Glicoproteínas asociadas a la preñez en búfala: orígenes, funciones y aplicación clínica para el seguimiento de la preñez
Palabras clave:
glicoproteínas asociadas al embarazo, búfalo, el embarazo, manejo reproductivo
Resumen
La eficiencia reproductiva representa un aspecto esencial de la gestión ganadera ya que de ella dependen la productividad y la rentabilidad de una explotación, especialmente en la producción lechera. La detección temprana de la gestación y la identificación de búfalas no gestantes es fundamental en el manejo de la reproducción porque permite una resincronización temprana y una nueva reproducción de los animales no gestantes, acortando el intervalo parto-concepción. Actualmente se encuentran disponibles diferentes herramientas de diagnóstico de la preñez; entre ellos, la utilización de glicoproteínas asociadas a la preñez (PAGs) la cual contribuye significativamente a la mejora del manejo reproductivo. Las PAGs son secretados por la placenta de varias especies de rumiantes, incluida la búfala, y pueden detectarse en la sangre materna. Además de su utilización como herramientas de diagnóstico para identificar animales preñados o no preñados, las PAGs ayudan a identificar la presencia de embriones viables y predecir la mortalidad futura de los embriones durante la gestación temprana. Este artículo presenta una descripción general de los orígenes, la función, la tendencia de preñez y su uso de los PAG para mejorar el manejo reproductivo en la cría de búfalos.
Descargas
La descarga de datos todavía no está disponible.
Citas
Deka, R.P.; Magnusson, U.; Grace, D.; Randolph, T.F.; Shome, R.; Lindahl, J.F. Estimates of the economic cost caused by five major reproductive problems in dairy. Animals 2021, 11, 3116.
Fricke, P.M. Scanning the future–ultrasonography as a reproductive management tool for dairy cattle. J Dairy Sci 2002, 85, 1918–26.
Fricke, P.M.; Ricci, A.; Giordano, J.O.; Carvalho, P.D. Methods for and implementation of pregnancy diagnosis in dairy cows. Vet. Clin. Food Anim. Pract. 2016, 32, 165–180.
Barile, V.L.; Menchetti, L.; Casano, A, B.; Brecchia, G.; Sousa, N.M.; Zelli, R.; Canali, C.; Beckers, S.F.; Barbato, O. Approaches to Identify Pregnancy Failure in Buffalo Cows. Animals 2021, 1, 481.
Xie, S.C.; Low, B.G.; Nagel, R.J.; Kramer, K.K.; Anthony, R.V., Zoli, A.P.; Beckers, J.F. & Roberts, R.M. Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. PNAS 1991, 88, 10247–10251.
Green, J.A.; Xie, S.; Roberts, R.M. Pepsin-related molecules secreted by trophoblast. Reviews of Reproduction 1998, 3, 62–69.
[Beckers, J.F.; Drion, P.V.; Garbayo, J.M., Perenyi Z.; Zarrouk, A. Sulon, J.; Remy, B.; Szenci, O. Pregnancy-associated glycoproteins in ruminantes: inactive members of the aspartic proteinase family. Acta Veterinaria Hungarica 1999, 47, 461-469.
Roberts, R.M.; Xie, S.; Mathialagan, N. Maternal ricognition of pregnancy. Biology of Reproduction 1996, 54, 294-302.
Xie, S.; Low, B.G.; Nagel, R.J.; Beckers, J.F.; Roberts, R.M. A novel glycoprotein of the aspartic proteinase gene family expressed in bovine placental trophectoderm. Biol Reprod 1994, 51, 1145-1153.
Telugu, B.P.; Palmier, M.O.; Van Doren, S.R.; Green, J.A. An examination of the proteolytic activity for bovine pregnancy-associated glycoproteins 2 and 12. Biological Chemistry 2010, 391, 259–270.
Green, J.A.; Xie, S.; Quan, X.; Bao, B.; Mathialagan, N.; Beckers, J.F.; Roberts, R.M. Pregnancy-associated bovine and ovine glycoproteins exhibitspatially and temporally distinct expression patterns during pregnancy. Biol Reprod 2000, 62, 1624-1631.
