Evaluación de la aplicación y efectos del uso intratesticular de vaselina y mezcla de ácido acetilsalicílico + vaselina al 30 % para fines de castración química en ratas machos

• Cafer Tayer İşler Mustafa Kemal Üniversity, Faculty of Veterinary Medicine, Department of Surgery. Türkiye
• Onur Bakır Phoenix petcare veterinary clinic. Ankara, Türkiye
• Ahmet Uyar Mustafa Kemal Üniversity, Faculty of Veterinary Medicine, Department of Pathology. Türkiye
• Mehmet Güvenç Mustafa Kemal Üniversity, Faculty of Veterinary Medicine, Department of Physiology, Türkiye
• Oğuz Kaan Yalçın Mustafa Kemal Üniversity, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination. Türkiye

DOI: https://doi.org/10.52973/rcfcv-e34424

Palabras clave: Castración química, vaselina, ácido acetilsalicílico, parámetros oxidativos, rata

Resumen

El objetivo de este estudio fue evaluar la aplicación y los efectos del uso intratesticular de una mezcla que contiene vaselina y ácido acetilsalicílico al 30% para la castración química en ratas macho. Veintiocho ratas Wistar albinas macho se dividieron en cuatro grupos: control, simulado, vaselina y mezcla de vaselina + ácido salicílico al 30%. El grupo de control (K) no recibió ninguna inyección, al grupo simulado (S) se le inyectó 1 mL de solución salina, al grupo de vaselina (V) se le inyectó 1 mL de vaselina, y al grupo de vaselina + ácido acetilsalicílico (V+ASA) se le inyectó 1 mL de una mezcla que contenía ácido acetilsalicílico al 30% y vaselina. Se determinó una diferencia estadística altamente significativa (P=0.000) entre los grupos de vaselina (V) y vaselina + ácido acetilsalicílico (V+ASA). El valor de malondialdehído (MDA) del grupo de vaselina fue encontrado más alto (3.197 ± 0.08) con una diferencia estadística altamente significativa (P=0.000) en comparación con los otros grupos. En términos del valor de glutatión (GSH), se observó una diferencia estadística significativa (P<0.05) en el grupo V+ASA en comparación con los grupos V y K. Al comparar los niveles de glutatión peroxidasa (GSH–Px), se encontró una diferencia altamente significativa (P=0.013) entre los grupos de vaselina (V) y control (K). Se encontró una diferencia estadística significativa (P=0.046) entre los grupos de vaselina (V) y vaselina + ácido acetilsalicílico (V+ASA) en términos de actividades de catalasa (CAT). En términos de motilidad en el examen espermatológico, se determinó que el grupo de vaselina (V) tenía la tasa más baja con 11,250 ± 3.14, mostrando una diferencia estadística altamente significativa (P=0.001) en comparación con los otros tres grupos. El grupo con la mayor concentración de esperma fue el grupo K (82,000 ± 6.60), mientras que la concentración más baja se observó en el grupo V (27,600 ± 3.54). La proporción de esperma muerto/viable en el semen se encontró más baja (7,200 ± 2.03) en el grupo K y más alta (61,250 ± 13.16) en el grupo V. La tasa más alta y la diferencia estadística en los valores de un cromosoma se determinaron en el grupo de vaselina (V). Histopatológicamente, la morfología de los túbulos testiculares se vio afectada en los grupos de vaselina (V) y vaselina + ácido acetilsalicílico (V+ASA), observada como degenerada y necrótica. Se observaron túbulos degenerados, desprovistos de células epiteliales germinativas y compuestos solo por la membrana basal. En conclusión, el proceso de castración se realizó de manera irreversible en los grupos de vaselina (V) y vaselina + ácido acetilsalicílico (V+ASA).

