Bacterial susceptibility profile in Staphylococcus spp. strains isolated from canines with otitis externa

• Robert Gustavo Sánchez–Prado Universidad Técnica de Machala. Machala, El Oro, Ecuador - Universidade Federal Do Pará, Programa de Pós–Graduação em Saúde Animal na Amazônia. Castanhal, Brasil
• Joseline Paola Santo–Endara Universidad Técnica de Machala. Machala, El Oro, Ecuador
• Raquel Estefania Sánchez–Prado Universidade Federal Do Pará, Programa de Pós–Graduação em Saúde Animal na Amazônia. Castanhal, Brasil
• Samantha Guzmán–Pucha Universidad Técnica de Machala. Machala, El Oro, Ecuador
• Fernando Lenin Aguilar–Gálvez Universidad Técnica de Machala. Machala, El Oro, Ecuador
• Lorena Elizabeth Chalco–Torres Universidad Técnica de Machala. Machala, El Oro, Ecuador
• Dioselina Esmeralda Pimbosa–Ortiz Universidad Técnica de Machala. Machala, El Oro, Ecuador
• Jhonny Edgar Pérez–Rodriguez Universidad Técnica de Machala. Machala, El Oro, Ecuador

DOI: https://doi.org/10.52973/rcfcv-e34364

Keywords: Staphylococcus spp., bacterial resistance, antibiotics, ear microbiota, otitis

Abstract

External otitis is an inflammation of the external auditory canal, which generally affects canines, its causes can be multiple. The objective of this research was to determine the bacterial susceptibility profile in Staphylococcus spp. strains, present in canines affected by otitis externa. The methodology initially used was the identification of affected canines, taking into account their signs and symptoms (pain on palpation, erythema, edema, hypersecretion, pruritus, edema), then a sample of atrial secretion from one ear was taken from each one using a sterile swab, which were seeded on agar base with 5% defibrinated sheep blood, and incubated at 37°C for 24 hours. As results we obtained that of the 46 samples collected from patients with a history of external otitis, it was possible to isolate 65.21% of Staphylococcus spp. Regarding the sensitivity and resistance profile, the strains isolated in this study showed greater sensitivity to Gentamicin (100%), followed by Doxycillin (96.67%) and Cefoxitin (90%). Among the antimicrobials that presented the highest resistance in vitro were Erythromycin (26.66%), followed by Clindamycin (23.33%) and Levofloxacin (23.33). Considering the degree of resistance to families of antibiotics, Lincosamides (23.33%), Macrolides (21.66%), Fluoroquinolones (16.66%) developed greater resistance in vitro. Culture and antibiogram become a fundamental tool for successful antimicrobial therapy and avoid the development of multiresistant strains.

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