Caracterización de polimorfismos del gen leptina en sementales de la raza Carora
Characterization of Leptin gene polymorphisms in Carora sires
Palabras clave:
Alelo, bovino, genotipo, leptina, polimorfismo
Resumen
El objetivo del presente trabajo fue caracterizar los polimorfismos nucleótidicos simples (SNP) LepClaI (rs29004487), LepKpn2I(rs29004488), LepHphI (rs29004508) y LepSau3AI (rs29004501) del gen Leptina, en toros reproductores de la raza Carora. Se colectaron muestras de sangre y semen de 43 toros nacidos entre 2002 y 2013 para la extracción de ADN; éste se obtuvo a partir de metodologías de precipitación salina. Las genotipificaciones se realizaron a través del análisis de la longitud de los fragmentos de restricción de un producto amplificado (PCR-RFLP) y mediante un sistema de amplificación con cuatro cebadores, refractario a mutaciones (TETRA PRIMER PCR). Los productos de amplificación y digestión fueron verificados en electroforesis horizontal en geles de agarosa. Se calcularon las frecuencias alélicas y genotípicas, la heterocigosidad observada (Ho), y se probó el equilibro de Hardy – Weinberg. Las frecuencias alélicas y genotípicas fueron respectivamente 0,98(A), 0,02(T) y 0,95(AA), 0,05(AT) para el polimorfismo rs29004487; 0,59(C), 0,41(T) y 0,14(CC), 0,53(CT), 0,33(TT) para rs29004488; 0,8(C), 0,2(T) y 0,67(CC), 0,26(CT), 0,07(TT) para rs29004501; 0,94(C), 0,06(T) y 0,88(CC), 0,12(CT) para rs29004508. No se observaron desviaciones del equilibrio de Hardy-Weinberg y se evidencia la existencia de desequilibrio de ligamiento entre la mayoría de los SNP estudiados. Las frecuencias alélicas y genotípicas de los polimorfismos evaluados en la población de toros de raza Carora, son similares a las obtenidas en otras razas. Esta primera aproximación en la caracterización del gen Leptina en ganado Carora, permitirá considerar el potencial genético de la raza para la producción de leche o de carne.
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JI, S.; WILLIS, G.M.; SCOTT, R.R.; SPURLOCK, M.E. Partial cloning and expression of the bovine leptin gene. Anim. Biotechnol.. 9(1): 1-14. 1998
KASPRZAK FILIPEK, K.; SAWICKA ZUGAJ, W.; LITWI´NCZUK, Z.; CHABUZ, W.; SVEISTIENÉ, R.; BULLA, J. Assessement of the genetic structure of central european cattle breeds based on functional gene polymorphism. Global Ecol. Conserv. e00525. 2019
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KONFORTOV, B.A.; LICENCE, V.E.; MILLER, J.R. Re-sequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon. Mammal. Gen.. 10(12): 1142-1145. 1999
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KULIG, H. Association between leptin combined genotypes and milk performance traits of Polish Black and white cows. Arch. Tierz. 48(6): 547-554. 2005
LAGONIGRO, R.; WIENER, P.; PILLA, F.; WOOLLIAMS, J.A.; WILLIAMS, J.L. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Anim. Genet.. 34(5): 371-374. 2003
LEVENE, H. On a matching problem in genetics. Ann. Math. Statist. 20(1): 91-94. 1949
LIEFERS, S.C.; TE PAS, M.F.; VEERKAMP, R.F.; VAN DER LENDE, T. Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake and fertility in Holstein heifers. J. Dairy Sci. 85(6): 1633-1638. 2002
LIEFERS, S.C.; VEERKAMP, R.F.; TE PAS, M.F.; DELAVAUD, C.; CHILLIARTL, M.; PLATJE, M.; VAN DER LENDE, T. Leptin promoter mutation affects leptin levels and performance traits in dairy cows. Anim. Genet. 36(2): 111-118. 2005
MARSON, E.P.; FERRAZ, J.B.; MEIRELLES, F.V.; BALIEIRO, J.C.; ELER, J.P.; FIGUEIREDO, L.G.; MOURAO, G.B. Genetic characterization of European Zebu composite bovine using RFLP markers. Genet. Molec. Res. 4(3): 496-505. 2005
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Publicado
2020-03-20
Cómo citar
1.
Salazar-Sequea S, De La Rosa O, Marques-Urdaneta A, Vilanova F. L, Vasquez-Marin B. Caracterización de polimorfismos del gen leptina en sementales de la raza Carora: Characterization of Leptin gene polymorphisms in Carora sires. Rev. Cient. FCV-LUZ [Internet]. 20 de marzo de 2020 [citado 20 de abril de 2024];30(2):82-3. Disponible en: https://produccioncientificaluz.org/index.php/cientifica/article/view/35550
Sección
Producción Animal
Derechos de autor 2020 Salazar-Sequea, Saúl A. ; De La Rosa, Oscar; Márques-Urdaneta, Alexis F.; Vilanova-Fernández, Lourdes; Vásquez-Marin, Belkys J.
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