Zoli, A.P.; Guilbault, L.A.; Delahaut, P.; Ortiz, W.B.; Beckers, J.F. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biology of Reproduction 1992, 46, 83–92.
Touzard, E.; Reinaud, P.; Dubois, O.; Guyader-Joly, C.; Humblot, P.; Ponsart, C.; Charpigny, G. Specific expression patterns and cell distribution of ancient and modern PAG in bovine placenta during pregnancy. Reproduction 2013, 146, 347–362.
Hughes, A.L.; Green, J.A.; Garbayo, J.M.; Roberts, R.M. Adaptive diversification within a large family of recently duplicated, placentally-expressed genes. Proc. Natl. Acad. Sci. USA 2000, 97, 3319–3323.
Wallace, R.M.; Pohler, K.G.; Smith, M.F.; Green, J.A. Placental PAGs: Gene origins, expression patterns, and use as markers of pregnancy. Reproduction 2015, 149, R115–R126
Xie, S.; Green, J.A.; Bao, B.; Beckers, J.F.; Valdez, K.E.; Hakami, L.; Roberts, R.M. Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine placental tissue. Biol Reprod 1997, 57, 1384–1393.
Garbayo, J.M.; Serrano, B.; Lopez-Gatius, F. Identification of novel pregnancyassociated glycoproteins (PAG) expressed by the perimplantion conceptus of domestic ruminants. Anim Reprod Sci 2008, 103, 120–134
Jerome, A.; Singh, S.K.; Agarwal, S.K.; Mohini, S.; Raut A. Characterization and In Silico analysis of pregnancy-associated glycoprotein-1 gene of buffalo (Bubalus bubalis). Genet Res Int 2011, 436138.
Telugu, B.P.; Green, J.A. Characterization of the peptidase activity of recombinant porcine pregnancy-associated glycoprotein-2. Journal of Biochemistry 2008, 144, 725–732.
Moussad, E.E.; Rageh, M.A.; Wilson, AK.; Geisert, R.D.; Brigstock, D.R. Temporal and spatial expression of connective tissue growth factor (CCN2; CTGF) and transforming growth factor beta type 1 (TGF-b1) at the utero-placental interface during early pregnancy in the pig. Journal of Clinical Pathology: Molecular Pathology 2002, 55, 186-192
Wooding, F.B.; Roberts, R.M.; Green, J.A. Light and electron microscope immunocytochemical studies of the distribution of pregnancy-associated glycoproteins (PAGs) throughout pregnancy in the cow: possible functional implications. Placenta, 2005, 26, 807–827.
Dosogne, H.; Burvenich, C.; Freeman, A.E.; Kehrli Jr, M.E.; Detilleux, J.C., Sulon, J., Beckers, J.F.; Hoeben, D. Pregnancy-associated glycoprotein and decreased polymorphonuclear leukocyte function in early post-prtum dairy cows. Vet Immunology and immunopathology 1999, 67, 47-54.
Austin, K.J.; King, C.P.; Vierk, J.E.; Sasser, R.G.; Hansen, T.R. Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium. Endocrinology 1999, 140, 542–545.
Del Vecchio, R.P.; Sutherland, W.D.; Sasser, R.G. Bovine luteal cell production in vitro of prostaglandin E2, oxytocin and progesterone in response to pregnancy-specific protein B and prostaglandin F2a. Journal of Reproduction and Fertility 1996, 107, 131–136.
Weems, Y.S.; Lammoglia, M.A.; Vera-Avila, H.R.; Randel, R.D.; King, C.; Sasser, R.G.; Weems, C.W. Effect of luteinizing hormone (LH), PGE2, 8-Epi- PGE1, 8-EPI- PGE2, trichosanthin, and pregnancy specific protein B on secretion of progesterone in vitro by corpora lutea (CL) from nonpregnant and pregnant cows. Prostaglandins & Other Lipid Mediators 1998, 55, 27–42.
Kornmatitsuk, B.; Veronesi, M.C.; Madej, A.; Dahl, E.; Ropstad, E.; Beckers, J.F.; Forsberg, M.; Gustalsson, H.; Kindhal, H. Hormonal measurements in late pregnancy and parturition in dairy cows-possible tools to monitor fetal well being. Anim Reprod Sci 2002, 72, 153-164.
