Eficacia de BACTI-NIL®AQUA en infección experimental con Vibrio parahaemolyticus en juveniles de Litopenaeus vannamei

• María Soledad Morales-Covarrubias Center for Research in Food and Development A.C., Mazatlán Unit in Aquaculture and Environmental Management. Sinaloa, México
• María del Carmen Bolan-Mejía Center for Research in Food and Development A.C., Mazatlán Unit in Aquaculture and Environmental Management. Sinaloa, México
• Noemi Gracía-Aguilar Center for Research in Food and Development A.C., Mazatlán Unit in Aquaculture and Environmental Management. Sinaloa, México
• María-Mercè Isern-Subich ADISSEO. Antony, France
• Gilberto Hernández-González ADISSEO. Antony, France
• Waldo Gabriel Nuez-Ortín ADISSEO. Antony, France

DOI: https://doi.org/10.52973/rcfcv-e32148

Palabras clave: BACTI-NIL®AQUA, Vibrio parahaemolyticus, Penaeus vannamei, enfermedad de la necrosis hepatopancreática aguda, síndrome de la mortalidad temprana

Resumen

El objetivo de esta investigación fue evaluar la eficacia de dos dosis de BACTI-NIL^®AQUA adicionada al alimento en una infección experimental de 24 horas (h) en juveniles de Penaeus vannamei. Los resultados de este estudio mostraron que BACTI-NIL^®AQUA, a concentraciones de 3.000 y 5.000 partes por millón (ppm) inhibe el crecimiento de Vibrio parahaemolyticus (cepa M0904AHPND+), causante de la enfermedad de necrosis hepatopancreática aguda en camarones. En estas concentraciones, se observaron zonas de crecimiento de 15,00 ± 0,50 milímetros (mm) para 3.000 ppm y 17,00 ± 0,30 mm para 5.000 ppm, respectivamente. Se obtuvo una sobrevivencia del 60% para ambas dosis, el doble de sobrevivencia que el control positivo 13,33% (4 organismos) a las 24 h post-infección. En los organismos control positivo se observaron alteraciones histopatológicas en los túbulos del hepatopáncreas con desprendimiento celular severo e infiltración hemocítica dentro del tejido conectivo intertubular. En los organismos tratados con BACTI-NIL^®AQUA solo se observaron estructuras vermiformes en el lúmen de los túbulos del hepatopáncreas. Con los resultados del presente estudio se puede concluir que BACTI-NIL^®AQUA adicionado al alimento es una alternativa prometedora para el control de V. parahaemolyticus en cultivo de camarón.

Citas

ADAMS, D.; BOOPATHY, R. Use of formic acid to control vibriosis in shrimp aquaculture. Biol. 68(6): 1017-1021. 2013.

ANUTA, J.D.; BUENTELLO, A.; PATNAIK, S.; LAWRENCE, A.L.; MUSTAFA, A.; HUME, M.E. Effect of dietary supplementation of acidic calcium sulfate (Vitoxal) on growth, survival, immune response and gut microbiota of the Pacific white shrimp, Litopenaeus vannamei. J. World Aquac. Soc. 42(6): 834-844. 2011.

BAUER, A.W.; KIRBY, W.M.M.; SHERRIS, J.C.; TURCK, M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45: 493-496.1966.

BELL, T.A.; LIGHTNER, D.V. A handbook of normal penaeid shrimp histology. World Aquaculture Society. 595.3843. Pp 42-70. 1998.

BOLIVAR, N.C.; CHAMORRO-LEGARDA, E.; QUADROS-SEIFFERT, W.; ROBERTO-ANDREATTA, E.; DONASCIMENTO-VIEIRA, F. Combining a probiotic with organic salts presents synergistic in vitro inhibition against aquaculture bacterial pathogens. Braz. Arch. Biol. Technol. 61: 1-12. 2018.

CHAVEERACH, P.; KEUZENKAMP, D.A.; URLINGS, H.A.P.; LIPMAN, L.J.A.; VAN – KNAPEN, F. In vitro study on the effect of organic acids on Campylobacter jejuni/coli populations in mixtures of water and feed. Poult. Sci. 81: 621-628. 2002.