Citas

Rafatmah D, Mogheiseh A, Eshghi D. Chemical sterilization with intratesticular administration of zinc gluconate in adult dogs: a preliminary report. Basic. Clin. Androl. [Internet]. 2019; 29(12):1–9. doi: https://doi.org/gt564p

Vendramini TH, Amaral AR, Pedrinelli V, Zafalon RV, Rodrigues RBA, Brunetto MA. Neutering in dogs and cats: current scientific evidence and importance of adequate nutritional management. Nutr. Res. Rev. [Internet]. 2020; 33(1):134–144. doi: https://doi.org/gnb24h

Jana K, Samanta PK. Clinical Evaluation of non–surgical sterilization of male cats with single intra–testicular injection of calcium chloride. BMC Vet. Res. [Internet]. 2011; 7(39):1–15. doi: https://doi.org/fdbmnx

Hamilton KH, Henderson ER, Toscano M, Chanoit GP. Comparison of postoperative complications in healthy dogs undergoing open and closed orchidectomy. J.Small Anim. Pract. [Internet]. 2014; 55(10):521–526. doi: https://doi.org/gt56x5

El–Wahed REA, Korittum AS, Abu–Ahmed HM, Sahwan AAS. Evaluation of pinhole castration technique compared with traditional method for castration in dogs. Alex. J. Vet. Sci. [Internet]. 2014; 42(1):90–98. doi: https://doi.org/gt564q

Garde E, Pérez GE, Vanderstichel R, Dalla Villa PF, Serpell JA. Effects of surgical and chemical sterilization on the behavior of free–roaming male dogs in Puerto Natales, Chile. Prev. Vet. Med. [Internet]. 2016; 123:106–120. doi: https://doi.org/f7979t

Tez G, Kanca H, Alemdar H. Köpeklerde kontrasepsiyon I: Cerrahi sterilizasyonun risk ve yararlarının değerlendirilmesi [Contraception in dogs I: Evaluation of surgical sterilization risk and benefits]. Vet. Hekim Fak. Derg. [Internet]. 2019; 90(1):55–65. Turkish. doi: https://doi.org/gt56x6

Başa A, Canpolat İ. Chemical Sterilization in Domestic Animals. Res Agri. Vet. Sci. [Internet]. 2019 [cited 12 Dec. 2023]; 3(1):5–9. Available in: https://goo.su/nvT9H

Baran A, Özdeş ÖB, Sandal Aİ. Erkek Kedi ve Köpeklerde Üremenin Önlenmesi [Prevention of Fertility in Male Cat and Dogs]. Türkiye Klinikleri J. Vet. Sci. Obstet. Gynecol–Special Topics [Internet]. 2016 [cited 12 Feb 2024]; 2(2):9–18. Turkish. Available in: https://goo.su/nShwG2Y

Fesseha H. Non–Surgical Sterilization methods in male animals: A Review. Vet. Med. Open J. [Internet]. 2019; 4(2):49–56. doi: https://doi.org/gt56x7

Dixit VP. Chemical sterilization of male dogs (Canis familiaris) after single intratesticular administration of methalibure (ICI–33828), dexamethasone, metoprion (SU–4885, Ciba), niridazole (33644–Ba, Ciba) alpha–chlorohydrin (U–5897) and danazol. Indian J. Exp. Biol. 1979; 17(9):937–940. PMID: 544471.

Anand LN, Vijayan E. Studies on the Effect of Intratesticular Administration of Opioid Peptides, Naloxone or N–Acetyl – Endorphin Antiserum on some Testicular Parameters in Rats. Indian J. Physiol Pharmacol. [Internet]. 1998 [cited 22 Feb. 2024]; 42(1):107–112. Available in: https://goo.su/55bJYvu

Günay C, Sağlıyan A, Yaman İ, Sönmez M, Türk G. Erkek köpeklerde intratestiküler Gliserol ve Etanol Uygulamalarının Kısırlaştırma üzerine etkilerinin karşılaştırılması [Comporation of the Effects of Intratesticular Glycerol and Ethanol Injections on Sterilization of Male Dogs]. Türk Vet. Cer. Derg. [Internet]. 2004 [cited 12 Feb. 2024]; 10(1–2):55–60. Turkish. Available in: https://goo.su/4JvUod

Theubet G, Thun R, Hilbe M, Janett F. Wirkung einer Impfung gegen GnRH (Bopriva®) beim männlichen pubertären Kalb [Effect of vaccination against GnRH (Bopriva®) in the male pubertal calf]. Schweiz. Arch. Tierheilkd [Internet]. 2010; 152(10):459–469. German. doi: https://doi.org/cxd638

