Effects of Xylazin on sedative, analgesic and some liver enzymes in rats
Abstract
This study was conducted to determine the sedation level of rats after using two different doses of Xylazine, to measure the damage that may occur in the liver, and to determine the change in pain response due to sedation. The use of analgesic substances is one of preferred methods in pain control studies. Analgesic effects are also present in drugs used for sedative purposes. Xylazine is used for pain relief, calming (sedative), sleep inducing (hypnotic), and striated muscle relaxation. It is also used for premedication purposes before using different anesthetic drugs. 24 rats used in the study were randomly selected and divided into three groups. Only physiological saline was given to the rats in the control group (KO). The second group was given Xylazine (K10) 10 mg/kg intraperitoneally (ip), and the third group was given Xylazine (K15) 15 mg/kg/ip. Sedation assessment was performed according to the behavior of the rats to which sedative drugs were applied to the stimuli. Sedation was classified as mild, moderate and severe. The degree of pain-relieving properties of Xylazine in rats was measured with a pain test. The pain test was performed with a hotplate test and a hotplate apparatus. After the pain tests, the rats were sacrificed after taking blood as needed. AST, ALT and corticosteroid values were measured in the blood serum. In this study, it was determined that Xylazine had pain-relieving properties and did not change liver enzyme values when used at a dose of 15 mg/kg in rats, and it was concluded that it could be preferred for safe sedation in cases where short-term complete immobility was needed.
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Wagner MC, Hecker KG ,Pang DSJ. Sedation levels in dogs: a validation study. BMC Vet. Res. [Internet]. 2017[cited December 24 2024]; 13:110. Available in: https://goo.su/5HKUTeQ DOI: https://doi.org/10.1186/s12917-017-1027-2
Ünsaldı S. Veteriner Anestezi. Birinci Baskı. İstanbul: Nobel Tıp Kitapevleri; 2011. p. 28-66.
Rioja E, Santos M, Martínez-Taboada F, AIbancovichi JA, Tendillo FJ. Cardiorespiratory and minimum alveolar concentration sparing effects of a continuous intravenous infusion of dexmedetomidine in halothane or isoflurane- anaesthetized rats. Lab. Anim. [Internet]. 2006; 40(1):9-15. doi: https://doi.org/fcn8v5 DOI: https://doi.org/10.1258/002367706775404363
Buerkle H, Yaksh TL. Pharmacological evidence for different alpha 2-adrenergic receptor sites mediating analgesia and sedation in the rat. Br. J. Anaesth. [Internet]. 1998; 81(2):208-215. doi: https://doi.org/pfw8 DOI: https://doi.org/10.1093/bja/81.2.208
Guo TZ, Jiang JY, Buttermann AE, Maze M. Dexmedetomidine injection into the locus ceruleus produces antinociception. Anesthesiology. [Internet]. 1996; 84(4):873-881. doi: https://doi.org/cw4ftz DOI: https://doi.org/10.1097/00000542-199604000-00015
Topal A. Veteriner Anestezi. Birinci Baskı. Bursa: Nobel Tıp Kitapevleri; 2005. p. 41-48.
Raj PP. Ağrı taksonomisi. In: Erdine S, editor. Ağrı. Birinci baskı. İstanbul: Alemdar Ofset; 2000.
Merskey H, Bogduk N. Descriptions of chronic pain syndromes and definition of pain terms, Seattle: IASP Press; 1994
Kartal F, Özyalçın NS. Hayvanlarda akut ve kronik ağrı modellerinin değerlendirilmesi. In: Önal A, editor. Algoloji. Birinci Baskı. İstanbul: Nobel Tıp Kitabevi; 2004. p. 299-324.
Bennett GJ. Animal models and their clinical implications. In: Merskey H, Loeser JD, Dubner R, (ed). The Paths of Pain 1975-2005. Seattle: IASP Press; 2005. p. 243-251
Papudesi BN, Malayala SV, Regina AC. Xylazine Toxicity. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025[cited Jul. 17 2024]. Available in: https://goo.su/WBuPM1M
Koç B, Sarıtaş K. Veteriner Anesteziyoloji ve Reanimasyon. Ankara: Medipres yayınevi; 2004. p. 80-94.