Giordano, J.O.; Guenther, J.N.; Lopes, G. Jr; Fricke, P.M. Changes in serum pregnancy-associated glycoprotein, pregnancy-specific protein B, and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows. Journal of Dairy Science 2012, 95, 683–697.
Karen, A.; De Sousa, N.M.; Beckers, J.F.; Bajcsy, A.C.; Tibold, J.; Madl, I.; Szenci, O. Comparison of a commercial bovine pregnancy-associated glycoprotein ELISA test amd pregnancy-associated glycoprotein radioimmunoassay test for early pregnancy diagnosis in dairy cattle. Anim Reprod Sci 2015, 159, 31-37.
Haugejorden, G.; Waage, S.; Dahl, E.; Karlberg, K.; Beckers, J.F.; Ropstad, E. Pregnancy associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring. Theriogenology 2006, 66, 1976-1984.
Franco, G.A.; Peres, R.F.G.; Martins, C.F.G.; Reese, S.T.; Jose Luiz Moraes Vasconcelos, J.L.M.; Pohler, K.J. Sire contribution to pregnancy loss and pregnancy-associated glycoprotein production in Nelore cows. J. Anim. Sci. 2018, 96, 632–640.
Reese, S.T., Geary, T.W.; Franco, G.A.; Moares, J.G.N.; Spencer, T.E.; Pohler, K.G. Pregnancy associated glycoproteins (PAGs) and pregnancy loss in high vs sub fertility heifers. Theriogenology 2019, 135, 7-12.
Pohler, K.G.; Reese, S.T.; Franco, G.A.; Oliveira, R.V.; Paiva, R.; Fernandez, L.; de Melo, G.; Vasconcelos, J.L.M.; Cooke, R.; Poole, R.K. New approaches to diagnose and target reproductive failure in cattle. Anim. Reprod. 2020, 17.
Barile, V.L. Improving reproductive efficiency in female buffaloes. (Review). Liv. Prod. Science, 2005, 92, 183-194.
Barile, V.; Terzano, G.; Pacelli, C.; Todini, L.; Malfatti, A.; Barbato, O. LH peak and ovulation after two different estrus synchronization treatments in buffalo cows in the daylight-lengthening period. Theriogenology 2015, 84,
286–293.
Baruselli, P.; Visintin, J.; Barnabe, V.; Barnabe, R.; Amaral, R.; Souza, A. Early pregnancy ultrasonography and embryonic mortality occurence in buffalo. In Proceedings of the V World Buffalo Congress,1997. Caserta, Italy, 13–18 October 1997; pp. 13–16.
Campanile, G.; Neglia, G.; D’Occhio, M.J. Embryonic and fetal mortality in river buffalo (Bubalus bubalis). The riogenology, 2016, 86, 207-213.
Karen, A.; Darwish, S.; Ramoun, A.; Tawfeek, K.; Van Hanh, N.; De Sousa, N.; Sulon, J.; Szenci, O.; Beckers, J.-F. Accuracy of ultrasonography and pregnancy-associated glycoprotein test for pregnancy diagnosis in buffaloes. Theriogenology 2007, 68, 1150–1155.
El-Battawy, K.A.; Sousa, N.M.; Szenci, O.; Beckers, J.F. Pregnancy-associated glycoprotein profile during the first trimester of pregnancy in Egyptian buffalo cows. Reprod Domest Anim Zuchthyg 2009, 44, 161–166.
Barbato, O.; Sousa, N.M.; Klisch, K.; Clerget, E.; Debenedetti, A.; Barile, V.L.; Malfatti, A.; Beckers, J.F. Isolation of new pregnancyassociated glycoproteins from water buffalo (Bubalus bubalis) placenta by Vicia villosa affinity chromatography. Res Vet Sci 2008, 85, 457–466.
Barbato, O.; Melo de Sousa, N.; Barile, V.L.; Canali, C.; Beckers, J.-F. Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females. BMC Vet Res 2013, 9, 89.
Barbato, O.; Menchetti, L.; Sousa, N.M.; Malfatti, A.; Brecchia, G.; Canali, C.; Beckers, J.F.; Barile, V.L. Pregnancy-associated glycoproteins (PAGs) concentrations in water buffaloes (Bubalus bubalis) during gestation and the postpartum period. Theriogenology 2017, 97, 73–77.
