Characterization of Leptin gene polymorphisms in Carora sires
Caracterización de polimorfismos del gen leptina en sementales de la raza Carora
Keywords:
Allele, bovine, genotype, leptin, polymorphism
Abstract
The objective of this work was to characterize the LepClaI(rs29004487), LepKpn2I (rs29004488), LepHphI (rs29004508) and LepSau3AI (rs29004501) single nucleotide polymorphisms (SNP) of the Leptin gene in Carora sires. Blood and semen samples were collected from 43 bulls born between 2002 and 2013 for DNA extraction; this was obtained from salting out methodologies. Genotyping was carried out through the PCR-RFLP analysis and by an amplification system with four primers, refractory to mutations (TETRA PRIMER PCR). The amplification and digestion products were verified in horizontal electrophoresis in agarose gels. Allelic and genotypic frequencies, observed heterozygosity (Ho) were calculated, and Hardy-Weinberg equilibrium was tested. Allelic and genotypic frequencies were respectively 0.98(A), 0.02(T) and 0.95(AA), 0.05(AT) for rs29004487 polymorphism; 0.59(C), 0, 41(T) and 0.14(CC), 0.53(CT), 0.33(TT) for rs29004488; 0.8(C), 0.2(T) and 0.67(CC), 0.26(CT), 0.07(TT) for rs29004501; 0.94(C), 0.06(T) and 0.88(CC), 0.12 (CT) for rs29004508. No deviations from Hardy-Weinberg equilibrium were observed and the existence of linkage disequilibrium was evident among the majority of SNPs studied. The allelic and genotypic frequencies of the polymorphisms evaluated in the Carora bull population were similar to those obtained in other breeds. This first approach in the characterization of the Leptin gene in Carora cattle will allow to consider the genetic potential of the breed for the production of milk or meat.
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References
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LAGONIGRO, R.; WIENER, P.; PILLA, F.; WOOLLIAMS, J.A.; WILLIAMS, J.L. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Anim. Genet.. 34(5): 371-374. 2003
LEVENE, H. On a matching problem in genetics. Ann. Math. Statist. 20(1): 91-94. 1949
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LIEFERS, S.C.; VEERKAMP, R.F.; TE PAS, M.F.; DELAVAUD, C.; CHILLIARTL, M.; PLATJE, M.; VAN DER LENDE, T. Leptin promoter mutation affects leptin levels and performance traits in dairy cows. Anim. Genet. 36(2): 111-118. 2005
MARSON, E.P.; FERRAZ, J.B.; MEIRELLES, F.V.; BALIEIRO, J.C.; ELER, J.P.; FIGUEIREDO, L.G.; MOURAO, G.B. Genetic characterization of European Zebu composite bovine using RFLP markers. Genet. Molec. Res. 4(3): 496-505. 2005
MARTÍNEZ, R.; ROCHA, J.F.; BEJARANO, D.; GÓMEZ, Y.; ABUABARA, Y.; GALLEGO, J. Identification of SNPs in growth related genes in colombian creole cattle. Genet. Molec. Res.. 15(3): 1-16. 2016
METIN, J.; ARSLAN, K.; AKYUZ, B.; ALIBER, M.; GAMZE, E.; ULAS, M. Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. Int. J. Dairy Technol. 72(1): 1-7. 2019
MONTOYA, A.; CERÓN MUÑOZ, M.; TRUJILLO, E.; RAMIREZ, E.; ÁNGEL, P. Frecuencia de los marcadores del gen Leptina en razas bovinas criollas y colombianas: Romosinuano, Chino Satnadereano, Sanmartinero y Velásquez. Rev. Científ. FCV-LUZ. XIX(1): 38-48. 2009
MORAVCIKOVA, N.; TRAKOVICKA, A.; KASARDA, R. Polymorphism within the Intron Region of the bovine Leptin Gene in Slovak Pinzgau Cattle. Anim. Sci. Biotechnol. 45(1): 211-214. 2012
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Published
2020-03-20
How to Cite
1.
Salazar-Sequea S, De La Rosa O, Marques-Urdaneta A, Vilanova F. L, Vasquez-Marin B. Characterization of Leptin gene polymorphisms in Carora sires: Caracterización de polimorfismos del gen leptina en sementales de la raza Carora. Rev. Cient. FCV-LUZ [Internet]. 2020Mar.20 [cited 2024Nov.22];30(2):82-3. Available from: https://produccioncientificaluz.org/index.php/cientifica/article/view/35550
Section
Animal Production
Copyright (c) 2020 Salazar-Sequea, Saúl A. ; De La Rosa, Oscar; Márques-Urdaneta, Alexis F.; Vilanova-Fernández, Lourdes; Vásquez-Marin, Belkys J.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
