Análisis histológico del epidídimo y los testículos en la dinámica de la ontogénesis postnatal desde un mes hasta la pubertad de los corderos Ouled Djellal

• Yamina Belkhiri University of Batna 2, Faculty of Natural and Life Sciences, Biology of Organisms Department. Batna, Algeria - University of Batna 2, Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology. Batna, Algeria
• Farida Bouzebda–Afri Souk–Ahras University, Biotechnologies and Health. Institute Agronomic and Veterinary Sciences, Laboratory of Animal Productions. Souk Ahras, Algeria
• Zoubir Bouzebda Souk–Ahras University, Biotechnologies and Health. Institute Agronomic and Veterinary Sciences, Laboratory of Animal Productions. Souk Ahras, Algeria
• Souheyla Benbia University of Batna 2, Faculty of Natural and Life Sciences, Biology of Organisms Department. Batna, Algeria - University of Batna 2, Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology. Batna, Algeria
• Ramzi Lamraoui University of Batna 2, Faculty of Natural and Life Sciences, Biology of Organisms Department. Batna, Algeria - Souk–Ahras University, Biotechnologies and Health. Institute Agronomic and Veterinary Sciences, Laboratory of Animal Productions. Souk Ahras, Algeria

DOI: https://doi.org/10.52973/rcfcv-e35521

Palabras clave: Histomorfometría, corderos Ouled Djellal, pubertad, espermatogénesis, testículo, epidídimo

Resumen

El objetivo del estudio fue analizar la ontogenia del epitelio epididimario y seminífero en el testículo de los corderos Ouled Djellal a lo largo del desarrollo postnatal hasta la pubertad. Se utilizaron un total de 24 corderos Ouled Djellal, con edades comprendidas entre 1 y 8 meses, seleccionando tres corderos en cada mes de edad. Los corderos fueron castrados quirúrgicamente mensualmente, y se realizó histología de rutina junto con histomorfometría en los tejidos epididimarios y testiculares procesados utilizando el software AxioVision Rel 4.6. El análisis estadístico reveló que el peso testicular aumentó a un ritmo acelerado entre los 4 y los 8 meses postnatales, coincidiendo con modificaciones significativas en la histología testicular. Estas modificaciones abarcaron incrementos significativos en la medida de los túbulos seminíferos y un incremento en los niveles de testosterona. Se encontró que el diámetro ductal, el diámetro luminal y la altura del epitelio eran estadísticamente diferentes (P<0.05) entre las regiones caput, corpus y cauda del epidídimo, con los niveles máximos registrados durante la pubertad. A los 1 y 2 meses de edad, había dos tipos de células presentes en el epitelio seminífero: células de soporte y gonocitos. A los 3 meses de edad, se formaron los primeros espermatogonios a partir de gonocitos, coincidiendo con el peso promedio de los testículos alcanzando 4 g. La espermatogénesis se inició cuando los gonocitos sufrieron mitosis, dando lugar a progenitores que se diferenciaron en espermatogonios. A los 4 y 5 meses de edad, se observó inicialmente un lumen y espermatocitos primarios (espermatocitos I) en los túbulos seminíferos. A los 6 meses de edad, espermatocitos secundarios y espermatides redondas formaron una única fila dentro de los túbulos seminíferos. A los 7 y 8 meses de edad, todas las generaciones de células madre germinales se encontraban en los seminiferous tubules. A los 8 meses de edad, se observaron espermatozoides en varios segmentos del epidídimo en la especie Ouled Djellal, dando inicio a la etapa de pubertad.

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