Barbato, O.; Menchetti, L.; Sousa, N.M.; Brecchia, G.; Malfatti, A.; Canali, C.; Beckers J.F.; Barile, V.L. Correlation of two radioimmunoassay systems for measuring plasma pregnancy-associated glycoprotein concentrations during early pregnancy and postpartum periods in water buffalo. Reprod Domest Anim 2018, 53, 1483-149.
Karen. A.; Darwish, S.; Ramoun, A.; Tawfeek, K.; Van Hanh, N.; Sousa, N.M.; Sulon. J.; Szenci, O.; Beckers, J.F. Accuracy of ultrasonography and pregnancy-associated glycoprotein test for pregnancy diagnosis in buffaloes. Theriogenology 2007, 68, 1150–1155.
Nguyen, V.H.; Barbato, O.; Bui, X.N.; Beckers, J.-F.; de Sousa, N.M. Assessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera. Anim Sci J 2012, 83, 683–689.
Barbato, O.; Barile, V.L. The pregnancy diagnosis in buffalo species: laboratory methods. J Buffalo Sci 2012, 1, 157-162.
Barbato, O.; Chiaradia, E.; Barile, V.L.; Pierri, F.; Sousa, M.N.; Terracina, L.: Canali, C.; Avellini, L.; Investigation into omocysteine, vitamin E and malondialdeyde as indicators of successful artificial insemination in synchronized buffalo cows (Bubalus bubalis). Research in Veterinary Science 2016, 104, 100-105.
Pacelli, C.; Barile, V.L.; Sabia, E.; Casano, A.B.; Braghieri, A.; Martina, V.; Barbato, O. Use of GnRH Treatment Based on Pregnancy-Associated Glyco-Proteins (PAGs) Levels as a Strategy for the Maintenance of Pregnancy in Buffalo Cows: A Field Study. Animals 2022, 12, 2822.
Mann, G.E; Lamming, G.E.; Fray, M.D. Plasma oestradiol and progesterone during early pregnancy in the cow and the effects of treatment with buserelin. Anim. Reprod. Sci. 1995, 37, 121–131.
Thatcher, W.W.; Meyer, M.D.; Danet-Desnoyers, G. Maternal Recognition of Pregnancy. J. Reprod. Fertil.-Suppl. 1995, 49, 15–28.
Saugandhika, S.; Sharma, V.; Malik, H.; Saini, S.; Bag, S.; Kumar, S.; Singh, N.K.; Mohanty, A.K.; Malakar, D. Expression and Purification of Buffalo Interferon-Tau and Efficacy of Recombinant Buffalo Interferon-Tau for in Vitro Embryo Development. Cytokine 2015, 75, 186–196.
Rocha CC, Silveira JC, Forde N, Binelli M, Pugliesi G. Conceptus-modulated innate immune function during early pregnancy in ruminants : a review. Anim Reprod. 2021,18(1) :e20200048.
Casano, A.B; Barile, V.L.; Menchetti, L.; Guelfi, G.; Brecchia, G.; Agradi, S.; De Matteis, G.; Scatà, M.C.; Grandoni, F.; Barbato, O. Interferon Tau (IFNt) and Interferon-Stimulated Genes (ISGs) Expression in Peripheral Blood Leukocytes and Correlation with Circulating Pregnancy-Associated Glycoproteins (PAGs) during Peri-Implantation and Early Pregnancy in Buffalo Cows. Animals, 2022, 12, 3068.
Casano A.B. Menchetti L., Trabalza-Marinucci M., Riva F, De Matteis G., Brecchia G., Inglesi A, Rossi E., Signorelli F., Barile V.L., Barbato O. Gene expression of pregnancy-associated glycoproteins-1 (PAG-1), interferontau (IFNt) and interferon stimulated genes (ISGs) as diagnostic and prognostic markers of maternal-fetal cellular interaction in buffalo cows. Theriogenology, 2023, 209, 89-97.