Canpolat İ, Gür S, Günay C, Bulut S, Eröksüz H,. An evaluation of the outcome of bull castration by intra testicular injection of ethanol and calcium. Revue Méd. Vét. [Internet]. 2006 [cited 22 Jan. 2024]; 157(8–9):420–425. Available in: https://goo.su/za8E

Oliveira ECS, Moura MR, Silva Jr. VA, Peixoto CA, Saraiva KLA, de Sá MJC, Douglas RH, Marques Jr. AP. Intratesticular injection of a zinc–based solution as a contraceptive for dogs. Theriogenology [Internet]. 2007; 68(2):137–145. doi: https://doi.org/bktvm2

Leoci R, Aiudi G, Silvestre F, Lissner EA, Marino F, Lacalandra GM. A dose finding, long–term study on the use of calcium chloride in saline solution as a method of nonsurgical sterilization in dogs: evaluation of the most effective concentration with the lowest risk. Acta Vet. Scand. [Internet]. 2014; 56(63):1–8. doi: https://doi.org/f6kvbj

Sen CC, Yumuşak N, Faundez R, Temamogullari F, Taşkin A. Evaluation of intra–testicular injections of calcium chloride and 4–vinylcyclohexene 1, 2 monoepoxide for chemical sterilization in guinea pigs. Pol. J. Vet. Sci. [Internet]. 2017; 20(2):251–260. doi: https://doi.org/gt56x8

Karami N, Veshkini A, Asghari A, Mashhadi Rafiee S, Mortazavi P. The pathological and ultrasonographic evaluation of the chemical castration in dogs using calcium chloride injection. Arch. Razi. Inst. [Internet]. 2023; 78(5):1579–1585. doi: https://doi.org/gt56x9

Howaida M. Abu–Ahmed. Chemical Sterilization Of Dogs Using Single Bilateral Intra–Testicular Injection Of Calcium Chloride Or Clove Oil. Alex J. Vet. Sci. [Internet]. 2015; 45(1):26–32. doi: https://doi.org/gt56zb

Zedan IA, Al–Badrany MST. Control of stray dog population by single intratesticular injection of tannic acid. Iraqi J. Vet. Sci. [Internet]. 2021; 35(1):33–36. doi: https://doi.org/gt564r

Huuskonen V, Lynne Hughes JM, Estaca Bañon E, West E. Intratesticular lidocaine reduces the response to surgical castration in dogs. Vet. Anaesth. Analg. [Internet]. 2013; 40(1):74–82. doi: https://doi.org/f4gh7f

Pineda MH, Hepler DI. Chemical vasectomy in dogs. Long–term study. Theriogenology [Internet]. 1981; 16(1):1–11. doi: https://doi.org/crfr6k

Andrade Neto O, Gasperin BG, Rovani MT, Ilha GF, Nóbrega Jr. JE, Mondadori RG, Gonçalves PBD, Antoniazzi AQ. Intratesticular hypertonic sodium chloride solution treatment as a method of chemical castration in cattle. Theriogenology [Internet]. 2014; 82(7):1007–1011.e1. doi: https://doi.org/f7fwcr

Canpolat I, Karabulut E, Eröksüz Y. Chemical castration of adult and non–adult male dogs with sodium chloride solution. IOSR J. Agric. Vet. Sci. [Internet]. 2016 [cited 12 Feb. 2024]; 9(12):9–11. Available in: https://goo.su/WfrbLGJ

Oliveira ECS, Fagundes AKF, Melo CCS, Nery LTB, Rêvoredo RG, Andrade TFG, Oliveira–Esquerre K, Kastelic JP, Silva Jr. VA. Intratesticular injection of a zinc–based solution for contraception of domestic cats: a randomized clinical trial of efficacy and safety. Vet. J. [Internet]. 2013;197(2): 307–310. doi: https://doi.org/f5chx9

