doi.org/b3v9nz DOI: https://doi.org/10.2174/138161207783018590
Kısadere I, Donmez N, Omurzakova N. Serum biochemical reference values of Kyrgyz donkeys (Equus asinus). Comp. Clin. Path. [Internet]. 2019; 28:817–823. doi: https://doi.org/qncj DOI: https://doi.org/10.1007/s00580-018-2864-5
Laus F, Spaterna A, Faillace V, Veronesi F, Ravagnan S, Beribé F, Cerquetella M, Meligrana M, Tesei B. Clinical investigation on Theileria equi and Babesia caballi infections in Italian donkeys. BMC Vet. Res. [Internet]. 2015; 11:100. doi: https://doi.org/f684x4 DOI: https://doi.org/10.1186/s12917-015-0411-z
Gul ST, Ahmad M, Khan A, Hussain I. Haemato- biochemical observations in apparently healthy equine species. Pakistan. Vet. J. [Internet]. 2007 [cited 22 Jun 2025]; 27(4):155-158. Available in: https://goo.su/BeMzpf
Salari F, Ciampolini R, Mariti C, Millanta F, Altomonte I, Licitra R, Auzino B, Ascenzi CD, Bibbiani C, Giuliotti L, Papini RA, Martini M. A multi-approach study of the performance of dairy donkey during lactation: Preliminary results. Ital. J. Anim. Sci. [Internet]. 2019; 18(1):1135–1141. doi: https://doi.org/qncm DOI: https://doi.org/10.1080/1828051X.2019.1623094
Dezzutto D, Barbero R, Valle E, Giribaldi M, Raspa F, Biasato I, Cavallarin L, Bergagna S, McLean A, Gennero MS. Observations of the hematological, hematochemical, and electrophoretic parameters in lactating donkeys (Equus asinus). J. Equine Vet. Sci. [Internet]. 2018; 65:1–5. doi: https://doi.org/qncn DOI: https://doi.org/10.1016/j.jevs.2018.02.013
Bonelli F, Rota A, Corazza M, Serio D, Sgorbini M. Hematological and biochemical findings in pregnant, postfoaling, and lactating jennies. Theriogenology. [Internet]. 2016; 85(7):1233–1238. doi: https://doi.org/f8f98w DOI: https://doi.org/10.1016/j.theriogenology.2015.12.004
Reiter AS, Reed SA. Lactation in horses. Anim. Front. [Internet]. 2023; 13(3):96-100. doi: https://doi.org/qncp DOI: https://doi.org/10.1093/af/vfad003
Kısadere I, Kadyralieva N, Sur E, Dönmez N, Oruç E, Cihan H. Some physiological, hematological values and ANAE-positive lymphocyte rations of domestic donkeys (Equus asinus) in Kyrgyzstan. Kafkas. Univ. Vet. Fak. Derg. [Internet]. 2016; 23(1):165–168. doi: https://doi.org/qncq
Aydin MF, Celik I, Sur E, Oznurlu Y, Telatar T. Enzyme histochemistry of the peripheral blood lymphocytes in Arabian horses. J. Anim. Vet. Adv. [Internet]. 2010 [cited 22 Jun 2025]; 9(5):920–924. Available in: https://goo.su/Xapa DOI: https://doi.org/10.3923/javaa.2010.920.924
Bazzano M, Bonfili L, Eleuteri AM, Serri E, Scollo C, Yaosen Y, Tesei B, Laus F. Assessment of serum amyloid A concentrations and biochemical profiles in lactating jennies and newborn Ragusano donkey foals around parturition and one month after foaling in Sicily. Reprod. Domest. Anim. [Internet]. 2022; 57(3):262–268. doi: https://doi.org/qncr DOI: https://doi.org/10.1111/rda.14048
Kisadere I, Bayraktar M, Salykov R. Some hematological and biochemical reference values of the thoroughbred Appaloosa horse breeds reared in Kyrgyzstan. Comp. Clin. Path. [Internet]. 2019; 28(6):1651–1660. doi: https://doi.org/qncs DOI: https://doi.org/10.1007/s00580-019-02991-2
Aydin MF, Kisadere I. Some hematological parameters and enzyme histochemistry of peripheral bloodleukocytes in Kivircik sheep. Turk. J. Vet. Anim. Sci. [Internet]. 2022; 46(2):275–284. doi: https://doi.org/qnct DOI: https://doi.org/10.55730/1300-0128.4175
Donmez HH, Sur E. Hematology and enzyme histochemistry of the peripheral blood leucocytes in rock partridges (Alectoris graeca). Poult,.,. m. Sci. [Internet]. 2008; 87(1):56–60. doi: https://doi.org/cf693q DOI: https://doi.org/10.3382/ps.2007-00328
Kajikawa N, Kaibuchi K, Matsubara T, Kikkawa U, Takai Y, Nishizuka YA, Itoh K, Tomioka C. A possible role of protein kinase C in signal-induced lysosomal enzyme release. Biochem. Biophys. Res. Commun. [Internet]. 1983; 116(2):743–750. doi: https://doi.org/c2qktq DOI: https://doi.org/10.1016/0006-291X(83)90587-9
Aydin MF, Celik I, Sur E. Investigation of α-naphthyl acetate esterase and acid phosphatase in the peripheral blood leukocytes of greyhounds. Biotech. Histochem. [Internet]. 2012; 87(4):265–272. doi: https://doi.org/fxxj3p DOI: https://doi.org/10.3109/10520295.2011.639717
Catowsky D. Leucocyte cytochemical and immunological techniques. In: Dacie JV, Lewis SM, editors. Practical Hematology. 7th edition. London, Ingland: Churchill Livingstone; 1981. p. 143 – 174.
