Efectos de la aplicación local de líquido amniótico bovino en la curación de fracturas en ratas (Rattus norvegicus)

  • Murat Tanrısever Firat University, Faculty of Veterinary Medicine, Department of Surgery, Elazig, Turkiye
  • Ozmen Istek Mus Alparslan University, Faculty of Health Medicine, Department of Nursing, Mus, Turkiye
  • Hatıce Eroksuz Firat University, Faculty of Veterinary Medicine, Department of Pathology, Elazig, Turkiye
  • Burak Karabulut Firat University, Faculty of Veterinary Medicine, Department of Pathology, Elazig, Turkiye
  • Erhan Cahıt Ozcan Firat University, Faculty of Medicine, Department of Esthetic, Plastic and Reconstructive Surgery, Elazig, Turkiye
  • Muhammet Bahattın Bıngul Harran University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Sanliurfa, Turkiye
  • Rıdvan Guler Dicle University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Diyarbakir, Turkiye
  • Serkan Dundar Firat University, Faculty of Dentistry, Department of Peridontology, Elazig, Turkiye
Palabras clave: Líquido amniótico bovino, fractura ósea, aplicación local, curación de fracturas óseas

Resumen

En este estudio, el objetivo fue examinar la aplicación local de líquido amniótico bovino en la curación de fracturas óseas en ratas. Veinte ratas hembra Sprague Dawley incluidas en el estudio se dividieron en 2 grupos de 10. El grupo simulado (n=10): se crearon fracturas óseas en la tibia derecha de las ratas y se fijaron con alambre de Kirschner. Después de un período de recuperación de cuatro semanas, los sujetos fueron sacrificados. Grupo de líquido amniótico bovino local (n = 10): se crearon fracturas óseas en los huesos de la tibia derecha de las ratas y se aplicó líquido amniótico bovino local durante la fijación con alambre de Kirschner. Después de un período de recuperación de cuatro semanas, los sujetos fueron sacrificados. Se descalcificaron muestras de todos los sujetos, se tiñeron con hematoxilina y eosina y se analizó la formación de hueso nuevo y la fibrosis. Cuando los grupos fueron evaluados en términos de regeneración ósea nueva, se determinó que la regeneración ósea nueva en los sujetos tratados con líquido amniótico bovino local fue estadísticamente significativamente mayor que en el grupo simulado (P<0,05). Cuando los grupos fueron evaluados en términos de fibrosis, se encontró que el valor de fibrosis en el grupo simulado era estadísticamente significativamente mayor en comparación con el grupo de líquido amniótico bovino local (P<0,05). Se puede afirmar que la aplicación local de líquido amniótico bovino puede afectar positivamente la curación de las fracturas óseas.

Descargas

La descarga de datos todavía no está disponible.

Citas

Acikan I, Dundar S. Biomechanical Examination of Osseointegration of Titanium Implants Placed Simultaneously With Allogeneic Bone Transfer. J. Craniofac. Surg. [Internet]. 2022; 33(1):350–353. doi: https://doi.org/gp5nmd

Padilla–Eguiluz NG, Gómez–Barrena E. Epidemiology of long bone non–unions in Spain. Injury. [Internet]. 2021; 52(Suppl4):S3–S7. doi: https://doi.org/mhsc

Wakefield SM, Giannoudis VP, Giannoudis PV. Reconstruction of a neglected hyperextension–bicondylar tibial plateau fracture 9 months after original injury and review of the literature. What outcomes can be expected? Trauma Case Rep. [Internet]. 2023; 45:e100823. doi: https://doi.org/mhss

Andrzejowski P, Giannoudis PV. The 'diamond concept' for long bone non–union management. J. Orthop Traumatol. [Internet]. 2019; 20(21):21–22. doi: https://doi.org/gh7zs5

Rodrigues M, Blattner C, Stuppia L. Amniotic Fluid Cells, Stem Cells, and p53: Can We Stereotype p53 Functions? Intern. J. Mol. Sci. [Internet]. 2019; 20(9):2236. doi: https://doi.org/mhsd