Brito LFC, Sertich PL, Rives W, Knobbe M, Del Piero F, Stull GB. Effects of intratesticular zinc gluconate treatment on testicular dimensions, echodensity, histology, sperm production, and testosterone secretion in American black bears (Ursus americanus). Theriogenology [Internet]. 2011; 75(8):1444–1452. doi: https://doi.org/bhwckq

Jana K, Samanta PK, Ghosh D. Evaluation of single intratesticular injection of calcium chloride for nonsurgical sterilization of male Black Bengal goats (Capra hircus): A dose–dependent study. Anim. Reprod. Sci. 2005; 86(1–2):89–108. doi: https://doi.org/brdhjv

Emir L, Dadalı M, Sunay M, Erol D, Çaydere M, Üstün H. Chemical castration with intratesticular injection of 20% hypertonic saline: A minimally invasive method. Urol. Oncol. [Internet]. 2008; 26(4):392–396. doi: https://doi.org/c8cxtk

Lymberopoulos AG, Kotsaki–Kovatsi VP, Taylor A, Papaioannou N, Brikas P. Effects of cadmium chloride administration on the macroscopic and microscopic characteristics of ejaculates from chios ram–lambs. Theriogenology [Internet]. 2000; 54(7):1145–1157. doi: https://doi.org/czwx9d

İbrahim A, Ali MM, Abou–Khalil NS, Ali MF. Evaluation of chemical castration with calcium chloride versus surgical castration in donkeys: testosterone as an end point marker. BMC Vet. Res. [Internet]. 2016; 12(46):1–9. doi: https://doi.org/f8cjpf

Steffens J, Kosharskyy B, Hiebl R, Schönberger B, Röttger P, Loening S. Paraffinoma of the external genitalia after autoinjection of vaseline. Europ. Urol. [Internet]. 2000; 38(6):778–781. doi: https://doi.org/fvjg3g

Santos P, Chaveiro A, Nunes G, Fonseca J, Cardoso J. Penile parafinoma. J. Eur. Acad. Dermatol. Venereol. [Internet]. 2003; 17(5):583–584. doi: https://doi.org/c62fvd

Brennan R, Overbye M, Van Hout MC, McVeigh J. “A slippery slope”: a scoping review of the self–injection of unlicensed oils and fillers as body enhancement. Perform. Enhanc. Health [Internet]. 2021; 8(4):100185. doi: https://doi.org/gnqwkt

Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal. Biochem. [Internet]. 1966; 16(2):359–364. doi: https://doi.org/b96rpj

Sedlak J, Lindsay RH. Estimation of total, protein–bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal. Biochem. [Internet]. 1968; 25;192–205. doi: https://doi.org/csbsfm

Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium–deficient rat liver. Biochem. Biophys Res. Commun. [Internet]. 1976; 71(4):952–958. doi: https://doi.org/d3vv59

Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121–126. doi: https://doi.org/dnf7v9

Türk G, Ateşşahin A, Sönmez M, Yüce A, Çeribaşi AO. Lycopene protects against cyclosporine A–induced testicular toxicity in rats. Theriogenology [Internet]. 2007; 67(4):778–785. doi: https://doi.org/fxghnh

Johnsen SG. Testicular biopsy score count – A method for registration of spermatogenesis in human testes: Normal values and results in 335 hypogonadal males. Hormones [Internet]. 1970; 1(1):2–25. doi: https://doi.org/bvxfrs

Aengwanich W, Sakundech K, Chompoosan C, Tuchpramuk P, Boonsorn T. Physiological changes, pain stress, oxidative stress, and total antioxidant capacity before, during, and after castration in male dogs. J. Vet. Behav. [Internet]. 2019; 32:76–79. doi: https://doi.org/ksq9

Paranzini CS, Sousa AK, Cardoso GS, Perencin FM, Trautwein LGC, Bracarense APFRL, Martins MIM. Effects of chemical castration using 20% CaCl2 with 0.5% DMSO in tomcats: Evaluation of inflammatory reaction by infrared thermography and effectiveness of treatment. Theriogenology [Internet]. 2018; 106:253–258. doi: https://doi.org/10/gt564s