CHUCHIRD, N.; RORKWIREE, P.; RAIRAT, T. Effect of dietary formic acid and astaxanthin on the survival and growth of Pacific white shrimp (Litopenaeus vannamei) and their resistance to Vibrio parahaemolyticus. Springer Plus. 4 (440): 1-12. 2015.

CELIKEL, N; KAVAS, G. Antimicrobial properties of some essential oils against some pathogenic microorganisms. C. J. Food. Sci. 26: 174-181. 2008.

DEFOIRDT, T.; HALET, D.; SORGELOOS, P.; BOSSIER, P.; VERSTRAETE, W. Short-chain fatty acids protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. Aquac. 261(2): 804-808. 2006.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS (FAO). The State of World Fisheries and Aquaculture 2020. Sustainability in action. Rome. https://doi.org/gjkfd5.

GOEL, M.K.; KHANNA, P.; KISHORE, J. Understanding survival analysis: Kaplan-Meier estimate. Int. J. Ayurveda Res. 1: 274−278. 2010.

HONG, X.P.; XU, D.; ZHUO, Y.; LIU, H.Q.; LU, L.Q. Identification and pathogenicity of Vibrio parahaemolyticus isolates and immune responses of Penaeus vannamei (Boone). J. Fish. Dis. 39: 1085-1097. 2016.

HOSE, J.E.; MARTIN, G.G. Defense functions of granulocytes in the ridgeback prawn Sicyonia ingentis. J. Invertebr. Pathol. 53: 335–346. 1989.

JOHANSSON, M.; SÖDERHÄLL, K. Cellular Immunity in Crustaceans and the proPO System. Parasitol. Today. 5(6): 171-176. 1989.

LAMBERT, R.J.; SKANDAMIS, P.N.; COOTE, P.J.; NYCHAS, G.J. A study of the minimum inhibitory concentration and mode of action of oregano (Origanum vulgare ) essential oil, thymol and carvacrol. J. Appl. Microbiol. 91(3): 453-462. 2001.

LIGHTNER, D. V. Shrimps Diseases. A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp. 1st. Ed. World Aquaculture Society, Baton Rouge, LA. Pp 25-59. 1996.

LE MOULLAC, G.; SOYEZ, C.; SAULNIER, D.; ANSQUER, D.; AVARRE, J. C.; LEVY, P. Effect of hipoxic stress on the inmune response and the resistance to vibriosis of the shrimp Penaeus stylirostris in relation with moult cycle, protection against vibriosis. Fish Sellfish Immunol. 8: 621-629. 1998.

MANN, C.M.; MARKHAM, J.L. A new method for determining the minimum inhibitory concentration of essential oils. J. Appl. Microbiol. 84: 538-544. 1998.

MAURANDI-LÓPEZ, A.; BALSALOBRE R, C.; RÍO-ALONSO, L. Fundamentos estadísticos para investigación. 2013. En línea: https://bit.ly/3PvT2Ak. 10/01/2022.

MCDERMOTT, P.F.; BODEIS-JONES, S.M.; FRITSCHE; T.R., JONES, R.N.; WALKER, R.D. Broth microdilution susceptibility testing of Campylobacter and the determination of quality control ranges for fourteen antimicrobial agents. J. Clin. Microbiol. 43: 6136-6138. 2005.

MCFARLAND, J. Nephelometer: an instrument for media used for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J. Am. Med. Assoc. 14: 1176-1178. 1907.

MORALES-COVARRUBIAS, M.S.; GARCÍA-AGUILAR, N.; BOLAN-MEJÍA, M.D.; PUELLO-CRUZ, A.C. Evaluation of medicinal plants and colloidal silver efficiency against Vibrio parahaemolyticus infection in Litopenaeus vannamei cultured at low salinity. Dis. Aquat. Org. 122(1): 57-65. 2016.

MORALES-COVARRUBIAS, M.S.; GÓMEZ-GIL, B. Enfermedades bacterianas de camarones. Guía técnica-patología e inmunología de camarones penaeidos. OIRSA-Panamá. Pp.169-194. 2014.

MORALES-COVARRUBIAS, M.S. Los camarones peneidos. Enfermedades del camarón: detección mediante análisis en fresco e histopatología. Ed. TRILLAS. 2da. Ed. México. Pp. 5-130. 2010.

NETWORK OF AQUACULTURE CENTRES IN ASIA-PACIFIC (NACA). Acute hepatopancreatic necrosis disease card. Thailand. NACA. Pp. 2-5. 2014.

