Physicochemical and structural properties of beef meat thawed using various methods
Abstract
Four meat thawing techniques that are most commonly used in daily life were used: refrigerator thawing, microwave thawing, ambient temperature thawing, and water thawing, to evaluate the physico-chemical and histological alterations in thawed beef. After thawing, the structural, chemical, and physical characteristics of beef meat were evaluated. The results showed that meat thawed in the refrigerator at 4°C was characterized by the highest pH value (5.65 ± 0.02) and a significant difference (P<0.05) compared to meat thawed by other thawing methods. Also for the electrical conductivity, it reached the highest value (1.442 ± 1,012) in the microwave oven (P<0.05); meanwhile, water activity decreased significantly after thawing regardless of the thawing method (P<0.05). On the other hand, refrigerator thawing resulted in the least amount of water loss (1.23%) with P<0.05, while high levels of microwave energy caused significant water loss, represented by thawing loss and cooking loss (4.37% and 44.47%), respectively, with P<0.05. Among different thawing methods, microwave thawing had the highest level of TBARS, with a mean of 0.25 ± 0.034 mg·kg-1 (P<0.05). Regarding the color, the lightness (L*) value in the microwave-thawed samples decreased significantly (P<0.05) compared to the fresh control. Histologically, samples that were thawed in a refrigerator preserved the integrity of the fibers' structure after thawing better than other methods; samples thawed in a microwave, however, caused more structural damage. To ensure that it thaws uniformly and to retain the meat's quality as close to its fresh quality as possible, it is typically advised to thaw meat in a slower, more gradual manner, such as in the refrigerator.
Downloads
References
ABLIKIM, B.; LIU, Y.; KERIM, A.; SHEN, P.; ABDURERIM, P.; ZHOU, G.H. Effects of breed, muscle type, and frozen storage on physico-chemical characteristics of lamb meat and its relationship with tenderness. CYTA – J. Food. 14(1): 109–116. 2016. https://doi.org/j724.
ALI, S.; ZHANG, W.; RAJPUT, N.; KHAN, M.A.; LI, C.B.; ZHOU, G.H. Effect of multiple freeze-thaw cycles on the quality of chicken breast meat. Food Chem. 173: 808–814. 2015. https://doi.org/j725.
BENLI, H. Consumer attitudes toward storing and thawing chicken and effects of the common thawing practices on some quality characteristics of frozen chicken. Asian-Australas J. Anim. Sci. 29(1): 100–108. 2016. https://doi.org/j726.
BLACK, J.L.; JACZYNSKI, J. Effect of water activity on the inactivation kinetics of Escherichia coli O157:H7 by electron beam in ground beef, chicken breast meat, and trout fillets. Int. J. Food Sci. Technol. 43(4): 579–586. 2008. https://doi.org/dhtrcv.
BOZZETTA, E.; MEISTRO, S.; PEZZOLATO, M.; MUSCOLINO, D.; GIARRATANA, F.; BAIONI, E.; PANEBIANCO, A. Histology as a valid and reliable tool to differentiate fresh from frozen-thawed fish. J. Food Prot. 75(8): 1536–1541. 2012. https://doi.org/f35nqk.
BUEGE, J.A.; AUST, S.D. Biomembranes – Part C: Biological Oxidations. Meth. Enzymol. 52: 302–310. 1978. https://doi.org/cgmt6k.
CHOI, E.J.; PARK, H.W.; CHUNG, Y.B.; PARK, S.H.; KIM, J.S.; CHUN, H.H. Effect of tempering methods on quality changes of pork loin frozen by cryogenic immersion. Meat Sci. 124: 69–76. 2017. https://doi.org/gppg4h.
CHOI, E.J.; PARK, H.W.; YANG, H.S.; KIM, J.S.; CHUN, H.H. Effects of 27.12 MHz radio frequency on the rapid and uniform tempering of cylindrical frozen pork loin (longissimus thoracis et lumborum. Korean J. Food Sci. Anim. Resour. 37(4): 518–528. 2017. https://doi.org/j727.
ERSOY, B.; AKSAN, E.; ÖZEREN, A. The effect of thawing methods on the quality of eels (Anguilla anguilla). Food Chem. 111(2): 377–380. 2008. https://doi.org/dqqdj6.
FUENTES, A.; MASOT, R.; FERNÁNDEZ-SEGOVIA, I.; RUIZ-RICO, M.; ALCAÑIZ, M.; BARAT, J.M. Differentiation between fresh and frozen-thawed sea bream (Sparus aurata) using impedance spectroscopy techniques. Innov. Food Sci. Emerg. Technol. 19: 210–217. 2013. https://doi.org/f46zj3.
