Determinación de los niveles de 17-β estradiol y progesterona en leche de vaca y leche de continuación para bebés mediante ELISA

  • Pınar Şeker Elazig Provincial Directorate of Agriculture and Forestry, Food and Feed Branch, Elazığ, Türkiye
  • Ali Rişvanlı Kyrgyz-Turkish Manas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology. Bishkek, Kyrgyzstan - Firat University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology. Elazığ, Türkiye https://orcid.org/
  • İbrahim Şeker Fırat University, Faculty of Veterinary Medicine, Department of Zootechny, Elazığ, Türkiye
  • Mehmet Çalıcıoğlu Firat University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology. Elazig, Türkiye
Palabras clave: Progesterona, estradiol 17-β, leche, leche de continuación para bebés, ELISA

Resumen

El estudio actual se llevó a cabo para determinar los niveles de 17-β estradiol (E2) y progesterona (P4) en diferentes productos lácteos y en algunas muestras de leche de seguimiento para bebés recolectadas en mercados minoristas de Turquía. Para ello, se analizaron un total de 50 muestras de 8 grupos de productos diferentes con diferentes niveles de grasa, tecnologías de producción y series de producción para determinar los niveles naturales de P4 y E2 utilizando un kit ELISA comercial. El nivel más alto de E2 se determinó en leche entera UHT (3%) (39,20 ± 6,73 pmol·L-1), mientras que el nivel más bajo se encontró en muestras de suero de leche (26,50 ± 1,85 pmol·L-1). Para los niveles de P4, la concentración más alta se encontró en leche de continuación para bebés (<1 año) (45,83 ± 2,95 nmol·L-1) y los valores más bajos se encontraron en muestras de leche pasteurizada (23,00 ± 6,66 nmol·L-1). Además, en este estudio, no se encontraron estadísticamente diferencias entre los grupos de productos para E2 y P4 (P>0.05). En conclusión, los niveles naturales de P4 y E2 se detectaron en todas las leches y leches de continuación para bebés en varios niveles y, en general, sus niveles se asociaron con el nivel de grasa del producto.

Descargas

La descarga de datos todavía no está disponible.

Citas

ABEYAWARDENE, S.A.; HATHORN, D.J.; GLENCROSS, R.G. Concentrations of oestradiol-17 beta and progesterone in bovine plasma and defatted milk during the post-partum anovulatory period, during oestrous cycles and following ovariectomy. Br. Vet. J. 140(5): 458–467. 1984.

ADEBAMOWO, C.A.; SPIEGELMAN, D.; BERKEY, C.S.; DANBY, F.W.; ROCKETT, H.H.; COLDITZ, G.A.; WILLETT, W.C.; HOLMES, M.D. Milk consumption and acne in teenaged boys. J. Amer. Academy Dermatol. 58(5): 787–793. 2008.

AKGUL, A. Statistik Bilgisayar Programı kullanılarak. Tıbbi araştırmalarda istatistiksel analiz teknikleri. 3rd. Ed. Emek Ofset Ltd. Sti, Ankara, Türkiye. Pp 560. 2005.

AKSGLAEDE, L.; JUUL, A.; LEFFERS, H.; SKAKKEBÆK, N.E.; ANDERSSON, A.M. The sensitivity of the child to sex steroids: possible impact of exogenous estrogens. Human Reprod. Update. 12(4): 341–349. 2006.

ALVES, C.; FLORES, L.C.; CERQUEIRA, T.; TORALLES, M. Environmental exposure to endocrine disruptors with estrogenic activity and the association with pubertal disorders in children. Cad. Saúde Públ. 23(5): 1005–1014. 2007.

ANDERSSON, A.M.; SKAKKEBAEK, N.E. Exposure to exogenous estrogens in food: possible impact on human development and health. Eur. J. Endocrinol. 140(6): 477–485. 1999.

AZZOUZ, A.; JURADO-SANCHEZ, B.; SOUHAIL, B.; BALLESTEROS, E. Simultaneous determination of 20 pharmacologically active substances in cow’s milk, goat’s milk, and human breast milk by gas chromatography-mass spectrometry. J. Agric. Food Chem. 59(9): 5125–5132. 2011.