Fadhil E, Dosh RH, Wally ZJ, Haider J. Histological evaluation of the effects of bone morphogenetic protein 9 and angiopoietin 1 on bone healing. J. Taibah Univ. Med. Sci. [Internet]. 2023; 18(5):954–963. doi: https://doi.org/mhst

Mi J, Xu J, Yao H, Li X, Tong W, Li Y, Dai B, He X, Chow DHK, Li G, Lui KO, Zhao J, Qin L. Calcitonin Gene–Related Peptide Enhances Distraction Osteogenesis by Increasing Angiogenesis. Tissue Eng. Part A. [Internet]. 2021; 27(1–2):87–102. doi: https://doi.org/mhsf

Al Ruhaimi KA. Effect of calcium sulphate on the rate of osteogenesis in distracted bone, International J. Oral Maxillofacial Surgery. [Internet]. 2001; 30(3):228–233. doi: https://doi.org/fv8xnp

Akbulut Y, Gul M, Dundar S, Ozcan EC, Ozercan IH, Bozoglan A, Karasu N, Acikan I, Bingül MB. Evaluation of Effects of Systemic Zoledronic Acid Application on Bone Maturation in the Consolidation Period in Distraction Osteogenesis. J. Craniofac. Surg. [Internet]. 2021; 32(8):2901–2905. doi: https://doi.org/mhsg

Hagiwara T, Bell WH. Effect of electrical stimulation on mandibular distraction osteo–genesis. J. Craniomaxillofac. Surg. [Internet]. 2000; 28(1):12–19. doi: https://doi.org/cg9djh

Schortinghuis J, Bronckers AL, Gravendeel J, Stegenga B, Raghoebar GM. The effect of ultrasound on osteogenesis in the vertically distracted edentulous mandible: a double–blind trial. Intern. J. Oral Maxillofac. Surg. [Internet]. 2008; 37(11):1014–1021. doi: https://doi.org/cw8qsr

Hannouche D, Petite H, Sedel L. Current trends in the enhancement of fracture healing. J.Bone Joint Surg. Br. [Internet]. 2001; 83(2):15. doi: https://doi.org/bx48z8

Kerimoglu S, Livaoglu M, Sonmez B, Yulug E, Aynaci O, Topbas M, Yarar S. Effects of human amniotic fluid on fracture healing in rat tibia. J. Surg. Res. [Internet]. 2009; 152(2):281–287. doi: https://doi.org/dd5t62

Karaçal N, Koşucu P, Çobanoğlu Ü, Kutlu N. Effect of Human Amniotic Fluid on Bone Healing. J. Surg. Res. [Internet]. 2005; 129(2):283–287 doi: https://doi.org/bzr773

Gokce SM, Karacayli U, Nalcaci R, Avunduk MC, Özgöçmen M, Karasahin E, Gokce HS: The effect of human amniotic fluid on mandibular distraction osteogenesis. Intern. J. Oral Maxillofac. Surg. [Internet]. 2015; 44(3):404–411. doi: https://doi.org/f633kk

Dasari G, Prince I, Hearn MTW. Investigations into the rheological characteristics of bovine amniotic fluid. J. Biochem. Biophys. Meth. [Internet]. 1995; 30(4):217–225. doi: https://doi.org/dhbhbb

Ravelich SR, Breier BH, Reddy S,Keelan JA, Wells DN, Peterson AJ, Lee SF. Insulin–like growth factor–I and binding proteins 1, 2, and 3 in bovine nuclear transfer pregnancies. Biol. Reprod. [Internet]. 2004; 70(2):430–438. doi: https://doi.org/cdszx4

Decker M, Chiu ES, Dollbaum C, Moiin A, Hall J, Spendlove R, Longaker MT, Stern R. Hyaluronic acid–stimulating activity in sera from the bovine fetus and from breast cancer patients. Cancer Res. [Internet]. 1989; 49(13):3499–3505. Cited in PUBMED; PMID 2731171.

