Identificación de Vagococcus salmoninarum en trucha arcoíris (Oncorhynchus mykiss Walbaum, 1792) mediante espectrometría de masas MALDI–TOF basada en proteómica

• İfakat Tülay Çağatay Akdeniz University, Faculty of Fisheries, Department of Basic Sciences, Molecular Microbiology Laboratory. Antalya, Türkiye

DOI: https://doi.org/10.52973/rcfcv-e34462

Palabras clave: Vagococcus salmoninarum, Vagococcosis, BBL Crystal™ GP, PCR, MALDI–TOF MS

Resumen

Vagococcus salmoninarum es un patógeno que causa vagococosis en reproductores de trucha arcoíris (Oncorhynchus mykiss, Walbaum 1792) y peces grandes. En este estudio, se probaron juntos los métodos de diagnóstico convencionales, incluidos el fenotípico, el genómico y el proteómico de alto rendimiento MALDI–TOF MS, para lograr una identificación precisa y rápida de V. salmoninarum. Doce aislados obtenidos de órganos internos ampliamente infectados de un total de cincuenta peces fueron confirmados fenotípicamente como V. salmoninarum mediante características bioquímicas y enzimáticas con el sistema de identificación BBL Crystal™ GP. Sin embargo, la presencia de amplificación de PCR positiva del gen 16S rDNA se detectó solo en el 83 % de estos aislamientos y las mismas muestras se identificaron como V. salmoninarum mediante el método MALDI–TOF MS con un valor de puntuación de masa alto (m/z) entre 2.00 y 3.00. Con base en los datos comparativos obtenidos en este estudio, concluimos que el método MALDI–TOF MS es el método más prometedor y recomendado para la identificación definitiva de V. salmoninarum.

Citas

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