NETWORK OF AQUACULTURE CENTRES IN ASIA-PACIFIC (NACA). Report of the Asia Pacific emergency regional consultation on the emerging shrimp disease: Early mortality syndrome (EMS)/acute hepatopancreatic necrosis syndrome (AHPNS). Thailand. NACA. Pp. 9-10. 2012.

NG, W.K., LIM, C.L., ROMANO, N.; BKUA, B.C. Dietary short-chain organic acids enhanced resistance to bacterial infection and hepatopancreatic structural integrity of the giant freshwater prawn, Macrobrachium rosenbergii. Int. Aquat. Res. 9: 293-302. 2017.

NG, W.K.; KOH, B.C. The utilization and mode of action of organic acids in the feeds of cultured aquatic animals. Rev. Aquac. 9: 342-368. 2017.

NG, W.K.; KOH, B.C.; TEOH, C.Y.; ROMANO, N. Farm-raised shrimp, Penaeus monodon fed commercial feeds with added organic acids showed enhanced nutrient utilization, immune response and resistance to Vibrio harveyi challenge. Aquac. 449: 69–77. 2015.

ROMANO, N.; KOH, C.B.; NG, W.K. Dietary microencapsulated organic acids blend enhances growth, phosphorus utilization, immune response, hepatopancreatic integrity and resistance against Vibrio harveyi in white shrimp, Litopenaeus vannamei. Aquac. 435: 228-236. 2015.

SANTOS, H.M.; TSAI, C.Y.; MAQUILING, K.R.A.; TAYO, L.L.; MARIATULQABTIAH, A.R.; LEE, C.W.; CHUANG, K.P. Diagnosis and potential treatments for acute hepatopancreatic necrosis disease (AHPND): a review. Aquac. Inter. 28 (1): 169-185. 2020.

SONG, Y.L; HSIEH, Y.T. Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Dev. Comp. Immunol. 18: 201-209. 1994.

SOTO-RODRÍGUEZ, S.A.; GOMES-GIL, B.; LOZANO-OLVERA, R.; BETANCOURT-LOZANO, M.; MORALES-COVARRUBIAS, M.S. Field and experimental evidence of Vibrio parahaemolyticus as the causative agent of acute hepatopancreatic necrosis disease of cultured shrimp (Litopenaeus vannamei) in Northwestern Mexico. Appl. Environ. Microbiol. 81: 1689-1699. 2015.

SRIURAIRATANA, S.; BOONYAWIWAT, V.; GANGNONNGIW, W.; LAOSUTTHIPONG, C.; HIRANCHAN, J.; FLEGEL, T.W. White feces syndrome of shrimp arises from transformation, sloughing and aggregation of hepatopancreatic microvilli into vermiform bodies superficially resembling gregarines. Plos One. 9: 99-170. 2014.

THOMPSON, J.L.; HINTON, M. Antibacterial activity of formic and propionic acids in the diet of hens on salmonellas in the crop. Br. Poult. Sci. 38: 59-65. 1997.

TRAN, L.; NUNAN, L.; REDMAN, R.M.; MOHNEY, L.L.; PANTOJA, C.R.; FITZSIMMONS, K.; LIGHTNER, D.V. Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Dis. Aquat. Organ. 105: 45-55. 2013.

TRUSCOTT, R.; WHITE, K.N. The influence of metal and temperature stress on the immune system of crabs. Func. Ecol. 4: 455-461. 1990.

VAN DE BRAAK, C.B.T.; BOTTERBLOM, M.H.A.; TAVERNE, N.; VAN MUISWINKEL, W.B.; ROMBOUT, J.H.W.M.; VAN DE KNAAP, W.P.W. The roles of haemocytes and the lymphoid organ in the clearance of injected Vibrio bacteria in Penaeus monodon shrimp. Fish Shellfish Immunol.13: 239-309. 2002.

CLINICAL AND LABORATORY STANDARDS INSTITUTE (CLSI). M45: Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. 3rd. Ed. CLSI Guideline M45. Wayne. Pp. 3-19. 2016.

ZAR, J.H. Populations and simple. Biostatistical Analysis. 2nd. Ed. Prentice-Hall Inc. Englewood Cliffs. Pp. 7-18. 1984.