Barbato O, Guelfi G, Barile VL, Menchetti L, Tortiello C, Canali C, Brecchia G, Traina G, Beckers JF, Melo de Sousa N. 2017. Using real-time PCR to identify pregnancy-associated glycoprotein 2 (PAG-2) in water buffalo (bubalus bubalis) blood in early pregnancy. Theriogenology 2017, 89, 106-113.
Barbato, O.; Guelfi, G.; Menchetti, L.; Brecchia, G.; Sousa, M.N.; Canali, C.; Grandoni, F.; Scatà, M.C.; De Matteis, G.; Casano, A.B.; Beckers, J.F.; Barile, V.L. Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period. Veterinary Sciences 2019, 6, 8.
Fricke, P.M. Scanning the future–ultrasonography as a reproductive management tool for dairy cattle. J Dairy Sci 2002, 85, 1918–26.
Fricke, P.M.; Ricci, A.; Giordano, J.O.; Carvalho, P.D. Methods for and implementation of pregnancy diagnosis in dairy cows. Vet. Clin. Food Anim. Pract. 2016, 32, 165–180.
Barile, V.L.; Menchetti, L.; Casano, A, B.; Brecchia, G.; Sousa, N.M.; Zelli, R.; Canali, C.; Beckers, S.F.; Barbato, O. Approaches to Identify Pregnancy Failure in Buffalo Cows. Animals 2021, 1, 481.
Xie, S.C.; Low, B.G.; Nagel, R.J.; Kramer, K.K.; Anthony, R.V., Zoli, A.P.; Beckers, J.F. & Roberts, R.M. Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. PNAS 1991, 88, 10247–10251.
Green, J.A.; Xie, S.; Roberts, R.M. Pepsin-related molecules secreted by trophoblast. Reviews of Reproduction 1998, 3, 62–69.
[Beckers, J.F.; Drion, P.V.; Garbayo, J.M., Perenyi Z.; Zarrouk, A. Sulon, J.; Remy, B.; Szenci, O. Pregnancy-associated glycoproteins in ruminantes: inactive members of the aspartic proteinase family. Acta Veterinaria Hungarica 1999, 47, 461-469.
Roberts, R.M.; Xie, S.; Mathialagan, N. Maternal ricognition of pregnancy. Biology of Reproduction 1996, 54, 294-302.
Xie, S.; Low, B.G.; Nagel, R.J.; Beckers, J.F.; Roberts, R.M. A novel glycoprotein of the aspartic proteinase gene family expressed in bovine placental trophectoderm. Biol Reprod 1994, 51, 1145-1153.
Telugu, B.P.; Palmier, M.O.; Van Doren, S.R.; Green, J.A. An examination of the proteolytic activity for bovine pregnancy-associated glycoproteins 2 and 12. Biological Chemistry 2010, 391, 259–270.
Green, J.A.; Xie, S.; Quan, X.; Bao, B.; Mathialagan, N.; Beckers, J.F.; Roberts, R.M. Pregnancy-associated bovine and ovine glycoproteins exhibitspatially and temporally distinct expression patterns during pregnancy. Biol Reprod 2000, 62, 1624-1631.
Zoli, A.P.; Guilbault, L.A.; Delahaut, P.; Ortiz, W.B.; Beckers, J.F. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biology of Reproduction 1992, 46, 83–92.
Touzard, E.; Reinaud, P.; Dubois, O.; Guyader-Joly, C.; Humblot, P.; Ponsart, C.; Charpigny, G. Specific expression patterns and cell distribution of ancient and modern PAG in bovine placenta during pregnancy. Reproduction 2013, 146, 347–362.
Hughes, A.L.; Green, J.A.; Garbayo, J.M.; Roberts, R.M. Adaptive diversification within a large family of recently duplicated, placentally-expressed genes. Proc. Natl. Acad. Sci. USA 2000, 97, 3319–3323.
Wallace, R.M.; Pohler, K.G.; Smith, M.F.; Green, J.A. Placental PAGs: Gene origins, expression patterns, and use as markers of pregnancy. Reproduction 2015, 149, R115–R126
Xie, S.; Green, J.A.; Bao, B.; Beckers, J.F.; Valdez, K.E.; Hakami, L.; Roberts, R.M. Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine placental tissue. Biol Reprod 1997, 57, 1384–1393.