Karasu A, Gençcelep M. The Effect of Xylazine HCl Used in Repeated Sedations for Sheep on Biochemical and Clinical Values. Kafkas Univ Vet Fak Derg. [Internet]. 2015; 21(6):831-836. doi: https://doi.org/pfxc
Carvalho LL, Nishimura LT, Borges LP, Cerejo SA, Villela IO, Auckburally A, de Mattos- Junior E. Sedative and cardiopulmonary effects of xylazine alone or in combination with methadone, morphine or tramadol in sheep. Vet. Anaesth. Analg. [Internet]. 2016; 43(2):179-188. doi: https://doi.org/f8j6fp DOI: https://doi.org/10.1111/vaa.12296
Guerri G, Cerasoli I, Straticò P, De Amicis I, Giangaspero B, Varasano V, Paolini A, Carluccio A, Petrizzi L. The Clinical Effect of Xylazine Premedication in Water Buffalo Calves (Bubalus bubalis) Undergoing Castration under General Anaesthesia. Animals. [Internet]. 2021; 11(12):3433. doi: https://doi.org/pfxd DOI: https://doi.org/10.3390/ani11123433
Samimi A. Evaluation of the sedative and clinical effects of xylazine, detomidine, medetomidine and dexmedetomidine in miniature donkeys. NZVJ. [Internet]. 2019; 68(3):198–202. doi: https://doi.org/pfxg DOI: https://doi.org/10.1080/00480169.2019.1698998
Jansson T, Perera BV, Edner A, Fahlman A. Standing sedation with xylazine and reversal with yohimbine in juvenile asian elephants (Elephas maximus). J. Zoo. Wild. Med. [Internet]. 2021; 52(2):437-444. doi: https://doi.org/gkkcwm DOI: https://doi.org/10.1638/2020-0170
Morgan GE, Mikhail MG . Pain Management. In: Clinical Anesthesiology, 2nd ed. New Jersey: Prenttice-Hall Interntional, Inc; 1996. p. 274-316.
Cousins M, Power I. Acute and postoperative pain. In: Wall PD, Melzack R, editors. Textbook of Pain. 4th ed. London. Churchill Livingstone; 1999. p. 447-491.
Gürel ÖK. Değişik Muskuloskeletal Sistem Hastalıklarının Basınç Ağrı Eşiği Üzerine Etkisi [specialization thesis on the Internet]. İstanbul: Haydarpaşa Numune Eğitim ve Araştırma Hastanesi Fiziksel Tıp Ve Rehabilitasyon Kliniği; 2009. [Accessed December 23 2024]. Available in: https://goo.su/dFDq
Keskinbora K, Aydınlı I. An atypical opioid analgesic: Tramadol. Pain. [Internet]. 2006 [Accessed 23 December 2024]; 18(1):5-19. Available in: https://goo.su/za7Kua
Grond S, Sablotzki A. Clinical pharmacology of tramadol. Clin. Pharmacokinet. [Internet]. 2004; 43(13):879-923. doi: https://doi.org/dq5s8c DOI: https://doi.org/10.2165/00003088-200443130-00004
Uçkunkaya N, Gurbet A. Baş ve yüz ağrıları. In: Güldoğuş F, Güleç S, Uçkunkaya N, editörler. Ağrı fizyolojisi. Birinci Baskı. Ankara: Güneş Kitabevi; 2007. p. 1-11.
Treede RD, Jensen TS, Campbell JN, Cruccu G, Dostrovsky JO, Griffin JW, Hansson P, Hughes R, Nurmikko T, Serra J. Neuropathic pain: redefinition and a grading system for clinical and research purposes. Neurology. [Internet]. 2008; 70(18):1630–1635. doi: https://doi.org/bgm4bw DOI: https://doi.org/10.1212/01.wnl.0000282763.29778.59
Köknel Talu G. Nöropatik Ağrı. In: Erdine S, editor. Ağrı. Üçüncü Baskı. İstanbul: Nobel Tıp Kitabevi; 2008. p. 461-471.
