Histomorphometric changes and colonisation level of Lactobacillus acidophillus and Kluyveromyces fragilis in different digestive tract segments of Guinea pigs (Cavia porcellus)

• José Miranda–Yuquilema Universidad Nacional de Chimborazo, Facultad de Ingeniería, Grupo de Producción Animal e Industrialización. Riobamba, Ecuador
• Juan Taboada–Pico Universidad de Cuenca, Facultad de Ciencias Agropecuarias. Cuenca, Ecuador
• Wilfido Briñez–Zambrano Universidad del Zulia, Facultad de Ciencias Veterinarias. Maracaibo, Venezuela
• Mercy Cuenca–Condoy Universidad Católica de Cuenca, Facultad de Ciencias Veterinarias. Cuenca, Ecuador

DOI: https://doi.org/10.52973/rcfcv-e34484

Keywords: Gut microbiota, probiotics, health, intestinal villi

Abstract

The aim of the study was to evaluate the action of probiotic bioadditives on histomorphometric changes and colonization levels in different segments of the digestive tract in 90–day–old guinea pigs. Eighty Kuri guinea pigs of 30 d of age, 250 g live weight were randomly selected and distributed into four groups of 20 animals each. Ctrl, Control. Bal, vinasse–molasses substrate fermented with Lactobacillus acidophilus. Lev, vinasse–molasses substrate fermented with Kluyveromyces fragilis. B+L, vinasse–molasses substrate fermented with L. acidophilus and K. fragilis. Bal, Lev and B+L contained molasses–vinasse substrate in their base. The parameters evaluated were macroscopic lesions in the digestive tract organs, morphometric changes and microbiota of the digestive tract. The organs of the digestive tract in guinea pigs of the control group presented a greater (P<0.05) amount of macroscopic lesions; the samples of the intestine, duodenum, jejunum and ilium of the animals that consumed probiotics presented a greater (P<0.05) length; similar situation presented the histomorphometric changes of intestinal tissues in guinea pigs with probiotics, the microbial load detected from the rectal swab in the different culture media with significant statistical differences (P<0.05). It is concluded that the inclusion of microbial additive in guinea pigs influences morphometric changes, especially in the length and width of intestinal villi, crypt depth and length/depth ratio at 45 and 90 d of age. It was also possible to verify the level of colonization carried out by the strains of beneficial microorganisms in different segments of the digestive tract.

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