İşler CT, Uyar A, Gökalp T. Ratlarda Skrotal Ekstrakutan ve Ensizyonel Subkutan Ligatür Uygulamasının ve Etkilerinin Değerlendirilmesi [Evaluation of Scrotal Extracutaneous and Incisional Subcutaneous Ligature Application and Its Effects in Rats]. Harran Üniv. Vet. Fak. Derg. [Internet]. 2019; 8(2):225–231. Turkish. doi: https://doi.org/gt564t

Zeng FM, Ding Y, Wassie T, Jing HJ, Ahmed S, Liu GQ, Jiang XP. Recent advances in immunocastration in sheep and goat and its animal welfare benefits: A review. J. Integr. Agric. [Internet]. 2022; 21(2):299–309. doi: https://doi.org/gt564v

Naz RK, Talwar GP. Immunological sterilization of male dogs by BCG. Int. J. Androl. [Internet]. 1981; 4(1‐6):111–128. doi: https://doi.org/b32xqn

Talwar GP, Naz RK, Das C, Das RP. A practicable immunological approach to block spermatogenesis without loss of androgens. PNAS [Internet]. 1979; 76(11):5882–5885. doi: https://doi.org/d9bnd4

Hassan A, Fromsa A. Review on chemical sterilization of male dogs. Int. J. Adv. Res. [Internet]. 2017; 5(11):758–770. doi: https://doi.org/gt564w

Silveira VCA, Colla ACN, de Arruda Mestieri ML. Chemical castration of small animals: Where are we? Pubvet [Internet]. 2021; 15(3):1–7. doi: https://doi.org/gt564x

Gerendai I, Csaba Z, Csernus V. Effect of intratesticular administration of somatostatin on testicular function in immature and adult rats. Life Sci. [Internet]. 1996; 59(10):859–866. doi: https://doi.org/brkxjx

Sancak T, Koşal V, Okulmuş C. Effect of castration and vasectomy on some oxidative stress parameters and blood hormone levels in rats. Arq. Bras. Med. Vet. Zootec. [Internet]. 2023; 75(4):651–656. doi: https://doi.org/gt564z

Raman G, Purandare TV, Munshi SR. Sterility induced in male rats by injection of chemical agents into the vas deferens. Androl. [Internet]. 1976; 8(4):321–325. doi: https://doi.org/c6j3dd

Tsuzuki N, Sasaki N, Kusano K, Endo Y, Torisu S. Oxidative stress markers in Thoroughbred horses after castration surgery under inhalation anesthesia. J. Equine Sci. [Internet]. 2016; 27(2):77–79. doi: https://doi.org/gt5642

Aydilek N, Aksakal M. Testosteronun Tavşanlarda Karaciğer Antioksidan Sistemi Üzerine Etkisi [Effects of testosterone on the liver antioxidant system in rabbits]. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi [Internet]. 2003 [cited 25 Feb. 2024]; 14(2):22–25. Turkish. Available in: https://goo.su/rWr3lI3

Bakır B, Gülyüz F, Karaca F, Yüksel H, Şahin A, Uslu BA. Köpeklerde Kimyasal Kastrasyon [Chemical castration in dogs]. Yüzüncü Yıl Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi [Internet]. 2006; 9(1):195–202. Turkish. Available in: https://goo.su/XjIgXU

Abshenas J, Molaei MM, Derakhshnfar A, Ghalekhani N. Chemical Sterilization by Intratesticular Injection of Eugenia Caryophyllata Essential Oil in Dog: A Histopathological Study. Iran J. Vet. Surg. [Internet]. 2013 [cited 25 Jan. 2024]; 8(2):9–16. Available in: https://goo.su/OUpX5nB

Kwak BK, Lee SH. Intratesticular injection of hypertonic saline: non–invasive alternative method for animal castration model. Dev. Reprod. [Internet]. 2013; 17(4):435–440. doi: https://doi.org/gb5dwh

Fesseha H, Negash G. Evaluation of single bilateral intratesticular injection of cetrimide for nonsurgical sterilization of adult male albino mice. Insights Vet. Sci. [Internet]. 2020; 4(1):025–034. doi: https://doi.org/gt5643