Tanrisever M, Eröksüz H, Bulut S. The comparison of the effects of intraarticular injections of bovine amniotic fluid and hyaluronic acid on cartilage tissue in an experimental osteoarthritic rabbit model: histopathological and immunohistochemical results. Turk. J. Vet. Anim. Sci. [Internet]. 2017; 41(2):273–281. doi: https://doi.org/mhsj

Lee HS,. Kim JC. Effect of amniotic fluid in corneal sensitivity and nerve regeneration after excimer laser ablation. Cornea. [Internet]. 1996; 15(5):517–524. Cited in PUBMED; PMID 8862929

Gunes N, Dundar S, Saybak A, Artas G, Acikan I, Ozercan I.H, Atilgan S, Yaman F. Systemic and local zoledronic acid treatment with hydroxyapatite bone graft: A histological and histomorphometric experimental study. Experim. Therap. Med. [Internet]. 2016;12(4):2417–2422. doi: https://doi.org/gbr3bs

Kim JS, Kim JC, Na BK, Jeong JM, Song CY. Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn. Exp. Eye Res. [Internet]. 2000; 70(3):329–337. doi: https://doi.org/fwqnf3

Sato H, Shimazaki J, Shinozaki N, Tsuboto K. Role of growth factors for ocular surface reconstruction after amniotic membrane transplantation. Invest. Ophthalmol. Vis. Sci. 1998; 39:428.

Piamo A, García M, Romero D, Ferrer D. Healing of a chronic ulcer of the lower limb of venous origin with fresh human amniochorionic membrane allograft. Biomed. [Internet]. 2022; 42(Suppl 1):17–25. doi: https://doi.org/mhsk

Marangon FB, Alfonso EC, Miler D, Remonda NM, Mualem MS, Tseng SC. Incidence of microbial infection after amniotic membrane. Cornea. [Internet]. 2004; 23(3):264–269. doi: https://doi.org/fxf6zm

Dasari G, Prince I, Hearn MTW. Investigations into the rheological characteristics of bovine amniotic fluid. J. Biochem. Biophys. Meth. [Internet]. 1995; 30(4):217–225. doi: https://doi.org/dhbhbb

Ravelich SR, Breier BH, Reddy S, Keelan JA, Wells DN, Pteterson AJ, Lee RSF. Insulin–like growth factor–1 and binding proteins 1, 2, and 3 in bovine nuclear transfer pregnancies. Biol. Reprod. [Internet]. 2004; 70(2):430–438. doi: https://doi.org/cdszx4

Longaker MT, Adzick NS, Hall JL, Stair SE, Crombleholme TM, Duncan BW, Bradley SM, Harrison MR, Stern R. Studies in fetal wound healing, VII. Fetal wound healing may be modulated by hyaluronic acid stimulating activity in amniotic fluid. J. Pediatr. Surg. [internet]. 1990; 25(4):430–433. doi: https://doi.org/dhb9b5

Decker M, Chiu ES, Dolbaum C, Moiin A, Hall J, Longaker MT, Spendlove R, Stern R. Hyaluronic acid stimulating factor from the bovine fetus and from breast cancer patients. Cancer Res. [Internet]. 1989; 49(13):3499–3505. Cited in PUBMED; PMID 2731171.

Publicado
2024-02-28
Cómo citar
1.
Tanrısever M, Istek O, Eroksuz H, Karabulut B, Ozcan EC, Bıngul MB, Guler R, Dundar S. Efectos de la aplicación local de líquido amniótico bovino en la curación de fracturas en ratas (Rattus norvegicus). Rev. Cient. FCV-LUZ [Internet]. 28 de febrero de 2024 [citado 19 de abril de 2024];34(1):6. Disponible en: https://produccioncientificaluz.org/index.php/cientifica/article/view/41700
Sección
Medicina Veterinaria