Jang HS, Lee MG. Effects of medetomidine on analgesia and sedation in rats. J Vet Clin. [Internet]. 2010[Accessed December 23 2024]; 27(6):674-678. Available in: https://goo.su/2t2ex0
Gençcelep M, Karasu A. Evaluation of analgesic and sedative effects of repeated and increasing doses of xylazine HCl in sheep. Med. Weter. [Internet]. 2017; 73(8):468-472. doi: https://doi.org/pfxk DOI: https://doi.org/10.21521/mw.5760
Edinoff A, Sall S, Upshaw W, Spillers N, Vincik LA, Witt A, Murnane K, Kaye Adam and Kaye Alan. Xylazine: A Drug Adulterant of Clinical Concern. Current Pain and Headache Reports. [Internet]. 2024; 28(5): 417-426. doi: https://doi.org/pfxm DOI: https://doi.org/10.1007/s11916-024-01211-z
Gomes VH, Monterio ER, Dias RS, de Olivera RLS, Alburquerque-da Silva MF, Coelho K. Comparison of the sedative effects of morphine, meperidine or fentanyl, in combination with acepromazine, in dogs. Cienc. Rural. [Internet]. 2011; 41(8):1411-1416. doi: https://doi.org/c5598q DOI: https://doi.org/10.1590/S0103-84782011005000102
Bell AM, Auckburally A, Pawson P, Scott EM, Flaherty D. Two doses of dexmedetomidine in combination with buprenorphine for premedication in dogs; a comparisonwith acepromazine and buprenorphine. Vet. Anaesth. Analg. [Internet]. 2011; 38(1):15-23. doi: https://doi.org/d527nq DOI: https://doi.org/10.1111/j.1467-2995.2010.00576.x
Hassan MA, Abd-Elkawi M, Saber MS, Senosy W, Nassif M, ElSherif MW. Evaluation of Xylazine Ketamine Anesthesia in Rabbits Undergoing Tendon Surgery: A Prospective Randomized Controlled Study. NVVJ. [Internet]. 2024; 4(1):1-7. doi: https://doi.org/pfxs DOI: https://doi.org/10.21608/nvvj.2023.247402.1038
Bilgili A, Altıntaş L, Şahindokuyucu F. The use of Sedative and Immobilizan Drugs in Cats and Dogs. YYU Vet Fak Derg. [Internet]. 2003 [Accessed December 22 2024]; 14(1):77-82. Available in: https://goo.su/cTzCiUy
Fayed AH, Zakaria AD, Hedaya SA, El-Ashmawy IM. Effect xylazine (alpha 2-adrenergic agonist) on the stress response to immobilization and heat in rats. AJAS. [Internet]. 1994[Accessed December 23 2024]; 7(3):397-400. Available in: https://goo.su/g6A5dS DOI: https://doi.org/10.5713/ajas.1994.397
Amin S, Wagner R, Fieseler H, Rizk A, Schmicke M, Spilke J, Mielenz N, Baumgartner W, Schären-Bannert M and Starke A. Sedation with xylazine hydrochloride decreases the stress response in merino meat sheep during routine hoof trimming in a tilt table. Front. Anim. Sci. [Internet]. 2022; 2:795543. doi: https://doi.org/pfxx DOI: https://doi.org/10.3389/fanim.2021.795543
Mert N. Veteriner Klinik Biyokimya. 1th. ed.Baskı. İstanbul: Ceylan Matbaacılık;1997. p. 230-240.
Dolar E. Klinik Karaciğer Hastalıkları. 1th. ed.Baskı. Ankara: Nobel&Güneş Tıp Kitabevi; 2002. p. 133-146.
Guyton AC, Hall JE. Tıbbi Fizyoloji. 12th. ed. Baskı. İstanbul: Nobel Tıp Kitapevi; 2013. p. 937- 942.
Ersoy O. Evaluation of elevated liver enzymes. Ankara Med J. [Internet]. 2012 [Accessed December 22 2024]; 12(3):129-135. Available in: https://goo.su/wBoq2ya