Garbayo, J.M.; Serrano, B.; Lopez-Gatius, F. Identification of novel pregnancyassociated glycoproteins (PAG) expressed by the perimplantion conceptus of domestic ruminants. Anim Reprod Sci 2008, 103, 120–134
Jerome, A.; Singh, S.K.; Agarwal, S.K.; Mohini, S.; Raut A. Characterization and In Silico analysis of pregnancy-associated glycoprotein-1 gene of buffalo (Bubalus bubalis). Genet Res Int 2011, 436138.
Telugu, B.P.; Green, J.A. Characterization of the peptidase activity of recombinant porcine pregnancy-associated glycoprotein-2. Journal of Biochemistry 2008, 144, 725–732.
Moussad, E.E.; Rageh, M.A.; Wilson, AK.; Geisert, R.D.; Brigstock, D.R. Temporal and spatial expression of connective tissue growth factor (CCN2; CTGF) and transforming growth factor beta type 1 (TGF-b1) at the utero-placental interface during early pregnancy in the pig. Journal of Clinical Pathology: Molecular Pathology 2002, 55, 186-192
Wooding, F.B.; Roberts, R.M.; Green, J.A. Light and electron microscope immunocytochemical studies of the distribution of pregnancy-associated glycoproteins (PAGs) throughout pregnancy in the cow: possible functional implications. Placenta, 2005, 26, 807–827.
Dosogne, H.; Burvenich, C.; Freeman, A.E.; Kehrli Jr, M.E.; Detilleux, J.C., Sulon, J., Beckers, J.F.; Hoeben, D. Pregnancy-associated glycoprotein and decreased polymorphonuclear leukocyte function in early post-prtum dairy cows. Vet Immunology and immunopathology 1999, 67, 47-54.
Austin, K.J.; King, C.P.; Vierk, J.E.; Sasser, R.G.; Hansen, T.R. Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium. Endocrinology 1999, 140, 542–545.
Del Vecchio, R.P.; Sutherland, W.D.; Sasser, R.G. Bovine luteal cell production in vitro of prostaglandin E2, oxytocin and progesterone in response to pregnancy-specific protein B and prostaglandin F2a. Journal of Reproduction and Fertility 1996, 107, 131–136.
Weems, Y.S.; Lammoglia, M.A.; Vera-Avila, H.R.; Randel, R.D.; King, C.; Sasser, R.G.; Weems, C.W. Effect of luteinizing hormone (LH), PGE2, 8-Epi- PGE1, 8-EPI- PGE2, trichosanthin, and pregnancy specific protein B on secretion of progesterone in vitro by corpora lutea (CL) from nonpregnant and pregnant cows. Prostaglandins & Other Lipid Mediators 1998, 55, 27–42.
Kornmatitsuk, B.; Veronesi, M.C.; Madej, A.; Dahl, E.; Ropstad, E.; Beckers, J.F.; Forsberg, M.; Gustalsson, H.; Kindhal, H. Hormonal measurements in late pregnancy and parturition in dairy cows-possible tools to monitor fetal well being. Anim Reprod Sci 2002, 72, 153-164.
Giordano, J.O.; Guenther, J.N.; Lopes, G. Jr; Fricke, P.M. Changes in serum pregnancy-associated glycoprotein, pregnancy-specific protein B, and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows. Journal of Dairy Science 2012, 95, 683–697.
Karen, A.; De Sousa, N.M.; Beckers, J.F.; Bajcsy, A.C.; Tibold, J.; Madl, I.; Szenci, O. Comparison of a commercial bovine pregnancy-associated glycoprotein ELISA test amd pregnancy-associated glycoprotein radioimmunoassay test for early pregnancy diagnosis in dairy cattle. Anim Reprod Sci 2015, 159, 31-37.
Haugejorden, G.; Waage, S.; Dahl, E.; Karlberg, K.; Beckers, J.F.; Ropstad, E. Pregnancy associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring. Theriogenology 2006, 66, 1976-1984.
Franco, G.A.; Peres, R.F.G.; Martins, C.F.G.; Reese, S.T.; Jose Luiz Moraes Vasconcelos, J.L.M.; Pohler, K.J. Sire contribution to pregnancy loss and pregnancy-associated glycoprotein production in Nelore cows. J. Anim. Sci. 2018, 96, 632–640.