Colakoglu F, Dönmez HH, Kılıç F. Determination of ANAE and AcP - ase positivity in the peripheral blood of pregnant women with hypothyroidism. Dicle Tıp. Derg. [Internet]. 2019; 46(2):307–313. doi: https://doi.org/qnc2 DOI: https://doi.org/10.5798/dicletip.574829
Bayraktaroglu AG, Simsek O, Kurum A, Arıkan S, Ergun E. Determination of alpha - naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats. Turk. J. Vet. Anim. Sci. [Internet]. 2015; 39(1):57–61. doi: https://doi.org/qnc3 DOI: https://doi.org/10.3906/vet-1407-44
Mariella J, Pirrone A, Gentilini F, Castagnetti C. Hematologic and biochemical profiles in Standardbred mares during peripartum. Theriogenology. [Internet]. 2014; 81(4):526–534. doi: https://doi.org/f5vbtd DOI: https://doi.org/10.1016/j.theriogenology.2013.11.001
Harvey JW, Asquith RL, Patel MG, Kivipelto J, Chen CL, Ott EA. Haematological findings in pregnant, postparturient and nursing mares. Comp. Haematol. Int. [Internet]. 1994 [cited 22 Jun 2025]; 4:25–29. Available in: https://goo.su/VD7ESMh DOI: https://doi.org/10.1007/BF00368262
Deichsel K, Aurich J. Lactation and lactational effects on metabolism and reproduction in the horse mare. Lives. Prod. Sci. [Internet]. 2005; 98(1-2):25–30. doi: https://doi.org/ckn7pp DOI: https://doi.org/10.1016/j.livprodsci.2005.10.003
Hoffman RM, Kronfeld DS, Cooper WL, Harris PA. Glucose clearance in grazing mares is affected by diet, pregnancy, and lactation. J. Anim. Sci. [Internet]. 2003; 81(7):1764– 1771. doi: https://doi.org/qnc4 DOI: https://doi.org/10.2527/2003.8171764x
Dardenne M, de Moraes M, Kelly PA, Gagnerault MC. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endocrinol. [Internet]. 1994; 134(5):2108–2114. doi: https://doi.org/qnc5 DOI: https://doi.org/10.1210/endo.134.5.8156910
Wallaschofski H, Kobsar A, Koksch M, Siegemund A, Hentschel B, Tuschy Lohmann UT, Sokolova O, Eigenthaler M. Prolactin receptor signaling during platelet activation. Horm. Metab. Res. [Internet]. 2003; 35(4):228–235. doi: https://doi.org/b6g3c6
Ousey JC. Endocrinology of pregnancy. In: McKinnon AO, Squires EL, Vaala WE, Varner DD, editors. Equine reproduction. 2nd edition. West Sussex, UK: Wiley- Blackwell; 2011. p. 2418-2427.
Krumrych W, Wisniewski E, Gruendboeck M. Cytochemical demonstration of alpha-naphthyl acetate esterase and acid phosphatase in blood lymphocytes of horses. Bull. Vet. Inst. Pulawy. [Internet]. 1995 [cited 18 Jul 2025]; 39(1):35–38. Available in: https://goo.su/bBHnatB
Ozaydın T, Çelik I, Sur E, Oznurlu Y, Uluısık D. Cytochemistry of peripheral blood leukocytes in thoroughbred foals. Biotech. Histochem. [Internet]. 2013; 88(6):295–301. doi: https://doi.org/qnc6 DOI: https://doi.org/10.3109/10520295.2013.782067
Oruç E, Kısadere I, Kadıralıeva N, Sur E. Comparison of Some Hematological and Biochemical Analysis, ANAE Profile and Nasal Exfoliation Before and After the Race in jumping Horses in Bishkek Region in Kyrgyzstan. Manas. J. Agric. Vet. Life Sci. [Internet]. 2017 [cited 18 July 2025]; 7(1):12–20. Available in: https://goo.su/cfiEp1Q
