BARREIRO, R.; REGAL, P.; DÍAZ-BAO, M.; FENTE, C.A.; CEPEDA, A. Analysis of naturally occurring steroid hormones in infant formulas by HPLC–MS/MS and contribution to dietary intake. Foods. 4(4): 605–621. 2015.

CHEN, C.; MI, X.; YUAN, Y.; CHEN, G.; REN, L.; WANG, K.; ZHU, D.; QIAN, Y.A. Preliminary risk assessment of potential exposure to naturally occurring estrogens from Beijing (China) market milk products. Food Chem. Toxicol. 71: 74–80. 2014.

COCHRAN, W.G. Simple random sampling. Sampling Techniques. John Wiley and Sons, 3rd. Ed. NY. USA. Pp 442. 1997.

COMMISSION OF THE EUROPEAN COMMUNITIES. Commission directive 2013/46/EU of 28 august 2013 amending directive 2006/141/EC with regard to protein requirements for infant formulae and follow-on formulae follow-on formulae Text with EEA relevance. Of. J. Eur. Commun L 230. Online: https://bit.ly/3K96cUa. 11/11/2022.

COURANT, F.; ANTIGNAC, J.P.; MAUME, D.; MONTEAU, F.; ANDRE, F.; LE BİZEC, B. Determination of naturally occurring oestrogens and androgens in retail samples of milk and eggs. Food Additiv. Contamin. 24(12): 1358–1366. 2007.

FARLOW, D.W.; XIA, X.; TIMOTHY, D.V. Quantitative measurement of endogenous estrogen metabolites, risk-factors for development of breast cancer, in commercial milk products by LC–MS/MS. J. Chromatogr. B. 877(13): 1327–1334. 2009.

FITZ-GERALD, R.J.; MURRAY, B.A.; WALSH, D.J. Hypotensive peptides from milk proteins. J. Nutr. 134(4): 980–988. 2004.

FOOD AND DRUG ADMINISTRATION (FDA). Guidance for Industry: General Principles for Evaluating the Safety of Compounds Used in Food-Producing Animals; U.S. Food and Drug Administration: Washington, DC, USA. Pp 1–42. 2006.

FRIGGENS, N.C.; CHAGUNDA, M.G. Prediction of the reproductive status of cattle on the basis of milk progesterone measures: model description. Theriogenol. 64(1): 155–190. 2005.

GANMAA, D.; LI X.M., QIN L.Q.; WANG, P.Y.; TAKEDA, M.; SATO, A. The experience of Japan as a clue to the etiology of testicular and prostatic cancers. Med. Hypoth. 60(5): 724–730. 2003.

GANMAA, D.; SATO, A. The possible role of female sex hormones in milk from pregnant cows in the development of breast, ovarian and corpus uteri cancers. Med. Hypoth. 65(6): 1028–1037. 2005.

GAO, X.; LAVALLEY, M.; TUCKER, L. Prospective studies of dairy product and calcium ıntakes and prostate cancer risk: a meta-analysis. J. National Cancer Instit. 97(23): 1768–1777. 2005.

GINTHER, O.J.; NUTI, L.C.; GARCIA, M.C.; WENTWORTH, B.C.; TYLER, W.J. Factors affecting progesterone concentration in cow's milk and dairy products. J. Anim. Sci. 42(1): 155–159. 1976.

HARTMANN, S.; LACORN, M.; STEINHART, H. Natural occurrence of steroid hormones in food. Food Chem. 62(1): 7–20. 1998.

HIRPESSA, B.B.; ULUSOY, B.H.; HECER, C. Hormones and hormonal anabolics: residues in animal source food, potential public health impacts, and methods of analysis. J. Food Quality. 2020 (3): 1–12. 2020. https://doi.org/jw32.

HOFFMANN, B.; HAMBURGER, R.; KARG, H. Natural occurrence of progesterone in commercial milk products (author's transl). Z Lebensm Unters Forsch. 158(5): 257–259. 1975.