HOU, Q.; CHENG, Y.P.; KANG, D.C.; ZHANG, W.G.; ZHOU, G.H. Quality changes of pork during frozen storage: comparison of immersion solution freezing and air blast freezing. Intern. J. Food Sci. Technol. 55(1): 109–118. 2020. https://doi.org/j728.
JIA, G.; SHA, K.; MENG, J.; LIU, H. Effect of high voltage electrostatic field treatment on thawing characteristics and post-thawing quality of lightly salted, frozen pork tenderloin. LWT. 99: 268–275. 2019. https://doi.org/j729.
KIM, H.W.; KIM, J.H.; SEO, J.K.; SETYABRATA, D.; KIM, Y.H.B. Effects of aging/freezing sequence and freezing rate on meat quality and oxidative stability of pork loins. Meat Sci. 139: 162–170. 2018. https://doi.org/gdfn99.
KIM, T.H.; CHOI, J.H.; CHOI, Y.S.; KIM, H.Y.; KIM, S.Y.; KIM, H.W.; KIM, C.J. Physicochemical properties of thawed chicken breast as affected by microwave power levels. Food Sci. Biotechnol. 20(4): 971–977. 2011. https://doi.org/fjwpmr.
KIM, Y.B.; JEONG, J.Y.; KU, S.K.; KIM, E.M.; PARK, K.J.; JANG, A. Effects of various thawing methods on the quality characteristics of frozen beef. Korean J. Food Sci. Anim. Resour. 33(6): 723–729. 2013. https://doi.org/j73b.
KONDRATOWICZ, J.; CHWASTOWSKA, I. Sensory quality of pork and total microbial count depending on deep-freeze storage time and thawing method. Vet. Zoot. 33(55): 43-46. 2006.
LAKEHAL, S.; BENNOUNE, O.; AYACHI, A. Investigation of the physico-chemical and microstructure changes of beef meat during frozen storage at -23°C. Meat Tech. 62(2): 121–129. 2021. http://doi.org/j73c.
LEYGONIE, C.; BRITZ, T.J.; HOFFMAN, L.C. Meat quality comparison between fresh and frozen/thawed ostrich M. iliofibularis. Meat Sci. 91(3): 364–368. 2012. https://doi.org/f3x6xv.
LEYGONIE, C.; BRITZ, T.J.; HOFFMAN, L.C. Impact of freezing and thawing on the quality of meat: Review. Meat Sci. 91(2): 93–98. 2012. https://doi.org/fzh375.
LI, B.; SUN, D.W. Novel methods for rapid freezing and thawing of foods – A review. J. Food Eng. 54(3): 175–182. 2002. https://doi.org/bsvzns.
LI, F.; WANG, B.; LIU, Q.; CHEN, Q.; ZHANG, H.; XIA, X.; KONG, B. Changes in myofibrillar protein gel quality of porcine longissimus muscle induced by its structural modification under different thawing methods. Meat Sci. 147: 108–115. 2019. https://doi.org/gjqtqg.
MEDIĆ, H.; KUŠEC, I.D.; PLEADIN, J.; KOZAČINSKI, L.; NJARI, B.; HENGL, B.; KUŠEC, G. The impact of frozen storage duration on physical, chemical and microbiological properties of pork. Meat Sci. 140: 119–127. 2018. https://doi.org/gdhwf9.
MEERSMAN, F.; DOBSON, C.M.; HEREMANS, K. Protein unfolding, amyloid fibril formation and configuration al energy landscapes under high pressure conditions. Chem. Soc. Rev. 35(10): 908–917. 2006. https://doi.org/dgsm7s.
MINZ, P.S.; SAINI, C.S. Comparison of computer vision system and colour spectrophotometer for colour measurement of mozzarella cheese. Appl. Food Res. 1(2): e100020. 2021. https://doi.org/j73f.
MIR, N.A.; RAFIQ, A.; KUMAR, F.; SINGH, V.; SHUKLA, V. Determinants of broiler chicken meat quality and factors affecting them: a review. J. Food Sci. Technol. 54(10): 2997–3009. 2017. https://doi.org/gbzkkx.
MUCHENJE, V.; DZAMA, K.; CHIMONYO, M.; STRYDOM, P.E.; HUGO, A.; RAATS, J.G. Some biochemical aspects pertaining to beef eating quality and consumer health: A review. Food Chem. 112(2): 279–289. 2009. https://doi.org/cjnj3v.
MUELA, E.; MONGE, P.; SAÑUDO, C.; CAMPO, M.M.; BELTRÁN, J.A. Meat quality of lamb frozen stored up to 21 months: Instrumental analyses on thawed meat during display. Meat Sci. 102: 35–40. 2015. https://doi.org/f64fd7.
OFFICIAL JOURNAL OF THE DEMOCRATIC AND POPULAR REPUBLIC OF ALGERIA. 2017. Executive Decree No. 17-140 setting the conditions of hygiene and sanitation during the process of putting foodstuffs for human consumption. Algeria. On Line: https://bit.ly/40I4v4E. 22/01/2023.