Reese, S.T., Geary, T.W.; Franco, G.A.; Moares, J.G.N.; Spencer, T.E.; Pohler, K.G. Pregnancy associated glycoproteins (PAGs) and pregnancy loss in high vs sub fertility heifers. Theriogenology 2019, 135, 7-12.
Pohler, K.G.; Reese, S.T.; Franco, G.A.; Oliveira, R.V.; Paiva, R.; Fernandez, L.; de Melo, G.; Vasconcelos, J.L.M.; Cooke, R.; Poole, R.K. New approaches to diagnose and target reproductive failure in cattle. Anim. Reprod. 2020, 17.
Barile, V.L. Improving reproductive efficiency in female buffaloes. (Review). Liv. Prod. Science, 2005, 92, 183-194.
Barile, V.; Terzano, G.; Pacelli, C.; Todini, L.; Malfatti, A.; Barbato, O. LH peak and ovulation after two different estrus synchronization treatments in buffalo cows in the daylight-lengthening period. Theriogenology 2015, 84,
286–293.
Baruselli, P.; Visintin, J.; Barnabe, V.; Barnabe, R.; Amaral, R.; Souza, A. Early pregnancy ultrasonography and embryonic mortality occurence in buffalo. In Proceedings of the V World Buffalo Congress,1997. Caserta, Italy, 13–18 October 1997; pp. 13–16.
Campanile, G.; Neglia, G.; D’Occhio, M.J. Embryonic and fetal mortality in river buffalo (Bubalus bubalis). The riogenology, 2016, 86, 207-213.
Karen, A.; Darwish, S.; Ramoun, A.; Tawfeek, K.; Van Hanh, N.; De Sousa, N.; Sulon, J.; Szenci, O.; Beckers, J.-F. Accuracy of ultrasonography and pregnancy-associated glycoprotein test for pregnancy diagnosis in buffaloes. Theriogenology 2007, 68, 1150–1155.
El-Battawy, K.A.; Sousa, N.M.; Szenci, O.; Beckers, J.F. Pregnancy-associated glycoprotein profile during the first trimester of pregnancy in Egyptian buffalo cows. Reprod Domest Anim Zuchthyg 2009, 44, 161–166.
Barbato, O.; Sousa, N.M.; Klisch, K.; Clerget, E.; Debenedetti, A.; Barile, V.L.; Malfatti, A.; Beckers, J.F. Isolation of new pregnancyassociated glycoproteins from water buffalo (Bubalus bubalis) placenta by Vicia villosa affinity chromatography. Res Vet Sci 2008, 85, 457–466.
Barbato, O.; Melo de Sousa, N.; Barile, V.L.; Canali, C.; Beckers, J.-F. Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females. BMC Vet Res 2013, 9, 89.
Barbato, O.; Menchetti, L.; Sousa, N.M.; Malfatti, A.; Brecchia, G.; Canali, C.; Beckers, J.F.; Barile, V.L. Pregnancy-associated glycoproteins (PAGs) concentrations in water buffaloes (Bubalus bubalis) during gestation and the postpartum period. Theriogenology 2017, 97, 73–77.
Barbato, O.; Menchetti, L.; Sousa, N.M.; Brecchia, G.; Malfatti, A.; Canali, C.; Beckers J.F.; Barile, V.L. Correlation of two radioimmunoassay systems for measuring plasma pregnancy-associated glycoprotein concentrations during early pregnancy and postpartum periods in water buffalo. Reprod Domest Anim 2018, 53, 1483-149.
Karen. A.; Darwish, S.; Ramoun, A.; Tawfeek, K.; Van Hanh, N.; Sousa, N.M.; Sulon. J.; Szenci, O.; Beckers, J.F. Accuracy of ultrasonography and pregnancy-associated glycoprotein test for pregnancy diagnosis in buffaloes. Theriogenology 2007, 68, 1150–1155.
Nguyen, V.H.; Barbato, O.; Bui, X.N.; Beckers, J.-F.; de Sousa, N.M. Assessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera. Anim Sci J 2012, 83, 683–689.