JOINT FAO/WHO EXPERT COMMİTTEE ON FOOD ADDITIVES (JECFA). Summary and Conclusions of the Fifty-second Meeting on Joint Expert Committee on Food Additives. JECFA. Rome, 02/16-18 Italy. Pp. 1–24. 1999.

JOUAN, P.N.; POULIOT, Y.; GAUTHIER, S.F.; LAFOREST, J.P. Hormones in bovine milk and milk products: A survey. Internat. Dairy J. 16: 1408–1414. 2006.

KALKAN, C.; ÖCAL, H. Erkek üreme fizyolojisi. Üreme fizyolojisi. Çiftlik Hayvanlarında Doğum ve Jinekoloji. Kaymaz, M.; Fındık, M.; Rişvanlı, A.; Köker, A. (Eds.). Medi Press, Malatya, Türkiye. Pp 13–44. 2015.

KAPLOWITZ, P.B. Pubertal development in girls: secular trends. Curr. Opinion Obstetr. Gynecol. 18: 487–491. 2006.

KAPLOWITZ, P.B. Link between body fat and the timing of puberty. Pediatr. 121: 208-217. 2008.

KHANIKI, J.G.R. Chemical Contaminants in milk and public health concerns: A review. Internat. J. Dairy Sci. 2: 104–115. 2007.

LOPEZ, H.; BUNCH, T.D.; SHIPKA, M.P. Estrogen concentrations in milk at estrus and ovulation in dairy cows. Anim. Reprod. Sci. 72(1–2): 37–46. 2002.

MARUYAMA, K.; OSHIMA, T.; OHYAMA, K. Exposure to exogenous estrogen through intake of commercial milk produced from pregnant cows. Pediatr. Internat. 52: 33–38. 2010.

MELNIK, B.C. Milk–the promoter of chronic Western diseases. Med. Hypoth. 72: 631–639. 2009.

NILI-AHMADABADI, A.; REZAEI, F.; HESHMATI, A.; RANJBAR, A.; LARKI-HARCHEGANI, A. Steroid hormone exposure as a potential hazard in milk consumers: a significant health challenge in Iran. J. Food Quality. 2021: 1–6. 2021.

PANDEY, A.K.; GHUMAN, S.P.S.; DHALIWAL, G.S.; HONPARKHE, M.; PHOGAT, J.B.; KUMAR, S. Effects of preovulatory follicle size on estradiol concentrations, corpus luteum diameter, progesterone concentrations and subsequent pregnancy rate in buffalo cows (Bubalus bubalis). Theriogenol. 107: 57–62. 2018.

PAPE-ZAMBITO, D.A.; MAGLIARO, A.L.; KENSINGER, R.S. 17β-Estradiol and Estrone Concentrations in Plasma and Milk During Bovine Pregnancy. J. Dairy Sci. 91: 127–135. 2007. https://doi.org/dkkhh9.

PAPE-ZAMBITO, D.A.; MAGLIARO, A.L.; KENSINGER, R.S. Concentrations of 17β-estradiol in Holstein whole milk. J. Dairy Sci. 90(7): 3308–3313. 2007.

PAPE-ZAMBITO, D.; ROBERTS, R.F.; KENSINGER, R.S. Estrone and 17β-estradiol concentrations in pasteurized-homogenized milk and commercial dairy products. J. Dairy Sci. 93: 2533–2540. 2010.

PETER, P.; SOUMYA, M.; SUBBAREDDY, V. Steroids in the milk we drink can lead to infertility, premature puberty and major health problems in humans. Internat. J. Med. Res. Pharmaceut. Sci. 6(11): 19–26. 2019.

REGAL, P.; DÍAZ-BAO, M.; BARREIRO, R.; CEPEDA, A.; FENTE, C. Occurrence of hormones in food of animal origin: new techniques of animal production and hormonal levels in dairy products. Proceedings of the EuroResidue VII Conference. Veldoben, 14-16 May, Netherlands. Pp 807-815. 2012.