OLIVEIRA, M.R.; GUBERT, G.; ROMAN, S.S.; KEMPKA, A.P.; PRESTES, R.C. Meat quality of chicken breast subjected to different thawing methods. Rev. Bras. Cien. Avic. 17(2): 165–172. 2015. https://doi.org/j73g.
PARTANEN, K.; SILJANDER-RASI, H.; HONKAVAARA, M. Effects of finishing diet and pre-slaughter fasting time on meat quality in crossbred pigs. Agric. Food Sci. 16(3): 245–258. 2007. https://doi.org/bk6wjn.
PIETRASIK, Z.; JANZ, J.A.M. Influence of freezing and thawing on the hydration characteristics, quality, and consumer acceptance of whole muscle beef injected with solutions of salt and phosphate. Meat Sci. 81(3): 523–532. 2009. https://doi.org/b4cmqq.
QIAN, S.; LI, X.; WANG, H.; SUN, Z.; ZHANG, C.; GUAN, W.; BLECKER, C. Effect of sub-freezing storage (-6, -9 and -12°C) on quality and shelf life of beef. Intern. J. Food Sci. Technol. 53(9): 2129–2140. 2018. https://doi.org/gd5gbp.
SIGURGISLADOTTIR, S.; INGVARSDOTTIR, H.; TORRISSEN, O.J.; CARDINAL, M.; HAFSTEINSSON, H. Effects of freezing/thawing on the microstructure and the texture of smoked atlantic salmon (Salmo salar). Intern. J. Food Res. J. 33(10): 857–865. 2000. https://doi.org/fdzqzr.
SONALE, O.V.; CHAPPALWAR, A.M.; DEVANGARE, A.A. Effect of frozen storage on the physico-chemical quality and histology of quail breast meat. Indian. J. Anim. Res. 10: 426–435. 2014.
STRATEVA, M.; PENCHEV, G. Histological discrimination of fresh from frozen/thawed carp (Cyprinus carpio). Bulg. J. Vet. Med. 24(3): 434–441. 2021. https://doi.org/j73h.
VIEIRA, C.; DIAZ, M.T.; MARTÍNEZ, B.; GARCÍA-CACHÁN, M.D. Effect of frozen storage conditions (temperature and length of storage) on microbiological and sensory quality of rustic crossbred beef at different states of ageing. Meat Sci. 83(3): 398–404. 2009. https://doi.org/dthjs3.
WANG, B.; KONG, B.; LI, F.; LIU, Q.; ZHANG, H.; XIA, X. Changes in the thermal stability and structure of protein from porcine longissimus dorsi induced by different thawing methods. Food Chem. 316: e126375. 2020. https://doi.org/gmvbxq.
XIA, X.; KONG, B.; LIU, J.; DIAO, X.; LIU, Q. Influence of different thawing methods on physicochemical changes and protein oxidation of porcine longissimus muscle. Lwt. 46(1): 280–286. 2012. https://doi.org/brdh5g.
XIA, X.; KONG, B.; LIU, Q.; LIU, J. Physicochemical change and protein oxidation in porcine longissimus dorsi as influenced by different freeze-thaw cycles. Meat Sci. 83(2): 239–245. 2009. https://doi.org/bmg5mh.
ZHANG, M.; XIA, X.; LIU, Q.; CHEN, Q.; KONG, B. Changes in microstructure, quality and water distribution of porcine longissimus muscles subjected to ultrasound-assisted immersion freezing during frozen storage. Meat Sci. 151: 24–32. 2019. https://doi.org/gppg4j.
ZHANG, X.; GAO, T.; SONG, L.; ZHANG, L.; JIANG, Y.; LI, J.L.; GAO, F.; ZHOU, G.H. Effects of different thawing methods on the quality of chicken breast. Intern. J. Food Sci. Technol. 52(9): 2097–2105. 2017. https://doi.org/gbwpp9.
ZHU, M.M.; PENZ.Y.; LU, S.; HE, H.J.; KANG, Z.L.; MA, H.J.; ZHAO, S.M.; WANG, Z.R. Physicochemical properties and protein denaturation of pork longissimus dorsi muscle subjected to six microwave-based thawing methods. Foods. 9(1): 26. 2020. https://doi.org/j73j.
ZHU, Z.; ZHOU, Q.; SUN, D.W. Measuring and controlling ice crystallization in frozen foods: A review of recent developments. Trends Food Sci. Technol. 90: 13–25. 2019. https://doi.org/gpkcmf.
Copyright (c) 2023 Saliha Lakehal, Aicha Lakehal, Salima Lakehal, Omar Bennoune, Ammar Ayachi
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.