Barbato, O.; Barile, V.L. The pregnancy diagnosis in buffalo species: laboratory methods. J Buffalo Sci 2012, 1, 157-162.
Barbato, O.; Chiaradia, E.; Barile, V.L.; Pierri, F.; Sousa, M.N.; Terracina, L.: Canali, C.; Avellini, L.; Investigation into omocysteine, vitamin E and malondialdeyde as indicators of successful artificial insemination in synchronized buffalo cows (Bubalus bubalis). Research in Veterinary Science 2016, 104, 100-105.
Pacelli, C.; Barile, V.L.; Sabia, E.; Casano, A.B.; Braghieri, A.; Martina, V.; Barbato, O. Use of GnRH Treatment Based on Pregnancy-Associated Glyco-Proteins (PAGs) Levels as a Strategy for the Maintenance of Pregnancy in Buffalo Cows: A Field Study. Animals 2022, 12, 2822.
Mann, G.E; Lamming, G.E.; Fray, M.D. Plasma oestradiol and progesterone during early pregnancy in the cow and the effects of treatment with buserelin. Anim. Reprod. Sci. 1995, 37, 121–131.
Thatcher, W.W.; Meyer, M.D.; Danet-Desnoyers, G. Maternal Recognition of Pregnancy. J. Reprod. Fertil.-Suppl. 1995, 49, 15–28.
Saugandhika, S.; Sharma, V.; Malik, H.; Saini, S.; Bag, S.; Kumar, S.; Singh, N.K.; Mohanty, A.K.; Malakar, D. Expression and Purification of Buffalo Interferon-Tau and Efficacy of Recombinant Buffalo Interferon-Tau for in Vitro Embryo Development. Cytokine 2015, 75, 186–196.
Rocha CC, Silveira JC, Forde N, Binelli M, Pugliesi G. Conceptus-modulated innate immune function during early pregnancy in ruminants : a review. Anim Reprod. 2021,18(1) :e20200048.
Casano, A.B; Barile, V.L.; Menchetti, L.; Guelfi, G.; Brecchia, G.; Agradi, S.; De Matteis, G.; Scatà, M.C.; Grandoni, F.; Barbato, O. Interferon Tau (IFNt) and Interferon-Stimulated Genes (ISGs) Expression in Peripheral Blood Leukocytes and Correlation with Circulating Pregnancy-Associated Glycoproteins (PAGs) during Peri-Implantation and Early Pregnancy in Buffalo Cows. Animals, 2022, 12, 3068.
Casano A.B. Menchetti L., Trabalza-Marinucci M., Riva F, De Matteis G., Brecchia G., Inglesi A, Rossi E., Signorelli F., Barile V.L., Barbato O. Gene expression of pregnancy-associated glycoproteins-1 (PAG-1), interferontau (IFNt) and interferon stimulated genes (ISGs) as diagnostic and prognostic markers of maternal-fetal cellular interaction in buffalo cows. Theriogenology, 2023, 209, 89-97.
Barbato O, Guelfi G, Barile VL, Menchetti L, Tortiello C, Canali C, Brecchia G, Traina G, Beckers JF, Melo de Sousa N. 2017. Using real-time PCR to identify pregnancy-associated glycoprotein 2 (PAG-2) in water buffalo (bubalus bubalis) blood in early pregnancy. Theriogenology 2017, 89, 106-113.
Barbato, O.; Guelfi, G.; Menchetti, L.; Brecchia, G.; Sousa, M.N.; Canali, C.; Grandoni, F.; Scatà, M.C.; De Matteis, G.; Casano, A.B.; Beckers, J.F.; Barile, V.L. Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period. Veterinary Sciences 2019, 6, 8.
Publicado
2023-11-21
Cómo citar
1.
Barbato O, Barile VL. Glicoproteínas asociadas a la preñez en búfala: orígenes, funciones y aplicación clínica para el seguimiento de la preñez. Rev. Cient. FCV-LUZ [Internet]. 21 de noviembre de 2023 [citado 12 de marzo de 2025];33(Suplemento):75-1. Disponible en: https://produccioncientificaluz.org/index.php/cientifica/article/view/43291
Sección
Conferencias