REMESAR, X.; TANG, V.; FERRER, E.; TORREGROSA, C.; VIRGILI, J.; MASANES, R.M.; FERNANDEZ-LOPEZ, J.A.; ALEMANY, M. Estrone in food: a factor influencing the development of obesity?. Eur. J. Nutr. 38(5): 247–253. 1999.

SEBASTIAN, R.S.; GOLDMAN, J.D.; WILKINSON, E. C. Fluid Milk Consumption in the United States: What We Eat In America, NHANES 2005-2006. Food Surveys Research Group Dietary Data Brief No. 3. Pp 58. 2010.

SNOJ, T.; MAJDIČ, G.; KOBAL, S.; ŽUŽEK, M.; ČEBULJ-KADUNC, N. Estrone, 17b-estradiol and progesterone concentrations in processed milk with different fat content. Vet. Glasnik. 71(1): 35–43. 2017.

SCHWALM, J.W.; TUCKER, H.A. Glucocorticoids in mammary secretions and blood serum during reproduction and lactation and distributions of glucocorticoids, progesterone, and estrogens in fractions of milk. J. Dairy Sci. 61: 550–560. 1978.

SONI, N.; PANDEY, A.K.; KUMAR, A.; VERMA, A.; KUMAR, S.; GUNWANT, P.; SINGH, V. Expression of MTNR1A, steroid (ERα, ERβ, and PR) receptor gene transcripts, and the concentration of melatonin and steroid hormones in the ovarian follicles of buffalo. Dom. Anim. Endocrinol. 72: 106371. 2020.

STATİSTİCAL PACKAGE İN SOCİAL SCİENCES (SPSS). SPSS 22.0. Statistical package in social sciences for windows. Chicago, USA. 2015.

SÜMBÜLOĞLU, K.; SÜMBÜLOĞLU, V. Normal Dağılıma Uygunluk Testi. Biyoistatistik. 9. Baskı, Hatipoğlu Yayınları, Ankara, Türkiye. Pp 76–156. 2007.

VADGAMA, J.V.; WU, Y.; DATTA, G.; KHAN, H.; CHILLAR, R. Plasma insulin-like growth factor-I and serum IGF-binding protein 3 can be associated with the progression of breast cancer, and predict the risk of recurrence and the probability of survival in African-American and Hispanic women. Oncol. 57 (4): 330–340. 1999.

VICINI, J.; ETHERTON, T.; KRIS-ETHERTON, P.; BALLAM, J.; DENHAM, S.; STAUB, R.; GOLDSTEIN, D.; CADY, R.; MCGRATH, M.; LUCY, M. Survey of retail milk composition as affected by label claims regarding farm-management practices. J. Amer. Dietetic Ass. 108: 1198–1203. 2008.

VURAL, R.; GÜZELOĞLU, A.; KÜPLÜLÜ, Ş. Etçi ineklerde verimliliği en çok etkileyen postpartum sorunların Üreme fizyolojisi. Çiftlik Hayvanlarında Doğum ve Jinekoloji. Kaymaz, M.; Fındık, M.; Rişvanlı, A.; Köker, A. (Eds.). Medi Press. Malatya, Türkiye. Pp 107–131. 2015.

WOLFORD, S.T.; ARGOUDELIS, C.J. Measurement of estrogens in cow's milk, human milk, and dairy products. J. Dairy Sci. 62: 1458–1463. 1979.

YANG, Y.; LI, H.; ZHANG, J.; SUN, N.; SUN, H. Analysis of infant formula for steroid hormones by gas chromatography-tandem mass spectrometry using microwave-assisted extraction and gel permeation chromatography clean up. Food Anal. Meth. 7: 798–805. 2014.

Publicado
2023-02-21
Cómo citar
1.
Şeker P, Rişvanlı A, Şeker İbrahim, Çalıcıoğlu M. Determinación de los niveles de 17-β estradiol y progesterona en leche de vaca y leche de continuación para bebés mediante ELISA. Rev. Cient. FCV-LUZ [Internet]. 21 de febrero de 2023 [citado 20 de abril de 2024];33(1):1-. Disponible en: https://produccioncientificaluz.org/index.php/cientifica/article/view/39773
Sección
Salud Pública Veterinaria